FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3800862
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT338940.8917450830890467TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG93100.042.3902443
CGGAAGA95300.041.2621654
ATCGGAA97600.040.3384552
GATCGGA98750.039.8956571
ATGCCGT49950.038.65522448-49
GCCGTCT50600.038.4889950-51
CGTATGC50550.038.0524646-47
TATGCCG50550.038.0084848-49
TGCCGTC50750.037.90723450-51
CTCGTAT51200.037.3833544-45
CGTCTTC52200.037.2202152-53
TCTCGTA51400.036.91399842-43
AGAGCAC105800.036.8514828
GAGCACA105350.036.828589
CCGTCTT52750.036.6970552-53
CGGACCG651.3155298E-436.600671
TCGTATG53450.036.43168644-45
ATCTCGT51900.036.2838442-43
AATCTCG51800.036.17075340-41
GAAGAGC110250.035.2779056
Produced by FastQC (version 0.11.2)