FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776572
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1818236.548470560100729TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT54800.19736567249111497TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA268300.073.403624
TCGGAAG269050.073.3931663
GATCGGA270000.072.8981551
ATCGGAA272450.072.1983642
GAAGAGC272200.072.194896
GGAAGAG292050.067.499365
GAGCACA333700.058.9464959
AGAGCAC334250.058.863718
AAGAGCA339650.057.6901557
TCGCCGG250.00502612156.9882552
CGGAGCG352.7664844E-454.3734321
CGATCGC451.5021069E-552.8630561
CGACGGG1100.047.5767561
TCGGACG953.8562575E-1044.9907263
GCCGTCT221200.044.50525350-51
CGTCTTC221250.044.4789452-53
ATGCCGT220800.044.43208748-49
CGCGGAG751.3465433E-744.4049721
TCGTATG224000.044.12243744-45
TCTCGTA216800.044.0429442-43
Produced by FastQC (version 0.11.2)