##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2881122 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64438090438378 33.0 33.0 33.0 33.0 33.0 2 32.6473290613865 33.0 33.0 33.0 33.0 33.0 3 32.71395484120423 33.0 33.0 33.0 33.0 33.0 4 36.646934423464195 37.0 37.0 37.0 37.0 37.0 5 36.61237392932337 37.0 37.0 37.0 37.0 37.0 6 36.52001303658783 37.0 37.0 37.0 37.0 37.0 7 36.40156057258249 37.0 37.0 37.0 37.0 37.0 8 36.35400548813969 37.0 37.0 37.0 37.0 37.0 9 36.41960180790678 37.0 37.0 37.0 37.0 37.0 10-11 36.34957353419952 37.0 37.0 37.0 37.0 37.0 12-13 36.346575049581375 37.0 37.0 37.0 37.0 37.0 14-15 38.21220344018754 40.0 37.0 40.0 37.0 40.0 16-17 38.241069104328105 40.0 37.0 40.0 37.0 40.0 18-19 38.18103415960865 40.0 37.0 40.0 37.0 40.0 20-21 38.19516250960564 40.0 37.0 40.0 37.0 40.0 22-23 38.02885959011802 40.0 37.0 40.0 35.0 40.0 24-25 38.06281528515627 40.0 37.0 40.0 37.0 40.0 26-27 37.94194119513162 40.0 37.0 40.0 33.0 40.0 28-29 37.76968087432604 40.0 37.0 40.0 33.0 40.0 30-31 37.75483023627601 40.0 37.0 40.0 33.0 40.0 32-33 37.6310512015805 40.0 37.0 40.0 33.0 40.0 34-35 37.60721899315614 40.0 37.0 40.0 33.0 40.0 36-37 37.587752271510894 40.0 37.0 40.0 33.0 40.0 38-39 37.41437780142597 40.0 37.0 40.0 33.0 40.0 40-41 37.32690927353996 40.0 37.0 40.0 33.0 40.0 42-43 37.08577942898634 38.5 37.0 40.0 33.0 40.0 44-45 37.05328549086085 38.5 37.0 40.0 33.0 40.0 46-47 37.096175899528035 40.0 37.0 40.0 33.0 40.0 48-49 36.72192500005206 37.0 37.0 40.0 33.0 40.0 50-51 36.6975556744907 37.0 37.0 40.0 33.0 40.0 52-53 36.66811610199082 37.0 37.0 40.0 33.0 40.0 54-55 36.2606722311655 37.0 37.0 40.0 33.0 40.0 56-57 36.21058184970994 37.0 37.0 40.0 33.0 40.0 58-59 35.9389217117498 37.0 37.0 40.0 33.0 40.0 60-61 35.65211608533065 37.0 37.0 40.0 30.0 40.0 62-63 35.40418888891203 37.0 33.0 38.5 27.0 40.0 64-65 35.17418890973725 37.0 33.0 37.0 27.0 40.0 66-67 34.48610020679443 37.0 33.0 37.0 27.0 40.0 68-69 34.268461557684816 37.0 33.0 37.0 27.0 40.0 70-71 33.73447184812028 37.0 33.0 37.0 27.0 40.0 72-73 32.83830969323756 37.0 33.0 37.0 22.0 38.5 74-75 31.83305097805647 37.0 33.0 37.0 15.0 37.0 76-77 29.437094819309976 33.0 27.0 35.0 15.0 37.0 78-79 31.310609547252774 33.0 33.0 37.0 15.0 37.0 80-81 31.52211534256446 37.0 33.0 37.0 15.0 37.0 82-83 31.43600271005532 37.0 33.0 37.0 15.0 37.0 84-85 31.26652290323006 37.0 33.0 37.0 15.0 37.0 86-87 31.118792609268198 33.0 33.0 37.0 15.0 37.0 88-89 30.903505301059795 33.0 33.0 37.0 15.0 37.0 90-91 30.65098388752715 33.0 33.0 37.0 15.0 37.0 92-93 30.625358107015252 33.0 33.0 37.0 15.0 37.0 94-95 30.558349316689817 33.0 33.0 37.0 15.0 37.0 96-97 30.39613438792248 33.0 30.0 37.0 15.0 37.0 98-99 30.242125463621463 33.0 27.0 37.0 15.0 37.0 100-101 28.807051731929437 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.12663389894471777 2306 2 0.15494566338946214 2306 3 0.10427956117550963 2306 4 0.14786021709281982 2306 5 0.1720775016699676 2306 6 0.1913797432684916 2306 7 0.20044432109730792 2306 8 0.197753740972324 2306 9 0.28692186277616116 2306 10-11 0.23923548086155932 2306 12-13 0.2044419031921194 2306 14-15 0.3458939842166231 