##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5221056 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64141238860491 33.0 33.0 33.0 33.0 33.0 2 32.65010890517168 33.0 33.0 33.0 33.0 33.0 3 32.71408791631425 33.0 33.0 33.0 33.0 33.0 4 36.64947225235661 37.0 37.0 37.0 37.0 37.0 5 36.61616098352517 37.0 37.0 37.0 37.0 37.0 6 36.52167875617499 37.0 37.0 37.0 37.0 37.0 7 36.419624497419676 37.0 37.0 37.0 37.0 37.0 8 36.36958596115422 37.0 37.0 37.0 37.0 37.0 9 36.424519675713114 37.0 37.0 37.0 37.0 37.0 10-11 36.357707042406744 37.0 37.0 37.0 37.0 37.0 12-13 36.3450251251854 37.0 37.0 37.0 37.0 37.0 14-15 38.197448370597826 40.0 37.0 40.0 37.0 40.0 16-17 38.219573779710466 40.0 37.0 40.0 37.0 40.0 18-19 38.154496236010495 40.0 37.0 40.0 37.0 40.0 20-21 38.14675105955577 40.0 37.0 40.0 37.0 40.0 22-23 37.955781359173315 40.0 37.0 40.0 33.0 40.0 24-25 37.98256607475576 40.0 37.0 40.0 33.0 40.0 26-27 37.92084273756114 40.0 37.0 40.0 33.0 40.0 28-29 37.721576918539085 40.0 37.0 40.0 33.0 40.0 30-31 37.71466835444784 40.0 37.0 40.0 33.0 40.0 32-33 37.55642325996887 40.0 37.0 40.0 33.0 40.0 34-35 37.38058258712414 40.0 37.0 40.0 33.0 40.0 36-37 37.07337174701823 40.0 37.0 40.0 33.0 40.0 38-39 37.23300956741318 40.0 37.0 40.0 33.0 40.0 40-41 36.99492545186261 38.5 37.0 40.0 33.0 40.0 42-43 36.79837373895243 37.0 37.0 40.0 33.0 40.0 44-45 36.814259988783874 37.0 37.0 40.0 33.0 40.0 46-47 36.815095739252754 37.0 37.0 40.0 33.0 40.0 48-49 36.4870384458623 37.0 37.0 40.0 33.0 40.0 50-51 36.417753036933526 37.0 37.0 40.0 33.0 40.0 52-53 36.34669509386607 37.0 37.0 40.0 33.0 40.0 54-55 35.95583144482649 37.0 37.0 40.0 30.0 40.0 56-57 35.86461177585531 37.0 37.0 40.0 30.0 40.0 58-59 35.61397550610451 37.0 35.0 40.0 27.0 40.0 60-61 35.3268182720124 37.0 33.0 40.0 27.0 40.0 62-63 35.058828520513856 37.0 33.0 38.5 27.0 40.0 64-65 34.81542287613847 37.0 33.0 37.0 27.0 40.0 66-67 34.21715712300347 37.0 33.0 37.0 27.0 40.0 68-69 34.036693534794495 37.0 33.0 37.0 27.0 40.0 70-71 33.51698861303154 37.0 33.0 37.0 27.0 40.0 72-73 32.84620352664288 37.0 33.0 37.0 22.0 37.0 74-75 32.050794513600316 37.0 33.0 37.0 18.5 37.0 76-77 29.55443304955932 33.0 27.0 35.0 15.0 37.0 78-79 31.60615898392969 33.0 33.0 37.0 15.0 37.0 80-81 31.888470071954792 37.0 33.0 37.0 15.0 37.0 82-83 31.823918092431875 37.0 33.0 37.0 15.0 37.0 84-85 31.681235271178856 35.0 33.0 37.0 15.0 37.0 86-87 31.54152627361208 33.0 33.0 37.0 15.0 37.0 88-89 31.33598002013386 33.0 33.0 37.0 15.0 37.0 90-91 31.09098427597789 33.0 33.0 37.0 15.0 37.0 92-93 31.08594698084066 33.0 33.0 37.0 15.0 37.0 94-95 31.014502717457923 33.0 33.0 37.0 15.0 37.0 96-97 30.84553297264002 33.0 33.0 37.0 15.0 37.0 98-99 30.673799610653475 33.0 33.0 37.0 15.0 37.0 100-101 29.12282974938403 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.17938804438293943 2306 2 0.16023460432066372 2306 3 0.13531885256869458 2306 4 0.15336518070273542 2306 5 0.17872655924911385 2306 6 0.19464411430899986 2306 7 0.15376758947397917 2306 8 0.17431341056334304 2306 9 0.2027912190344594 2306 10-11 0.24506317066241934 2306 12-13 0.22683866144418374 2306 14-15 0.43998288294077526 2306 16-17 0.4306235012079682 2306 18-19 0.38006023299850256 2306 20-21 