FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339526
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1414856.047592546524381TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT44820.19157726821586937TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC30510.13041103197827253No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA29580.12643586777834484No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG27970.11955413190535176No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC26570.11357001375492301No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25670.1097230806582188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG206200.075.800913
GATCGGA206250.075.686521
CGGAAGA206550.075.4195254
GAAGAGC207800.075.122636
ATCGGAA208600.074.587282
GGAAGAG216050.072.297995
GAGCACA276900.056.187229
AGAGCAC277650.056.0867588
AAGAGCA278350.055.9457137
CGAGTGG502.8006043E-547.5780141
GCCGTCT174400.044.88551750-51
CGTCTTC174500.044.83353852-53
ATGCCGT174100.044.70271348-49
TCGTATG175800.044.53729244-45
CTGCTTG177000.044.37899858-59
TCTCGTA169050.044.3620342-43
TGCCGTC174700.044.3462250-51
GATCTCG169050.044.29084840-41
TATGCCG174050.044.2789148-49
GTATGCC176450.044.22660446-47
Produced by FastQC (version 0.11.2)