##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34211-2.34100000035863.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3489055 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65252023828802 33.0 33.0 33.0 33.0 33.0 2 32.668879682320856 33.0 33.0 33.0 33.0 33.0 3 32.719749903627196 33.0 33.0 33.0 33.0 33.0 4 36.6549696121156 37.0 37.0 37.0 37.0 37.0 5 36.61649243133169 37.0 37.0 37.0 37.0 37.0 6 36.52020618763533 37.0 37.0 37.0 37.0 37.0 7 36.45597045618369 37.0 37.0 37.0 37.0 37.0 8 36.42244160668147 37.0 37.0 37.0 37.0 37.0 9 36.406825917046305 37.0 37.0 37.0 37.0 37.0 10-11 36.37495367656858 37.0 37.0 37.0 37.0 37.0 12-13 36.3560370931384 37.0 37.0 37.0 37.0 37.0 14-15 38.20077943741214 40.0 37.0 40.0 37.0 40.0 16-17 38.189035999719124 40.0 37.0 40.0 37.0 40.0 18-19 38.16976717191331 40.0 37.0 40.0 37.0 40.0 20-21 38.11357401932615 40.0 37.0 40.0 37.0 40.0 22-23 37.99540534614674 40.0 37.0 40.0 33.0 40.0 24-25 37.966645982938076 40.0 37.0 40.0 33.0 40.0 26-27 37.90457258484031 40.0 37.0 40.0 33.0 40.0 28-29 37.73394242853724 40.0 37.0 40.0 33.0 40.0 30-31 37.68286441457644 40.0 37.0 40.0 33.0 40.0 32-33 37.52916104790552 40.0 37.0 40.0 33.0 40.0 34-35 37.435694192266965 40.0 37.0 40.0 33.0 40.0 36-37 37.39781645746484 40.0 37.0 40.0 33.0 40.0 38-39 37.249660294836275 40.0 37.0 40.0 33.0 40.0 40-41 37.12423507224736 40.0 37.0 40.0 33.0 40.0 42-43 36.98903299030826 40.0 37.0 40.0 33.0 40.0 44-45 36.85686052527117 40.0 37.0 40.0 33.0 40.0 46-47 36.84071145338781 37.0 37.0 40.0 33.0 40.0 48-49 36.63009382196612 37.0 37.0 40.0 33.0 40.0 50-51 36.52426975212486 37.0 37.0 40.0 33.0 40.0 52-53 36.41745028381611 37.0 37.0 40.0 33.0 40.0 54-55 36.11053193486488 37.0 37.0 40.0 33.0 40.0 56-57 35.991690156790305 37.0 37.0 40.0 27.0 40.0 58-59 35.81757610585101 37.0 37.0 40.0 27.0 40.0 60-61 35.579716140903486 37.0 35.0 40.0 27.0 40.0 62-63 35.324731195123036 37.0 33.0 40.0 27.0 40.0 64-65 35.117791350379974 37.0 33.0 40.0 27.0 40.0 66-67 34.740930280548746 37.0 33.0 40.0 27.0 40.0 68-69 34.52302041670309 37.0 33.0 37.0 27.0 40.0 70-71 34.11499618091432 37.0 33.0 37.0 27.0 40.0 72-73 33.76120109886488 37.0 33.0 37.0 27.0 40.0 74-75 33.30109671529971 37.0 33.0 37.0 24.5 40.0 76-77 30.732528578655252 33.0 30.0 35.0 22.0 37.0 78-79 32.824354158934156 37.0 33.0 37.0 22.0 37.0 80-81 33.08225393408817 37.0 33.0 37.0 24.5 37.0 82-83 32.97777378115278 37.0 33.0 37.0 24.5 37.0 84-85 32.790381922898895 37.0 33.0 37.0 22.0 37.0 86-87 32.607136602891046 37.0 33.0 37.0 22.0 37.0 88-89 32.343201812525166 37.0 33.0 37.0 22.0 37.0 90-91 32.050827086417385 33.0 33.0 37.0 22.0 37.0 92-93 32.01993261785785 33.0 33.0 37.0 22.0 37.0 94-95 31.920511857795304 33.0 33.0 37.0 22.0 37.0 96-97 31.742260296842556 33.0 33.0 37.0 22.0 37.0 98-99 31.567892308948984 33.0 33.0 37.0 15.0 37.0 100-101 29.88744029543816 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.13467861782439883 2306 2 0.19269060884367661 2306 3 0.12820343010749724 2306 4 0.1692932120264956 2306 5 0.22949746803418947 2306 6 0.20991756167654074 2306 7 0.21197033823111866 2306 8 0.22893404917840599 2306 9 0.20650567333854042 2306 10-11 0.21847990530853423 2306 12-13 0.25217276908695396 2306 14-15 0.42752985593694603 2306 16-17 0.4236735007221384 2306 18-19 0.4174392579404298 2306 20-21 0.3689253370759147 2306 22-23 0.38021719609825766 2306 24-25 0.3755772656324865 2306 26-27 0.4427055707171661 2306 