##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5394624 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.654197215598344 33.0 33.0 33.0 33.0 33.0 2 32.66205021888458 33.0 33.0 33.0 33.0 33.0 3 32.72760715111934 33.0 33.0 33.0 33.0 33.0 4 36.66249770141533 37.0 37.0 37.0 37.0 37.0 5 36.62796239367192 37.0 37.0 37.0 37.0 37.0 6 36.53408763984292 37.0 37.0 37.0 37.0 37.0 7 36.42737121252566 37.0 37.0 37.0 37.0 37.0 8 36.38333014497396 37.0 37.0 37.0 37.0 37.0 9 36.40948859457119 37.0 37.0 37.0 37.0 37.0 10-11 36.35391122717728 37.0 37.0 37.0 37.0 37.0 12-13 36.35995270476682 37.0 37.0 37.0 37.0 37.0 14-15 38.22295353670617 40.0 37.0 40.0 37.0 40.0 16-17 38.243695390077235 40.0 37.0 40.0 37.0 40.0 18-19 38.20880018700099 40.0 37.0 40.0 37.0 40.0 20-21 38.19977258841395 40.0 37.0 40.0 37.0 40.0 22-23 37.980329212934954 40.0 37.0 40.0 33.0 40.0 24-25 38.076246555830394 40.0 37.0 40.0 37.0 40.0 26-27 38.03587024786158 40.0 37.0 40.0 37.0 40.0 28-29 37.85234068213095 40.0 37.0 40.0 33.0 40.0 30-31 37.809540479558905 40.0 37.0 40.0 33.0 40.0 32-33 37.63981882703966 40.0 37.0 40.0 33.0 40.0 34-35 37.579781000492346 40.0 37.0 40.0 33.0 40.0 36-37 37.59529774456941 40.0 37.0 40.0 33.0 40.0 38-39 37.42776688421658 40.0 37.0 40.0 33.0 40.0 40-41 37.163024429506116 40.0 37.0 40.0 33.0 40.0 42-43 37.002009685939186 37.0 37.0 40.0 33.0 40.0 44-45 36.91703082550332 37.0 37.0 40.0 33.0 40.0 46-47 37.041601045781874 37.0 37.0 40.0 33.0 40.0 48-49 36.7510689345541 37.0 37.0 40.0 33.0 40.0 50-51 36.6747503625832 37.0 37.0 40.0 33.0 40.0 52-53 36.58428177756225 37.0 37.0 40.0 33.0 40.0 54-55 36.22772569506235 37.0 37.0 40.0 33.0 40.0 56-57 36.147208962107456 37.0 37.0 40.0 33.0 40.0 58-59 35.983331090359584 37.0 37.0 40.0 33.0 40.0 60-61 35.68383468430794 37.0 35.0 40.0 30.0 40.0 62-63 35.40521860281643 37.0 33.0 40.0 27.0 40.0 64-65 35.08301477545052 37.0 33.0 37.0 27.0 40.0 66-67 34.38761746879857 37.0 33.0 37.0 27.0 40.0 68-69 34.17668979339431 37.0 33.0 37.0 27.0 40.0 70-71 33.62976400208801 37.0 33.0 37.0 27.0 37.0 72-73 32.76402859587619 37.0 33.0 37.0 22.0 37.0 74-75 31.924270254979774 35.0 33.0 37.0 18.5 37.0 76-77 29.405224441962964 33.0 27.0 33.0 15.0 37.0 78-79 31.418014675350868 33.0 33.0 37.0 15.0 37.0 80-81 31.715613914890085 35.0 33.0 37.0 15.0 37.0 82-83 31.672099297374572 37.0 33.0 37.0 15.0 37.0 84-85 31.530006725213845 33.0 33.0 37.0 15.0 37.0 86-87 31.395688652258247 33.0 33.0 37.0 15.0 37.0 88-89 31.205689219489624 33.0 33.0 37.0 15.0 37.0 90-91 30.961848036118923 33.0 33.0 37.0 15.0 37.0 92-93 30.936675660806017 33.0 33.0 37.0 15.0 37.0 94-95 30.86967581058476 33.0 33.0 37.0 15.0 37.0 96-97 30.705445272923562 33.0 33.0 37.0 15.0 37.0 98-99 30.544065907095657 33.0 33.0 37.0 15.0 37.0 100-101 29.06003133860673 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15579374908962507 2306 2 0.16485015069824271 2306 3 0.1037499914034754 2306 4 0.1457469509569549 2306 5 0.1784538738021979 2306 6 0.1758634774332961 2306 7 0.16195842851745113 2306 8 0.15136593476525206 2306 9 0.222345762807322 2306 10-11 0.21939343298829073 2306 12-13 0.23484882033244503 2306 14-15 0.42801631687244424 2306 16-17 