##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339526 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64635913428618 33.0 33.0 33.0 33.0 33.0 2 32.6526535717064 33.0 33.0 33.0 33.0 33.0 3 32.71451610283451 33.0 33.0 33.0 33.0 33.0 4 36.65185682911838 37.0 37.0 37.0 37.0 37.0 5 36.612913043069405 37.0 37.0 37.0 37.0 37.0 6 36.51763305900426 37.0 37.0 37.0 37.0 37.0 7 36.41612959206267 37.0 37.0 37.0 37.0 37.0 8 36.362678166432005 37.0 37.0 37.0 37.0 37.0 9 36.401657857189875 37.0 37.0 37.0 37.0 37.0 10-11 36.334221761160165 37.0 37.0 37.0 37.0 37.0 12-13 36.334229882463376 37.0 37.0 37.0 37.0 37.0 14-15 38.179913794503676 40.0 37.0 40.0 37.0 40.0 16-17 38.19952994751928 40.0 37.0 40.0 37.0 40.0 18-19 38.15674863198785 40.0 37.0 40.0 37.0 40.0 20-21 38.15208593535614 40.0 37.0 40.0 37.0 40.0 22-23 37.956687807701215 40.0 37.0 40.0 33.0 40.0 24-25 38.02554042998453 40.0 37.0 40.0 35.0 40.0 26-27 37.95989144809675 40.0 37.0 40.0 33.0 40.0 28-29 37.78466898850451 40.0 37.0 40.0 33.0 40.0 30-31 37.72054189609348 40.0 37.0 40.0 33.0 40.0 32-33 37.63402693537067 40.0 37.0 40.0 33.0 40.0 34-35 37.52603005908034 40.0 37.0 40.0 33.0 40.0 36-37 37.47564100591316 40.0 37.0 40.0 33.0 40.0 38-39 37.431640640027084 40.0 37.0 40.0 33.0 40.0 40-41 37.35878592501216 40.0 37.0 40.0 33.0 40.0 42-43 36.95875232846312 37.0 37.0 40.0 33.0 40.0 44-45 36.802031693599474 37.0 37.0 40.0 33.0 40.0 46-47 36.99067375186256 37.0 37.0 40.0 33.0 40.0 48-49 36.72613148988299 37.0 37.0 40.0 33.0 40.0 50-51 36.634212656751835 37.0 37.0 40.0 33.0 40.0 52-53 36.55751143607722 37.0 37.0 40.0 33.0 40.0 54-55 36.21401108600631 37.0 37.0 40.0 33.0 40.0 56-57 36.12004568446771 37.0 37.0 40.0 33.0 40.0 58-59 35.94089700221327 37.0 37.0 40.0 33.0 40.0 60-61 35.65312824050684 37.0 35.0 40.0 27.0 40.0 62-63 35.276306824544804 37.0 33.0 37.0 27.0 40.0 64-65 35.00747950653252 37.0 33.0 37.0 27.0 40.0 66-67 34.439207343709796 37.0 33.0 37.0 27.0 40.0 68-69 34.2090021653959 37.0 33.0 37.0 27.0 40.0 70-71 33.70450381829482 37.0 33.0 37.0 27.0 37.0 72-73 32.97488893049276 37.0 33.0 37.0 24.5 37.0 74-75 32.25230901473204 37.0 33.0 37.0 22.0 37.0 76-77 29.71388456465113 33.0 27.0 33.0 18.5 37.0 78-79 31.806253275236095 33.0 33.0 37.0 22.0 37.0 80-81 32.134500535578574 37.0 33.0 37.0 22.0 37.0 82-83 32.10635893766515 37.0 33.0 37.0 18.5 37.0 84-85 31.96914139872778 37.0 33.0 37.0 15.0 37.0 86-87 31.836914400609352 33.0 33.0 37.0 15.0 37.0 88-89 31.644852205104794 33.0 33.0 37.0 15.0 37.0 90-91 31.402804029534188 33.0 33.0 37.0 15.0 37.0 92-93 31.38637484687069 33.0 33.0 37.0 15.0 37.0 94-95 31.32728509963129 33.0 33.0 37.0 15.0 37.0 96-97 31.156587060797783 33.0 33.0 37.0 15.0 37.0 98-99 30.992290104918688 33.0 33.0 37.0 15.0 37.0 100-101 29.486392970199947 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16489083214856493 2306 2 0.12022117581025071 2306 3 0.1290195388434725 2306 4 0.10609847746010104 2306 5 0.1464495911274497 2306 6 0.20967350246193206 2306 7 0.19140111777549862 2306 8 0.16201987066418866 2306 9 0.1663282155988881 2306 10-11 0.07340820105012114 2306 12-13 0.19318014847885223 2306 14-15 0.36296298383803816 2306 16-17 0.403174814139021 