##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_30749-1.34100000035870.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4906452 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62095583529606 33.0 33.0 33.0 33.0 33.0 2 32.59648234610264 33.0 33.0 33.0 33.0 33.0 3 32.68954144461212 33.0 33.0 33.0 33.0 33.0 4 36.59497596226357 37.0 37.0 37.0 37.0 37.0 5 36.57588090131117 37.0 37.0 37.0 37.0 37.0 6 36.47397936431458 37.0 37.0 37.0 37.0 37.0 7 36.21738478232336 37.0 37.0 37.0 33.0 37.0 8 36.19132134585236 37.0 37.0 37.0 33.0 37.0 9 36.328580204188285 37.0 37.0 37.0 37.0 37.0 10-11 36.201731719784476 37.0 37.0 37.0 35.0 37.0 12-13 36.27576974155663 37.0 37.0 37.0 35.0 37.0 14-15 38.1116716315578 40.0 37.0 40.0 35.0 40.0 16-17 38.20966270535206 40.0 37.0 40.0 37.0 40.0 18-19 38.05376023244495 40.0 37.0 40.0 37.0 40.0 20-21 38.17479963117951 40.0 37.0 40.0 37.0 40.0 22-23 37.61724612815941 40.0 37.0 40.0 33.0 40.0 24-25 38.01020299393534 40.0 37.0 40.0 37.0 40.0 26-27 38.07463295269168 40.0 37.0 40.0 37.0 40.0 28-29 37.6879236768239 40.0 37.0 40.0 33.0 40.0 30-31 37.62176517777 40.0 37.0 40.0 33.0 40.0 32-33 37.325476637700724 40.0 37.0 40.0 33.0 40.0 34-35 37.32288892258602 40.0 37.0 40.0 33.0 40.0 36-37 37.53886943151589 40.0 37.0 40.0 33.0 40.0 38-39 37.18970245709119 40.0 37.0 40.0 33.0 40.0 40-41 36.56249271367579 37.0 37.0 40.0 33.0 40.0 42-43 36.340712392580215 37.0 37.0 40.0 33.0 40.0 44-45 36.33277733074735 37.0 37.0 40.0 33.0 40.0 46-47 36.87100495429284 37.0 37.0 40.0 33.0 40.0 48-49 36.38422051209305 37.0 37.0 40.0 33.0 40.0 50-51 36.566986898068095 37.0 37.0 40.0 33.0 40.0 52-53 36.593624884132154 37.0 37.0 40.0 33.0 40.0 54-55 35.93345904535497 37.0 35.0 40.0 30.0 40.0 56-57 35.97243680362102 37.0 37.0 40.0 33.0 40.0 58-59 35.9932579591118 37.0 37.0 40.0 33.0 40.0 60-61 35.66481349455778 37.0 35.0 40.0 27.0 40.0 62-63 35.477472214137634 37.0 33.0 40.0 27.0 40.0 64-65 35.0644591040532 37.0 33.0 38.5 27.0 40.0 66-67 33.54117048327386 37.0 33.0 37.0 27.0 40.0 68-69 33.49750023030899 37.0 33.0 37.0 27.0 40.0 70-71 32.62077953682213 35.0 33.0 37.0 22.0 37.0 72-73 30.350492881617917 33.0 27.0 37.0 15.0 37.0 74-75 28.474878588438244 33.0 22.0 37.0 10.5 37.0 76-77 26.22288468327011 30.0 22.0 33.0 6.0 37.0 78-79 27.446596440768197 33.0 18.5 37.0 6.0 37.0 80-81 27.41326318896017 33.0 15.0 37.0 6.0 37.0 82-83 27.229487825418452 33.0 15.0 37.0 6.0 37.0 84-85 27.014838726639944 33.0 15.0 37.0 6.0 37.0 86-87 26.859145977582173 33.0 15.0 37.0 6.0 37.0 88-89 26.65745695667664 33.0 15.0 37.0 6.0 37.0 90-91 26.393484130691586 33.0 15.0 37.0 6.0 37.0 92-93 26.354718847753936 33.0 15.0 37.0 6.0 37.0 94-95 26.273833923168922 33.0 15.0 37.0 6.0 37.0 96-97 26.120911505910993 33.0 15.0 37.0 6.0 37.0 98-99 25.974152605589538 33.0 15.0 37.0 6.0 37.0 100-101 24.742893846714487 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1336968785103636 2306 2 0.17434234180933572 2306 3 0.11009848699330149 2306 4 0.1438177395039446 2306 5 0.17036307437263076 2306 6 0.09459457694304518 2306 7 0.17664906468021968 2306 8 0.11315460416038547 2306 9 