28-29 0.49239060957017955 2306 30-31 0.4067406093286152 2306 32-33 0.45153143872508394 2306 34-35 0.4368485755578533 2306 36-37 0.43223931476860145 2306 38-39 0.3199678519188325 2306 40-41 0.3400689690993701 2306 42-43 0.28603465639515235 2306 44-45 0.34663242106505265 2306 46-47 0.5206959317773467 2306 48-49 0.6008927613599155 2306 50-51 0.3603300577694526 2306 52-53 0.24507230983314798 2306 54-55 0.4781823437805741 2306 56-57 0.51909323050333 2306 58-59 0.4396316483513445 2306 60-61 0.5245095796610997 2306 62-63 0.4135771834241808 2306 64-65 0.42931998446927366 2306 66-67 0.5511711586088168 2306 68-69 0.5112308912348524 2306 70-71 0.5873817943468964 2306 72-73 0.426813168539141 2306 74-75 0.5126031552600949 2306 76-77 0.11854437280154784 2306 78-79 0.6018099410171445 2306 80-81 0.5600143384933247 2306 82-83 0.5254859398562743 2306 84-85 0.5762500766361285 2306 86-87 0.48704043183565915 2306 88-89 0.7493723115023059 2306 90-91 0.7350346365833644 2306 92-93 0.5819516231768489 2306 94-95 0.5137398695446009 2306 96-97 0.5108318355969637 2306 98-99 0.35613220918406796 2306 100-101 0.40096510634769444 2307 1 0.1498273861004833 2307 2 0.12732895772182928 2307 3 0.15114633255596743 2307 4 0.1201526094619112 2307 5 0.17748862808962684 2307 6 0.19286995333103363 2307 7 0.1288588775176862 2307 8 0.12231183195050477 2307 9 0.20166867542366873 2307 10-11 0.22947414935593002 2307 12-13 0.21857698429215588 2307 14-15 0.3249565330916795 2307 16-17 0.2712508814515573 2307 18-19 0.2893464634941125 2307 20-21 0.27497145961152825 2307 22-23 0.2764337497684579 2307 24-25 0.30340036685485217 2307 26-27 0.34549814416487123 2307 28-29 0.34669358993001254 2307 30-31 0.3534774461766901 2307 32-33 0.30012391478356193 2307 34-35 0.2972019236244279 2307 36-37 0.2610582831920212 2307 38-39 0.29461718829869454 2307 40-41 0.15750988006313804 2307 42-43 0.28930393758779616 2307 44-45 0.18910585753107512 2307 46-47 0.21730678692961192 2307 48-49 0.23781800889084792 2307 50-51 0.19006248713105123 2307 52-53 0.012531325203163135 2307 54-55 0.19393767209461998 2307 56-57 0.24166205846152877 2307 58-59 0.38356242709053134 2307 60-61 0.19987834768746637 2307 62-63 0.15185314451843368 2307 64-65 0.09744249511341252 2307 66-67 0.170328616999889 2307 68-69 0.1355407387832699 2307 70-71 0.12105374250587175 2307 72-73 -0.05480848682788064 2307 74-75 -0.11258918844893628 2307 76-77 -0.2320323461806062 2307 78-79 0.041128466194756186 2307 80-81 0.15759384180829983 2307 82-83 -0.00272821654082378 2307 84-85 0.10577649034054559 2307 86-87 -0.03677472841955165 2307 88-89 0.18482498408724268 2307 90-91 0.15427824697654557 2307 92-93 0.029633385172580518 2307 94-95 0.2082446203905235 2307 96-97 -0.034371745329732306 2307 98-99 -0.09662028485396235 2307 100-101 -0.07174293825157108 2201 1 0.0950322650789488 2201 2 0.17861254834504336 2201 3 0.05726601999640479 2201 4 0.09538655266755569 2201 5 0.1547098973863683 2201 6 0.13789711170725383 2201 7 0.13368973324476485 2201 8 0.1101567909747061 2201 9 0.17211369162340162 2201 10-11 0.1914240731218868 2201 12-13 0.20247659359270642 2201 14-15 0.36599069547018104 2201 16-17 0.26072653420551717 2201 18-19 0.3403668084531333 2201 20-21 0.2651911089349497 2201 22-23 0.3227118610701609 2201 24-25 0.3143332809009678 2201 26-27 0.3195985825105865 2201 28-29 0.4321286004121987 2201 30-31 0.39286662094323077 2201 32-33 0.42229324964157655 2201 34-35 