##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2776572 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.662147064797885 33.0 33.0 33.0 33.0 33.0 2 32.66414016996498 33.0 33.0 33.0 33.0 33.0 3 32.72565955429933 33.0 33.0 33.0 33.0 33.0 4 36.65692803932331 37.0 37.0 37.0 37.0 37.0 5 36.61770917519877 37.0 37.0 37.0 37.0 37.0 6 36.526571254049955 37.0 37.0 37.0 37.0 37.0 7 36.418886670325854 37.0 37.0 37.0 37.0 37.0 8 36.3750466402456 37.0 37.0 37.0 37.0 37.0 9 36.4195835728373 37.0 37.0 37.0 37.0 37.0 10-11 36.345724872252546 37.0 37.0 37.0 37.0 37.0 12-13 36.34807957438165 37.0 37.0 37.0 37.0 37.0 14-15 38.2059429757269 40.0 37.0 40.0 37.0 40.0 16-17 38.22914100552768 40.0 37.0 40.0 37.0 40.0 18-19 38.17543431252638 40.0 37.0 40.0 37.0 40.0 20-21 38.1679897009694 40.0 37.0 40.0 37.0 40.0 22-23 37.96471620400983 40.0 37.0 40.0 33.0 40.0 24-25 38.042179529290074 40.0 37.0 40.0 35.0 40.0 26-27 37.985975512250356 40.0 37.0 40.0 35.0 40.0 28-29 37.78850107254557 40.0 37.0 40.0 33.0 40.0 30-31 37.72787811733318 40.0 37.0 40.0 33.0 40.0 32-33 37.646544371980994 40.0 37.0 40.0 33.0 40.0 34-35 37.52819177748677 40.0 37.0 40.0 33.0 40.0 36-37 37.49044667309185 40.0 37.0 40.0 33.0 40.0 38-39 37.44916411315824 40.0 37.0 40.0 33.0 40.0 40-41 37.37846110239533 40.0 37.0 40.0 33.0 40.0 42-43 36.96654183648038 38.5 37.0 40.0 33.0 40.0 44-45 36.81367600047828 37.0 37.0 40.0 33.0 40.0 46-47 37.02244638352616 37.0 37.0 40.0 33.0 40.0 48-49 36.76499889071849 37.0 37.0 40.0 33.0 40.0 50-51 36.69295015580363 37.0 37.0 40.0 33.0 40.0 52-53 36.63218493883825 37.0 37.0 40.0 33.0 40.0 54-55 36.26808795161804 37.0 37.0 40.0 33.0 40.0 56-57 36.184974493728234 37.0 37.0 40.0 33.0 40.0 58-59 36.0285447306967 37.0 37.0 40.0 33.0 40.0 60-61 35.74266685682922 37.0 35.0 40.0 30.0 40.0 62-63 35.371483613607 37.0 33.0 38.5 27.0 40.0 64-65 35.12334670233655 37.0 33.0 37.0 27.0 40.0 66-67 34.56415068653001 37.0 33.0 37.0 27.0 40.0 68-69 34.34341807091622 37.0 33.0 37.0 27.0 40.0 70-71 33.847756874304 37.0 33.0 37.0 27.0 40.0 72-73 33.0834159892126 37.0 33.0 37.0 24.5 37.0 74-75 32.3240978443923 37.0 33.0 37.0 22.0 37.0 76-77 29.800633659058725 33.0 27.0 35.0 18.5 37.0 78-79 31.806068238100796 33.0 33.0 37.0 18.5 37.0 80-81 32.09095694258964 37.0 33.0 37.0 15.0 37.0 82-83 32.035297481930954 37.0 33.0 37.0 15.0 37.0 84-85 31.873350123821748 37.0 33.0 37.0 15.0 37.0 86-87 31.73510879602618 35.0 33.0 37.0 15.0 37.0 88-89 31.520166773993253 33.0 33.0 37.0 15.0 37.0 90-91 31.26892297408459 33.0 33.0 37.0 15.0 37.0 92-93 31.250940548273192 33.0 33.0 37.0 15.0 37.0 94-95 31.18351207892322 33.0 33.0 37.0 15.0 37.0 96-97 31.024063305399608 33.0 33.0 37.0 15.0 37.0 98-99 30.864066013775258 33.0 33.0 37.0 15.0 37.0 100-101 29.350460387845153 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.12470260365959973 2306 2 0.12593217297583692 2306 3 0.122127439092516 2306 4 0.13371361055475717 2306 5 0.16308148007241385 2306 6 0.18058721499728136 2306 7 0.1447565133289146 2306 8 0.16562433350050298 2306 9 0.25349406628401283 2306 10-11 0.15423581773920603 2306 12-13 0.20924193862028773 2306 14-15 0.45338907973211207 2306 16-17 0.44950183434989555 2306 18-19 0.4574205793417576 