0.18127005721588318 2306 10-11 0.13186892763474134 2306 12-13 0.18018885521584593 2306 14-15 0.3294828911993477 2306 16-17 0.3427490441179728 2306 18-19 0.3984900217481382 2306 20-21 0.37827392776583935 2306 22-23 0.36240336858917743 2306 24-25 0.33742318644741687 2306 26-27 0.3383185151748265 2306 28-29 0.4333309206079363 2306 30-31 0.3821695512482748 2306 32-33 0.44411059144476184 2306 34-35 0.3370120084849475 2306 36-37 0.4809247764429543 2306 38-39 0.45042605243052236 2306 40-41 0.3613701964239837 2306 42-43 0.33430676869930664 2306 44-45 0.4382627724507202 2306 46-47 0.42435888990267756 2306 48-49 0.5738832197355919 2306 50-51 0.38613261230674567 2306 52-53 0.31992429374184894 2306 54-55 0.4023613316655883 2306 56-57 0.4333469426317791 2306 58-59 0.36422701571589755 2306 60-61 0.30454063055764635 2306 62-63 0.3328440624033959 2306 64-65 0.32523965066089744 2306 66-67 0.1454692181628161 2306 68-69 0.4626646173323863 2306 70-71 0.09545420141001415 2306 72-73 -0.7711997615090844 2306 74-75 -0.6317050674977658 2306 76-77 -0.46931607712287615 2306 78-79 0.037717567619313996 2306 80-81 0.049136516845237566 2306 82-83 0.013811819671985859 2306 84-85 -0.022043941290117886 2306 86-87 -0.06502432837984173 2306 88-89 0.07021884430657721 2306 90-91 0.11043194315350746 2306 92-93 -0.07583731773827651 2306 94-95 -0.14418633645106738 2306 96-97 -0.0753912014895306 2306 98-99 -0.20341599352725126 2306 100-101 -0.32849627840954554 2307 1 0.16557862172133753 2307 2 0.17229205865687902 2307 3 0.11523888215261735 2307 4 0.1591358857350471 2307 5 0.2016833526181685 2307 6 0.16914735312779783 2307 7 0.12279880612324945 2307 8 0.19143246980816286 2307 9 0.20944177874143577 2307 10-11 0.21306111455066912 2307 12-13 0.20515448850692763 2307 14-15 0.30423819962306453 2307 16-17 0.3058387725814029 2307 18-19 0.34786670279464005 2307 20-21 0.3250888583201217 2307 22-23 0.3476706587878766 2307 24-25 0.35159153948757904 2307 26-27 0.38963814809331865 2307 28-29 0.383905413874011 2307 30-31 0.3540763648059908 2307 32-33 0.2668800428802527 2307 34-35 0.348187352973909 2307 36-37 0.31100894900583853 2307 38-39 0.3512163801957726 2307 40-41 0.34206198092370954 2307 42-43 0.2977631464177506 2307 44-45 0.40867445153079984 2307 46-47 0.37518329519728866 2307 48-49 0.5178953914558946 2307 50-51 0.24404887260001829 2307 52-53 0.07765931454451191 2307 54-55 0.3196709662765471 2307 56-57 0.28020566106098244 2307 58-59 0.38365858913280704 2307 60-61 0.29202569975984005 2307 62-63 0.18268901649386038 2307 64-65 0.15580180661330445 2307 66-67 0.03220846281359968 2307 68-69 0.022716027371970426 2307 70-71 0.06415005634050175 2307 72-73 -0.40474813790277864 2307 74-75 -0.06448390661529757 2307 76-77 0.12754697899987733 2307 78-79 0.6242480094004641 2307 80-81 0.6671181286773731 2307 82-83 0.5840701270400359 2307 84-85 0.5960837062249453 2307 86-87 0.49601097709728137 2307 88-89 0.6153531545189388 2307 90-91 0.8041696225818242 2307 92-93 0.7066264374075999 2307 94-95 0.6621983527387307 2307 96-97 0.52082394019628 2307 98-99 0.39678891302817476 2307 100-101 0.34159625296491924 2201 1 