0.4230319217185752 2201 36-37 0.4148761791325555 2201 38-39 0.3037047621504243 2201 40-41 0.39482977714718714 2201 42-43 0.40468558424302614 2201 44-45 0.48537181903287774 2201 46-47 0.5130028989936477 2201 48-49 0.6406605856013528 2201 50-51 0.5539823460980813 2201 52-53 0.5345520036861018 2201 54-55 0.5670994788413282 2201 56-57 0.5583880447394023 2201 58-59 0.5884602258326694 2201 60-61 0.567354762781811 2201 62-63 0.5420556411831967 2201 64-65 0.6352831659729503 2201 66-67 0.5395672295403955 2201 68-69 0.372608348846164 2201 70-71 0.5038196087151263 2201 72-73 0.48234750707339913 2201 74-75 0.6491575239581522 2201 76-77 0.4812011682085675 2201 78-79 0.7488647917141051 2201 80-81 0.6441304341467742 2201 82-83 0.6579576515742858 2201 84-85 0.6397828272949937 2201 86-87 0.6053762626229471 2201 88-89 0.7985092322290441 2201 90-91 0.7876213477960619 2201 92-93 0.697775180484193 2201 94-95 0.6505954875526996 2201 96-97 0.5925615022403043 2201 98-99 0.386081735348057 2201 100-101 0.4927584434903771 1101 1 0.1290828622098914 1101 2 0.12847298223663728 1101 3 0.1088560136364336 1101 4 0.09301834547371612 1101 5 0.1301886621379751 1101 6 0.16526355224149114 1101 7 0.14395137553152892 1101 8 0.15271473740958896 1101 9 0.09047811816569151 1101 10-11 0.10449514583759623 1101 12-13 0.12251183845172875 1101 14-15 0.22997866451830618 1101 16-17 0.2540825691147077 1101 18-19 0.251465405725412 1101 20-21 0.23526654529987212 1101 22-23 0.23108361924978738 1101 24-25 0.20685854413324734 1101 26-27 0.20123415596830085 1101 28-29 0.23398447632202135 1101 30-31 0.1746611937649405 1101 32-33 0.16712312032209553 1101 34-35 0.15649598786231422 1101 36-37 0.19099359132957971 1101 38-39 0.22438792941775176 1101 40-41 0.2869548919800664 1101 42-43 0.3041388030968193 1101 44-45 0.2880968914659263 1101 46-47 0.3230030756596065 1101 48-49 0.25932181095645035 1101 50-51 0.16651237931127838 1101 52-53 0.22410662880093923 1101 54-55 0.42743443750149623 1101 56-57 0.22020478913908903 1101 58-59 0.3426635506860336 1101 60-61 0.31253752371743104 1101 62-63 0.28891363708454776 1101 64-65 0.17278581596235654 1101 66-67 0.1496283559625553 1101 68-69 0.06606508335256223 1101 70-71 0.2777816962001154 1101 72-73 0.15344937266593206 1101 74-75 0.1781360413774138 1101 76-77 0.07315889204433645 1101 78-79 0.33551502844648695 1101 80-81 0.46594249689853484 1101 82-83 0.4440315156121599 1101 84-85 0.45320016529230855 1101 86-87 0.4623717929741602 1101 88-89 0.4342359476555089 1101 90-91 0.3911877918636151 1101 92-93 0.43848255098803435 1101 94-95 0.3501944558235053 1101 96-97 0.3979060891952919 1101 98-99 0.21777649190236303 1101 100-101 0.3345174181211483 2304 1 0.12722442236571396 2304 2 0.1258472350509976 2304 3 0.13593418240112243 2304 4 0.13924163727587313 2304 5 0.1540476278291436 2304 6 0.14644466896879038 2304 7 0.11731883526130815 2304 8 0.09566007078584704 2304 9 0.10854257198237605 2304 10-11 0.17466741438443734 2304 12-13 0.21837387873804914 2304 14-15 0.33546491200980455 2304 16-17 0.30823367500312315 2304 18-19 0.28632932940247713 2304 20-21 0.23511979106358183 2304 22-23 0.3007910766186228 2304 24-25 0.2951927896501374 2304 26-27 0.2716606286081529 2304 28-29 0.33271157783195804 2304 30-31 0.2710619589259622 2304 32-33 0.27671446209044603 2304 34-35 0.3847182762625536 2304 36-37 0.3319888640724571 2304 38-39 0.34562034305543676 2304 40-41 0.33622004764151114 2304 42-43 