##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3800862 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.650990748940636 33.0 33.0 33.0 33.0 33.0 2 32.65985479083429 33.0 33.0 33.0 33.0 33.0 3 32.71104238985788 33.0 33.0 33.0 33.0 33.0 4 36.649375325912914 37.0 37.0 37.0 37.0 37.0 5 36.60391011302173 37.0 37.0 37.0 37.0 37.0 6 36.48820346542442 37.0 37.0 37.0 37.0 37.0 7 36.4422733579909 37.0 37.0 37.0 37.0 37.0 8 36.37641487641488 37.0 37.0 37.0 37.0 37.0 9 36.36075263979592 37.0 37.0 37.0 37.0 37.0 10-11 36.312953745755564 37.0 37.0 37.0 37.0 37.0 12-13 36.2908821735701 37.0 37.0 37.0 35.0 37.0 14-15 38.050371863014234 40.0 37.0 40.0 35.0 40.0 16-17 38.02476490859179 40.0 37.0 40.0 35.0 40.0 18-19 38.00656982547643 40.0 37.0 40.0 33.0 40.0 20-21 37.920733638842975 40.0 37.0 40.0 33.0 40.0 22-23 37.811929635961526 40.0 37.0 40.0 33.0 40.0 24-25 37.75324071223844 40.0 37.0 40.0 33.0 40.0 26-27 37.664484003891744 40.0 37.0 40.0 33.0 40.0 28-29 37.50118986166822 40.0 37.0 40.0 33.0 40.0 30-31 37.42248797772716 40.0 37.0 40.0 33.0 40.0 32-33 37.26738697695417 40.0 37.0 40.0 33.0 40.0 34-35 37.155589179507174 40.0 37.0 40.0 33.0 40.0 36-37 37.07577846814749 40.0 37.0 40.0 33.0 40.0 38-39 36.93742524722023 40.0 37.0 40.0 33.0 40.0 40-41 36.79715509271318 37.0 37.0 40.0 33.0 40.0 42-43 36.65604065604066 37.0 37.0 40.0 33.0 40.0 44-45 36.49687478787707 37.0 37.0 40.0 33.0 40.0 46-47 36.409857027169096 37.0 37.0 40.0 33.0 40.0 48-49 36.18548081987718 37.0 37.0 40.0 33.0 40.0 50-51 36.032558666955026 37.0 37.0 40.0 30.0 40.0 52-53 35.883760578521404 37.0 37.0 40.0 27.0 40.0 54-55 35.58268703257313 37.0 37.0 40.0 27.0 40.0 56-57 35.41159413312033 37.0 33.0 40.0 27.0 40.0 58-59 35.19864914853525 37.0 33.0 40.0 27.0 40.0 60-61 34.950432691321076 37.0 33.0 40.0 27.0 40.0 62-63 34.65808558690108 37.0 33.0 40.0 27.0 40.0 64-65 34.42984130968186 37.0 33.0 38.5 27.0 40.0 66-67 34.100075719665696 37.0 33.0 37.0 27.0 40.0 68-69 33.815963457763004 37.0 33.0 37.0 27.0 40.0 70-71 33.41357578886053 37.0 33.0 37.0 22.0 40.0 72-73 33.13972041079103 37.0 33.0 37.0 22.0 40.0 74-75 32.759462853426406 37.0 33.0 37.0 22.0 38.5 76-77 30.237604522342565 33.0 27.0 35.0 22.0 37.0 78-79 32.42844728380036 35.0 33.0 37.0 22.0 37.0 80-81 32.739738511948076 37.0 33.0 37.0 22.0 37.0 82-83 32.65267668228944 37.0 33.0 37.0 22.0 37.0 84-85 32.46421745909217 37.0 33.0 37.0 22.0 37.0 86-87 32.28105427137318 33.0 33.0 37.0 22.0 37.0 88-89 32.00963649824698 33.0 33.0 37.0 22.0 37.0 90-91 31.705649402688127 33.0 33.0 37.0 22.0 37.0 92-93 31.666589447341153 33.0 33.0 37.0 22.0 37.0 94-95 31.556428910073556 33.0 33.0 37.0 22.0 37.0 96-97 31.359871260782423 33.0 33.0 37.0 15.0 37.0 98-99 31.171146571488258 33.0 33.0 37.0 15.0 37.0 100-101 29.395654196337567 33.0 27.