FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617420
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT286550.6205846555002577TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC250.00502541256.9908872
TCGCGGC250.00502541256.9908872
CGAGCGC851.2732926E-1050.3782771
CGACGGG957.2759576E-1250.083671
CGCGGCG405.345634E-447.5794831
CGACGTG901.1803422E-842.2928731
CGGTTCG459.549198E-442.2928731
CGCGTGA459.549198E-442.2928731
CGGGTCG704.263762E-640.7824131
CGACGCC704.263762E-640.7824131
CGAGGCG1500.038.0635871
CCGATGG1002.989873E-838.0635831
CGTGCGG500.001603099938.0635831
CGGGATG2000.038.0635831
GTCCGGC1002.989873E-838.0635831
AACGCGG651.3157989E-436.59961
CGATGCG1054.5945853E-836.2510341
CGGGGCG1850.036.0060961
AGGCCGC1353.0377123E-1035.244061
GTCGCGG550.002559357834.603261
Produced by FastQC (version 0.11.2)