0.3919875791611247 2304 44-45 0.4752733345939646 2304 46-47 0.36355298819730564 2304 48-49 0.3235292039358555 2304 50-51 0.3880061067079126 2304 52-53 0.36654502958219837 2304 54-55 0.5146889528100829 2304 56-57 0.487896320856251 2304 58-59 0.5404439934706673 2304 60-61 0.5945106303999808 2304 62-63 0.42998228694557383 2304 64-65 0.3350340264728544 2304 66-67 0.42003658493135987 2304 68-69 0.5056935563572935 2304 70-71 0.4014629202431266 2304 72-73 0.2475661911843332 2304 74-75 0.4686372594107553 2304 76-77 0.34947766800132385 2304 78-79 0.36505367820019785 2304 80-81 0.5254952050105288 2304 82-83 0.47962974704922345 2304 84-85 0.4921513420090946 2304 86-87 0.4915509350923557 2304 88-89 0.5140650205550727 2304 90-91 0.7257909383602978 2304 92-93 0.5375577578390747 2304 94-95 0.5698729379500662 2304 96-97 0.6005689944462524 2304 98-99 0.3916333112792927 2304 100-101 0.41052269805418007 2202 1 0.116228141679656 2202 2 0.1478500156715583 2202 3 0.08768073814502486 2202 4 0.05244118792443686 2202 5 0.12330443483358522 2202 6 0.11901472868859742 2202 7 0.10732514903311596 2202 8 0.13361940753446078 2202 9 0.11575071270955561 2202 10-11 0.10670454891702263 2202 12-13 0.062315920578356554 2202 14-15 0.1433769003948271 2202 16-17 0.14373363264864736 2202 18-19 0.06263305925578777 2202 20-21 0.10578165854135335 2202 22-23 0.16397405484743643 2202 24-25 0.15497288127370012 2202 26-27 0.07565790610187406 2202 28-29 0.09152075466779763 2202 30-31 0.1375380591819635 2202 32-33 0.21125266533480414 2202 34-35 0.12602101866559678 2202 36-37 0.10478277458238239 2202 38-39 0.09488997460105253 2202 40-41 0.06336402133839414 2202 42-43 0.23005958620181133 2202 44-45 0.19593405702342181 2202 46-47 0.29834661109078553 2202 48-49 0.25756869554425066 2202 50-51 0.17587593269011137 2202 52-53 0.15331850258023394 2202 54-55 0.31975389551826083 2202 56-57 0.3091806267501198 2202 58-59 0.2684690387554767 2202 60-61 0.321842367396421 2202 62-63 0.14721494401911173 2202 64-65 0.20113983287744475 2202 66-67 0.10216870922500476 2202 68-69 0.18152446744145578 2202 70-71 0.39464625060507785 2202 72-73 0.20313554614536855 2202 74-75 0.3471310111406325 2202 76-77 0.065902450392894 2202 78-79 0.5348054908239988 2202 80-81 0.5334120444604693 2202 82-83 0.37422349375567165 2202 84-85 0.379902070653813 2202 86-87 0.4589000669733494 2202 88-89 0.5444999739306766 2202 90-91 0.5822234258189383 2202 92-93 0.45001881388389364 2202 94-95 0.4678080095242052 2202 96-97 0.27207845623906834 2202 98-99 0.4172565376856312 2202 100-101 0.2453126641804495 1102 1 0.1441310027598206 1102 2 0.15845752316021589 1102 3 0.09234488575586397 1102 4 0.09787120616127254 1102 5 0.15129036465882706 1102 6 0.13696674303025702 1102 7 0.12613627518657466 1102 8 0.1124063648597513 1102 9 0.04928353478337755 1102 10-11 0.08053528381901742 1102 12-13 0.11520454917486944 1102 14-15 0.2016764831832134 1102 16-17 0.1414437313136645 1102 18-19 0.12882481092385945 1102 20-21 0.12714326200944726 1102 22-23 0.10628930839215656 1102 24-25 0.09513322019417814 1102 26-27 0.17392272067584713 1102 28-29 0.23669771487845281 1102 30-31 0.2511627023837164 1102 32-33 0.12868001237780646 1102 34-35 0.1787820856905853 1102 36-37 0.16029676998365971 1102 38-39 0.1478577273374171 1102 40-41 0.31355524767786136 1102 42-43 0.21128657189577638 1102 44-45 0.22629591538262162 1102 46-47 0.33979791488142297 1102 48-49 0.3144504828031103 1102 50-51 