2306 16-17 0.4249271892362998 2306 18-19 0.42784438663273505 2306 20-21 0.349635536394878 2306 22-23 0.38632381273496463 2306 24-25 0.38374220687414606 2306 26-27 0.43670287109119954 2306 28-29 0.48241540744041345 2306 30-31 0.47690392276437166 2306 32-33 0.48976742975983 2306 34-35 0.4242687384468198 2306 36-37 0.4766958125534302 2306 38-39 0.48461433295422296 2306 40-41 0.5268978383215597 2306 42-43 0.5766013886945984 2306 44-45 0.5624886020106104 2306 46-47 0.5315396518586653 2306 48-49 0.5466173444617795 2306 50-51 0.529511591281036 2306 52-53 0.3771838618521244 2306 54-55 0.3853750735139414 2306 56-57 0.48086455388477134 2306 58-59 0.4693287771241401 2306 60-61 0.5418575246276234 2306 62-63 0.3429666322469629 2306 64-65 0.4868903659864898 2306 66-67 0.50497362078967 2306 68-69 0.5613390021381903 2306 70-71 0.47991255899396634 2306 72-73 0.12147390948920389 2306 74-75 0.38668612538382163 2306 76-77 0.07986022286279848 2306 78-79 0.6396794965894905 2306 80-81 0.7163604627570344 2306 82-83 0.7365879136804985 2306 84-85 0.7629124521622153 2306 86-87 0.6632736460189186 2306 88-89 0.765742695332964 2306 90-91 0.859466928199307 2306 92-93 0.7369828110697014 2306 94-95 0.6235125845563942 2306 96-97 0.7903299531945542 2306 98-99 0.6605035365481626 2306 100-101 0.6727023864968906 2307 1 0.15046700996006734 2307 2 0.15276934637741846 2307 3 0.148142416374057 2307 4 0.11878233351016831 2307 5 0.20373776364686336 2307 6 0.21470359498130875 2307 7 0.1403238674377576 2307 8 0.17514890955229845 2307 9 0.20194211443326537 2307 10-11 0.2330737050347338 2307 12-13 0.1850293835270307 2307 14-15 0.34192422943319656 2307 16-17 0.3949602518308808 2307 18-19 0.36551901218974336 2307 20-21 0.3096795263779555 2307 22-23 0.41146026487646736 2307 24-25 0.47596628096725624 2307 26-27 0.47214013956190115 2307 28-29 0.4515360471179619 2307 30-31 0.4539060231785683 2307 32-33 0.49163480734551257 2307 34-35 0.5248445529298635 2307 36-37 0.47831117782639865 2307 38-39 0.3807947947088266 2307 40-41 0.39772788050744623 2307 42-43 0.43649731209631426 2307 44-45 0.4164990657562626 2307 46-47 0.5249234873626705 2307 48-49 0.5462138069417648 2307 50-51 0.41381101855127156 2307 52-53 0.4706652866938512 2307 54-55 0.5720260860033193 2307 56-57 0.4982634989103758 2307 58-59 0.4254555460477363 2307 60-61 0.41081588931449176 2307 62-63 0.316746041083654 2307 64-65 0.411784527358499 2307 66-67 0.2834810647809576 2307 68-69 0.22859263813556652 2307 70-71 0.35863284869595446 2307 72-73 0.07058621107390906 2307 74-75 -0.13914850181836158 2307 76-77 -0.0484352634433769 2307 78-79 0.4033565394456069 2307 80-81 0.32792255584267593 2307 82-83 0.2673907168500982 2307 84-85 0.21443625732379346 2307 86-87 0.3146470476897143 2307 88-89 0.44207111149727396 2307 90-91 0.5591228416551388 2307 92-93 0.30240290792992397 2307 94-95 0.3512803032373668 2307 96-97 0.23059602435438364 2307 98-99 0.10639071616956386 2307 100-101 0.11655168762075974 2201 1 0.14381051013494073 2201 2 0.17513379391491668 2201 3 0.0870144295684625 2201 4 0.09179558295226542 2201 5 0.15627323071499433 2201 6 0.10210535151326638 2201 7 0.1902062504554607 