2306 20-21 0.40085062249377756 2306 22-23 0.38088070599241775 2306 24-25 0.39473598777624375 2306 26-27 0.4282484550709782 2306 28-29 0.46839769224703787 2306 30-31 0.43861137640639214 2306 32-33 0.4501533871636738 2306 34-35 0.48021912523133636 2306 36-37 0.5179867130328475 2306 38-39 0.5865170572344383 2306 40-41 0.5327264742710156 2306 42-43 0.49053551991855926 2306 44-45 0.48082985568567693 2306 46-47 0.4891681620265871 2306 48-49 0.5083448024267341 2306 50-51 0.360168494471786 2306 52-53 0.26575584508416483 2306 54-55 0.5142563787712788 2306 56-57 0.5399182200080901 2306 58-59 0.44705990730532363 2306 60-61 0.3451596370129053 2306 62-63 0.28860565626001033 2306 64-65 0.29033014513473177 2306 66-67 0.4154070941960697 2306 68-69 0.4882399691369841 2306 70-71 0.4403829626161482 2306 72-73 0.37268364531521314 2306 74-75 0.2952080066453746 2306 76-77 -0.05577329950010679 2306 78-79 0.4389030128268807 2306 80-81 0.5555848256996114 2306 82-83 0.5963690305870628 2306 84-85 0.5908498836167198 2306 86-87 0.4704387358724951 2306 88-89 0.7315961347680364 2306 90-91 0.6590338417681316 2306 92-93 0.6167693565293639 2306 94-95 0.6304338987728819 2306 96-97 0.5854498840658025 2306 98-99 0.4422999204492868 2306 100-101 0.5055764791665069 2307 1 0.11282734405093464 2307 2 0.1790208704334617 2307 3 0.08518231890421646 2307 4 0.10935259664675812 2307 5 0.1947015430058059 2307 6 0.1369796880825831 2307 7 0.16136058367610673 2307 8 0.19523322705259005 2307 9 0.18428395538374076 2307 10-11 0.12829741220093638 2307 12-13 0.23021149126125096 2307 14-15 0.380535295090489 2307 16-17 0.2909342983005345 2307 18-19 0.34256080215549645 2307 20-21 0.3414104055574114 2307 22-23 0.4143604525094844 2307 24-25 0.3435923640024541 2307 26-27 0.2421710585923762 2307 28-29 0.3045975141844366 2307 30-31 0.4238544235446966 2307 32-33 0.37641364312816705 2307 34-35 0.3915538249382067 2307 36-37 0.4491399305773385 2307 38-39 0.36992848177680315 2307 40-41 0.24818746504551825 2307 42-43 0.22922044113506246 2307 44-45 0.36548000067074327 2307 46-47 0.3173946462559343 2307 48-49 0.38226696267160065 2307 50-51 0.32114729655257435 2307 52-53 0.1263692210723164 2307 54-55 0.39283917619845 2307 56-57 0.3817430034803664 2307 58-59 0.4026256894440152 2307 60-61 0.3955022957103509 2307 62-63 0.3402821023853875 2307 64-65 0.4408759590507998 2307 66-67 0.346682014896075 2307 68-69 0.3379535907280342 2307 70-71 0.4275582205031583 2307 72-73 0.22197793522083487 2307 74-75 0.31532395512159894 2307 76-77 -3.5176033712147614E-4 2307 78-79 0.41222736321403275 2307 80-81 0.5670389726010612 2307 82-83 0.4691732038773111 2307 84-85 0.4018862423849612 2307 86-87 0.3509870887340689 2307 88-89 0.4627000377981254 2307 90-91 0.5851400393479622 2307 92-93 0.3919111011272811 2307 94-95 0.47796052455388605 2307 96-97 0.28186393521951913 2307 98-99 0.2153932920996553 2307 100-101 0.3781734602554181 2201 1 0.15043225549720063 2201 2 0.2208543155289462 2201 3 0.1332128082714661 2201 4 0.1078089960138442 2201 5 0.13012711149273315 2201 6 0.15420609001196084 2201 7 0.19244618341618036 2201 8 0.10509966720123032 2201 9 0.0874273081650756 2201 10-11 0.15485994698902772 2201 12-13 0.09128715127740605 2201 