0.3864383306610648 2306 22-23 0.4461040550998021 2306 24-25 0.427154492949974 2306 26-27 0.4411754325101853 2306 28-29 0.5054062732477647 2306 30-31 0.4406854548999348 2306 32-33 0.439136134256465 2306 34-35 0.38345377351799215 2306 36-37 0.4937505142029437 2306 38-39 0.5027703605733791 2306 40-41 0.46681939259285343 2306 42-43 0.3819560341755377 2306 44-45 0.562792726770958 2306 46-47 0.5591445014485146 2306 48-49 0.6158786972004506 2306 50-51 0.4756865915551458 2306 52-53 0.3566099698661773 2306 54-55 0.45652011886403443 2306 56-57 0.5612923188006746 2306 58-59 0.520732342197924 2306 60-61 0.5041360518371292 2306 62-63 0.5458587328539082 2306 64-65 0.30828930979351554 2306 66-67 0.3389195674782073 2306 68-69 0.42293344390675713 2306 70-71 0.4696655732056314 2306 72-73 0.2726792471207986 2306 74-75 0.26755863858210915 2306 76-77 -0.0035638864079210464 2306 78-79 0.5123960437423527 2306 80-81 0.521871667346776 2306 82-83 0.5051681496719738 2306 84-85 0.5586764457012237 2306 86-87 0.48015162402680645 2306 88-89 0.6147537607358942 2306 90-91 0.5028328605983532 2306 92-93 0.44211278807971155 2306 94-95 0.5756563152460537 2306 96-97 0.5034489437346146 2306 98-99 0.3779462838179093 2306 100-101 0.3872557611051839 2307 1 0.1339364160159846 2307 2 0.11902187667330821 2307 3 0.12713938246567125 2307 4 0.12140945670945769 2307 5 0.16643725799816167 2307 6 0.16116389804868447 2307 7 0.15299532519715342 2307 8 0.20760516610988589 2307 9 0.2244642609285421 2307 10-11 0.2358669636810049 2307 12-13 0.22286794791613573 2307 14-15 0.36464698356719083 2307 16-17 0.3001466645010211 2307 18-19 0.3650147760470617 2307 20-21 0.37344725099529796 2307 22-23 0.38965214652387914 2307 24-25 0.37684274926371586 2307 26-27 0.3793847207943699 2307 28-29 0.431147159802606 2307 30-31 0.3612355975014765 2307 32-33 0.3371711427330055 2307 34-35 0.4747262661187719 2307 36-37 0.44979460958403905 2307 38-39 0.31255919306493496 2307 40-41 0.3187138833018395 2307 42-43 0.4062280515873624 2307 44-45 0.4288877113295655 2307 46-47 0.34016448599963667 2307 48-49 0.47495978524856497 2307 50-51 0.2889692440484879 2307 52-53 0.1671695414843839 2307 54-55 0.45062110713144676 2307 56-57 0.4288370333628393 2307 58-59 0.4908271975673344 2307 60-61 0.3895123736713231 2307 62-63 0.3121781238225694 2307 64-65 0.36144184006251123 2307 66-67 0.20650498261661454 2307 68-69 0.32578930256182304 2307 70-71 0.37456049783480694 2307 72-73 0.324535698790136 2307 74-75 0.39165810468983153 2307 76-77 0.11465262690140676 2307 78-79 0.501413108378518 2307 80-81 0.672576720348733 2307 82-83 0.584550302364633 2307 84-85 0.5898202515131175 2307 86-87 0.4741071249294784 2307 88-89 0.6889129917970394 2307 90-91 0.6469819808109882 2307 92-93 0.6483033726398695 2307 94-95 0.5606236874990245 2307 96-97 0.5439944929426872 2307 98-99 0.471093993416158 2307 100-101 0.4660586557804045 2201 1 0.16139921149774494 2201 2 0.1728721215244775 2201 3 0.12541547216720517 2201 4 0.11686091563520762 2201 5 0.19256557954120268 2201 6 0.17846045134501765 2201 7 0.18014142798394772 2201 8 0.09692155608809827 2201 9 0.12536212968235816 2201 10-11 0.21146132094919778 2201 12-13 0.1158437738216449 2201 14-15 0.30697606387819576 2201 16-17 0.3043204897839118 2201 