2201 8 0.17486911428574103 2201 9 0.10358129571255148 2201 10-11 0.1363695547974615 2201 12-13 0.17613407773175283 2201 14-15 0.3617896045914222 2201 16-17 0.25613901366581615 2201 18-19 0.26648462542249973 2201 20-21 0.3460524671930898 2201 22-23 0.3718491731955851 2201 24-25 0.21915683468138525 2201 26-27 0.25929308069659385 2201 28-29 0.33812247385189664 2201 30-31 0.3650242455554391 2201 32-33 0.3786449215183083 2201 34-35 0.39717793183053374 2201 36-37 0.3778319219113726 2201 38-39 0.32013372513106475 2201 40-41 0.3704635538713319 2201 42-43 0.3751492705744184 2201 44-45 0.43849166609464163 2201 46-47 0.3604335189816652 2201 48-49 0.514586733554097 2201 50-51 0.4506095109234707 2201 52-53 0.28841595289419075 2201 54-55 0.387556143577946 2201 56-57 0.4275682360785851 2201 58-59 0.4454599311639029 2201 60-61 0.4630696082303203 2201 62-63 0.49942829414569445 2201 64-65 0.43689357827377506 2201 66-67 0.38262827508367536 2201 68-69 0.4176233676804202 2201 70-71 0.3257310735228174 2201 72-73 0.07185620818008687 2201 74-75 0.330128788428393 2201 76-77 0.20378232708618782 2201 78-79 0.5520900042732464 2201 80-81 0.6040301527457537 2201 82-83 0.42310983151822157 2201 84-85 0.434108474328454 2201 86-87 0.5247396499809831 2201 88-89 0.6479066891697549 2201 90-91 0.7188996090611219 2201 92-93 0.46586841020326375 2201 94-95 0.22149702029440732 2201 96-97 0.44205440392080675 2201 98-99 0.38752956812129824 2201 100-101 0.38366278374001794 1101 1 0.07689611378840766 1101 2 0.1483808140088101 1101 3 0.1314328547338519 1101 4 0.07177065447711328 1101 5 0.12240664301892679 1101 6 0.07910803874474226 1101 7 0.09825181946464312 1101 8 0.12335567457873964 1101 9 0.1876748393591825 1101 10-11 0.18678557027785558 1101 12-13 0.17367359937141913 1101 14-15 0.21260726804213448 1101 16-17 0.20161853861169732 1101 18-19 0.25439073154667113 1101 20-21 0.3039733262146811 1101 22-23 0.23106971717994185 1101 24-25 0.23067247853345663 1101 26-27 0.1855119441893578 1101 28-29 0.17788966271839257 1101 30-31 0.29186361645457026 1101 32-33 0.23663340925504883 1101 34-35 0.20546641317795178 1101 36-37 0.37386403819828473 1101 38-39 0.2572040309359167 1101 40-41 0.3584647391063953 1101 42-43 0.3274323186366104 1101 44-45 0.2914340307434031 1101 46-47 0.35823275449490666 1101 48-49 0.4216349438187166 1101 50-51 0.22361020428260758 1101 52-53 0.34119457863678093 1101 54-55 0.40831956207173903 1101 56-57 0.28134729690319205 1101 58-59 0.4105611646400007 1101 60-61 0.48065003163576847 1101 62-63 0.3136598537845501 1101 64-65 0.30093560510011486 1101 66-67 0.2785867959130144 1101 68-69 0.48551162766677436 1101 70-71 0.38047863975111795 1101 72-73 0.17120137087608356 1101 74-75 0.2922258600593928 1101 76-77 0.04858338439971277 1101 78-79 0.3525977182739304 1101 80-81 0.4752456238158409 1101 82-83 0.4316455692628196 1101 84-85 0.3125587473618552 1101 86-87 0.39965112076439624 1101 88-89 0.4318333668639056 1101 90-91 0.4827930758331256 1101 92-93 0.3595825569269806 1101 94-95 0.3190755708526609 1101 96-97 0.35835647389024317 1101 98-99 0.2550033169900665 1101 100-101 0.38609461159493463 2304 1 0.07863042160610689 2304 