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.18157162863099785 2306 2 0.1838457482925122 2306 3 0.11117769731723826 2306 4 0.17309897353546688 2306 5 0.21663910910974948 2306 6 0.16966792559154698 2306 7 0.21954401898974396 2306 8 0.1289333132482966 2306 9 0.1543338418765714 2306 10-11 0.21998077772936853 2306 12-13 0.1709466130226076 2306 14-15 0.477168863244124 2306 16-17 0.44432322228929166 2306 18-19 0.3949642431886744 2306 20-21 0.3509077326120362 2306 22-23 0.37575590793074554 2306 24-25 0.40645526953642985 2306 26-27 0.34648014688801254 2306 28-29 0.3470759611636254 2306 30-31 0.4208535907201991 2306 32-33 0.4434852375454952 2306 34-35 0.3929370956797342 2306 36-37 0.5437506303906829 2306 38-39 0.3980100882426214 2306 40-41 0.3791842235934624 2306 42-43 0.2240491267048128 2306 44-45 0.4050651254050166 2306 46-47 0.4186221672374373 2306 48-49 0.4472960692006396 2306 50-51 0.3112289277293101 2306 52-53 0.20390286851949924 2306 54-55 0.3467449374314313 2306 56-57 0.40539072423622 2306 58-59 0.41845871240148824 2306 60-61 0.5415202056380366 2306 62-63 0.44860426716692103 2306 64-65 0.4564633036353527 2306 66-67 0.2724256198627302 2306 68-69 0.47118875656467907 2306 70-71 0.40216831715108725 2306 72-73 0.3893720063635868 2306 74-75 0.2671948460889979 2306 76-77 -0.06643453229942153 2306 78-79 0.46385283127096244 2306 80-81 0.45561510075963696 2306 82-83 0.414010092405789 2306 84-85 0.44447739550759735 2306 86-87 0.4654331826606395 2306 88-89 0.42499743813200297 2306 90-91 0.4414545079850214 2306 92-93 0.44678047593847126 2306 94-95 0.2982303186129478 2306 96-97 0.24799097600678977 2306 98-99 0.31570014568671567 2306 100-101 0.2769529499398935 2307 1 0.132234076401879 2307 2 0.1431439512051753 2307 3 0.13210747704219727 2307 4 0.11876608643149922 2307 5 0.1581676475057563 2307 6 0.10775562994430743 2307 7 0.08186157203018496 2307 8 0.22599022777509248 2307 9 0.18771626043970713 2307 10-11 0.18729184016091693 2307 12-13 0.19583383869296256 2307 14-15 0.3399131738197596 2307 16-17 0.3172740153605602 2307 18-19 0.31960218891095593 2307 20-21 0.2882224460110834 2307 22-23 0.33500969073873677 2307 24-25 0.325547192784974 2307 26-27 0.3416188836532541 2307 28-29 0.31689118164149477 2307 30-31 0.23979947526849088 2307 32-33 0.3110646460887594 2307 34-35 0.34542847533979426 2307 36-37 0.2450927666123377 2307 38-39 0.20873541378721683 2307 40-41 0.16168371651463787 2307 42-43 0.18309906382703645 2307 44-45 0.2165348426578646 2307 46-47 0.30130643968074367 2307 48-49 0.41310597549247063 2307 50-51 0.20047439444466164 2307 52-53 0.055219447968603674 2307 54-55 0.12820289045565403 2307 56-57 0.2402434685467938 2307 58-59 0.35122736276049693 2307 60-61 0.1291509305579197 2307 62-63 0.028961656319488327 2307 64-65 -0.005263401163638548 2307 66-67 0.14481781490552947 2307 68-69 0.12117739799909799 2307 70-71 -0.06451903833145423 2307 72-73 -0.0875692941921713 2307 74-75 -0.014641793398240566 2307 76-77 -0.33739250560679324 2307 78-79 0.04621196477467748 2307 80-81 0.21313974479030406 2307 82-83 0.18765680045009248 2307 84-85 0.34350846929770285 2307 86-87 0.2093224772125879 2307 88-89 0.32409686614709443 2307 90-91 0.44755020389999345 2307 92-93 0.16969638198690618 2307 94-95 0.1788643699698902 2307 96-97 0.021494484992224727 2307 98-99 0.052349774505017876 2307 100-101 0.16020007987377127 2201 1 0.1451603898059659 2201 2 0.18667780448664217 2201 3 0.12433382758543132 2201 4 0.10747956101950251 2201 5 0.2251688025963361 2201 6 0.13868931767328263 2201 7 0.1701921671378912 2201 8 0.08274876663271868 2201 9 0.12364099385613514 2201 10-11 0.21137445589028658 2201 12-13 0.16586679182209707 2201 14-15 0.27063390155830547 2201 16-17 0.32050697069286116 