18-19 0.31692033071320225 2201 20-21 0.26278217056056263 2201 22-23 0.35337307662323525 2201 24-25 0.30287936796634085 2201 26-27 0.3063797229609264 2201 28-29 0.31530473518179747 2201 30-31 0.31485148746723723 2201 32-33 0.418415964400765 2201 34-35 0.42580068145588257 2201 36-37 0.38677442436263476 2201 38-39 0.21833120239101333 2201 40-41 0.3590633908358285 2201 42-43 0.43673055852308096 2201 44-45 0.3314088807383868 2201 46-47 0.36502907252206995 2201 48-49 0.5100050194931924 2201 50-51 0.34031561759064033 2201 52-53 0.35614314842172945 2201 54-55 0.44352355930892884 2201 56-57 0.5428405611441889 2201 58-59 0.48063629699790766 2201 60-61 0.4462251962887578 2201 62-63 0.4974473025895563 2201 64-65 0.4164512294367242 2201 66-67 0.43230117491534514 2201 68-69 0.33847977855455014 2201 70-71 0.45769505098965624 2201 72-73 0.21644491355090878 2201 74-75 0.6072239888274993 2201 76-77 0.40929307988250585 2201 78-79 0.7587567674936828 2201 80-81 0.7238758677872603 2201 82-83 0.6374532471370102 2201 84-85 0.6267178143998748 2201 86-87 0.6320864319343791 2201 88-89 0.7683971876630835 2201 90-91 0.7612077204407939 2201 92-93 0.6679844002615596 2201 94-95 0.540904311937517 2201 96-97 0.7007736726748135 2201 98-99 0.6004424822524363 2201 100-101 0.6493902755326602 1101 1 0.10453003253580562 1101 2 0.13241154679715095 1101 3 0.12079625908262415 1101 4 0.1645089457131803 1101 5 0.16282608382955743 1101 6 0.12091075676815422 1101 7 0.16124698889807831 1101 8 0.2015831613478909 1101 9 0.1774666522316508 1101 10-11 0.1814868541589476 1101 12-13 0.17582477509058236 1101 14-15 0.2900850568988531 1101 16-17 0.2435138698821433 1101 18-19 0.2836288345877449 1101 20-21 0.32510550050283626 1101 22-23 0.25394706468609485 1101 24-25 0.28902811607257917 1101 26-27 0.30349685086043365 1101 28-29 0.24145148749982326 1101 30-31 0.2064863914157371 1101 32-33 0.21146057963903075 1101 34-35 0.2703564492079167 1101 36-37 0.30359591906455563 1101 38-39 0.33037389948413676 1101 40-41 0.3685689866605486 1101 42-43 0.3771137938392286 1101 44-45 0.42278857300690476 1101 46-47 0.4483074195863992 1101 48-49 0.5467449308544303 1101 50-51 0.37846833968189486 1101 52-53 0.42862944871257014 1101 54-55 0.5948589128280943 1101 56-57 0.44462356650490875 1101 58-59 0.47034031773106477 1101 60-61 0.3925335194952524 1101 62-63 0.33327230840230726 1101 64-65 0.30775278649184656 1101 66-67 0.32621456011509053 1101 68-69 0.5022750389554389 1101 70-71 0.4711418049882923 1101 72-73 0.3115275468793257 1101 74-75 0.48428398440974973 1101 76-77 0.15384491050773264 1101 78-79 0.565171671654749 1101 80-81 0.6672547487563385 1101 82-83 0.5809658700000995 1101 84-85 0.47213770818854783 1101 86-87 0.5630417406760664 1101 88-89 0.5796702811761776 1101 90-91 0.6212226532507401 1101 92-93 0.4323527272700929 1101 94-95 0.38715893420030767 1101 96-97 0.4568815202114642 1101 98-99 0.4444280210600233 1101 100-101 0.4744655900983581 2304 1 0.12258253932018448 2304 2 0.12661899064195126 2304 3 0.09544909912659705 2304 4 0.10276720255635752 2304 5 0.17316958089698886 2304 6 0.10454185523768444 2304 7 0.12448066286478365 2304 8 0.16778568759932 2304 9 0.1246782973813012 2304 10-11 0.13128797319794216 2304 12-13 0.1743410761446782 