2 0.11688484019850875 2304 3 0.09106713522734822 2304 4 0.08407503753308987 2304 5 0.12367689568358031 2304 6 0.1182511695565438 2304 7 0.16115032682731112 2304 8 0.051114242570605484 2304 9 0.18323595518688052 2304 10-11 0.12865086434803885 2304 12-13 0.10698266026064829 2304 14-15 0.3122786068790049 2304 16-17 0.24060098049508838 2304 18-19 0.25900296784733 2304 20-21 0.2598504903437586 2304 22-23 0.29847931255059024 2304 24-25 0.2708840373450556 2304 26-27 0.19107447105933062 2304 28-29 0.2558823897943441 2304 30-31 0.17040315780762683 2304 32-33 0.2308301242794144 2304 34-35 0.23977802196592535 2304 36-37 0.34057130575816785 2304 38-39 0.24429417388534347 2304 40-41 0.2648684327966464 2304 42-43 0.2940121676629488 2304 44-45 0.38799446967980344 2304 46-47 0.28330452180412635 2304 48-49 0.4115080805782867 2304 50-51 0.22553933661116332 2304 52-53 0.37331049867016475 2304 54-55 0.5195586947259159 2304 56-57 0.3806620998063863 2304 58-59 0.3427861454996872 2304 60-61 0.3242227025940778 2304 62-63 0.4166288157309239 2304 64-65 0.44082684001065786 2304 66-67 0.3006801471352816 2304 68-69 0.2756995511269267 2304 70-71 0.4293854123293315 2304 72-73 0.13398422625619588 2304 74-75 0.32925525897582375 2304 76-77 0.3690052089607505 2304 78-79 0.5292620206189156 2304 80-81 0.6116162531831684 2304 82-83 0.5200522445467577 2304 84-85 0.7240648454398375 2304 86-87 0.6322487772386829 2304 88-89 0.6054065899691956 2304 90-91 0.7092582827867808 2304 92-93 0.4341994793981492 2304 94-95 0.43084616463718106 2304 96-97 0.6344503344720316 2304 98-99 0.5147850097153963 2304 100-101 0.4047223360116128 2202 1 0.11480147749768577 2202 2 0.1586658600141675 2202 3 0.11075413414734925 2202 4 0.043244328226208495 2202 5 0.09892838683884264 2202 6 0.08752436508039096 2202 7 0.10285304858541622 2202 8 0.18420447207581248 2202 9 0.17933031574171565 2202 10-11 0.1835602260755067 2202 12-13 0.11932618361441882 2202 14-15 0.1527523064511982 2202 16-17 0.1711472215634089 2202 18-19 0.055666000209789956 2202 20-21 0.1660273616121941 2202 22-23 0.18802876420686943 2202 24-25 0.232004603855394 2202 26-27 0.20203458169117994 2202 28-29 0.22951050122313887 2202 30-31 0.17843234791163098 2202 32-33 0.2954432252721446 2202 34-35 0.3370652332096782 2202 36-37 0.26618800405038456 2202 38-39 0.2876639458358241 2202 40-41 0.32752724368073416 2202 42-43 0.3418848498750293 2202 44-45 0.3380916989578324 2202 46-47 0.3038280968713778 2202 48-49 0.44466058093301797 2202 50-51 0.2682775042307597 2202 52-53 0.35742422715014754 2202 54-55 0.4005352075142241 2202 56-57 0.4975117682710817 2202 58-59 0.30572888793142994 2202 60-61 0.3453170080094381 2202 62-63 0.24672908870810062 2202 64-65 0.3965108011130596 2202 66-67 0.24650222895197516 2202 68-69 0.311275321223242 2202 70-71 0.30514426019363583 2202 72-73 0.1519980728817032 2202 74-75 0.209562672588806 2202 76-77 0.14094340569399222 2202 78-79 0.49500577497034115 2202 80-81 0.5516785387365495 2202 82-83 0.4396555785001013 2202 84-85 0.4903760217707358 2202 86-87 0.5307048718786085 2202 88-89 0.48026776714556974 2202 90-91 0.6756633432674342 2202 92-93 0.5793581965451295 