0.4374285855071349 2306 18-19 0.43545592537017797 2306 20-21 0.4160294146737513 2306 22-23 0.40590695033834123 2306 24-25 0.45917412195309737 2306 26-27 0.4180050392081682 2306 28-29 0.5030019903788556 2306 30-31 0.48805041525157833 2306 32-33 0.5327984732381168 2306 34-35 0.46998201280622 2306 36-37 0.4971378023545938 2306 38-39 0.45780441293945984 2306 40-41 0.397236001892729 2306 42-43 0.47434465651835467 2306 44-45 0.6221493057085326 2306 46-47 0.5529397445941342 2306 48-49 0.6692409961388321 2306 50-51 0.5604980289146937 2306 52-53 0.49092989137159293 2306 54-55 0.5624980688013537 2306 56-57 0.5291019560385806 2306 58-59 0.5585695284336438 2306 60-61 0.5099530019016996 2306 62-63 0.5508701624421519 2306 64-65 0.5805738013677413 2306 66-67 0.5251888646014535 2306 68-69 0.5481353474367623 2306 70-71 0.45484734005542293 2306 72-73 0.29238432860849173 2306 74-75 0.28217303531515014 2306 76-77 0.18177317278626504 2306 78-79 0.6588648058809845 2306 80-81 0.7207904340951536 2306 82-83 0.7183779268026278 2306 84-85 0.7260842167825245 2306 86-87 0.7476421967511122 2306 88-89 0.836074316641966 2306 90-91 0.8224053659628083 2306 92-93 0.7333699831987879 2306 94-95 0.6129040001566146 2306 96-97 0.6651862018025518 2306 98-99 0.6475418901013335 2306 100-101 0.7137280125864187 2307 1 0.16583447391014516 2307 2 0.13399354571006228 2307 3 0.1389213675146408 2307 4 0.09322678289094455 2307 5 0.18908815423325365 2307 6 0.15604615181796078 2307 7 0.1267462762867808 2307 8 0.19405787420771503 2307 9 0.23328139191510644 2307 10-11 0.1822161677531824 2307 12-13 0.1976519562528054 2307 14-15 0.3164540706133536 2307 16-17 0.32946894229029056 2307 18-19 0.32752380603399445 2307 20-21 0.2400019649793208 2307 22-23 0.3762890843999642 2307 24-25 0.3891159475841519 2307 26-27 0.39910573530914206 2307 28-29 0.4303965252476374 2307 30-31 0.3535309177554282 2307 32-33 0.38794860893709426 2307 34-35 0.396906898853409 2307 36-37 0.3914966432552518 2307 38-39 0.3073622682681574 2307 40-41 0.3161598898457427 2307 42-43 0.3397316041351175 2307 44-45 0.26994205489896217 2307 46-47 0.41207206297038823 2307 48-49 0.48624602567586805 2307 50-51 0.33339987066678134 2307 52-53 0.30474221651931543 2307 54-55 0.3909453378470644 2307 56-57 0.40044534107997976 2307 58-59 0.4426590680567841 2307 60-61 0.44979888658498623 2307 62-63 0.24871572986093327 2307 64-65 0.28849110318824245 2307 66-67 0.2556253703300442 2307 68-69 0.22641962380993164 2307 70-71 0.30291893871212494 2307 72-73 0.1479164444721235 2307 74-75 0.15885585590192974 2307 76-77 -0.09947757943449176 2307 78-79 0.37499987690837955 2307 80-81 0.549856020378801 2307 82-83 0.46179241908272317 2307 84-85 0.42756951324267334 2307 86-87 0.38541908140033243 2307 88-89 0.6447943950268176 2307 90-91 0.7599689060087051 2307 92-93 0.4502017668422731 2307 94-95 0.5248775985399838 2307 96-97 0.43762122275424176 2307 98-99 0.22486952582694641 2307 100-101 0.2884485416131639 2201 1 0.15177261026038025 2201 2 0.14667948654651042 2201 3 0.10403053005803997 2201 4 0.0782654504846434 2201 5 0.11937454418530535 2201 6 0.09927667907878401 2201 7 0.1041689917261408 2201 8 0.13935847340354002 2201 9 