14-15 0.2652432713604398 2201 16-17 0.3223259388946147 2201 18-19 0.2064724344626896 2201 20-21 0.20154888621362943 2201 22-23 0.21984809332695932 2201 24-25 0.371871708735398 2201 26-27 0.3246168986065996 2201 28-29 0.32165561251223096 2201 30-31 0.35695676081379446 2201 32-33 0.37910782152168565 2201 34-35 0.394903104345957 2201 36-37 0.44815709037481355 2201 38-39 0.27648639764990435 2201 40-41 0.3789920053181959 2201 42-43 0.41953058769126983 2201 44-45 0.3778836176132572 2201 46-47 0.4690662859292445 2201 48-49 0.5005915644694312 2201 50-51 0.5753657357593127 2201 52-53 0.4719388740461383 2201 54-55 0.5543984637836488 2201 56-57 0.5660909194780004 2201 58-59 0.5481137755490479 2201 60-61 0.49967675333690664 2201 62-63 0.5023883766757322 2201 64-65 0.5204708266211497 2201 66-67 0.5782848463211536 2201 68-69 0.2792492190231144 2201 70-71 0.5964825831287399 2201 72-73 0.18559105505262607 2201 74-75 0.24436030756745453 2201 76-77 0.25619887244291206 2201 78-79 0.5175892857694642 2201 80-81 0.49085895114423295 2201 82-83 0.3823494890696537 2201 84-85 0.4694707989068547 2201 86-87 0.5323903520880755 2201 88-89 0.5940812820598573 2201 90-91 0.6144969422296178 2201 92-93 0.4547196655918988 2201 94-95 0.3648681458768195 2201 96-97 0.47335003715338786 2201 98-99 0.47072466717403927 2201 100-101 0.631862837467942 1101 1 0.12726609664454713 1101 2 0.1622768273281494 1101 3 0.12206514860876894 1101 4 0.11007289764201289 1101 5 0.15693020265676694 1101 6 0.15161992855873052 1101 7 0.14540503466709254 1101 8 0.1583811297055746 1101 9 0.1389175402132352 1101 10-11 0.23794464474305244 1101 12-13 0.2295225590924801 1101 14-15 0.30222970271066174 1101 16-17 0.1686767065899133 1101 18-19 0.32629229227477197 1101 20-21 0.34320861216978216 1101 22-23 0.28147246558800987 1101 24-25 0.2674604039872506 1101 26-27 0.29757149757409707 1101 28-29 0.26363882829277685 1101 30-31 0.3104246560740762 1101 32-33 0.1737269258713141 1101 34-35 0.09889585789274946 1101 36-37 0.19686664536105525 1101 38-39 0.3044859267358717 1101 40-41 0.2803174174558336 1101 42-43 0.2393676654940009 1101 44-45 0.31108727387412927 1101 46-47 0.43295385696170285 1101 48-49 0.5106302918769785 1101 50-51 0.3028834565354117 1101 52-53 0.16905364704097536 1101 54-55 0.42942613876862623 1101 56-57 0.3452714236371577 1101 58-59 0.26375725848543397 1101 60-61 0.32250958237050753 1101 62-63 0.3085429419061043 1101 64-65 0.40288130902689545 1101 66-67 0.21848807735368325 1101 68-69 0.25842761461198194 1101 70-71 0.23264069778576157 1101 72-73 0.1217335311467096 1101 74-75 0.26817660892431405 1101 76-77 0.16498159481800556 1101 78-79 0.4309908313514832 1101 80-81 0.4508850271300844 1101 82-83 0.3357202143826896 1101 84-85 0.4406020117213387 1101 86-87 0.4250291575127214 1101 88-89 0.4058919113626622 1101 90-91 0.4590520866139691 1101 92-93 0.3656612125093446 1101 94-95 0.23400306977500662 1101 96-97 0.1802172991397306 1101 98-99 0.2887337902548026 1101 100-101 0.2496688349446856 2304 1 0.09517724742048728 2304 2 0.13781850019244501 2304 3 0.15812543915366462 2304 4 0.07961526464087143 2304 5 0.10049706268949166 2304 6 0.09353573995245767 2304 7 0.11131311141853928 2304 8 0.17341905920133627 2304 