2201 18-19 0.27403512441471634 2201 20-21 0.3229192268649044 2201 22-23 0.33776660397160896 2201 24-25 0.31086778550066896 2201 26-27 0.26902325033628216 2201 28-29 0.26808250650206844 2201 30-31 0.27343421013271296 2201 32-33 0.3042251800742335 2201 34-35 0.3289580088342703 2201 36-37 0.2679667862016757 2201 38-39 0.09309868977518221 2201 40-41 0.23710727595616987 2201 42-43 0.2590004357724993 2201 44-45 0.3250539504369456 2201 46-47 0.37094097311227614 2201 48-49 0.4440465481278366 2201 50-51 0.3876273951622622 2201 52-53 0.30677563352588066 2201 54-55 0.4176189795770284 2201 56-57 0.4455799004814338 2201 58-59 0.4441244212137434 2201 60-61 0.375905582394104 2201 62-63 0.3469455826203003 2201 64-65 0.22865758843739314 2201 66-67 0.4012386786111364 2201 68-69 0.29906311799506824 2201 70-71 0.41448632481391456 2201 72-73 0.2748692924427729 2201 74-75 0.43924625094212644 2201 76-77 0.2210790373046656 2201 78-79 0.613783386826519 2201 80-81 0.44777027317342544 2201 82-83 0.3465508012180578 2201 84-85 0.25178263177835447 2201 86-87 0.2582149514984522 2201 88-89 0.5408858480936871 2201 90-91 0.5353976752902554 2201 92-93 0.3722713763216099 2201 94-95 0.20879305807654802 2201 96-97 0.2501884536568504 2201 98-99 0.16517332576334454 2201 100-101 0.5077080584967248 1101 1 0.13691983739209235 1101 2 0.15008004259363616 1101 3 0.1295199331773702 1101 4 0.11375182550096952 1101 5 0.1797841634719859 1101 6 0.07257103326791992 1101 7 0.10158351842780888 1101 8 0.12196976941124404 1101 9 0.17995935487678594 1101 10-11 0.19555357009213736 1101 12-13 0.18560478124879154 1101 14-15 0.2858770497804741 1101 16-17 0.34685015085440085 1101 18-19 0.23982350749900405 1101 20-21 0.28038892365646717 1101 22-23 0.31664773907316857 1101 24-25 0.2518661748160653 1101 26-27 0.3392162532637357 1101 28-29 0.2355420115986533 1101 30-31 0.3524902034665942 1101 32-33 0.28821759349504106 1101 34-35 0.3102189206345898 1101 36-37 0.3576143901613662 1101 38-39 0.33625799520174127 1101 40-41 0.3412909655212033 1101 42-43 0.21791383355281368 1101 44-45 0.4039367143481982 1101 46-47 0.3408740645489985 1101 48-49 0.40939251955484224 1101 50-51 0.26461870687088407 1101 52-53 0.3739411935816932 1101 54-55 0.5374991898085995 1101 56-57 0.3076669298656469 1101 58-59 0.3757818930659482 1101 60-61 0.4715322650892375 1101 62-63 0.4011429394907964 1101 64-65 0.327572078622417 1101 66-67 0.26607313031215085 1101 68-69 0.32877572983183967 1101 70-71 0.39222135348097 1101 72-73 0.3452346675245792 1101 74-75 0.3617730809894226 1101 76-77 0.1561313527127659 1101 78-79 0.3537094437648065 1101 80-81 0.4615544120450039 1101 82-83 0.3372117819917264 1101 84-85 0.28138581944466523 1101 86-87 0.2727624877584489 1101 88-89 0.4160721814740427 1101 90-91 0.5687652503673633 1101 92-93 0.4909597387334834 1101 94-95 0.3278158775779829 1101 96-97 0.3889544061945607 1101 98-99 0.3304196213847099 1101 100-101 0.46226977887247145 2304 1 0.11181212887149883 2304 2 0.1603921648389317 2304 3 0.1477505688901033 2304 4 0.13259151802801483 2304 5 0.18407778154842447 2304 6 0.10312150904513118 2304 7 0.115267924713649 2304 8 0.09510695442193651 2304 9 0.12669555423828172 2304 10-11 0.10449376474235805 2304 12-13 0.12114743072342549 2304 14-15 