FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394624
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4183237.754442200234902TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC87990.16310682635156779No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG75410.1397873141853816No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC71730.13296570808271346No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG64510.11958201350084825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT54700.10139724288476823TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG550600.082.132523
GATCGGA549900.082.1190641
GAAGAGC549900.081.857836
CGGAAGA550250.081.8222664
ATCGGAA555850.081.015022
GGAAGAG562000.080.2137155
ACGCGGC353.4220157E-667.8461462
GTGCTCG407.484534E-659.461281
CTCGGAA3400.058.666962
ATCGGAC1900.057.4906852
GGTACGG504.2025567E-757.082831
AAGAGCA797300.056.5052267
TCGGACG1850.056.4773333
GAGCACA799650.056.410449
AGAGCAC802350.056.2975588
CGGGTCG558.114748E-751.8934821
CGACCGC752.3574103E-950.740291
CACGCCG1100.047.5690271
CGGCGCC601.4784528E-647.5690231
ACGAGCG804.1763997E-947.5690231
Produced by FastQC (version 0.11.2)