2202 94-95 0.4277512960639669 2202 96-97 0.35233228746819734 2202 98-99 0.25080432176586953 2202 100-101 0.30108011622860076 1102 1 0.08091335493524099 1102 2 0.12569989657843905 1102 3 0.1023552740676692 1102 4 0.021358278246161433 1102 5 0.10335853170955289 1102 6 0.06994242455510857 1102 7 0.04693603590485651 1102 8 0.0722661173981507 1102 9 0.021642505467028172 1102 10-11 0.09903842544952823 1102 12-13 0.09663175648039868 1102 14-15 0.195342824386735 1102 16-17 0.15001143240665726 1102 18-19 0.18918756977084428 1102 20-21 0.16818116683145945 1102 22-23 0.10908613132702527 1102 24-25 0.2065188423564166 1102 26-27 0.26706535533857334 1102 28-29 0.2750999618615779 1102 30-31 0.24637173888049801 1102 32-33 0.11956500349182164 1102 34-35 0.17387247604491307 1102 36-37 0.1507189072341859 1102 38-39 0.08326530981910452 1102 40-41 0.22208566459701018 1102 42-43 0.2274523943550335 1102 44-45 0.18232137749750166 1102 46-47 0.2629257533128424 1102 48-49 0.3696947155248438 1102 50-51 0.21844520135365286 1102 52-53 0.2324639633160146 1102 54-55 0.2855702523618362 1102 56-57 0.33776853042125055 1102 58-59 0.1966858468368784 1102 60-61 0.09275673857373334 1102 62-63 0.17112884503962533 1102 64-65 0.11313662296900162 1102 66-67 0.21672916272036247 1102 68-69 0.2149454025160864 1102 70-71 0.08987857053483594 1102 72-73 0.02391983289626154 1102 74-75 -0.051746555304227115 1102 76-77 -0.15573941217783016 1102 78-79 0.17221191989706242 1102 80-81 0.18872279817345472 1102 82-83 0.09465568352961284 1102 84-85 0.15066156200754222 1102 86-87 0.08192216393767637 1102 88-89 0.17383926073526723 1102 90-91 0.18294678991594182 1102 92-93 0.10890649961829268 1102 94-95 0.11658490830900803 1102 96-97 0.026232103316448985 1102 98-99 -0.02375192854046304 1102 100-101 -0.02010713738966885 2305 1 0.09543995925301374 2305 2 0.11956468005386256 2305 3 0.06920061062448468 2305 4 0.09643700963692936 2305 5 0.16743819621309086 2305 6 0.0449026612264376 2305 7 0.05237062257830871 2305 8 0.06992962420563487 2305 9 0.051802725172670705 2305 10-11 0.03717106000863879 2305 12-13 0.01048622799924459 2305 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21.0 12 46.0 13 100.0 14 240.0 15 586.0 16 1130.0 17 1866.0 18 2896.0 19 4087.0 20 5776.0 21 7670.0 22 10745.0 23 14296.0 24 19009.0 25 25358.0 26 35846.0 27 53888.0 28 94367.0 29 137813.0 30 92971.0 31 60887.0 32 76860.0 33 108038.0 34 166281.0 35 287894.0 36 567339.0 37 866954.0 38 237933.0 39 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99789916623812 22.91643848524392 21.456738019214875 13.628924329303086 2 27.712155195094134 30.013758528795382 29.72081709833877 12.553269177771714 3 34.182446977254 17.303640734408333 33.30216492047195 15.211747367865714 4 26.978519030459637 17.45875926686381 30.193669947088146 25.369051755588405 5 38.23683967565414 16.253181920099184 30.14783823801977 15.362140166226906 6 33.049034369249206 17.111215699994656 34.354810382899444 15.484939547856701 7 29.485006188561265 27.441010828420314 26.230857284071966 16.84312569894645 8 26.47763614314146 34.4987126543062 22.8301335382535 16.193517664298838 9 36.31432476653193 