2306 18-19 0.4091893850187418 2306 20-21 0.3420559547719151 2306 22-23 0.34868079001438446 2306 24-25 0.38878537890016673 2306 26-27 0.3494119889090044 2306 28-29 0.45978436983751436 2306 30-31 0.4766161037332992 2306 32-33 0.3718187700247242 2306 34-35 0.4540874596378828 2306 36-37 0.4946080169624594 2306 38-39 0.4837172878505527 2306 40-41 0.43327530395503544 2306 42-43 0.45652771602346576 2306 44-45 0.4238799534543105 2306 46-47 0.4044046975392561 2306 48-49 0.6544843780195109 2306 50-51 0.6241849638427936 2306 52-53 0.5566828071751146 2306 54-55 0.5465271178484912 2306 56-57 0.5911059219332984 2306 58-59 0.6766186994034555 2306 60-61 0.6359316908171238 2306 62-63 0.5148570579759166 2306 64-65 0.48864287286648533 2306 66-67 0.5669756087503188 2306 68-69 0.6049623519396121 2306 70-71 0.5345889902702083 2306 72-73 0.2054847667996711 2306 74-75 0.47748891753369804 2306 76-77 0.19896010121095387 2306 78-79 0.49515634399394415 2306 80-81 0.6378320809474545 2306 82-83 0.48921358632446754 2306 84-85 0.5556081787945217 2306 86-87 0.48838492333618433 2306 88-89 0.5747056168161429 2306 90-91 0.6329031928145774 2306 92-93 0.49314815463145223 2306 94-95 0.4861603168825859 2306 96-97 0.4218737700089754 2306 98-99 0.4215611644523882 2306 100-101 0.46476411125420825 2307 1 0.15244124866307374 2307 2 0.1101913666793024 2307 3 0.0568632649793841 2307 4 0.0985173729884039 2307 5 0.15783857872508378 2307 6 0.15914808991851714 2307 7 0.1483143415994448 2307 8 0.22879265150512396 2307 9 0.1066766565556847 2307 10-11 0.1621351257205177 2307 12-13 0.25791716382119745 2307 14-15 0.3718546856986933 2307 16-17 0.378852479234169 2307 18-19 0.31996518662017337 2307 20-21 0.32497301923027777 2307 22-23 0.33435387538771266 2307 24-25 0.31966801520420063 2307 26-27 0.37721892443099847 2307 28-29 0.3995329163626309 2307 30-31 0.4497226562289782 2307 32-33 0.28238728326361695 2307 34-35 0.4247429875913795 2307 36-37 0.3034574423684049 2307 38-39 0.3473452899837568 2307 40-41 0.31587176059314714 2307 42-43 0.3608288608447552 2307 44-45 0.3191582547978271 2307 46-47 0.46175506918449827 2307 48-49 0.6030285559594475 2307 50-51 0.49191820350744564 2307 52-53 0.24488167190629184 2307 54-55 0.41807288040492807 2307 56-57 0.4151215137299715 2307 58-59 0.39432674786881705 2307 60-61 0.2651209217568251 2307 62-63 0.23156414519096558 2307 64-65 0.20231227808671548 2307 66-67 0.26962679824981706 2307 68-69 0.2292885624135863 2307 70-71 0.03774894374154769 2307 72-73 -0.12419980008364462 2307 74-75 -0.017034657662435393 2307 76-77 -0.23018371460890208 2307 78-79 0.21104928876599516 2307 80-81 0.267945034682441 2307 82-83 0.09939779048726649 2307 84-85 0.2302452808731914 2307 86-87 0.04117222173153223 2307 88-89 0.3309069368022328 2307 90-91 0.44376559428095774 2307 92-93 0.1776735529826432 2307 94-95 0.08041320268047869 2307 96-97 0.07089104030917781 2307 98-99 -0.062021569941759225 2307 100-101 0.020883716775855987 2201 1 0.14145002357737013 2201 2 0.16198702355357852 2201 3 0.13396818102952324 2201 4 0.14988012819018337 2201 5 0.22692999803083325 2201 6 0.12331903375609699 2201 7 0.1982979548524284 2201 8 0.17902249548090765 2201 9 0.1749230976119449 