0.18190190797717065 2304 16-17 0.15666208712815433 2304 18-19 0.2035861768106031 2304 20-21 0.0864380356423382 2304 22-23 0.23859741827224923 2304 24-25 0.22910558218674026 2304 26-27 0.1717074196152808 2304 28-29 0.08636648545414971 2304 30-31 0.07840650077310585 2304 32-33 0.06215406871432805 2304 34-35 0.2916973169399526 2304 36-37 0.22924365588371387 2304 38-39 0.17933404206657144 2304 40-41 0.12327032267955929 2304 42-43 0.17556307571876317 2304 44-45 0.24130730914719578 2304 46-47 0.1384393870546532 2304 48-49 -0.018573339168774794 2304 50-51 0.027383328883708202 2304 52-53 -0.0035911189744908256 2304 54-55 0.11302920871570166 2304 56-57 0.24100039234588877 2304 58-59 0.15064125208402857 2304 60-61 0.06545719457502486 2304 62-63 0.10071946678211674 2304 64-65 -0.013961179289132986 2304 66-67 0.01903937647649201 2304 68-69 0.10968923756515636 2304 70-71 0.02492444990721765 2304 72-73 0.02948985148142924 2304 74-75 -0.007478073583925493 2304 76-77 -0.08800860637349572 2304 78-79 0.18103176344990146 2304 80-81 0.244965750110282 2304 82-83 0.20770778360348174 2304 84-85 0.2882171742473787 2304 86-87 0.23420903643650348 2304 88-89 0.2427102808448396 2304 90-91 0.43600597503648686 2304 92-93 0.1534182825762791 2304 94-95 0.09089626377889104 2304 96-97 0.08943638245219532 2304 98-99 -0.06155454406797389 2304 100-101 -0.16017427468733203 2202 1 0.07582147169439679 2202 2 0.11817092092277193 2202 3 0.06875459625006641 2202 4 0.03124084421970963 2202 5 0.11849723842550475 2202 6 0.058494039841384904 2202 7 0.0770895306030468 2202 8 0.12651648964503437 2202 9 0.06872806660977915 2202 10-11 0.06489917069860951 2202 12-13 0.05263838954489586 2202 14-15 0.1313935964519075 2202 16-17 0.1235322618373047 2202 18-19 0.11059512831108265 2202 20-21 0.15774075207216498 2202 22-23 0.08513757773614117 2202 24-25 0.14045087531169287 2202 26-27 0.1859936434354026 2202 28-29 0.11141682745365245 2202 30-31 0.24565271187525184 2202 32-33 0.24612177238542188 2202 34-35 0.36041669392204767 2202 36-37 0.32090380050996004 2202 38-39 0.33220343413087505 2202 40-41 0.3479444244723098 2202 42-43 0.3960328052986597 2202 44-45 0.4190136502009949 2202 46-47 0.3662995055927425 2202 48-49 0.32236826003553887 2202 50-51 0.2664831650174406 2202 52-53 0.28981937824956816 2202 54-55 0.49015987779552006 2202 56-57 0.4107673720704952 2202 58-59 0.31942544184278887 2202 60-61 0.2977694209550492 2202 62-63 0.12014852328744752 2202 64-65 0.26793536892098047 2202 66-67 0.2712454566486713 2202 68-69 0.23054845524641365 2202 70-71 0.4189103133846075 2202 72-73 0.2475389869034501 2202 74-75 0.2792067732704169 2202 76-77 0.04213106719837967 2202 78-79 0.4599953297015915 2202 80-81 0.4048115853798464 2202 82-83 0.46538799573285416 2202 84-85 0.45272912181017233 2202 86-87 0.4449968989192783 2202 88-89 0.6105592711514589 2202 90-91 0.6414827565725929 2202 92-93 0.48479051988072186 2202 94-95 0.47678022445098733 2202 96-97 0.23311652528488125 2202 98-99 0.1281295242177869 2202 100-101 0.20619023807548587 1102 1 0.09336175208778741 1102 2 0.13612969890979798 1102 3 0.08574559855180297 1102 4 0.01093847970830808 1102 5 0.0847872572578936 1102 6 0.16226051818414078 1102 7 0.10676624121196454 1102 8 0.07955059719891722 1102 9 0.1032227307654594 