23.016449841415948 23.396614235704007 17.272611156348116 10-11 26.432405218297678 30.79530428875955 26.945325053725988 15.826965439216787 12-13 21.259946645785913 29.198919726412143 26.8553188653587 22.685814762443243 14-15 20.542986378223485 29.7947292756086 27.50688794157276 22.155396404595155 16-17 24.971209013192833 25.173995439257784 28.23050838045059 21.624287167098792 18-19 19.2117375126318 25.309918377270797 33.51068325303185 21.967660857065553 20-21 24.30589541157924 25.815342078537455 33.109601051257116 16.76916145862619 22-23 18.89581558851031 36.22694561354917 27.904719064308974 16.97251973363155 24-25 19.29583335936486 25.35781199130061 33.04485197086413 22.3015026784704 26-27 19.360808046309735 25.622413767969558 27.72848563858108 27.28829254713962 28-29 24.23882084826675 31.026037772784353 27.5902929483722 17.144848430576697 30-31 18.641869382830716 26.462017228010478 32.45765017933985 22.438463209818952 32-33 18.251535339357375 31.75375773743701 27.66764128697084 22.32706563623477 34-35 23.632511917232247 26.444975256167563 28.058305063096945 21.864207763503245 36-37 18.37414382313557 26.487528122724413 33.39462889804736 21.74369915609266 38-39 18.930055507425056 31.465771578199103 27.565500288783003 22.038672625592838 40-41 18.94685378451642 26.06611859123939 37.56811820629418 17.418909417950005 42-43 18.607220489146705 31.220454436986678 32.565126278293846 17.607198795572778 44-45 18.32779403421737 26.1675784484328 32.8784461246397 22.62618139271013 46-47 23.64846524188538 26.227927981089312 27.55221440447636 22.571392372548953 48-49 18.87070507950839 31.66900364283645 32.0201488479393 17.440142429715856 50-51 24.117145389030835 26.426734495743965 31.740106707227124 17.71601340799808 52-53 18.54290509892619 26.259580087614665 26.88467760452815 28.312837208930997 54-55 23.856032224586173 26.419671911470566 31.892408125092103 17.831887738851155 56-57 18.789969453018436 26.306891106034026 31.890298877166696 23.01284056378084 58-59 18.668720616170965 26.20005975062706 32.4916167717684 22.639602861433573 60-61 23.476262780301287 26.468822218607706 32.399677054313166 17.655237946777845 62-63 18.323914620642253 26.591716161191897 31.97487494749704 23.109494270668808 64-65 23.454113889720592 26.45461291120455 31.939752919275683 18.151520279799175 66-67 18.5783655293253 36.430277274477326 26.68060786736429 18.310749328833083 68-69 18.481808539631675 36.828216162356114 26.51116970769025 18.178805590321957 70-71 18.129327527824813 37.13797017137036 26.68475651270913 18.0479457880957 72-73 18.057649402604824 36.681301464156725 26.692943687708592 18.56810544552986 74-75 18.626079191268836 35.7479742487065 26.51744725824831 19.108499301776355 76-77 18.899566633035185 34.8071930166882 26.86441189929443 19.428828450982174 78-79 19.230198418091806 34.03117886998077 27.10856433855342 19.630058373374005 80-81 18.870051790450006 34.04585121006373 27.033625609197315 20.050471390288944 82-83 18.592198165540115 33.4894445319892 27.256421755003185 20.6619355474675 84-85 18.827954520249236 32.69563032073634 27.73092587993314 20.745489279081283 86-87 18.85065009574502 32.601235319921685 