2201 10-11 0.201514860663913 2201 12-13 0.20169552337114993 2201 14-15 0.3020610702044024 2201 16-17 0.40627275230843907 2201 18-19 0.39938012638749143 2201 20-21 0.36527947092294966 2201 22-23 0.4111621639839669 2201 24-25 0.3446306666970145 2201 26-27 0.45049501651852353 2201 28-29 0.34234694383457764 2201 30-31 0.4519099420396131 2201 32-33 0.29800857786088386 2201 34-35 0.4222560807193858 2201 36-37 0.35232339877783403 2201 38-39 0.38889395862238274 2201 40-41 0.43646207916244606 2201 42-43 0.3553918188829712 2201 44-45 0.4773011664949962 2201 46-47 0.41556774068241253 2201 48-49 0.5150107692453858 2201 50-51 0.49989356429571075 2201 52-53 0.3729062892516666 2201 54-55 0.5536693723134647 2201 56-57 0.5528976121951885 2201 58-59 0.5768782333619242 2201 60-61 0.46504587331895664 2201 62-63 0.5116629810941546 2201 64-65 0.47752242282373203 2201 66-67 0.4170204464395013 2201 68-69 0.36723998215145315 2201 70-71 0.34288136661905355 2201 72-73 0.13624352975540432 2201 74-75 0.3210907036855062 2201 76-77 0.12527606575401862 2201 78-79 0.5412985989997843 2201 80-81 0.5762013520402363 2201 82-83 0.5836792687254331 2201 84-85 0.5637567121585718 2201 86-87 0.531786481351233 2201 88-89 0.6026437159252787 2201 90-91 0.6258021792880832 2201 92-93 0.6148448906748456 2201 94-95 0.4545209449563892 2201 96-97 0.6837212775148238 2201 98-99 0.5220101633387486 2201 100-101 0.5194823180062578 1101 1 0.12889932675552274 1101 2 0.10186664517022592 1101 3 0.07956778290972011 1101 4 0.08906613176598199 1101 5 0.15209828517573243 1101 6 0.11253604071197287 1101 7 0.1352756999854492 1101 8 0.17550678448376544 1101 9 0.04581490042795622 1101 10-11 0.14264371779230345 1101 12-13 0.16807275036002522 1101 14-15 0.3067144444367713 1101 16-17 0.22078191033542538 1101 18-19 0.2355177260452237 1101 20-21 0.26898798380038613 1101 22-23 0.2808780324039759 1101 24-25 0.3154413645710079 1101 26-27 0.3229761321315934 1101 28-29 0.25914319640229877 1101 30-31 0.2989939663107677 1101 32-33 0.26188371863517546 1101 34-35 0.26671871747021925 1101 36-37 0.3552402346728343 1101 38-39 0.49188252489494033 1101 40-41 0.5678229143763005 1101 42-43 0.46714871947244063 1101 44-45 0.468505655914079 1101 46-47 0.45531626799070324 1101 48-49 0.5338526465218649 1101 50-51 0.37820249259601013 1101 52-53 0.46061070025850626 1101 54-55 0.5517143019082411 1101 56-57 0.4310266535736673 1101 58-59 0.5626618060832342 1101 60-61 0.45129044523421413 1101 62-63 0.5685318067529366 1101 64-65 0.5766763933132992 1101 66-67 0.4219410954884708 1101 68-69 0.497189770887168 1101 70-71 0.5361205603765953 1101 72-73 0.3277696215867536 1101 74-75 0.0764884956265206 1101 76-77 -0.10836986941238891 1101 78-79 0.3520364894186052 1101 80-81 0.42070986431929924 1101 82-83 0.4137576834666348 1101 84-85 0.44035341116499893 1101 86-87 0.4883351257418411 1101 88-89 0.6188106011240215 1101 90-91 0.6486415852698109 1101 92-93 0.5109788302632552 1101 94-95 0.3695410167466058 1101 96-97 0.30039508730042286 1101 98-99 0.249859451258299 1101 100-101 0.2597070283110199 2304 1 0.10987503846513391 2304 2 0.16188501814153966 2304 3 0.058783966678319644 2304 4 0.046672201651325906 2304 5 