0.17966090360313558 2201 10-11 0.17889139596736214 2201 12-13 0.17277481052199306 2201 14-15 0.3023910819245046 2201 16-17 0.3285123777996688 2201 18-19 0.22608831240059146 2201 20-21 0.23949569738650922 2201 22-23 0.26568480340120004 2201 24-25 0.22743151655017613 2201 26-27 0.2578458265273085 2201 28-29 0.3247539479180759 2201 30-31 0.2800650210655107 2201 32-33 0.2345320902130723 2201 34-35 0.2418942309131964 2201 36-37 0.334245372523597 2201 38-39 0.21738164144137073 2201 40-41 0.21649070759166023 2201 42-43 0.29480176157308335 2201 44-45 0.1725822966473629 2201 46-47 0.2584971949937298 2201 48-49 0.3412743316595055 2201 50-51 0.2806628193042471 2201 52-53 0.19655817587390345 2201 54-55 0.39583025759802837 2201 56-57 0.36863289184736914 2201 58-59 0.5098312147888819 2201 60-61 0.35322778073906136 2201 62-63 0.253628775589263 2201 64-65 0.3068418971283293 2201 66-67 0.27344598959655997 2201 68-69 0.28996380072008066 2201 70-71 0.28443117126220585 2201 72-73 0.05426143996071886 2201 74-75 0.2980805689607102 2201 76-77 0.044208194896167896 2201 78-79 0.5318981103719942 2201 80-81 0.5936071032289298 2201 82-83 0.39562971367870503 2201 84-85 0.3820529627925424 2201 86-87 0.4295242703056452 2201 88-89 0.45454944026463906 2201 90-91 0.45623788260564524 2201 92-93 0.4690242334229886 2201 94-95 0.3870679034030786 2201 96-97 0.3768376105237188 2201 98-99 0.37753564347453406 2201 100-101 0.3936118522882097 1101 1 0.10230068840839124 1101 2 0.11267016283061793 1101 3 0.09978268649953037 1101 4 0.09408576544329605 1101 5 0.15388704923810081 1101 6 0.1336335790621419 1101 7 0.11028129872340031 1101 8 0.11316576928815181 1101 9 0.057488894612312436 1101 10-11 0.1505403271528607 1101 12-13 0.15881563709611868 1101 14-15 0.28680714680167085 1101 16-17 0.24056596608754433 1101 18-19 0.2189638731033554 1101 20-21 0.21988631156846594 1101 22-23 0.16437121687568634 1101 24-25 0.2310786196383603 1101 26-27 0.15585562020547883 1101 28-29 0.169956451306426 1101 30-31 0.2786053056444615 1101 32-33 0.15940771105981355 1101 34-35 0.16900164806485662 1101 36-37 0.20752147815880306 1101 38-39 0.2971867609530605 1101 40-41 0.3276547943612371 1101 42-43 0.22148921987442094 1101 44-45 0.2636560205808891 1101 46-47 0.3826055254947036 1101 48-49 0.3601422330961057 1101 50-51 0.1946518549164793 1101 52-53 0.30841007201783555 1101 54-55 0.45590675235080624 1101 56-57 0.21554075686189123 1101 58-59 0.4154310391125122 1101 60-61 0.31933489856951525 1101 62-63 0.24080621851892658 1101 64-65 0.24968570740343665 1101 66-67 0.24172703263307227 1101 68-69 0.3360807723726822 1101 70-71 0.3399307789276307 1101 72-73 -0.002965558812626057 1101 74-75 0.0700817270820373 1101 76-77 -0.03423441865357191 1101 78-79 0.23728264022088652 1101 80-81 0.37575056480881486 1101 82-83 0.31248922572918403 1101 84-85 0.29290681851132305 1101 86-87 0.2432368534057474 1101 88-89 0.3757249823647264 1101 90-91 0.3761514501088712 1101 92-93 0.2667110679053515 1101 94-95 0.20693895050980515 1101 96-97 0.1670984116135834 1101 98-99 0.28196266015120486 1101 100-101 0.36623112969323657 2304 1 0.13901653608925102 2304 2 0.14595776347741207 2304 3 0.1116552349457649 2304 