2304 14-15 0.3117169701192992 2304 16-17 0.2537520689015551 2304 18-19 0.2936513471358779 2304 20-21 0.21477248960841422 2304 22-23 0.1636209010735783 2304 24-25 0.2995183613864896 2304 26-27 0.2681236878132367 2304 28-29 0.19685587345439615 2304 30-31 0.1612271378770913 2304 32-33 0.34049097514228066 2304 34-35 0.37236531489632085 2304 36-37 0.38660190490953283 2304 38-39 0.38192914729015115 2304 40-41 0.3823564861824167 2304 42-43 0.3537816952854911 2304 44-45 0.37935021941460434 2304 46-47 0.3234138993286706 2304 48-49 0.3185954029721927 2304 50-51 0.24700401761297996 2304 52-53 0.28666971823484744 2304 54-55 0.44658631712504615 2304 56-57 0.36281738210620773 2304 58-59 0.25731843973456137 2304 60-61 0.30366996613117436 2304 62-63 0.3114624502070811 2304 64-65 0.2852065691569621 2304 66-67 0.2945029767044147 2304 68-69 0.18207858246745445 2304 70-71 0.25698259172177984 2304 72-73 0.0609493166108237 2304 74-75 0.23344912650902927 2304 76-77 0.20091146541884441 2304 78-79 0.455923056127741 2304 80-81 0.6199489462085133 2304 82-83 0.51921903646803 2304 84-85 0.49705800382008647 2304 86-87 0.4865296192563626 2304 88-89 0.5143856368457875 2304 90-91 0.6585910252367491 2304 92-93 0.39025685107034747 2304 94-95 0.32600571114195276 2304 96-97 0.39548934039497396 2304 98-99 0.26492851838968434 2304 100-101 0.2171372313705966 2202 1 0.12610370957281702 2202 2 0.15016607928860992 2202 3 0.13235229994410247 2202 4 0.036395724256628625 2202 5 0.11295618691841014 2202 6 0.1104396310563871 2202 7 0.07417558388087997 2202 8 0.12836710839335552 2202 9 0.166619951774571 2202 10-11 0.09985367058415306 2202 12-13 0.11514732365935032 2202 14-15 0.1704597869780784 2202 16-17 0.1766185070424271 2202 18-19 0.14706249464470744 2202 20-21 0.11928339906032193 2202 22-23 0.13148363184619427 2202 24-25 0.18800110202943898 2202 26-27 0.10148239292960426 2202 28-29 0.062480435036441406 2202 30-31 0.1554847267824897 2202 32-33 0.24120300738660916 2202 34-35 0.2792518390090635 2202 36-37 0.3371447412361377 2202 38-39 0.26447413024013144 2202 40-41 0.30298181928788637 2202 42-43 0.3474212588963539 2202 44-45 0.14775291980190985 2202 46-47 0.2556487934621856 2202 48-49 0.32093294843630815 2202 50-51 0.2684169498573681 2202 52-53 0.2857578319255083 2202 54-55 0.3319715801610741 2202 56-57 0.47016493525237735 2202 58-59 0.46240542923170125 2202 60-61 0.4118237935234106 2202 62-63 0.3294832833675727 2202 64-65 0.34621353996794113 2202 66-67 0.36238830472539263 2202 68-69 0.3306445213580034 2202 70-71 0.422859875620091 2202 72-73 0.16450389189224524 2202 74-75 0.3306231016318648 2202 76-77 0.05612460867535063 2202 78-79 0.48703814898800957 2202 80-81 0.6398027236222426 2202 82-83 0.5905320670286471 2202 84-85 0.5018211931375589 2202 86-87 0.4508924727168697 2202 88-89 0.5730735898162251 2202 90-91 0.6655830600177985 2202 92-93 0.5168768889625639 2202 94-95 0.3702225070134446 2202 96-97 0.300787673849058 2202 98-99 0.23887086761492426 2202 100-101 0.3621400593680697 1102 1 0.10260449443705255 1102 2 0.13425989836511576 1102 3 0.08457955047213517 1102 4 0.026731767460240974 1102 5 0.09674611730027749 1102 6 0.07712220532741298 1102 7 0.068698092560048 1102 8 0.06380744982237019 1102 9 0.07414872228640235 