0.151787208412685 2304 6 0.05235726878470359 2304 7 0.13796845821561732 2304 8 0.1232458882910521 2304 9 0.14445053718750955 2304 10-11 0.08158032132011073 2304 12-13 0.1500521706879212 2304 14-15 0.3012900433810515 2304 16-17 0.15857870916110528 2304 18-19 0.25868808431815893 2304 20-21 0.28512752117091367 2304 22-23 0.32343236147458754 2304 24-25 0.22376850067195164 2304 26-27 0.31301195456509134 2304 28-29 0.20390033780126515 2304 30-31 0.10997594816137735 2304 32-33 0.3271490816957865 2304 34-35 0.46375911370424916 2304 36-37 0.44497615966445636 2304 38-39 0.2636736951754486 2304 40-41 0.27577530018925245 2304 42-43 0.45250623902410325 2304 44-45 0.4315312662807145 2304 46-47 0.35747290486204264 2304 48-49 0.48232569706697603 2304 50-51 0.37529586909123935 2304 52-53 0.3566916191014968 2304 54-55 0.5573209745048118 2304 56-57 0.5275892553419439 2304 58-59 0.4113642757411 2304 60-61 0.3145965107450266 2304 62-63 0.40458220118855337 2304 64-65 0.2396660354292024 2304 66-67 0.41041667353228917 2304 68-69 0.36034515859088856 2304 70-71 0.3972223553867309 2304 72-73 0.3018360267545148 2304 74-75 0.40073741536385654 2304 76-77 0.4722378968134251 2304 78-79 0.6816174592976232 2304 80-81 0.8503652009869782 2304 82-83 0.719507256951232 2304 84-85 0.9348731135034214 2304 86-87 0.7444387920731259 2304 88-89 0.853313874720893 2304 90-91 0.9513119848576288 2304 92-93 0.6510970394332105 2304 94-95 0.6237126047448704 2304 96-97 0.535338242882549 2304 98-99 0.6310986061016202 2304 100-101 0.6571902228749558 2202 1 0.13033264417821755 2202 2 0.1811084284129194 2202 3 0.11255244206166992 2202 4 0.1370074718225851 2202 5 0.18289285162440905 2202 6 0.15736052732187744 2202 7 0.13612630724151842 2202 8 0.1736872600894941 2202 9 0.12147882964872991 2202 10-11 0.20483595911183272 2202 12-13 0.15829450659543198 2202 14-15 0.25529112215928507 2202 16-17 0.2041182354129063 2202 18-19 0.264310203712526 2202 20-21 0.29583922748202696 2202 22-23 0.21624411989617442 2202 24-25 0.24197908706497628 2202 26-27 0.2806208543549502 2202 28-29 0.3573193203801779 2202 30-31 0.291095761765213 2202 32-33 0.24641572145829826 2202 34-35 0.292361364511045 2202 36-37 0.25057372575268744 2202 38-39 0.20101835959836478 2202 40-41 0.26419182090741344 2202 42-43 0.35248808524988107 2202 44-45 0.3585403601461721 2202 46-47 0.34273863562982143 2202 48-49 0.3861410588168468 2202 50-51 0.2893109745986209 2202 52-53 0.2307311995534107 2202 54-55 0.2890650036762281 2202 56-57 0.45136201449360414 2202 58-59 0.4214487626227168 2202 60-61 0.4979548544939263 2202 62-63 0.36718640581162276 2202 64-65 0.4169702913791369 2202 66-67 0.35199547723774316 2202 68-69 0.458991657589813 2202 70-71 0.4964088965180906 2202 72-73 0.3714854630079856 2202 74-75 0.20619556307811138 2202 76-77 0.07791277969546329 2202 78-79 0.5105540529965644 2202 80-81 0.57517259466659 2202 82-83 0.48576794514904975 2202 84-85 0.5300955364184432 2202 86-87 0.37966579914264287 2202 88-89 0.5343461845209241 2202 90-91 0.6457882092905258 2202 92-93 0.47643030485314597 2202 94-95 0.5268044275797088 2202 96-97 0.4043394979744228 2202 98-99 0.10510867034508564 2202 100-101 0.39459457272822007 1102 1 