FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881122
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2667709.259239976647986TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32720.11356686735237176No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29500.10239066585864812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA344100.083.670051
TCGGAAG345750.083.4479453
CGGAAGA347050.082.875344
ATCGGAA349050.082.305242
GAAGAGC353200.081.517946
GGAAGAG368450.078.20845
GAGCACA425050.067.805279
AGAGCAC424700.067.805248
AAGAGCA435100.066.151787
TCGGACG1200.059.366523
GCCGTCT314600.045.4881150-51
ATGCCGT315050.045.3687948-49
CGTCTTC315800.045.36830552-53
TCGTATG318250.045.27554344-45
GTATGCC317500.045.15099746-47
TATGCCG314750.045.12528248-49
TCTCGTA317600.045.11278542-43
TGCCGTC315300.045.07073250-51
CGTATGC316700.045.03257446-47
CTCGGAA2850.044.9935722
Produced by FastQC (version 0.11.2)