27.986500742195318 20.56161384213798 88-89 19.27605498502023 32.943969806808774 27.496467919473943 20.28350728869705 90-91 19.692280880574238 32.261748372842895 27.157050690092362 20.888920056490505 92-93 19.326987299540793 32.46408284579457 27.406643351847563 20.802286502817065 94-95 19.002583521715124 32.42580188138719 27.45606450470347 21.115550092194223 96-97 19.18813794276776 33.112861238686406 27.02876541934633 20.67023539919951 98-99 19.54280618899888 32.38078256782792 26.985140020552677 21.091271222620517 100-101 19.6598662014 32.29538402989938 26.531537013717887 21.513212754982725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 161.0 1 107.0 2 80.5 3 174.0 4 193.5 5 133.0 6 110.5 7 105.5 8 112.0 9 104.5 10 105.0 11 108.5 12 106.0 13 118.0 14 124.5 15 141.0 16 160.0 17 193.0 18 262.0 19 345.0 20 505.0 21 728.0 22 1132.0 23 1858.5 24 2863.0 25 4337.0 26 7065.5 27 11317.0 28 15924.0 29 20288.0 30 25010.0 31 29570.5 32 36037.5 33 47578.0 34 64311.0 35 88622.5 36 116986.0 37 149785.0 38 187603.5 39 208602.5 40 211062.0 41 201803.0 42 188127.0 43 183023.5 44 171027.5 45 155195.0 46 149124.5 47 133202.0 48 103492.0 49 78798.5 50 63300.5 51 50962.0 52 40072.5 53 32098.0 54 25952.5 55 20120.0 56 14482.0 57 10106.5 58 7140.0 59 5343.5 60 4008.5 61 2869.5 62 2022.0 63 1434.5 64 967.0 65 672.0 66 497.5 67 353.5 68 253.0 69 173.0 70 129.5 71 97.5 72 69.0 73 47.5 74 28.0 75 17.5 76 15.5 77 11.0 78 6.0 79 6.5 80 3.5 81 1.5 82 2.0 83 4.0 84 3.5 85 1.5 86 1.5 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17774325419055492 2 0.0 3 0.0 4 0.010169649185282677 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0023428372696470334 12-13 0.0 14-15 0.0 16-17 4.1650440349280595E-4 18-19 1.735435014553358E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0022907742192104326 40-41 0.006681424806030428 42-43 0.002932885174595175 44-45 0.0014404110620792872 46-47 0.007358244461706238 48-49 0.00911103382640513 50-51 0.00537984854511541 52-53 0.007340890111560704 54-55 0.020426070121293025 56-57 0.02224827688657405 58-59 0.028374362487947405 60-61 0.02643067527164764 62-63 0.011523288496634298 64-65 0.01681636529102204 66-67 0.023983711901127407 68-69 0.027315747129069857 70-71 0.03016186055293736 72-73 0.022855679141667723 74-75 0.03318151747826021 76-77 0.030682491057303373 78-79 0.021293787628569705 80-81 0.00978785348208094 82-83 0.001943687216299761 84-85 0.008173898918546316 86-87 0.028304945087365273 88-89 0.02559766646466203 90-91 0.02214415078570085 92-93 0.04392386021834549 94-95 0.046231988787701454 96-97 0.03521197644528763 98-99 0.04184133820088146 100-101 0.04152895989826186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2881122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.71451291716967 #Duplication Level Percentage of deduplicated Percentage of total 1 90.96402651457635 46.13196297671247 2 4.143600111249672 4.202813227311145 3 1.2922125456532503 1.966017895147814 4 0.7979917479535004 1.6187905123753037 5 0.45211424840188796 1.1464376945307004 6 0.41480106426750885 1.2621860359110182 7 0.25105411068389033 0.8912460857430691 8 0.14840213796906726 