1102 10-11 0.10544432493050238 1102 12-13 0.1210445683205208 1102 14-15 0.18207527217685282 1102 16-17 0.10969140128637633 1102 18-19 0.1401990721765003 1102 20-21 0.1349184157528498 1102 22-23 0.18952314800868209 1102 24-25 0.15261815695669156 1102 26-27 0.1628977577929973 1102 28-29 0.24234197438823912 1102 30-31 0.18858706868629582 1102 32-33 0.14988362673042133 1102 34-35 0.17367905893035385 1102 36-37 0.14630425325729846 1102 38-39 0.07938225423856693 1102 40-41 0.22378988085274898 1102 42-43 0.22764070191639973 1102 44-45 0.13759286710811836 1102 46-47 0.2214400945241195 1102 48-49 0.14619193794670338 1102 50-51 0.19000351377648173 1102 52-53 0.17195918489689177 1102 54-55 0.1933483990050604 1102 56-57 0.26305215324244813 1102 58-59 0.14900602937483853 1102 60-61 0.06359528072871257 1102 62-63 0.1326695567666647 1102 64-65 0.16208422508835696 1102 66-67 0.2504793102823655 1102 68-69 0.16761869456637157 1102 70-71 0.1404150296316189 1102 72-73 0.09326530536596778 1102 74-75 0.09492922622222721 1102 76-77 -0.10813786296199268 1102 78-79 0.3625871879258433 1102 80-81 0.5113331897057556 1102 82-83 0.40672250196858784 1102 84-85 0.3467359509207455 1102 86-87 0.3042565475827743 1102 88-89 0.4192197199283463 1102 90-91 0.5750380421365584 1102 92-93 0.4849814740825984 1102 94-95 0.34721215440495357 1102 96-97 0.1887810724837493 1102 98-99 0.19087732605581564 1102 100-101 0.22573364471085355 2305 1 0.14112094817123477 2305 2 0.12489474759144059 2305 3 0.10404790239634565 2305 4 0.06325818425734298 2305 5 0.07007768032211459 2305 6 -6.621244863893594E-4 2305 7 0.028016573106143028 2305 8 0.09957244615598171 2305 9 0.07281975433164689 2305 10-11 0.032684975188352894 2305 12-13 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-1.723716716357096 1213 98-99 -1.698500463673458 1213 100-101 -1.5540271693447387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 34.0 9 77.0 10 86.0 11 52.0 12 81.0 13 228.0 14 651.0 15 1702.0 16 3357.0 17 5583.0 18 7289.0 19 9761.0 20 12887.0 21 16706.0 22 21666.0 23 28287.0 24 36797.0 25 47335.0 26 64277.0 27 94682.0 28 154349.0 29 193215.0 30 132984.0 31 121313.0 32 159060.0 33 221967.0 34 338720.0 35 579094.0 36 1080335.0 37 1513787.0 38 374137.0 39 555.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.07286722435089 24.303510332866367 20.403974080482058 15.219648362300688 2 29.55563012539992 28.082805470770666 28.657459333897208 13.704105069932213 3 34.22003134997978 18.755209674058275 30.610589122200565 16.414169853761386 4 28.78767592056789 18.63763403848095 29.05553739036959 23.51915265058157 5 36.86384516848699 17.401498853871708 28.942937980362593 16.79171799727871 6 32.80282762720798 17.833461276799177 32.437384314590766 16.926326781402075 7 30.92493166133441 25.061213670184728 25.51240591941554 18.501448749065325 8 28.230744891454908 30.59473026146435 23.14489635813138 18.029628488949363 9 33.8713854055578 22.607859406219738 24.455186843427843 19.065568344794617 10-11 26.416754198662773 29.334691707329778 27.86808066274554 16.38047343126191 12-13 22.786692960198092 28.116764118216697 27.529469134213464 21.567073787371747 14-15 22.297117671214405 28.395788131749594 27.734121219921793 21.57297297711421 16-17 25.57342023811348 