9 0.09900976094802161 2304 10-11 0.054041129447298886 2304 12-13 0.09506125709436475 2304 14-15 0.23208244702123437 2304 16-17 0.27336459394178547 2304 18-19 0.2558153226679494 2304 20-21 0.19277973617195698 2304 22-23 0.33408042830306783 2304 24-25 0.3235489557250659 2304 26-27 0.245625441745851 2304 28-29 0.39955285476905544 2304 30-31 0.26580667798889834 2304 32-33 0.2877186954186399 2304 34-35 0.32924937070945504 2304 36-37 0.4071745436207621 2304 38-39 0.4796433465385448 2304 40-41 0.4412897016543553 2304 42-43 0.4352945830407293 2304 44-45 0.4733808927942533 2304 46-47 0.3971811364500297 2304 48-49 0.5342184717569864 2304 50-51 0.45703091054899403 2304 52-53 0.3957356838301038 2304 54-55 0.512380177144351 2304 56-57 0.5214873986483184 2304 58-59 0.5181303062319813 2304 60-61 0.41596736411477053 2304 62-63 0.5017924832814842 2304 64-65 0.5057396559950149 2304 66-67 0.3629721866302944 2304 68-69 0.33015154738009045 2304 70-71 0.5229699158927588 2304 72-73 0.3413973716940788 2304 74-75 0.33872547103944584 2304 76-77 0.4083922745740658 2304 78-79 0.497754181046556 2304 80-81 0.605954688491579 2304 82-83 0.4531702614846793 2304 84-85 0.6668130286284679 2304 86-87 0.5583971975742053 2304 88-89 0.7263812762295352 2304 90-91 0.6775482936643904 2304 92-93 0.507170459373345 2304 94-95 0.4322726914716668 2304 96-97 0.45462852428723366 2304 98-99 0.3004745805765836 2304 100-101 0.6276722064554221 2202 1 0.11976441548289074 2202 2 0.12926042112088254 2202 3 0.07153827737127472 2202 4 0.030743624836937045 2202 5 0.12478027842970363 2202 6 0.13292959370993884 2202 7 0.08112449141860623 2202 8 0.14642445058705533 2202 9 0.14973714271904726 2202 10-11 0.12156643002411727 2202 12-13 0.056747564481845814 2202 14-15 0.20515299347601967 2202 16-17 0.1605722203818729 2202 18-19 0.17251056336490223 2202 20-21 0.1772150536734003 2202 22-23 0.10952309027229035 2202 24-25 0.19177981504977737 2202 26-27 0.13283181218459816 2202 28-29 0.10605815666467322 2202 30-31 0.1867431211815287 2202 32-33 0.26666057709703495 2202 34-35 0.23616632704142404 2202 36-37 0.217946482039018 2202 38-39 0.24812791813219093 2202 40-41 0.2015259555047848 2202 42-43 0.317213815875121 2202 44-45 0.22474522424044352 2202 46-47 0.3214247881482919 2202 48-49 0.21814934772371686 2202 50-51 0.22068301702022808 2202 52-53 0.21848092594006374 2202 54-55 0.30185975299269785 2202 56-57 0.3804001410958193 2202 58-59 0.35111572719652173 2202 60-61 0.34552798344903124 2202 62-63 0.28840021208567634 2202 64-65 0.2197370058322008 2202 66-67 0.05574358496797771 2202 68-69 0.13344619587242335 2202 70-71 0.34672545407362776 2202 72-73 0.19695674758702353 2202 74-75 0.2551673134678012 2202 76-77 0.010080968364082565 2202 78-79 0.32922392240448417 2202 80-81 0.4465704745443091 2202 82-83 0.32279135728303743 2202 84-85 0.28327842108178913 2202 86-87 0.2502641179531473 2202 88-89 0.38540957079952776 2202 90-91 0.543943350043147 2202 92-93 0.46658528835306257 2202 94-95 0.38762890914925663 2202 96-97 0.1307016989247991 2202 98-99 0.14204382863998433 2202 100-101 0.1756737513370581 1102 1 0.12945231063631724 1102 2 0.11338279440634125 1102 3 0.08916905807379294 1102 4 0.038519399699630696 1102 5 