4 0.14077518446772785 2304 5 0.17058512583400898 2304 6 0.12748661809130368 2304 7 0.16527045998081746 2304 8 0.12516966737915425 2304 9 0.1257776601482945 2304 10-11 0.09593253793782708 2304 12-13 0.13143509523036556 2304 14-15 0.2330040472696382 2304 16-17 0.21335488443865813 2304 18-19 0.24637964927162415 2304 20-21 0.2647374481050875 2304 22-23 0.32523772611453694 2304 24-25 0.20522582967282688 2304 26-27 0.29862682499705073 2304 28-29 0.38222578646948335 2304 30-31 0.20528607775027297 2304 32-33 0.24106697559341228 2304 34-35 0.34911503487337114 2304 36-37 0.2888642731407671 2304 38-39 0.320714513066342 2304 40-41 0.27882114298705574 2304 42-43 0.2671016623610143 2304 44-45 0.35536302692860744 2304 46-47 0.23496804540088334 2304 48-49 0.23500604972049643 2304 50-51 0.3460515755348226 2304 52-53 0.3623472376714716 2304 54-55 0.4630641628090828 2304 56-57 0.41188770272697894 2304 58-59 0.31348669684665253 2304 60-61 0.3203598891976682 2304 62-63 0.3762307915979548 2304 64-65 0.25936195769386217 2304 66-67 0.2992727181807524 2304 68-69 0.3182888078390036 2304 70-71 0.3750644120108646 2304 72-73 0.08618404438468019 2304 74-75 0.20899383030730334 2304 76-77 0.35230289790834135 2304 78-79 0.5431022976912736 2304 80-81 0.6093355078144675 2304 82-83 0.4865007397559502 2304 84-85 0.5539138761312046 2304 86-87 0.4280703584097658 2304 88-89 0.529767834946643 2304 90-91 0.5882566781123586 2304 92-93 0.4333959775782361 2304 94-95 0.3419600735141124 2304 96-97 0.43315167117805586 2304 98-99 0.4102023384180775 2304 100-101 0.35939498256927394 2202 1 0.12054237090183761 2202 2 0.13912221479094455 2202 3 0.08250645029539783 2202 4 0.05444210139094707 2202 5 0.06605769878346734 2202 6 0.06846125938549363 2202 7 0.11812178338652046 2202 8 0.14255848025121765 2202 9 0.13670486678401517 2202 10-11 0.13908045080551545 2202 12-13 0.08224865719599705 2202 14-15 0.09966955677479206 2202 16-17 0.188545968883993 2202 18-19 0.11451222771189862 2202 20-21 0.13628071123702057 2202 22-23 0.09183561501186688 2202 24-25 0.17266125089225426 2202 26-27 0.06874230564242367 2202 28-29 0.12996586224075202 2202 30-31 0.11642805193464767 2202 32-33 0.1929667210749244 2202 34-35 0.29930387629962496 2202 36-37 0.2485278960651769 2202 38-39 0.2201994621027481 2202 40-41 0.1734367970844275 2202 42-43 0.3636557005409884 2202 44-45 0.3022623922581076 2202 46-47 0.314462621695192 2202 48-49 0.3599209677897903 2202 50-51 0.3379213342348706 2202 52-53 0.34073810189755704 2202 54-55 0.47140907386940256 2202 56-57 0.3966925747355461 2202 58-59 0.3525815536955861 2202 60-61 0.3878530788539152 2202 62-63 0.29447305183754935 2202 64-65 0.25123479672527793 2202 66-67 0.23921657302967247 2202 68-69 0.2223805880236185 2202 70-71 0.33870364262231334 2202 72-73 0.19853116154899197 2202 74-75 0.26477997610134807 2202 76-77 0.10926878617200941 2202 78-79 0.5403832696287161 2202 80-81 0.6581304992934385 2202 82-83 0.5072222755993927 2202 84-85 0.4662608466130749 2202 86-87 0.4740854730579862 2202 88-89 0.6379694128097206 2202 90-91 0.617043817191032 2202 92-93 0.45771106222989744 2202 94-95 0.5180912946528125 2202 96-97 0.3569292624518212 2202 98-99 