0.14387601900735802 1102 52-53 0.1255926846634452 1102 54-55 0.20673039854916908 1102 56-57 0.2872603975731991 1102 58-59 0.209871348229008 1102 60-61 0.2130586829332799 1102 62-63 0.16462405306389627 1102 64-65 0.1311095742749231 1102 66-67 0.22527879375905258 1102 68-69 0.15996418349021013 1102 70-71 0.0794591260749371 1102 72-73 0.05360408983308673 1102 74-75 0.0460984643190443 1102 76-77 -0.14719892932559375 1102 78-79 0.24667969225897224 1102 80-81 0.3086430446023556 1102 82-83 0.09787245776892206 1102 84-85 0.1871296779711571 1102 86-87 0.21616356439724171 1102 88-89 0.270293643001736 1102 90-91 0.4168363968178568 1102 92-93 0.2169402711092303 1102 94-95 0.28675991167664705 1102 96-97 0.1488178966179241 1102 98-99 0.05375385819652223 1102 100-101 0.16756925216423113 2305 1 0.13565578348325147 2305 2 0.1260161633336523 2305 3 0.09641476066628485 2305 4 0.09449523518546243 2305 5 0.12028443135240963 2305 6 -0.0014046135519336644 2305 7 0.04222388179663028 2305 8 0.08075065648672819 2305 9 0.05959685650061175 2305 10-11 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-1.1178691199078656 1213 50-51 -1.1163823258051835 1213 52-53 -1.030104041337779 1213 54-55 -0.9642171594829634 1213 56-57 -1.0002309055129786 1213 58-59 -1.1130970865003178 1213 60-61 -1.2274320958032305 1213 62-63 -1.0132119918235247 1213 64-65 -1.0592754352633165 1213 66-67 -1.1500871284373133 1213 68-69 -1.3931073298862557 1213 70-71 -1.2170467907234226 1213 72-73 -1.0292998597849206 1213 74-75 -1.1462661945615622 1213 76-77 -1.0396127640936434 1213 78-79 -1.1549398348413575 1213 80-81 -1.1518365132443762 1213 82-83 -1.2500772432889988 1213 84-85 -1.507427979248046 1213 86-87 -1.602382631484538 1213 88-89 -1.6122451810837788 1213 90-91 -1.3666008226900779 1213 92-93 -1.4642858500985874 1213 94-95 -1.4159787289830454 1213 96-97 -1.6482145727455197 1213 98-99 -1.5693903676562329 1213 100-101 -1.6290252029587258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 36.0 9 146.0 10 203.0 11 144.0 12 137.0 13 240.0 14 537.0 15 1202.0 16 2207.0 17 3511.0 18 5094.0 19 7066.0 20 9162.0 21 11840.0 22 14748.0 23 18744.0 24 23209.0 25 28540.0 26 35424.0 27 44545.0 28 58526.0 29 77910.0 30 84148.0 31 88510.0 32 114174.0 33 157061.0 34 229897.0 35 364528.0 36 622276.0 37 1014845.0 38 468760.0 39 1680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.96292102053333 29.21482814621182 16.059594662574856 17.762656170679996 2 31.00524354015629 27.04121889738052 27.451989148924277 14.50154841353891 3 35.141320500823284 22.644555617495282 24.1078458207165 18.106278060964932 4 29.97070873174455 23.661749628018953 26.59351349894934 19.774028141287157 5 34.40212321101272 21.413964526211252 26.268144239629354 17.91576802314667 6 31.61563804525867 22.04745984227821 28.213312773802652 18.123589338660466 7 32.367388877504084 24.30125062516928 23.029444935663097 20.301915561663545 8 29.17297090472922 28.51098649920967 22.784765502406813 19.531277093654296 9 30.746319562173714 23.235317299383357 25.09097735633288 20.927385782110054 10-11 25.701999279732497 29.97870582002226 27.844515655929964 16.474779244315283 12-13 24.536342935264706 29.45456577783956 27.390812125346258 18.618279161549474 14-15 23.955798919764806 29.574512296309457 27.519414282663927 18.950274501261802 16-17 24.936934721507484 28.108806897936354 27.608985098638065 19.345273281918097 18-19 23.020044109364857 28.13965386042926 