0.16981600512590234 1102 6 0.1665119038673737 1102 7 0.13105318281523637 1102 8 0.1268996482240965 1102 9 0.1567416142873057 1102 10-11 0.1494672784879043 1102 12-13 0.02889241505955198 1102 14-15 0.22433618419214696 1102 16-17 0.14979810576686248 1102 18-19 0.16538694248053076 1102 20-21 0.16916025826031955 1102 22-23 0.13495754789254022 1102 24-25 0.22098406653869063 1102 26-27 0.2262237609485851 1102 28-29 0.177180494959444 1102 30-31 0.2520656025761312 1102 32-33 0.16877579418407151 1102 34-35 0.1651252817610569 1102 36-37 0.2203234354845165 1102 38-39 0.20608057694163762 1102 40-41 0.19689406354636674 1102 42-43 0.10656159553515465 1102 44-45 0.14939745905931545 1102 46-47 0.2705695977024405 1102 48-49 0.2776570408481689 1102 50-51 0.1660907610621578 1102 52-53 0.23962977872821511 1102 54-55 0.2963242869167715 1102 56-57 0.23296432487173035 1102 58-59 0.20357207330025062 1102 60-61 0.04992727784376427 1102 62-63 0.2510069336756544 1102 64-65 0.22829283166063874 1102 66-67 0.23437027899976925 1102 68-69 0.04696928127865618 1102 70-71 0.021619607250791262 1102 72-73 -0.1036136910755161 1102 74-75 0.14240500398604894 1102 76-77 -0.0689690224659465 1102 78-79 0.18133805357370036 1102 80-81 0.34869881313831286 1102 82-83 0.2049974777571748 1102 84-85 0.24556600349088598 1102 86-87 0.1907055978419443 1102 88-89 0.27371598543673414 1102 90-91 0.32024035821890706 1102 92-93 0.3097455746492628 1102 94-95 0.20926027142109405 1102 96-97 0.10763499461298309 1102 98-99 -0.02008822620610573 1102 100-101 0.06775011066484993 2305 1 0.07342687665516934 2305 2 0.09853305793998857 2305 3 0.05175798346255078 2305 4 0.03190609400518696 2305 5 0.1439867522024727 2305 6 0.014133415145607842 2305 7 0.1150578038364074 2305 8 0.03869048331355174 2305 9 -0.02336041891609142 2305 10-11 0.016965866700516585 2305 12-13 0.08960723572812412 2305 14-15 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757.0 16 1534.0 17 2405.0 18 2956.0 19 3857.0 20 5177.0 21 6854.0 22 9595.0 23 12358.0 24 16329.0 25 22219.0 26 29862.0 27 43597.0 28 71776.0 29 107019.0 30 84414.0 31 62721.0 32 79170.0 33 111241.0 34 171076.0 35 296827.0 36 569412.0 37 845466.0 38 219052.0 39 458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96330931280458 24.599616763546138 18.42201253618323 15.015061387466053 2 29.61889697079708 28.40102831837244 27.55606553692827 14.424009173902208 3 35.85165448617936 18.5973207249803 28.592703520744283 16.958321268096054 4 29.937236722910487 18.486045301218706 26.9922793064883 24.584438669382507 5 38.044142201246714 16.702682300332928 27.490877239992333 17.762298258428018 6 34.84930338561363 17.461459670413735 29.80545795318832 17.883778990784318 7 32.162104926506494 24.91550732341895 23.089118524569145 19.833269225505408 8 28.779840753274183 31.250081035175747 20.962755512913045 19.007322698637026 9 34.52606307345893 22.2540600423832 23.4252164179427 19.794660466215173 10-11 26.314917498179824 29.990389029122667 27.342878994509125 16.35181447818839 12-13 22.582468597969008 29.17385178558309 27.022205799093268 21.22147381735464 14-15 22.471594469727417 29.43791841162412 27.16068591054005 20.929801208108415 16-17 25.837951452929396 25.669001567765616 