0.18423807857403673 2202 100-101 0.32374210266723935 1102 1 0.08474111838655318 1102 2 0.13259193229244204 1102 3 0.08742340237596835 1102 4 0.0945059289389576 1102 5 0.17814411893124316 1102 6 0.1112730411593148 1102 7 0.10544158875823939 1102 8 0.12324425302262654 1102 9 0.12151334096911626 1102 10-11 0.1133749746156596 1102 12-13 0.10261114315670739 1102 14-15 0.20267893978837748 1102 16-17 0.13998569406523131 1102 18-19 0.1988164894701825 1102 20-21 0.19933361048764908 1102 22-23 0.13106085424266212 1102 24-25 0.14056422237824506 1102 26-27 0.17315665545294934 1102 28-29 0.25882453878055145 1102 30-31 0.27776541081549055 1102 32-33 0.2081178996280144 1102 34-35 0.18683419216804253 1102 36-37 0.15411065964025283 1102 38-39 0.12733976066315478 1102 40-41 0.15589255219311582 1102 42-43 0.22008131118128205 1102 44-45 0.19093405258877283 1102 46-47 0.24379767128451846 1102 48-49 0.2209125630047808 1102 50-51 0.22761031028483814 1102 52-53 0.17909562997553508 1102 54-55 0.28228687300444477 1102 56-57 0.3978138739127175 1102 58-59 0.2835254532666127 1102 60-61 0.19836495236000218 1102 62-63 0.1728783292313949 1102 64-65 0.1524520752476306 1102 66-67 0.1263584444098811 1102 68-69 0.036025313231697 1102 70-71 -0.028074482674483647 1102 72-73 0.05990420057055701 1102 74-75 0.010846351430753742 1102 76-77 -0.19533460537821412 1102 78-79 0.1379465890439171 1102 80-81 0.1805300669313432 1102 82-83 0.13122408924265017 1102 84-85 0.07290152986093545 1102 86-87 0.034721363639199865 1102 88-89 0.06034466474675071 1102 90-91 0.20615393021236983 1102 92-93 0.0864452433146603 1102 94-95 0.07570692782829624 1102 96-97 0.07749231794132427 1102 98-99 -0.023598064405085495 1102 100-101 0.02705216495697016 2305 1 0.11811798017781427 2305 2 0.0914679569219814 2305 3 0.10192622781922012 2305 4 0.08531026156067156 2305 5 0.13347590151274602 2305 6 0.08316490091559814 2305 7 0.05925476968180021 2305 8 0.11485480955825977 2305 9 0.03640344126687012 2305 10-11 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-1.4689321889221212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 24.0 10 59.0 11 39.0 12 44.0 13 139.0 14 383.0 15 963.0 16 1780.0 17 3000.0 18 4583.0 19 6532.0 20 9135.0 21 12826.0 22 17398.0 23 23530.0 24 31894.0 25 43381.0 26 59573.0 27 87805.0 28 143407.0 29 225684.0 30 197235.0 31 126472.0 32 149978.0 33 215273.0 34 336660.0 35 598396.0 36 1188841.0 37 1632496.0 38 277058.0 39 30.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.07180354360311 24.71487775885887 20.627260573919525 14.586058123618493 2 28.07281842070921 29.39726661209382 28.642070327793 13.887844639403971 3 32.98643241864493 18.189868283683904 31.813079095039804 17.01062020263136 4 27.32559443707121 17.82765250493267 29.065743046570745 25.78101001142537 5 36.817857926706296 16.945889092548434 29.152893695649595 17.083359285095682 6 32.718091195975845 17.726110290541104 32.6007150822745 16.95508343120855 7 29.74368556548149 26.670199813740492 25.44551761160741 18.140597009170612 8 26.831564164620186 32.52640035709625 22.722677243122043 17.91935823516152 9 34.452428936659906 23.003790440260527 23.810130233358247 18.733650389721323 10-11 