FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221056
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3282836.287674370855245TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56620.10844549455129385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG440650.081.436213
GATCGGA442200.081.03971
ATCGGAA442500.080.634242
CGGAAGA443300.080.5851444
GAAGAGC461250.077.6424946
GGAAGAG497700.072.003915
AGAGCAC523200.068.358388
GAGCACA523550.068.3308269
AAGAGCA542050.065.911117
GCCGTCT388800.045.46962750-51
ATGCCGT387300.045.4170848-49
TGCCGTC389150.045.25783550-51
TCGTATG391950.045.22496444-45
CGTATGC389650.045.18716446-47
TATGCCG388050.045.18240748-49
GTATGCC391500.045.1010346-47
CGTATCT386650.045.02560438-39
TATCTCG388200.044.94939440-41
TCTCGTA390650.044.8815442-43
TCGTATC387300.044.7967538-39
Produced by FastQC (version 0.11.2)