1102 10-11 0.0850733703219646 1102 12-13 0.08647130438063044 1102 14-15 0.22574911015770738 1102 16-17 0.09074297537571141 1102 18-19 0.13215560121336267 1102 20-21 0.1726052634762283 1102 22-23 0.15232998200481518 1102 24-25 0.18543675101791024 1102 26-27 0.1471282950361612 1102 28-29 0.21948336857103357 1102 30-31 0.1672733438066203 1102 32-33 0.16524449680475328 1102 34-35 0.19944944135874465 1102 36-37 0.1799851982919236 1102 38-39 0.06131255737842167 1102 40-41 0.20242496433420598 1102 42-43 0.17901826250727737 1102 44-45 0.16358364392353764 1102 46-47 0.20272710550904094 1102 48-49 0.21402446426235855 1102 50-51 0.21974744624782971 1102 52-53 0.12248311543307722 1102 54-55 0.2253869127400705 1102 56-57 0.27474257608807306 1102 58-59 0.2282426630187686 1102 60-61 0.04797082292198951 1102 62-63 0.13644377333975655 1102 64-65 0.07451885919090984 1102 66-67 0.047147842084953595 1102 68-69 0.06865789236714903 1102 70-71 0.07642757641034592 1102 72-73 0.15026706809197776 1102 74-75 0.09954052510134659 1102 76-77 -0.08229872983028486 1102 78-79 0.28280344855491535 1102 80-81 0.307034853846055 1102 82-83 0.21203478376381923 1102 84-85 0.13077369180389553 1102 86-87 0.11638997278409846 1102 88-89 0.1790097838110114 1102 90-91 0.27589895242947193 1102 92-93 0.28674961174094094 1102 94-95 0.2287550099709712 1102 96-97 0.059225543908024036 1102 98-99 -0.01979368147377869 1102 100-101 0.0905331968534746 2305 1 0.08778064019032428 2305 2 0.07722289249768721 2305 3 0.07746422041904566 2305 4 0.016785826903849 2305 5 0.13438453424370778 2305 6 0.006536459275366724 2305 7 0.03755264636142641 2305 8 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1213 86-87 -1.444777797881379 1213 88-89 -1.5172591778993372 1213 90-91 -1.3345011940626819 1213 92-93 -1.3665089097498786 1213 94-95 -1.4724995944471466 1213 96-97 -1.578056442865222 1213 98-99 -1.488250365443502 1213 100-101 -1.4836892270608395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 42.0 9 158.0 10 233.0 11 169.0 12 234.0 13 416.0 14 969.0 15 2085.0 16 3778.0 17 5833.0 18 8065.0 19 10609.0 20 13451.0 21 16695.0 22 20212.0 23 24613.0 24 29896.0 25 35847.0 26 43177.0 27 52792.0 28 65581.0 29 83774.0 30 97498.0 31 114266.0 32 145830.0 33 197837.0 34 283780.0 35 437390.0 36 703958.0 37 1013104.0 38 387368.0 39 1197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25321378906073 24.690033959204673 15.019936931399455 16.036815320335144 2 36.93333249141905 21.806106088566224 28.532106664225115 12.72845475578961 3 43.07651790567508 19.00247891136274 22.275289131781157 15.645714051181022 4 35.681675437855596 20.01478222270637 28.221731235615422 16.08181110382262 5 38.1102760373831 17.69188147320266 27.935926113602648 16.26191637581159 6 34.46865474200326 18.359151160973482 30.482243238507472 16.68995085851578 7 40.11474239264672 19.087117606479794 21.375651102302584 19.422488898570904 8 34.403669483396136 26.592467708640825 20.25064314358164 18.753219664381398 9 34.33602693283787 21.63456605370045 23.406085251187758 20.623321762273928 10-11 28.801387401801236 28.629606578766136 27.86414396964197 14.704862049790654 12-13 28.378878264983047 28.13259202780843 26.87817921303115 16.61035049417737 14-15 27.187622176232658 28.68149382955761 