27.460839174187658 21.032207805117334 18-19 21.25340167659978 25.710462397517514 31.372047978586544 21.664087947296164 20-21 24.574871460203447 25.84298192159252 31.277596979296774 18.304549638907258 22-23 20.637876489426528 33.28964636969616 27.52143650515816 18.551040635719154 24-25 20.709097405001565 25.284217373077304 31.57949802850421 22.42718719341692 26-27 20.717200922576474 25.430224751960328 27.83835607360443 26.014218251858768 28-29 24.05997395349373 29.897351842487787 27.41762144111516 18.625052762903323 30-31 19.784936245125284 27.1410934058256 30.848074532192932 22.22589581685618 32-33 19.461623901703252 31.18588316816564 27.387422332286 21.965070597845113 34-35 19.737143499250152 30.948900298641636 31.47586304262954 17.838093159478667 36-37 19.621371244829955 31.127663896344128 31.67897320869043 17.57199165013549 38-39 19.81137528887346 27.182530928571158 31.227812816662304 21.778280965893078 40-41 23.644594694860473 26.574952042247485 27.371630621214077 22.40882264167797 42-43 20.024264392193544 30.3176143027733 30.91010559530689 18.748015709726268 44-45 19.566107223076504 26.5702420477872 31.432357231855416 22.431293497280876 46-47 23.311253674515417 26.400556982970492 27.812968403354503 22.475220939159588 48-49 19.54876629120887 30.863304403200758 30.907787363525557 18.680141942064814 50-51 23.33237826529269 27.011221119021588 30.369578915843498 19.286821699842225 52-53 19.486292155713706 26.86994647390887 26.85301809571593 26.790743274661494 54-55 23.63729300958245 26.622292097319782 30.776158118804513 18.964256774293247 56-57 19.98377516938089 26.559640661788652 30.413776408577547 23.042807760252913 58-59 19.813990368265426 27.06403576019229 30.54244433364651 22.579529537895773 60-61 23.148458360531954 27.0774162822835 30.605833978174818 19.168291379009723 62-63 19.484903534223932 27.11004646299358 30.297927580115303 23.107122422667185 64-65 23.293471131031605 27.019606670684226 30.410043128119657 19.27687907016451 66-67 20.04857448441845 34.02357325060349 26.620701505758582 19.30715075921948 68-69 20.281837944802863 34.05424751801295 26.6347197012231 19.029194835961086 70-71 19.816394281257374 34.43377278859854 26.649848601386633 19.09998432875745 72-73 19.66825626232003 34.342722970869985 26.5422007746487 19.446819992161295 74-75 20.087279136157758 33.385476486584245 26.583533043291546 19.943711333966448 76-77 20.427031750340582 32.32452453206152 26.635157040463252 20.613286677134653 78-79 20.13333172430848 31.92675742887675 27.166829640471203 20.77308120634357 80-81 19.72286601228501 31.680408225232732 27.46994617496854 21.126779587513717 82-83 19.43244903883486 31.406801160875137 27.400535777442748 21.760214022847254 84-85 19.45949109717202 31.19258454850137 27.85530720924662 21.492617145079986 86-87 19.598059827741984 31.033731819891376 28.29850178468757 21.06970656767907 88-89 19.964858117576707 31.32013516385736 28.028380087107195 20.68662663145874 90-91 20.366115989323507 30.72090455152196 27.46287786533564 21.45010159381889 92-93 20.04227565760933 31.069077264322125 27.257926550683948 21.630720527384597 94-95 19.690073647774504 31.067852194236707 27.544761304570365 21.697312853418428 96-97 19.87067789175601 