0.16967421428996943 2201 2 0.157621504030665 2201 3 0.1099159265042644 2201 4 0.18637855739842024 2201 5 0.17420734168699425 2201 6 0.12008257573801018 2201 7 0.09321642538994723 2201 8 0.15801126518115893 2201 9 0.18907471998841174 2201 10-11 0.2294196881859918 2201 12-13 0.18487549200826692 2201 14-15 0.28094228136363597 2201 16-17 0.3458006744558091 2201 18-19 0.37940789947767684 2201 20-21 0.44006426699067447 2201 22-23 0.4112143323778952 2201 24-25 0.3532815048267466 2201 26-27 0.3537979122656836 2201 28-29 0.38062434207338214 2201 30-31 0.4171804248651867 2201 32-33 0.4503841538231228 2201 34-35 0.4362364237004641 2201 36-37 0.4316194238751194 2201 38-39 0.25707985006306444 2201 40-41 0.43768899592617316 2201 42-43 0.4684384450949466 2201 44-45 0.4201469655311527 2201 46-47 0.5389208452950882 2201 48-49 0.575997346540774 2201 50-51 0.4736230199899367 2201 52-53 0.4122646296560859 2201 54-55 0.5421343987742944 2201 56-57 0.5775982838677933 2201 58-59 0.5793557234298703 2201 60-61 0.4879167693218278 2201 62-63 0.5175934788631409 2201 64-65 0.4911432751656051 2201 66-67 0.3409528537107107 2201 68-69 0.44409306984339025 2201 70-71 0.4166486447003521 2201 72-73 -0.3206686991391692 2201 74-75 -0.11637938237474899 2201 76-77 0.25724038868223076 2201 78-79 0.3880891336359582 2201 80-81 0.704904106992462 2201 82-83 0.4663391189615176 2201 84-85 0.4491575636484093 2201 86-87 0.2900934433222133 2201 88-89 0.4215306665773717 2201 90-91 0.521823893843532 2201 92-93 0.38264378780165487 2201 94-95 0.35258064721655913 2201 96-97 0.39319261212641266 2201 98-99 0.3801376186966543 2201 100-101 0.2900945234486798 1101 1 0.08950401766505678 1101 2 0.12536816400034922 1101 3 0.10016883068237092 1101 4 0.09572420057314446 1101 5 0.09940035281822901 1101 6 0.15830092580723942 1101 7 0.13607529518632333 1101 8 0.05488770605596471 1101 9 0.1503402036796544 1101 10-11 0.1373727952987167 1101 12-13 0.09547353639620582 1101 14-15 0.1930276213093549 1101 16-17 0.19806395075535477 1101 18-19 0.16916702443352705 1101 20-21 0.2145639337427454 1101 22-23 0.1801846685087014 1101 24-25 0.21571777630115463 1101 26-27 0.1843288135722574 1101 28-29 0.20041196330384992 1101 30-31 0.27235187078104417 1101 32-33 0.18233304009493878 1101 34-35 0.1150727853864737 1101 36-37 0.15612156847396363 1101 38-39 0.2328938192715455 1101 40-41 0.26398236455160173 1101 42-43 0.20845239430095575 1101 44-45 0.24874276181745358 1101 46-47 0.34188419797590086 1101 48-49 0.40100282234022444 1101 50-51 0.27565239377481277 1101 52-53 0.2926409955734428 1101 54-55 0.5001572208143727 1101 56-57 0.24604507205655324 1101 58-59 0.4108376409888308 1101 60-61 0.365586407308065 1101 62-63 0.3697727088119578 1101 64-65 0.3044162328997615 1101 66-67 0.11843986367676962 1101 68-69 0.4336566004176987 1101 70-71 0.38387277966054967 1101 72-73 -0.22817897321804992 1101 74-75 -0.221049988760182 1101 76-77 -0.1283184093084273 1101 78-79 0.0840143439954737 1101 80-81 0.33182850750733195 1101 82-83 0.18355684331395494 1101 84-85 0.17501087774600066 1101 86-87 0.06615093959662843 1101 88-89 0.15363658682028714 1101 90-91 