24.887809708410586 28.12854335534683 21.410226698129108 18-19 21.267105734931786 24.852989510168058 31.853536909008444 22.026367845891713 20-21 24.814175523112567 25.16878769352407 31.389138519104183 18.627898264259183 22-23 21.147800368354602 32.417560738670495 27.5010266122409 18.933612280734014 24-25 21.018726862918154 24.850355943318746 31.458961558734476 22.671955635028624 26-27 20.95409434413268 25.08173633839591 27.796809687542133 26.167359629929273 28-29 24.34198177533434 29.244907543608033 27.26752021047083 19.145590470586793 30-31 20.510352694933744 26.14631982495495 30.765356663479572 22.577970816631733 32-33 20.28814668909891 29.8672816380441 27.24758171526986 22.596989957587123 34-35 24.06084363592764 29.928364101856413 27.401853897166962 18.60893836504898 36-37 20.275744983390336 29.901699962612927 31.327244909841994 18.495310144154743 38-39 20.421083922489654 26.110828968151324 31.069692333078176 22.39839477628085 40-41 23.984383065782016 26.01260969942401 30.782122391391415 19.22088484340256 42-43 20.52177510605156 29.514248186744595 30.673295118539762 19.290681588664082 44-45 20.269265101809744 25.928203059631333 30.91257527190493 22.889956566653996 46-47 23.884415898349616 25.941958558190652 27.37322720744541 22.800398336014318 48-49 20.323629368663664 29.75563784656361 30.635684469266895 19.285048315505833 50-51 23.869427248747936 26.235173919314402 30.211474026022533 19.68392480591513 52-53 20.301714135543904 26.042800666151223 26.855923488956012 26.79956170934886 54-55 24.02296907364615 26.12014702889094 30.453121969325082 19.403761928137833 56-57 20.55954585750144 26.028882474392702 30.29719168631485 23.114379981791007 58-59 20.480704571013657 26.270952155612566 30.164628527309407 23.083714746064377 60-61 23.844779182403407 26.314711581309254 30.09350277734246 19.747006458944874 62-63 20.218268093335386 26.330807173595126 30.220889486489483 23.23003524658 64-65 24.104197088996123 26.341537329263804 29.993596105635334 19.56066947610474 66-67 20.718349582786384 33.18480467322162 26.390197989680964 19.706647754311028 68-69 20.845290877366583 33.22833333253507 26.184370409502876 19.74200538059547 70-71 20.731204051522262 33.40536354630047 26.15428591900979 19.70914648316748 72-73 20.45006370326053 33.4517071238167 26.140808487816493 19.95742068510627 74-75 20.75639143481874 32.784526920064856 26.055365691855602 20.403715953260797 76-77 20.74104323612758 32.15201434147446 26.280978023571777 20.825964398826184 78-79 20.695040644293346 31.574923862292643 26.67708177182586 21.052953721588146 80-81 20.60460099448968 31.255417275556468 26.866401676293016 21.273580053660837 82-83 20.249629523363154 31.17953785278119 27.006793939142042 21.56403868471361 84-85 20.36257247996975 30.95331793363932 27.008275667484217 21.675833918906715 86-87 20.2503888917254 30.877078603401902 27.45830867087494 21.414223833997763 88-89 20.344390620238954 30.940565298800017 27.48564328642418 21.229400794536847 90-91 20.58678948743708 30.438275053237916 27.205647395269395 21.769288064055615 92-93 20.341899486942868 30.791312854672263 27.06091584592631 21.80587181245856 94-95 20.298272373302265 30.711994512064262 26.980533540791612 22.00919957384186 96-97 20.561676637987226 