27.168376541952853 16.962507452256883 16-17 27.042616005003094 28.09515716093957 27.375265368342706 17.486961465714636 18-19 25.727433863011566 28.390027943668557 27.986882445845822 17.89565574747406 20-21 25.67504423996451 28.412660075530233 28.18694811861099 17.72534756589426 22-23 25.04683148191121 29.78825592720809 27.228218230496136 17.936694360384564 24-25 24.411567691749923 28.779497913894268 28.111123213628908 18.697811180726898 26-27 24.463016547299006 28.409081939833648 27.58155913053407 19.546342382333272 28-29 24.67332410384802 29.299327363108684 27.367647128467176 18.659701404576122 30-31 23.878241304209414 28.543367267740845 28.30989391353856 19.268497514511182 32-33 23.86135039893582 29.13940311434617 27.501301020663206 19.497945466054805 34-35 23.488040344532372 29.22908014024187 28.35106878387061 18.93181073135515 36-37 23.565956880307677 28.36507876371202 28.36297397800815 19.705990377972153 38-39 23.915857847848002 29.25775210395504 27.590308618661247 19.23608142953571 40-41 23.135288240103723 29.577074191428608 27.860782782752498 19.42685478571518 42-43 23.26133306075552 28.935240273004048 28.171548795836188 19.631877870404246 44-45 22.933384620727615 28.49466849645496 28.32837473334589 20.243572149471532 46-47 23.590029511316178 28.211223772396604 27.72708663107908 20.47166008520814 48-49 22.89192258817552 29.072401866002657 28.068923535806384 19.966752010015444 50-51 23.218112600302003 28.307469754491798 28.380575728290474 20.093841916915725 52-53 22.8157170127309 28.35120553492282 27.66887816249824 21.16419928984804 54-55 22.957995267239152 28.491957135423142 28.518101132665326 20.03194646467238 56-57 22.577575147531924 28.24605101282228 28.45139176715943 20.724982072486362 58-59 22.396448018438893 28.67269532602355 28.261607305342434 20.669249350195123 60-61 22.793992352079613 28.41813894814186 28.49452341160778 20.293345288170748 62-63 22.34558241842674 28.559847185853933 28.16464227041468 20.929928125304652 64-65 22.700789383419938 28.578082278653387 28.306665531651976 20.414462806274702 66-67 22.344261229499505 29.258607109689578 27.705844442415188 20.69128721839573 68-69 22.197639205826167 29.588101866850113 27.5230714693816 20.69118745794212 70-71 22.08873776723903 29.372469395892082 27.766633725772984 20.772159111095906 72-73 22.087802790759852 29.440386189016355 27.603533101079226 20.86827791914457 74-75 21.7797141781467 29.519282862203156 27.892613560174485 20.808389399475654 76-77 21.94197904253393 29.191072127875493 27.853688821421503 21.013260008169073 78-79 21.818000481308253 29.500130195329838 27.740960371804707 20.940908951557205 80-81 21.753821909999203 29.315928728635804 27.910043669386187 21.02020569197881 82-83 21.88458724771162 29.378092864775677 27.575175598616546 21.162144288896148 84-85 21.647791348313955 29.44422050048493 27.80627508515864 21.10171306604248 86-87 21.897283682766613 28.980559611694893 27.763033563253604 21.359123142284894 88-89 21.83415686291022 29.37503157974517 27.48078635670796 21.310025200636648 90-91 21.69857360543079 28.965887292368713 27.729188319424914 21.606350782775582 92-93 21.809117365920567 29.056068103538244 27.53702038860468 21.597794141936504 94-95 21.512373032729517 29.121973073160053 27.79725456679448 