26.178040024280286 29.94473723748844 26.90093364529302 16.976289092938263 12-13 21.760932735997912 28.77201450925959 26.506277360572305 22.960775394170195 14-15 21.356817453820693 29.14995558541244 26.931617476954834 22.56160948381203 16-17 25.238038744263946 25.25588991223639 27.618166668365895 21.88790467513377 18-19 20.081204918081408 25.388210559253068 32.07114156612212 22.459442956543406 20-21 24.348805403305214 25.840308796312772 31.706028075357985 18.104857725024022 22-23 19.78367723125838 34.64074604643438 27.296091442146846 18.279485280160397 24-25 20.032591706113344 25.19232109596517 31.781751610492226 22.99333558742926 26-27 20.104023931973757 25.232703891874575 27.05378539820384 27.609486777947822 28-29 24.043232670154584 29.984184254546747 26.917622062260506 19.054961013038167 30-31 19.07758538871291 26.4763030750614 31.045778167301375 23.400333368924322 32-33 18.745523320995126 30.801979897023408 27.335009817180953 23.117486964800513 34-35 19.13374314873474 30.768270411431825 32.241264636793964 17.85672180303947 36-37 18.804702607633082 27.054981032969117 32.27365799729508 21.866658362102715 38-39 23.91563327550588 31.0002147559091 26.942254944460526 18.141897024124496 40-41 19.43182408032212 34.78349288505602 26.869721260297947 18.914961774323913 42-43 19.561083932048202 30.748186430143008 31.342517181017378 18.34821245679141 44-45 19.1810442108034 26.514025028025955 31.46653962492718 22.83839113624346 46-47 23.907234150154018 26.280577014627433 27.033184236245724 22.779004598972833 48-49 19.941034334194267 31.001151987447155 30.741092767802787 18.316720910555794 50-51 24.101384226955417 26.43931972399438 30.473607331279872 18.985688717770323 52-53 19.326666625882627 26.255133459281865 26.65004346280968 27.768156452025828 54-55 23.927751111941724 26.503542018414212 30.812348066613325 18.756358803030736 56-57 19.696587499335998 26.064348984819457 30.765904084204365 23.473159431640177 58-59 19.555421310654843 26.006843708619222 31.30477050565363 23.132964475072306 60-61 23.671820678223174 26.57792854348338 30.7582589960339 18.991991782259547 62-63 19.26401703802791 26.7069011113446 30.65564909789979 23.373432752727705 64-65 24.02217707546932 26.399745039089556 30.733728258241733 18.84434962719939 66-67 20.000930773015334 34.80392070518479 26.238398595756472 18.956749926043408 68-69 19.92346769673924 34.86645785230239 26.278161314890795 18.931913136067575 70-71 19.574063741821167 35.40510662174207 26.42677956780196 18.594050068634797 72-73 19.510637211043903 35.631651009108076 26.094107772247256 18.76360400760077 74-75 20.43653569594462 34.430527551637155 25.7872349973247 19.34570175509353 76-77 20.843984482112187 33.05558759295843 26.37481870023412 19.725609224695262 78-79 20.564057205572897 32.43607044819079 27.14513250265992 19.854739843576397 80-81 19.959592929726718 32.59829641819204 26.991639299602134 20.45047135247911 82-83 19.147593398605046 32.41521753577445 27.357702293241122 21.079486772379376 84-85 19.362026234459474 31.821475758327967 27.64249880844306 21.173999198769494 86-87 19.25090373866711 31.87007338194807 28.106587761665164 20.772435117719656 88-89 19.588783036604948 