30.940190853106415 26.788122243165844 21.710010265740515 98-99 20.707049417045035 30.689961616638513 26.841182710319057 21.761806255997403 100-101 20.726020714543292 30.760113831500714 26.39031052653967 22.123554927416322 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2069.0 1 1261.5 2 536.0 3 768.5 4 804.0 5 600.5 6 502.5 7 529.0 8 562.0 9 544.0 10 521.5 11 515.0 12 507.5 13 509.0 14 541.0 15 564.0 16 590.0 17 630.0 18 677.5 19 765.0 20 908.5 21 1172.0 22 1603.5 23 2271.5 24 3253.5 25 4989.0 26 8283.0 27 13118.5 28 18187.5 29 23269.5 30 28845.5 31 33861.0 32 44399.0 33 65543.0 34 96527.0 35 131652.0 36 176933.5 37 235944.0 38 283970.5 39 301293.5 40 309052.0 41 312533.5 42 310563.5 43 311492.5 44 299550.0 45 278752.0 46 257390.0 47 227275.5 48 193866.0 49 169891.5 50 150416.5 51 135871.5 52 125118.5 53 109700.0 54 91817.0 55 76087.5 56 63188.0 57 53791.0 58 47073.0 59 40284.5 60 33030.0 61 27149.0 62 22254.0 63 18043.0 64 14810.5 65 11731.0 66 8962.0 67 7275.0 68 6512.0 69 5252.5 70 3636.5 71 2564.5 72 1833.0 73 1348.5 74 1037.0 75 800.0 76 632.0 77 475.5 78 322.0 79 223.0 80 139.0 81 84.5 82 66.5 83 53.0 84 34.0 85 19.5 86 15.5 87 12.5 88 10.0 89 9.5 90 8.5 91 4.0 92 0.0 93 1.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17975290822393017 2 0.0 3 0.0 4 0.00856148641194425 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0020685470525502888 12-13 0.0 14-15 0.0 16-17 3.064514151926354E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 9.576606724769856E-6 36-37 0.0 38-39 0.0019440511651282804 40-41 0.00599495580970593 42-43 0.0024611879282658527 44-45 0.0012928419078439305 46-47 0.006473786145944423 48-49 0.008111385895880067 50-51 0.00522882727172434 52-53 0.006119451697127937 54-55 0.019028717562117704 56-57 0.02123133710881477 58-59 0.02731248237904363 60-61 0.025243935326493338 62-63 0.010524690790522071 64-65 0.015226804692384069 66-67 0.022754017578053176 68-69 0.026306938672942794 70-71 0.028480828399465547 72-73 0.022035772073695437 74-75 0.03195713664055701 76-77 0.02890219909535542 78-79 0.02056097463808088 80-81 0.00936592137682492 82-83 0.001513103862513637 84-85 0.007460176638595716 86-87 0.027111373637823462 88-89 0.0246214558893833 90-91 0.02091530908689736 92-93 0.04293192794714326 94-95 0.04474190661812476 96-97 0.03359473639049265 98-99 0.040758038220620504 100-101 0.04048989323232695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5221056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.977555522979 #Duplication Level Percentage of deduplicated Percentage of total 1 90.73573325617734 48.06957346497347 2 4.962558666326781 5.258084545627353 3 1.2555176604986027 1.9954276970743625 4 0.6265795872635047 1.3277861949527034 5 0.418774562325814 1.109282631361352 6 0.3147001983125175 1.0003228337516339 7 0.20174165910117786 0.7481445962431388 8 0.16108835578202266 0.6827253850038001 9 0.1271335713714697 0.6061703252549985 >10 0.9602205756247169 9.760239563908286 >50 0.11543904494144923 4.420996827545851 >100 0.10414370017312301 11.82725558172779 >500 0.013816358305219717 4.846395055841217 >1k 0.0024808255074639267 1.9807013996611913 >5k 3.599006824508634E-5 0.10795541978888495 >10k+ 3.598822058585166E-5 6.2589384772839205 