21.56839932731596 96-97 21.62974038473939 28.872930093298947 27.74396783582712 21.753361686134543 98-99 21.515276899310994 29.147133118711068 27.59739926453576 21.74019071744218 100-101 21.61613938956134 28.880754538097836 27.690743821513266 21.81236225082756 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 5.5 2 45.0 3 98.0 4 76.0 5 33.0 6 24.0 7 30.0 8 39.0 9 35.0 10 30.0 11 33.5 12 36.5 13 44.5 14 57.5 15 83.5 16 117.5 17 171.5 18 270.0 19 420.0 20 661.5 21 1062.5 22 1708.0 23 2708.0 24 4181.0 25 6304.0 26 9062.0 27 12781.0 28 17729.5 29 23616.5 30 30617.5 31 39200.0 32 49331.5 33 61149.0 34 74309.0 35 89329.5 36 106383.0 37 123912.0 38 140200.0 39 154214.0 40 166856.0 41 177604.0 42 184490.5 43 188188.5 44 190129.0 45 190218.5 46 188719.0 47 185760.0 48 181478.5 49 173894.5 50 162693.5 51 148856.5 52 131449.0 53 112340.5 54 93575.0 55 76318.0 56 61860.5 57 50129.5 58 40534.0 59 32616.0 60 25960.0 61 20377.0 62 15618.5 63 11929.0 64 9216.0 65 7053.5 66 5320.5 67 3986.5 68 3075.5 69 2380.5 70 1811.0 71 1418.0 72 1149.5 73 902.0 74 674.5 75 506.0 76 380.0 77 288.5 78 217.5 79 165.0 80 134.5 81 111.5 82 82.5 83 65.5 84 54.0 85 38.5 86 34.5 87 26.5 88 15.5 89 13.5 90 11.5 91 7.5 92 7.0 93 3.5 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18148514731658238 2 0.0 3 0.0 4 0.009234747275749553 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002328419184911212 12-13 0.0 14-15 0.0 16-17 3.420276768796131E-4 18-19 2.6309821298431775E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002723066504387689 40-41 0.0066958495204508875 42-43 0.0029598548960735748 44-45 0.0015259696353090433 46-47 0.007524608891351489 48-49 0.008721705760430134 50-51 0.005656611579162832 52-53 0.007024722286681285 54-55 0.019850760169666776 56-57 0.022021320426787398 58-59 0.028085734236075924 60-61 0.02645452531557315 62-63 0.011273758426378016 64-65 0.016246314651781623 66-67 0.023494670419499576 68-69 0.02733590432907062 70-71 0.02965116860333261 72-73 0.02256067176340525 74-75 0.03308460028277796 76-77 0.03067725163397145 78-79 0.0211136315919915 80-81 0.00982671825496427 82-83 0.0020390111506284627 84-85 0.008103424959916988 86-87 0.028059424414777493 88-89 0.025204808803897645 90-91 0.021745067303153863 92-93 0.044871400224475395 94-95 0.0461211167361509 96-97 0.035649807859375056 98-99 0.04285869889514537 100-101 0.04237196720112438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3800862.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.59709323099887 #Duplication Level Percentage of deduplicated Percentage of total 1 96.950949903676 90.74327096968263 2 2.283706107282485 4.274965068710404 3 0.39317144314079494 1.1039911265824605 4 0.15588811942919356 0.5836269939127726 5 0.04515603915096845 0.2113237003177914 6 0.05318124787327726 0.2986566129201595 7 0.019013494203019135 0.12457254526969269 8 0.004996346215100297 0.037411478600727265 9 0.013337743862690501 0.11235366502266832 >10 0.07713790375280451 1.3042838219308541 >50 0.002711415763669301 0.15867129785619216 >100 6.511388315515726E-4 0.10473512372574487 >500 7.095527650314722E-5 0.04938098126915157 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.8141541950820694E-5 0.8927566141987371 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 