32.28220964685552 27.727737954409765 20.40126936212977 90-91 20.010112222681006 31.321395323569806 27.518734218192993 21.1497582355562 92-93 19.653068239593612 31.90605315443889 27.013677554365785 21.42720105160172 94-95 19.359124876937212 32.13259040206214 26.924427554603618 21.583857166397028 96-97 19.98176060591282 32.456784819097884 26.630822684569562 20.930631890419736 98-99 20.338394175230263 31.400360566324466 26.797618218622844 21.46362703982243 100-101 20.23784609778385 31.505145481494395 26.403513123446587 21.85349529727517 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45.0 1 33.0 2 29.0 3 67.5 4 85.0 5 57.0 6 45.5 7 51.5 8 59.5 9 63.5 10 67.5 11 71.0 12 70.5 13 75.5 14 84.0 15 92.5 16 108.0 17 134.5 18 167.0 19 204.5 20 242.0 21 308.0 22 413.0 23 516.5 24 708.5 25 1009.0 26 1408.5 27 1988.5 28 2674.5 29 3520.0 30 5047.5 31 8343.0 32 18605.5 33 39909.5 34 75048.0 35 124626.0 36 194674.0 37 290003.5 38 374148.0 39 401812.5 40 407208.5 41 387420.0 42 355767.5 43 367307.5 44 363437.0 45 337577.5 46 337199.5 47 308647.0 48 238337.5 49 185259.0 50 148808.5 51 118810.5 52 90348.0 53 62716.5 54 44340.5 55 31027.5 56 21910.5 57 15080.0 58 9048.0 59 5714.5 60 3935.0 61 2808.0 62 1851.0 63 1040.0 64 656.5 65 460.0 66 349.5 67 278.5 68 221.0 69 148.0 70 97.0 71 70.5 72 49.0 73 29.5 74 14.5 75 17.0 76 14.5 77 5.0 78 3.5 79 5.5 80 4.5 81 2.0 82 1.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17893740138330308 2 0.0 3 0.0 4 0.009027505902172236 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002289316178476943 12-13 0.0 14-15 0.0 16-17 3.522024889964528E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0023634640708972486 40-41 0.006376718748146303 42-43 0.0028361568850766984 44-45 0.001325393577012967 46-47 0.00696990188750875 48-49 0.008675303413175784 50-51 0.005811341068441471 52-53 0.006617699398512296 54-55 0.018879907107520376 56-57 0.021771674911912304 58-59 0.027722043278641847 60-61 0.026211279970578115 62-63 0.010612417102656275 64-65 0.01586764897794545 66-67 0.02331024367963365 68-69 0.027369840789645394 70-71 0.029418176317756343 72-73 0.022661449620955974 74-75 0.03294946969427341 76-77 0.03006697037643402 78-79 0.020918974149078784 80-81 0.009611420554982144 82-83 0.001807354877744955 84-85 0.00780406567723719 86-87 0.027286424410672548 88-89 0.025284431315324294 90-91 0.021336056043943005 92-93 0.04575851810988124 94-95 0.047445382662443206 96-97 0.03523878587275035 98-99 0.042143808354391334 100-101 0.04210673440818118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5394624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.483157780264783 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22286355290572 17.019572775114618 2 8.457312103242325 3.633795406217957 3 2.864505311231377 1.8461585869077042 4 1.7992284808948595 1.5461243735124155 5 1.159493839949116 1.2454794554435968 6 0.6827462338577623 0.880052703950872 7 0.671233488238191 1.0094150454652993 8 0.6146477762922719 1.0563660125900614 9 0.3757010296031557 0.7264120047455273 >10 3.1761043974420664 13.961105907085802 >50 0.45190311452350834 6.748504632972868 >100 0.4211179851309082 19.58579658213278 >500 