31.541192036755657 27.196552851562394 21.391577219925946 98-99 20.06127328892107 31.209491703010826 27.07172516591546 21.657509842152642 100-101 20.144322828289308 31.11887122945532 26.858544640326983 21.878261301928394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64.0 1 45.5 2 27.0 3 44.0 4 50.5 5 34.5 6 31.0 7 37.5 8 48.0 9 49.5 10 49.5 11 50.0 12 62.5 13 81.5 14 93.0 15 103.5 16 123.5 17 156.5 18 189.0 19 240.5 20 308.5 21 438.5 22 660.0 23 970.0 24 1442.0 25 2165.0 26 3037.5 27 4270.5 28 5894.5 29 7700.5 30 10117.0 31 13441.5 32 19063.0 33 28696.5 34 43050.5 35 63908.5 36 93640.5 37 131579.5 38 167975.5 39 190296.0 40 201370.5 41 197731.0 42 185037.0 43 178026.0 44 166021.5 45 151764.5 46 146271.0 47 135823.5 48 114530.0 49 94457.5 50 77786.5 51 65299.0 52 56069.0 53 46990.0 54 38449.5 55 30626.0 56 24222.0 57 18567.5 58 13916.0 59 10479.0 60 8132.5 61 6371.0 62 4866.5 63 3575.5 64 2555.0 65 1903.5 66 1392.5 67 1021.0 68 764.5 69 572.5 70 403.5 71 300.5 72 250.0 73 201.5 74 147.0 75 108.5 76 90.0 77 69.5 78 53.0 79 39.5 80 29.5 81 22.5 82 13.0 83 8.0 84 6.5 85 8.5 86 7.0 87 5.0 88 3.5 89 2.0 90 2.5 91 2.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.177016839469677 2 0.0 3 0.0 4 0.009616174188891914 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0020708989358100565 12-13 0.0 14-15 0.0 16-17 3.2414070299635665E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001926836401145009 40-41 0.006122657723264514 42-43 0.00271918034180277 44-45 0.001206523727819772 46-47 0.006680900045091573 48-49 0.008211564475907701 50-51 0.005600431035103718 52-53 0.006086642089598252 54-55 0.01827793408562789 56-57 0.020889067526431875 58-59 0.026939693982363865 60-61 0.02506688103171825 62-63 0.009958322708721401 64-65 0.015306644308161286 66-67 0.022653833576078704 68-69 0.025985279690207926 70-71 0.02834430369534808 72-73 0.02175344273442216 74-75 0.03169375762631043 76-77 0.029046608551840183 78-79 0.020294809570938554 80-81 0.009418088203727474 82-83 0.0016567191486480453 84-85 0.007455236168916204 86-87 0.026507506378368725 88-89 0.024562662160390582 90-91 0.020402856471937337 92-93 0.04379501053817441 94-95 0.0456498156719869 96-97 0.03401676599778432 98-99 0.04096778329537286 100-101 0.04060762695871024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2776572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.112310099951074 #Duplication Level Percentage of deduplicated Percentage of total 1 90.53550393414977 47.18014256071754 2 4.482992104212122 4.672381494206686 3 1.5449110499078644 2.4152665112891887 4 0.680904933458937 1.4193411616399472 5 0.4340319997431292 1.130920508195792 6 0.34504894823306875 1.0788778673990187 7 0.25774026065564615 0.9402008271970476 8 0.20772546146617618 0.8660042930864649 9 0.12022058514859235 0.5638475172294916 >10 1.140248075474743 11.837385157788972 >50 0.12092837725942086 4.418531604771819 >100 0.12034759232763421 12.624705244650109 >500 0.007017060638331318 2.446925949259924 >1k 0.002241470389239475 1.663351816627996 >5k 6.907346795515594E-5 0.19725687340034498 >10k+ 6.907346730891623E-5 6.544860612539455 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 181823 6.548470560100729 