33894 0.8917450830890467 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010260830306388392 0.0 0.0 2 0.0013154910649215887 0.0 2.630982129843178E-5 3 0.001894307133487088 0.0 2.630982129843178E-5 4 0.002446813380754155 0.0 2.630982129843178E-5 5 0.003051939270618086 0.0 5.261964259686356E-5 6 0.0056303017578644005 0.0 1.3154910649215888E-4 7 0.007919256210827964 0.0 1.3154910649215888E-4 8 0.010444999055477416 0.0 1.3154910649215888E-4 9 0.01170787047780214 0.0 1.3154910649215888E-4 10-11 0.015693808404514557 0.0 1.3154910649215888E-4 12-13 0.020732139183164237 0.0 1.3154910649215888E-4 14-15 0.024362894522347828 0.0 1.5785892779059067E-4 16-17 0.02832252262776181 0.0 1.5785892779059067E-4 18-19 0.03301882572953188 0.0 1.5785892779059067E-4 20-21 0.038754366772590004 0.0 1.7101383843980654E-4 22-23 0.044871400224475395 0.0 2.1047857038745423E-4 24-25 0.050462237250392145 0.0 2.36788391685886E-4 26-27 0.055934680080465954 0.0 2.6309821298431776E-4 28-29 0.06114402469755545 0.0 2.6309821298431776E-4 30-31 0.08808528170714959 0.0 2.6309821298431776E-4 32-33 0.09664912853978913 0.0 2.8940803428274955E-4 34-35 0.10301610529400962 0.0 2.8940803428274955E-4 36-37 0.10969879990381129 0.0 3.157178555811813E-4 38-39 0.1177364503104822 0.0 3.55182587528829E-4 40-41 0.12716852124597 0.0 4.3411205142412435E-4 42-43 0.13812656181676683 0.0 4.472669620733402E-4 44-45 0.15124200773403507 0.0 4.472669620733402E-4 46-47 0.16634384515933492 0.0 4.472669620733402E-4 48-49 0.1819850339212526 0.0 4.604218727225561E-4 50-51 0.20042821865145327 0.0 4.73576783371772E-4 52-53 0.220397373016963 0.0 4.8673169402098783E-4 54-55 0.24139261041311155 0.0 5.656611579162832E-4 56-57 0.2635849446783387 0.0 5.788160685654991E-4 58-59 0.2901841740110533 0.0 5.788160685654991E-4 60-61 0.31849354172816585 0.0 5.91970979214715E-4 62-63 0.34915763845148806 0.0 6.051258898639309E-4 64-65 0.3815844932018053 0.0 6.051258898639309E-4 66-67 0.41873396087519094 0.0 6.051258898639309E-4 68-69 0.4589879874617916 0.0 6.314357111623627E-4 70-71 0.5025833613532931 0.0 6.314357111623627E-4 72-73 0.5498489553159257 0.0 6.445906218115786E-4 74-75 0.6012978108650091 0.0 6.840553537592262E-4 76-77 0.6557459860421135 0.0 7.498299070053056E-4 78-79 0.7137328321838572 0.0 7.629848176545215E-4 80-81 0.7746400684897268 0.0 7.892946389529533E-4 82-83 0.8418221971752724 0.0 7.892946389529533E-4 84-85 0.9125298419148078 0.0 7.892946389529533E-4 86-87 0.9906437013498517 0.0 8.024495496021692E-4 88-89 1.072743498711608 0.0 8.156044602513851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 9310 0.0 42.390244 3 CGGAAGA 9530 0.0 41.262165 4 ATCGGAA 9760 0.0 40.338455 2 GATCGGA 9875 0.0 39.895657 1 ATGCCGT 4995 0.0 38.655224 48-49 GCCGTCT 5060 0.0 38.48899 50-51 CGTATGC 5055 0.0 38.05246 46-47 TATGCCG 5055 0.0 38.00848 48-49 TGCCGTC 5075 0.0 37.907234 50-51 CTCGTAT 5120 0.0 37.38335 44-45 CGTCTTC 5220 0.0 37.22021 52-53 TCTCGTA 5140 0.0 36.913998 42-43 AGAGCAC 10580 0.0 36.851482 8 GAGCACA 10535 0.0 36.82858 9 CCGTCTT 5275 0.0 36.69705 52-53 CGGACCG 65 1.3155298E-4 36.60067 1 TCGTATG 5345 0.0 36.431686 44-45 ATCTCGT 5190 0.0 36.28384 42-43 AATCTCG 5180 0.0 36.170753 40-41 GAAGAGC 11025 0.0 35.277905 6 >>END_MODULE