0.10137487526199607 1102 2 0.09214741251285119 1102 3 0.10880572709611869 1102 4 0.061659689411087015 1102 5 0.14058059324567296 1102 6 0.06097076749721708 1102 7 0.12268176471110337 1102 8 0.008391683090316349 1102 9 0.02949659673851812 1102 10-11 -0.0033737097972803554 1102 12-13 0.09789743598367551 1102 14-15 0.14845710898870834 1102 16-17 0.1602243407572388 1102 18-19 0.12643007829777986 1102 20-21 0.1401102035050812 1102 22-23 0.08591513447716181 1102 24-25 0.11517041558872165 1102 26-27 0.14624328593998115 1102 28-29 0.20125735372264586 1102 30-31 0.22660952085930575 1102 32-33 0.17540190148326218 1102 34-35 0.2280235209141992 1102 36-37 0.17716208430087477 1102 38-39 0.05172495909304331 1102 40-41 0.207127982160749 1102 42-43 0.21464220536798706 1102 44-45 0.05619352646277065 1102 46-47 0.1956229973438255 1102 48-49 0.1599949749837819 1102 50-51 0.05183405862447188 1102 52-53 -0.04292485353477815 1102 54-55 0.26036153955032404 1102 56-57 0.35114626763658663 1102 58-59 0.2714742565193973 1102 60-61 0.05334002795389381 1102 62-63 0.22436287431080615 1102 64-65 0.17453665161170306 1102 66-67 0.17879742644274188 1102 68-69 0.022174665717422215 1102 70-71 0.13287861332021578 1102 72-73 0.20339507002147172 1102 74-75 -0.12107060335010544 1102 76-77 -0.42398531067216183 1102 78-79 -0.16647442406653212 1102 80-81 0.011952170123457506 1102 82-83 -0.08561686809864 1102 84-85 0.025419118653381645 1102 86-87 -0.2455967499426137 1102 88-89 -0.12229859037177349 1102 90-91 -0.012960129166959433 1102 92-93 -0.049708549367423416 1102 94-95 -0.07595056212126394 1102 96-97 -0.18601734047798502 1102 98-99 -0.4578720354680321 1102 100-101 -0.23451996462731373 2305 1 0.11799617189848988 2305 2 0.18137745804988725 2305 3 0.1292445081731941 2305 4 0.11928519460498421 2305 5 0.11529584759618672 2305 6 0.02611656394999784 2305 7 0.04412759345176198 2305 8 0.13280437779176424 2305 9 0.020624389917983876 2305 10-11 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#Quality Count 7 2.0 8 31.0 9 97.0 10 59.0 11 31.0 12 37.0 13 75.0 14 218.0 15 454.0 16 900.0 17 1459.0 18 2079.0 19 2812.0 20 3857.0 21 5477.0 22 7565.0 23 10145.0 24 13441.0 25 18077.0 26 25104.0 27 36471.0 28 60372.0 29 87008.0 30 66776.0 31 52479.0 32 68734.0 33 95942.0 34 148833.0 35 264875.0 36 522542.0 37 710967.0 38 132555.0 39 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.71583797405478 24.388206251431825 20.356570126814272 14.539385647699124 2 29.895756661819533 27.46047703680147 28.86986509233067 13.773901209048327 3 34.94143685515784 18.143718000996785 30.390130308447095 16.52471483539828 4 29.854640774109264 17.555319778327608 28.93133353966216 23.65870590790097 5 36.41147822251174 16.249787350087157 30.233346412905863 17.105388014495244 6 32.83177874492525 17.10030151406738 33.06191083151032 17.00600890949705 7 31.79626984269463 24.18318924431701 25.54295186289872 18.477589050089634 8 29.097859993862002 30.69921001091674 22.011168074216744 18.191761921004513 9 33.25301791901437 23.36165530966529 24.186907946310495 19.198418825009853 10-11 25.919993913156475 29.319158340001504 27.999010033033095 16.761837713808927 12-13 22.381584987728285 28.432169593327878 