29.163297798401 19.677004231804887 20-21 24.08213685367528 28.299066652718285 29.052150224057804 18.56664626954863 22-23 22.6677854032109 31.329686691668662 27.437042408331198 18.565485496789243 24-25 22.265541815763868 28.569483714071573 29.049714034315883 20.115260435848676 26-27 22.263134287077733 28.456315535295374 27.724469806294255 21.55608037133264 28-29 23.39023030591378 29.928146733141208 27.634459760594197 19.047163200350813 30-31 21.91931626185314 28.514096223762596 29.114760300425186 20.45182721395908 32-33 21.879047478471964 29.891073657480323 27.700380188905015 20.529498675142698 34-35 21.685144213138546 29.8427267329874 29.216683201709802 19.255445852164247 36-37 21.739511128371436 28.426694334139185 29.139881715822767 20.69391282166661 38-39 22.9063045780852 29.886567150489952 27.791098287880946 19.416029983543904 40-41 21.472888258816187 31.09090158645899 27.8386878844223 19.597522270302527 42-43 21.45193285225438 29.774133460286052 29.06921053619657 19.704723151263 44-45 21.31212405134183 28.410246812345175 29.221208211964033 21.056420924348963 46-47 22.72214465697418 28.33663769888894 27.75002085232854 21.191196791808345 48-49 21.29438659445199 29.78287872397234 29.02875788835847 19.893976793217195 50-51 22.534546374877074 28.366435349530978 29.058838973521734 20.040179302070218 52-53 21.262375577808818 28.409233246589753 27.74072775749557 22.587663418105855 54-55 22.35437347549483 28.42015900003067 29.231025903652274 19.99444162082222 56-57 21.125784870795712 28.334828325325162 29.188464452587827 21.3509223512913 58-59 21.021081587046297 28.560798597523302 29.089049529617 21.329070285813398 60-61 22.28996365441941 28.397127787483566 29.12938602400907 20.18352253408796 62-63 20.93769451161756 28.502620814511285 29.0351965793616 21.524488094509554 64-65 22.160492668154312 28.46595592479263 29.090075446153367 20.28347596089969 66-67 20.959235769400074 30.869057739711113 27.684252335651532 20.487454155237277 68-69 20.869781852156695 31.06335498671354 27.611801020544192 20.455062140585568 70-71 20.831012327444025 30.92964564081408 27.68451387600768 20.554828155734214 72-73 20.7658596122326 30.95518522261186 27.652633336701733 20.62632182845381 74-75 20.604862715146165 30.827419040160713 27.86047443074346 20.70724381394966 76-77 20.743060505974594 30.579959837279198 27.803631846408628 20.873347810337577 78-79 20.67816317500515 30.585598944137953 27.79224584110268 20.943992039754214 80-81 20.71375042081806 30.38046251935333 27.80552179217686 21.10026526765175 82-83 20.754967904806996 30.376632704651996 27.68313577888883 21.18526361165218 84-85 20.63797486205459 30.26657240283485 27.86380490998382 21.231647825126736 86-87 20.81968707642133 30.00966705426623 27.786187235818787 21.384458633493647 88-89 20.78384862790225 30.178123438485017 27.68626445910913 21.351763474503606 90-91 20.73577890627049 29.832795373030162 27.762736357693175 21.668689363006173 92-93 20.80186031708256 29.910001999955554 27.714997014403263 21.57314066855862 94-95 20.602500029748946 29.87999920287161 27.851895265931926 21.66560550144752 96-97 20.74406969784936 29.717517282902023 27.792468991122917 21.745944028125706 98-99 20.640595771816162 29.819858334164838 27.77344820744319 21.766097686575808 100-101 20.732000686996134 29.711292455916556 27.68323457047115 21.873472286616163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 7.0 2 11.5 3 25.5 4 24.0 5 10.5 6 