0.6020913714374286 9 0.13213639151030607 0.6031109460669845 >10 1.1795668854697892 11.547430530642426 >50 0.11385083775042484 4.0452110495135845 >100 0.09553645758945063 10.43899977790686 >500 0.011792570825356216 3.9440667031214254 >1k 0.0028457058858788616 2.309166028813513 >5k 0.0 0.0 >10k+ 6.867021367444656E-5 9.290469164766424 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 266770 9.259239976647986 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3272 0.11356686735237176 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2950 0.10239066585864812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004338587536383395 0.0 0.0 2 0.0047898006401672685 0.0 0.0 3 0.005414557245406477 0.0 0.0 4 0.005761644248317149 0.0 0.0 5 0.0065946530553027604 0.0 0.0 6 0.007392953161997305 0.0 0.0 7 0.008642466372475724 0.0 0.0 8 0.00989197958295414 0.0 0.0 9 0.01124561889430576 0.0 0.0 10-11 0.017909689350190654 0.0 0.0 12-13 0.023966357550981875 0.0 0.0 14-15 0.029832127900172225 0.0 0.0 16-17 0.03306003702724147 0.0 0.0 18-19 0.038144861619882814 0.0 0.0 20-21 0.043871797167908885 0.0 0.0 22-23 0.05015407192059204 0.0 0.0 24-25 0.05624544882167433 0.0 0.0 26-27 0.06271862142595835 0.0 0.0 28-29 0.07056278769173954 0.0 0.0 30-31 0.13720349225058848 0.0 0.0 32-33 0.14820615024285677 0.0 0.0 34-35 0.15677919921475036 0.0 0.0 36-37 0.16531753948635286 0.0 0.0 38-39 0.1747235972652321 0.0 0.0 40-41 0.18595186180939233 0.0 0.0 42-43 0.19872466351650503 0.0 0.0 44-45 0.21212222182885693 0.0 0.0 46-47 0.2260924736960115 0.0 0.0 48-49 0.2406180647678231 0.0 0.0 50-51 0.2574691387591362 0.0 0.0 52-53 0.2749102606553975 0.0 0.0 54-55 0.2930629109076256 0.0 0.0 56-57 0.31352368972920963 0.0 0.0 58-59 0.3365008493218961 0.0 0.0 60-61 0.3607795851754976 0.0 0.0 62-63 0.385023612328808 0.0 0.0 64-65 0.411107200597545 0.0 3.470870029106716E-5 66-67 0.44003690229014947 0.0 3.470870029106716E-5 68-69 0.47071939334745283 0.0 3.470870029106716E-5 70-71 0.5065387720478342 0.0 3.470870029106716E-5 72-73 0.5453430989732473 0.0 3.470870029106716E-5 74-75 0.5890587069898463 0.0 3.470870029106716E-5 76-77 0.6343882695699801 0.0 3.470870029106716E-5 78-79 0.6830151586777651 0.0 3.470870029106716E-5 80-81 0.7376466529359049 0.0 3.470870029106716E-5 82-83 0.7964952542794093 0.0 6.941740058213432E-5 84-85 0.8583635125482365 0.0 6.941740058213432E-5 86-87 0.9312344288093319 0.0 6.941740058213432E-5 88-89 1.0084092239065197 0.0 6.941740058213432E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 34410 0.0 83.67005 1 TCGGAAG 34575 0.0 83.447945 3 CGGAAGA 34705 0.0 82.87534 4 ATCGGAA 34905 0.0 82.30524 2 GAAGAGC 35320 0.0 81.51794 6 GGAAGAG 36845 0.0 78.2084 5 GAGCACA 42505 0.0 67.80527 9 AGAGCAC 42470 0.0 67.80524 8 AAGAGCA 43510 0.0 66.15178 7 TCGGACG 120 0.0 59.36652 3 GCCGTCT 31460 0.0 45.48811 50-51 ATGCCGT 31505 0.0 45.36879 48-49 CGTCTTC 31580 0.0 45.368305 52-53 TCGTATG 31825 0.0 45.275543 44-45 GTATGCC 31750 0.0 45.150997 46-47 TATGCCG 31475 0.0 45.125282 48-49 TCTCGTA 31760 0.0 45.112785 42-43 TGCCGTC 31530 0.0 45.070732 50-51 CGTATGC 31670 0.0 45.032574 46-47 CTCGGAA 285 0.0 44.993572 2 >>END_MODULE