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 328283 6.287674370855245 TruSeq Adapter, Index 7 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5662 0.10844549455129385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027389095232841785 0.0 0.0 2 0.0030645141519263534 0.0 0.0 3 0.0034475784209171477 0.0 0.0 4 0.0038881023302565613 0.0 0.0 5 0.004366932666495054 0.0 0.0 6 0.00528628691207296 0.0 0.0 7 0.006263100797999485 0.0 0.0 8 0.007144148616678312 0.0 0.0 9 0.008216728569852536 0.0 0.0 10-11 0.011961181799237549 0.0 1.915321344953971E-5 12-13 0.01577267127569595 0.0 1.915321344953971E-5 14-15 0.02047478517755795 0.0 1.915321344953971E-5 16-17 0.02508113301217225 0.0 1.915321344953971E-5 18-19 0.030645141519263536 0.0 2.8729820174309567E-5 20-21 0.035577093982520014 0.0 5.745964034861913E-5 22-23 0.04079634464751958 0.0 5.745964034861913E-5 24-25 0.04656146189583103 0.0 5.745964034861913E-5 26-27 0.05268091359295897 0.0 5.745964034861913E-5 28-29 0.05973887274911435 0.0 5.745964034861913E-5 30-31 0.08948381323624952 0.0 5.745964034861913E-5 32-33 0.09842836391718457 0.0 5.745964034861913E-5 34-35 0.10700900354257836 0.0 5.745964034861913E-5 36-37 0.11599186065041248 0.0 5.745964034861913E-5 38-39 0.12594195503744837 0.0 5.745964034861913E-5 40-41 0.1366964843893649 0.0 5.745964034861913E-5 42-43 0.1477574651564741 0.0 5.745964034861913E-5 44-45 0.1596707639220878 0.0 5.745964034861913E-5 46-47 0.17348023081920594 0.0 5.745964034861913E-5 48-49 0.18852507998381937 0.0 5.745964034861913E-5 50-51 0.20354119932825848 0.0 5.745964034861913E-5 52-53 0.2198693137939911 0.0 5.745964034861913E-5 54-55 0.23809359639122812 0.0 5.745964034861913E-5 56-57 0.25635618541536426 0.0 5.745964034861913E-5 58-59 0.2768597004130965 0.0 7.661285379815885E-5 60-61 0.2991923472952598 0.0 7.661285379815885E-5 62-63 0.32344031552237706 0.0 7.661285379815885E-5 64-65 0.34977598401549415 0.0 7.661285379815885E-5 66-67 0.3777109458316479 0.0 7.661285379815885E-5 68-69 0.40884449429387465 0.0 7.661285379815885E-5 70-71 0.44275525870628474 0.0 8.61894605229287E-5 72-73 0.47879202981159363 0.0 9.576606724769854E-5 74-75 0.5172995654518933 0.0 9.576606724769854E-5 76-77 0.5607869365890732 0.0 9.576606724769854E-5 78-79 0.606773802081418 0.0 9.576606724769854E-5 80-81 0.6581139907329092 0.0 9.576606724769854E-5 82-83 0.71654086836073 0.0 9.576606724769854E-5 84-85 0.780838205910835 0.0 9.576606724769854E-5 86-87 0.8516476360337832 0.0 9.576606724769854E-5 88-89 0.9278199659226026 0.0 9.576606724769854E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 44065 0.0 81.43621 3 GATCGGA 44220 0.0 81.0397 1 ATCGGAA 44250 0.0 80.63424 2 CGGAAGA 44330 0.0 80.585144 4 GAAGAGC 46125 0.0 77.642494 6 GGAAGAG 49770 0.0 72.00391 5 AGAGCAC 52320 0.0 68.35838 8 GAGCACA 52355 0.0 68.330826 9 AAGAGCA 54205 0.0 65.91111 7 GCCGTCT 38880 0.0 45.469627 50-51 ATGCCGT 38730 0.0 45.41708 48-49 TGCCGTC 38915 0.0 45.257835 50-51 TCGTATG 39195 0.0 45.224964 44-45 CGTATGC 38965 0.0 45.187164 46-47 TATGCCG 38805 0.0 45.182407 48-49 GTATGCC 39150 0.0 45.10103 46-47 CGTATCT 38665 0.0 45.025604 38-39 TATCTCG 38820 0.0 44.949394 40-41 TCTCGTA 39065 0.0 44.88154 42-43 TCGTATC 38730 0.0 44.79675 38-39 >>END_MODULE