14.0 7 14.5 8 15.5 9 22.5 10 24.5 11 31.5 12 44.0 13 62.5 14 89.0 15 126.0 16 188.0 17 317.5 18 536.0 19 875.5 20 1463.0 21 2315.0 22 3533.0 23 5394.0 24 7922.5 25 11200.5 26 15234.0 27 20216.5 28 26387.5 29 33301.0 30 41148.0 31 50010.5 32 60127.5 33 71624.0 34 84109.5 35 98725.5 36 114446.0 37 130312.0 38 144829.5 39 156859.5 40 166744.0 41 172686.5 42 174562.5 43 172757.5 44 168957.0 45 165203.5 46 160853.5 47 155372.5 48 147659.5 49 137950.0 50 127220.0 51 115091.5 52 100945.0 53 85457.0 54 71051.5 55 58488.0 56 47601.5 57 38617.5 58 31135.0 59 24870.5 60 19935.5 61 15648.0 62 12034.5 63 9030.0 64 6744.0 65 5118.0 66 3813.5 67 2876.5 68 2127.0 69 1594.5 70 1237.0 71 972.0 72 744.5 73 575.5 74 430.5 75 323.5 76 241.5 77 187.5 78 152.5 79 104.0 80 74.5 81 64.5 82 50.5 83 41.5 84 27.5 85 22.0 86 19.0 87 13.5 88 7.0 89 3.5 90 4.5 91 4.0 92 2.0 93 1.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18133849996632326 2 0.0 3 0.0 4 0.008655638847768236 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021065876003674346 12-13 0.0 14-15 0.0 16-17 3.2960214155408846E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.4330527893656017E-5 36-37 0.0 38-39 0.0021352486561547466 40-41 0.0061907880500594 42-43 0.0026368171324327077 44-45 0.0013757306777909778 46-47 0.006764009165805641 48-49 0.008326036706214146 50-51 0.005560244822738535 52-53 0.006534720719507144 54-55 0.01799914303443196 56-57 0.02126650339418553 58-59 0.026941392440073313 60-61 0.025021101702323408 62-63 0.010017038997665557 64-65 0.015491300653042156 66-67 0.02259924248829554 68-69 0.02625352710117783 70-71 0.028202478894715048 72-73 0.021567444479952305 74-75 0.03171345822866077 76-77 0.028503419980481823 78-79 0.020650290694758322 80-81 0.009013902045109635 82-83 0.0016910022914514104 84-85 0.007609510311531346 86-87 0.02633951026853976 88-89 0.0245338637539391 90-91 0.020764934917907568 92-93 0.04234670992575354 94-95 0.04403771221720494 96-97 0.032745256237004 98-99 0.040440749715897284 100-101 0.04035476654853535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3489055.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.43497664065504 #Duplication Level Percentage of deduplicated Percentage of total 1 97.73201089768617 91.3158815526955 2 1.7344023434797473 3.2410768489705513 3 0.22278760867309585 0.6244846503659433 4 0.09512355133954568 0.3555146718954641 5 0.07093352613457792 0.3313836178711791 6 0.03068612971833043 0.17202946880545883 7 0.028214080013132324 0.18453273348752136 8 0.016477443549045685 0.12316556424822364 9 0.008798233966633243 0.07398565066362554 >10 0.05690715120370795 1.0150588860265874 >50 0.002578878440030345 0.15840473957497167 >100 8.629430818715763E-4 0.14313340177859998 >500 1.5466263671549008E-4 0.10113262537546842 >1k 3.195716537339546E-5 0.040190442822217606 >5k 0.0 0.0 >10k+ 3.059291205544462E-5 2.120025145418722 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 74167 2.125704524577572 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0035826319734140045 0.0 0.0 2 0.00449978575860799 0.0 0.0 3 0.007021958667891449 0.0 0.0 4 0.00942948735402566 0.0 2.8661055787312038E-5 5 0.012180948709607616 0.0 5.7322111574624076E-5 6 0.021725080286782526 0.0 5.7322111574624076E-5 7 0.031068584473446247 0.0 5.7322111574624076E-5 8 0.039437612763341366 0.0 5.7322111574624076E-5 9 0.043937398521949354 0.0 5.7322111574624076E-5 10-11 0.053266572180719424 