FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489055
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT741672.125704524577572TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC250.005026010356.988883
CGGAAGA224800.038.3432774
TCGGAAG225850.038.2911763
GCCGTCT113650.037.84638250-51
AGAGCAC227350.037.8082248
GAGCACA226900.037.7994889
CGTCTTC114400.037.49714752-53
ATCGGAA232250.037.092872
GAAGAGC233400.036.9299286
ATGCCGT116000.036.8533948-49
TGCCGTC114700.036.8373150-51
GATCGGA235150.036.8204461
CCGTCTT115350.036.61176752-53
CGGGCCG1054.5994966E-836.2462731
CTGCTTG120150.035.92397358-59
TATGCCG117700.035.67539248-49
CGTATGC121250.034.7667446-47
TGCTTGA119550.034.69074260-61
TCGTATG124150.034.62316544-45
GCTTGAA120250.034.5875760-61
Produced by FastQC (version 0.11.2)