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5480 0.19736567249111497 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004069766604287589 0.0 0.0 2 0.004537969841948993 0.0 0.0 3 0.005114219980609183 0.0 0.0 4 0.005438360683605539 0.0 0.0 5 0.00680695476292349 0.0 0.0 6 0.008139533208575179 0.0 0.0 7 0.010660627565213509 0.0 0.0 8 0.012173284179196506 0.0 0.0 9 0.014298206565505955 0.0 0.0 10-11 0.023608247868234643 0.0 0.0 12-13 0.03252211720063445 0.0 0.0 14-15 0.04174211941919748 0.0 0.0 16-17 0.05020579333076902 0.0 0.0 18-19 0.059317748648333266 0.0 0.0 20-21 0.06646685193108624 0.0 0.0 22-23 0.07318376760984407 0.0 0.0 24-25 0.08090912103125725 0.0 0.0 26-27 0.08885056825466799 0.0 1.8007816833130925E-5 28-29 0.09783646885440031 0.0 3.601563366626185E-5 30-31 0.1991484463575949 0.0 7.20312673325237E-5 32-33 0.21222212137844795 0.0 7.20312673325237E-5 34-35 0.22234251443866754 0.0 7.20312673325237E-5 36-37 0.23388552502870447 0.0 9.003908416565463E-5 38-39 0.24623888737623228 0.0 1.440625346650474E-4 40-41 0.26134744569922913 0.0 1.440625346650474E-4 42-43 0.2802556533740166 0.0 1.440625346650474E-4 44-45 0.30076655674695274 0.0 1.440625346650474E-4 46-47 0.32338437468936515 0.0 1.440625346650474E-4 48-49 0.34818113846858645 0.0 1.440625346650474E-4 50-51 0.37578712167377615 0.0 1.440625346650474E-4 52-53 0.40692263697825953 0.0 1.440625346650474E-4 54-55 0.44000299650072106 0.0 1.440625346650474E-4 56-57 0.4774772633304665 0.0 1.8007816833130925E-4 58-59 0.5200297345071548 0.0 1.8007816833130925E-4 60-61 0.5642569326493244 0.0 1.8007816833130925E-4 62-63 0.6118515925392894 0.0 1.8007816833130925E-4 64-65 0.6623815265730548 0.0 1.8007816833130925E-4 66-67 0.7194122824835805 3.601563366626185E-5 1.8007816833130925E-4 68-69 0.7782078044437529 3.601563366626185E-5 1.8007816833130925E-4 70-71 0.8393623504090656 3.601563366626185E-5 1.8007816833130925E-4 72-73 0.9047667411469971 3.601563366626185E-5 2.160938019975711E-4 74-75 0.9747811329942101 3.601563366626185E-5 2.160938019975711E-4 76-77 1.0517285343221785 3.601563366626185E-5 2.160938019975711E-4 78-79 1.1344924604872482 3.601563366626185E-5 2.160938019975711E-4 80-81 1.2254859589450589 3.601563366626185E-5 2.160938019975711E-4 82-83 1.3221339118884725 3.601563366626185E-5 2.160938019975711E-4 84-85 1.4281639374019475 3.601563366626185E-5 2.160938019975711E-4 86-87 1.5452327546341316 3.601563366626185E-5 2.160938019975711E-4 88-89 1.6686583312084111 3.601563366626185E-5 2.160938019975711E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 26830 0.0 73.40362 4 TCGGAAG 26905 0.0 73.393166 3 GATCGGA 27000 0.0 72.898155 1 ATCGGAA 27245 0.0 72.198364 2 GAAGAGC 27220 0.0 72.19489 6 GGAAGAG 29205 0.0 67.49936 5 GAGCACA 33370 0.0 58.946495 9 AGAGCAC 33425 0.0 58.86371 8 AAGAGCA 33965 0.0 57.690155 7 TCGCCGG 25 0.005026121 56.988255 2 CGGAGCG 35 2.7664844E-4 54.373432 1 CGATCGC 45 1.5021069E-5 52.863056 1 CGACGGG 110 0.0 47.576756 1 TCGGACG 95 3.8562575E-10 44.990726 3 GCCGTCT 22120 0.0 44.505253 50-51 CGTCTTC 22125 0.0 44.47894 52-53 ATGCCGT 22080 0.0 44.432087 48-49 CGCGGAG 75 1.3465433E-7 44.404972 1 TCGTATG 22400 0.0 44.122437 44-45 TCTCGTA 21680 0.0 44.04294 42-43 >>END_MODULE