27.662419652527902 21.523825766415932 14-15 22.251515905358605 28.586089660897123 27.974470042222226 21.187924391522042 16-17 25.2287223265647 25.451952068759464 28.532736230283334 20.786589374392502 18-19 20.86572237282253 25.490932778691068 32.02689348184205 21.616451366644355 20-21 24.008282019520195 25.852971926792005 31.735573787168853 18.403172266518943 22-23 20.47141173041035 32.46741006511576 28.333965940109234 18.727212264364663 24-25 20.763864133162016 24.71173647995363 32.1470246537119 22.377374733172445 26-27 20.471091152652292 24.914619457103704 28.94853487415827 25.665754516085737 28-29 23.407968109779503 29.193840974624774 28.233560986285255 19.16462992931047 30-31 19.343106253147006 26.915323873297414 31.16156862543951 22.580001248116073 32-33 19.078223537588386 30.50769685825248 28.24991045194625 22.164169152212885 34-35 19.65851202337568 30.37598214339144 31.905629601893715 18.05987623133917 36-37 19.209959624299966 31.056889301508082 32.23398671354796 17.49916436064399 38-39 19.563610281566092 27.215433761984826 31.479586068374076 21.741369888075006 40-41 23.1491993984695 26.44675950663556 28.02742281455745 22.376618280337492 42-43 19.963641189923916 30.065286419668126 31.34171318558274 18.629359204825217 44-45 19.481345222226427 26.86293983593464 31.567011591606647 22.088703350232283 46-47 22.974032980073115 26.64261299710347 28.369928937586135 22.01342508523728 48-49 19.68656750552029 30.80236658162071 31.016360071618998 18.49470584124 50-51 22.93464939896905 27.238899596776434 30.38671931271178 19.439731691542736 52-53 19.251023728004995 26.862122715701027 27.510033164961538 26.37682039133244 54-55 23.304976054708643 26.774713327707193 30.958234897280214 18.962075720303947 56-57 19.985143366513146 26.812739082244825 30.33057612742075 22.871541423821277 58-59 19.846670430570825 26.89616648285389 30.670959028100377 22.58620405847491 60-61 22.75842534497686 27.14992998920444 30.71834282843614 19.37330183738256 62-63 19.415078708247695 27.21064502118608 30.495085650578467 22.879190619987757 64-65 23.310580810288503 27.11885854482891 30.35156506335943 19.218995581523153 66-67 20.358664198073992 33.577841152859286 26.865013427835883 19.198481221230836 68-69 20.65045301345551 33.48829980678894 26.8789123743583 18.982334805397254 70-71 20.07455376554878 34.26401191355235 26.79811736456693 18.86331695633194 72-73 19.935506557830866 34.31455142641238 26.643059686895775 19.106882328860976 74-75 20.72104005519163 32.98747349290361 26.526658031038234 19.76482842086652 76-77 20.77709628939194 32.019039393544226 26.84118375173965 20.36268056532419 78-79 20.611080281722657 31.610516802206718 27.39423215869088 20.384170757379742 80-81 19.9071903041205 31.638120414499532 27.630879796247005 20.823809485132966 82-83 19.170205199270608 31.413214549203627 27.904104412857205 21.51247583866856 84-85 19.309820363325787 31.149797826987434 28.15582475859572 21.384557051091058 86-87 19.237974342042115 31.359415139995157 28.83949592209449 20.563114595868246 88-89 19.56262285199401 31.586475511903352 28.65760915456451 20.19329248153813 90-91 20.256307156450998 30.804292977065916 27.828400625131067 21.110999241352022 