0.0 8.598316736193611E-5 12-13 0.06475965555143154 1.4330527893656019E-5 1.2897475104290418E-4 14-15 0.07642470525686755 2.8661055787312038E-5 2.0062739051118425E-4 16-17 0.08965178250271205 2.8661055787312038E-5 2.5794950208580836E-4 18-19 0.10683408544720563 2.8661055787312038E-5 2.5794950208580836E-4 20-21 0.12751303719775126 2.8661055787312038E-5 2.866105578731204E-4 22-23 0.14902315956612894 2.8661055787312038E-5 2.866105578731204E-4 24-25 0.17007470504190964 2.8661055787312038E-5 3.152716136604324E-4 26-27 0.1907393262645616 2.8661055787312038E-5 3.2960214155408846E-4 28-29 0.21292298344394112 2.8661055787312038E-5 3.4393266944774444E-4 30-31 0.24294543938115049 2.8661055787312038E-5 3.5826319734140043E-4 32-33 0.27709508735173277 2.8661055787312038E-5 3.7259372523505647E-4 34-35 0.3143401293473448 2.8661055787312038E-5 4.012547810223685E-4 36-37 0.35920901218238177 2.8661055787312038E-5 4.4424636470333657E-4 38-39 0.4129628223114855 2.8661055787312038E-5 4.8723794838430464E-4 40-41 0.47352363319007584 2.8661055787312038E-5 5.445600599589288E-4 42-43 0.5395587057240427 2.8661055787312038E-5 5.875516436398968E-4 44-45 0.6106381240765766 2.8661055787312038E-5 6.018821715335528E-4 46-47 0.6860167007972073 2.8661055787312038E-5 6.018821715335528E-4 48-49 0.7669268612847892 2.8661055787312038E-5 6.018821715335528E-4 50-51 0.8551312604702419 2.8661055787312038E-5 6.018821715335528E-4 52-53 0.9465313673759801 2.8661055787312038E-5 6.305432273208648E-4 54-55 1.0425459042634753 2.8661055787312038E-5 6.305432273208648E-4 56-57 1.1429312521585357 2.8661055787312038E-5 6.305432273208648E-4 58-59 1.2478880384516724 2.8661055787312038E-5 6.878653388954889E-4 60-61 1.3570579999455439 2.8661055787312038E-5 7.16526394682801E-4 62-63 1.464923883401093 2.8661055787312038E-5 7.16526394682801E-4 64-65 1.5774901800057608 2.8661055787312038E-5 7.16526394682801E-4 66-67 1.6959749846305088 2.8661055787312038E-5 7.451874504701129E-4 68-69 1.8185583202328424 2.8661055787312038E-5 7.451874504701129E-4 70-71 1.945684433177465 2.8661055787312038E-5 7.451874504701129E-4 72-73 2.078155833026421 2.8661055787312038E-5 7.73848506257425E-4 74-75 2.21726226729014 2.8661055787312038E-5 8.311706178320491E-4 76-77 2.3622728790460457 2.8661055787312038E-5 8.598316736193612E-4 78-79 2.511109741749557 2.8661055787312038E-5 8.884927294066731E-4 80-81 2.6702645845365005 2.8661055787312038E-5 8.884927294066731E-4 82-83 2.830135953718127 2.8661055787312038E-5 9.171537851939852E-4 84-85 2.9977744690181156 2.8661055787312038E-5 9.458148409812972E-4 86-87 3.174054292637978 2.8661055787312038E-5 0.0010031369525559213 88-89 3.362959311332151 2.8661055787312038E-5 0.0010317980083432334 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCGC 25 0.0050260103 56.98888 3 CGGAAGA 22480 0.0 38.343277 4 TCGGAAG 22585 0.0 38.291176 3 GCCGTCT 11365 0.0 37.846382 50-51 AGAGCAC 22735 0.0 37.808224 8 GAGCACA 22690 0.0 37.799488 9 CGTCTTC 11440 0.0 37.497147 52-53 ATCGGAA 23225 0.0 37.09287 2 GAAGAGC 23340 0.0 36.929928 6 ATGCCGT 11600 0.0 36.85339 48-49 TGCCGTC 11470 0.0 36.83731 50-51 GATCGGA 23515 0.0 36.820446 1 CCGTCTT 11535 0.0 36.611767 52-53 CGGGCCG 105 4.5994966E-8 36.246273 1 CTGCTTG 12015 0.0 35.923973 58-59 TATGCCG 11770 0.0 35.675392 48-49 CGTATGC 12125 0.0 34.76674 46-47 TGCTTGA 11955 0.0 34.690742 60-61 TCGTATG 12415 0.0 34.623165 44-45 GCTTGAA 12025 0.0 34.58757 60-61 >>END_MODULE