0.06740506446573637 10.038273594763917 >1k 0.03522121922328526 12.31302387261727 >5k 4.303360879651426E-4 0.6551726361970309 >10k+ 8.606691139909966E-5 7.734746410282464 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 418323 7.754442200234902 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8799 0.16310682635156779 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7541 0.1397873141853816 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7173 0.13296570808271346 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6451 0.11958201350084825 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 5470 0.10139724288476823 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005283037334946792 0.0 0.0 2 0.005635239823943245 0.0 0.0 3 0.005987442312939697 0.0 0.0 4 0.006358181775041227 0.0 0.0 5 0.007081123726139209 0.0 0.0 6 0.00785967659655242 0.0 0.0 7 0.008842136171121472 0.0 0.0 8 0.009917280611215907 0.0 0.0 9 0.011251942674781412 0.0 0.0 10-11 0.01712816314909065 0.0 0.0 12-13 0.02241120048403744 0.0 0.0 14-15 0.028185467606268756 0.0 0.0 16-17 0.032411897474226195 0.0 0.0 18-19 0.03763932388985775 0.0 0.0 20-21 0.04367310863556015 0.0 0.0 22-23 0.05041129835925544 0.0 0.0 24-25 0.05777974516852333 0.0 0.0 26-27 0.06550966295334021 0.0 0.0 28-29 0.0742313087992787 0.0 0.0 30-31 0.11531850968668067 0.0 0.0 32-33 0.12668167420009255 0.0 0.0 34-35 0.13732189676240641 0.0 0.0 36-37 0.14857383943718783 0.0 0.0 38-39 0.16048384465719945 0.0 0.0 40-41 0.1730704493955464 0.0 0.0 42-43 0.1874458720385332 0.0 0.0 44-45 0.2025535051191705 0.0 0.0 46-47 0.21907921664234617 0.0 0.0 48-49 0.23617030584522666 0.0 0.0 50-51 0.254568251652015 0.0 0.0 52-53 0.2739857309795826 0.0 0.0 54-55 0.29514568577902744 0.0 0.0 56-57 0.3166949170136788 0.0 0.0 58-59 0.3408856669158036 0.0 0.0 60-61 0.3665501061797819 0.0 0.0 62-63 0.3942350755122136 0.0 0.0 64-65 0.42441326772727817 0.0 0.0 66-67 0.4566397954704535 0.0 0.0 68-69 0.49161906371973285 0.0 0.0 70-71 0.5291935082037229 0.0 1.853697310507646E-5 72-73 0.5687884827561661 0.0 1.853697310507646E-5 74-75 0.6117015754944182 0.0 1.853697310507646E-5 76-77 0.6584703586385261 0.0 1.853697310507646E-5 78-79 0.7103646148461875 0.0 1.853697310507646E-5 80-81 0.7649930745868478 0.0 1.853697310507646E-5 82-83 0.8253401905304243 0.0 1.853697310507646E-5 84-85 0.890340086723375 0.0 1.853697310507646E-5 86-87 0.9610679076057942 0.0 1.853697310507646E-5 88-89 1.0375699956104447 0.0 5.561091931522938E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 55060 0.0 82.13252 3 GATCGGA 54990 0.0 82.119064 1 GAAGAGC 54990 0.0 81.85783 6 CGGAAGA 55025 0.0 81.822266 4 ATCGGAA 55585 0.0 81.01502 2 GGAAGAG 56200 0.0 80.213715 5 ACGCGGC 35 3.4220157E-6 67.846146 2 GTGCTCG 40 7.484534E-6 59.46128 1 CTCGGAA 340 0.0 58.66696 2 ATCGGAC 190 0.0 57.490685 2 GGTACGG 50 4.2025567E-7 57.08283 1 AAGAGCA 79730 0.0 56.505226 7 TCGGACG 185 0.0 56.477333 3 GAGCACA 79965 0.0 56.41044 9 AGAGCAC 80235 0.0 56.297558 8 CGGGTCG 55 8.114748E-7 51.893482 1 CGACCGC 75 2.3574103E-9 50.74029 1 CACGCCG 110 0.0 47.569027 1 CGGCGCC 60 1.4784528E-6 47.569023 1 ACGAGCG 80 4.1763997E-9 47.569023 1 >>END_MODULE