92-93 19.614623935479873 31.59358903910421 27.567516714810164 21.224270310605753 94-95 19.55883028981897 31.505264722138776 27.719625348923483 21.216279639118774 96-97 19.938898266040542 32.171217406083876 27.131222280539912 20.758662047335672 98-99 20.22588759499733 31.442633851495742 27.133493902745393 21.19798465076153 100-101 20.21352428820605 31.485339501223613 26.809177259710342 21.491958950859992 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 58.0 1 39.5 2 25.5 3 55.0 4 62.0 5 41.0 6 40.0 7 41.5 8 45.0 9 58.5 10 56.5 11 48.5 12 52.0 13 61.5 14 70.0 15 76.5 16 84.0 17 88.0 18 104.5 19 137.5 20 170.5 21 218.5 22 288.5 23 365.0 24 498.0 25 746.5 26 1150.5 27 1776.0 28 2486.5 29 3253.5 30 4300.5 31 5693.5 32 9837.0 33 18756.5 34 33555.5 35 55083.5 36 86200.0 37 125571.0 38 158565.0 39 175350.0 40 182644.5 41 174564.0 42 162615.5 43 162331.0 44 154704.0 45 139469.5 46 129220.0 47 113117.5 48 89493.0 49 72969.5 50 61208.0 51 49471.0 52 38599.0 53 30298.5 54 23451.0 55 16708.0 56 12014.5 57 9353.5 58 7471.0 59 5784.0 60 4372.0 61 3395.5 62 2612.0 63 1960.0 64 1535.0 65 1198.5 66 913.5 67 725.0 68 631.0 69 514.0 70 351.5 71 229.0 72 157.5 73 104.5 74 70.5 75 51.0 76 39.5 77 29.5 78 21.5 79 13.0 80 6.0 81 7.0 82 7.0 83 3.5 84 0.5 85 2.0 86 2.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18042116223542717 2 0.0 3 0.0 4 0.009318126834239071 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0023081598580225226 12-13 0.0 14-15 0.0 16-17 3.4194960859592927E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002457762811783242 40-41 0.006838992171918585 42-43 0.002799712420379171 44-45 0.0013464265838464713 46-47 0.007437403986961461 48-49 0.008655599467584459 50-51 0.00551393743860936 52-53 0.006817620321381339 54-55 0.018978203277074075 56-57 0.021521453491006298 58-59 0.02746282794036057 60-61 0.025688964345769186 62-63 0.010856900072920754 64-65 0.015879284949173466 66-67 0.023145714131836963 68-69 0.027056762780152902 70-71 0.028937485627430516 72-73 0.022590046017868577 74-75 0.03272030317252298 76-77 0.02953589744247339 78-79 0.020858926124351685 80-81 0.009531845339611529 82-83 0.001880722847277611 84-85 0.008249534307376793 86-87 0.027420084239286074 88-89 0.024940949576965594 90-91 0.02122224758348486 92-93 0.04406875580780038 94-95 0.04631280011421117 96-97 0.03434456381335365 98-99 0.04161099299601714 100-101 0.041162184134734985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2339526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.50029065499634 #Duplication Level Percentage of deduplicated Percentage of total 1 83.92063988507431 28.113658281032684 2 6.677787967514807 4.474156756883666 3 2.4863225806933484 2.498775873459233 4 1.5290416116576602 2.048933536564626 5 0.7935856206561226 1.3292674475802886 6 0.6034161433957802 1.2128769713805352 7 0.4758575657768376 1.1158956734732166 8 0.35394727144027016 0.9485869175833548 9 0.28543560758666436 0.8605958235694859 >10 2.2126887919353972 15.076426875710759 >50 0.3240706258297575 7.8243506991800675 >100 0.3113980062168871 21.141751735864236 >500 0.020126541923152833 4.321640642626205 >1k 0.005553202534095113 2.9387684928704303 >5k 0.0 0.0 >10k+ 1.285777648866904E-4 6.094314272221262 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 141485 6.047592546524381 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4482 0.19157726821586937 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3051 0.13041103197827253 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 2958 0.12643586777834484 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2797 0.11955413190535176 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2657 0.11357001375492301 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2567 0.1097230806582188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0036759582924062394 0.0 0.0 2 0.0040606516020766596 0.0 0.0 3 0.004616319716045045 0.0 0.0 4 0.004915525623566483 0.0 0.0 5 0.006625273666546129 0.0 0.0 6 0.008335021709525776 0.0 0.0 7 0.010002026051430931 0.0 0.0 8 0.011284337083665665 0.0 0.0 9 0.012951341425570822 0.0 0.0 10-11 0.019213293632983776 0.0 0.0 12-13 0.024919577726428346 0.0 0.0 14-15 0.03150210769189998 0.0 0.0 16-17 0.03690918587782312 0.0 0.0 18-19 0.04240175146589523 0.0 0.0 20-21 0.04887742217868064 0.0 0.0 22-23 0.05550269584522677 0.0 0.0 24-25 0.06321793388917242 0.0 0.0 26-27 0.07061259417505938 0.0 0.0 28-29 0.07864840997706374 0.0 0.0 30-31 0.13680121528890893 0.0 0.0 32-33 0.14879082344030373 0.0 0.0 34-35 0.15934851760570304 0.0 0.0 36-37 0.17080382949366668 0.0 0.0 38-39 0.18317813095473184 0.0 0.0 40-41 0.19779647672220785 0.0 0.0 42-43 0.21468023864663183 0.0 0.0 44-45 0.23464154704841922 0.0 0.0 46-47 0.25656906569963317 0.0 0.0 48-49 0.280484166450811 0.0 0.0 50-51 0.3066646833589368 0.0 0.0 52-53 0.3356021689863673 0.0 0.0 54-55 0.3666554678169851 0.0 0.0 56-57 0.3998245798507903 0.0 0.0 58-59 0.43780235825547564 0.0 0.0 60-61 0.4765495232795019 0.0 0.0 62-63 0.5213876657066432 0.0 0.0 64-65 0.5675081191660192 0.0 0.0 66-67 0.6137781755791558 0.0 4.274370107449116E-5 68-69 0.6644935769040395 0.0 4.274370107449116E-5 70-71 0.7191413987277765 0.0 4.274370107449116E-5 72-73 0.7788116054277662 0.0 4.274370107449116E-5 74-75 0.8425210918792951 0.0 4.274370107449116E-5 76-77 0.9080258137759529 0.0 4.274370107449116E-5 78-79 0.9784460612961771 0.0 4.274370107449116E-5 80-81 1.05275598561418 0.0 4.274370107449116E-5 82-83 1.1343537109653836 0.0 4.274370107449116E-5 84-85 1.2269365674927315 0.0 4.274370107449116E-5 86-87 1.3261874413877 0.0 4.274370107449116E-5 88-89 1.4324910259599593 0.0 4.274370107449116E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 20620 0.0 75.80091 3 GATCGGA 20625 0.0 75.68652 1 CGGAAGA 20655 0.0 75.419525 4 GAAGAGC 20780 0.0 75.12263 6 ATCGGAA 20860 0.0 74.58728 2 GGAAGAG 21605 0.0 72.29799 5 GAGCACA 27690 0.0 56.18722 9 AGAGCAC 27765 0.0 56.086758 8 AAGAGCA 27835 0.0 55.945713 7 CGAGTGG 50 2.8006043E-5 47.578014 1 GCCGTCT 17440 0.0 44.885517 50-51 CGTCTTC 17450 0.0 44.833538 52-53 ATGCCGT 17410 0.0 44.702713 48-49 TCGTATG 17580 0.0 44.537292 44-45 CTGCTTG 17700 0.0 44.378998 58-59 TCTCGTA 16905 0.0 44.36203 42-43 TGCCGTC 17470 0.0 44.34622 50-51 GATCTCG 16905 0.0 44.290848 40-41 TATGCCG 17405 0.0 44.27891 48-49 GTATGCC 17645 0.0 44.226604 46-47 >>END_MODULE