0.14695443005994235 1101 92-93 0.08218060670395744 1101 94-95 -0.09628651372466734 1101 96-97 0.03726440346862958 1101 98-99 0.05189680765489868 1101 100-101 0.11347332912930241 2304 1 0.10997867246734216 2304 2 0.13176405300201566 2304 3 0.07689861878411364 2304 4 0.14121321471313308 2304 5 0.16254264437998955 2304 6 0.14562374129452138 2304 7 0.18452603657041777 2304 8 0.13890940455390677 2304 9 0.10272989977554658 2304 10-11 0.1342572967559903 2304 12-13 0.10311848166328019 2304 14-15 0.22502991471304767 2304 16-17 0.25012710333668764 2304 18-19 0.20245787146859584 2304 20-21 0.23312948527776456 2304 22-23 0.13603316428219614 2304 24-25 0.18399583111084894 2304 26-27 0.14076906680789136 2304 28-29 0.292312203950182 2304 30-31 0.21835491310081068 2304 32-33 0.32460443774501613 2304 34-35 0.35986963055673016 2304 36-37 0.2059625468377817 2304 38-39 0.34215228945931386 2304 40-41 0.24436172844333015 2304 42-43 0.270769491298239 2304 44-45 0.4741560509427103 2304 46-47 0.3006570966217623 2304 48-49 0.3492034009574638 2304 50-51 0.2983050918379675 2304 52-53 0.36289012872449433 2304 54-55 0.41715820371079815 2304 56-57 0.42350322226452164 2304 58-59 0.34096223089208877 2304 60-61 0.32130727162547856 2304 62-63 0.3372428251879356 2304 64-65 0.32163152415800056 2304 66-67 0.2203768132261672 2304 68-69 0.15154014492666334 2304 70-71 0.21999258399136323 2304 72-73 -0.5590460432989701 2304 74-75 -0.2764682764113182 2304 76-77 -0.04762762338562254 2304 78-79 0.13041726199440618 2304 80-81 0.3097120916414795 2304 82-83 0.14644309224207674 2304 84-85 0.23179092976109317 2304 86-87 0.13945747565015054 2304 88-89 0.22445641856278442 2304 90-91 0.29920402554747483 2304 92-93 0.06698086347518029 2304 94-95 -0.010018929807305454 2304 96-97 0.02819416571174216 2304 98-99 -0.050918830464354414 2304 100-101 0.05484105516688231 2202 1 0.05549550399562264 2202 2 0.07953904608438478 2202 3 0.07802025998419992 2202 4 0.03082541867751587 2202 5 0.07703920409024079 2202 6 0.07413975740988121 2202 7 0.04961386621645403 2202 8 0.07903839446813521 2202 9 0.0955204498072888 2202 10-11 0.12590341636968816 2202 12-13 0.05781378099744927 2202 14-15 0.1081686869358407 2202 16-17 0.11950157017925989 2202 18-19 0.016892591533199663 2202 20-21 0.0785325471488747 2202 22-23 0.0038919275892581595 2202 24-25 0.05510821841816238 2202 26-27 -0.011757169463074035 2202 28-29 0.08524040085843154 2202 30-31 0.08320020665681227 2202 32-33 0.04894702761760783 2202 34-35 0.18776569172710822 2202 36-37 0.1774841402834184 2202 38-39 0.16518607249008 2202 40-41 0.03638714886028538 2202 42-43 0.1325928757411745 2202 44-45 0.14572919212911728 2202 46-47 0.18752574339211492 2202 48-49 0.25955088213136435 2202 50-51 0.24951531693368878 2202 52-53 0.2124298012243102 2202 54-55 0.15719588972595488 2202 56-57 0.3242738801024956 2202 58-59 0.2373103689835787 2202 60-61 0.44581267943338787 2202 62-63 0.28845869612862884 2202 64-65 0.2217624077827267 2202 66-67 0.19594118453339604 2202 68-69 0.14538776180668123 2202 70-71 0.040189918035132166 2202 72-73 -0.40758713637572086 2202 74-75 -0.0626134610789677 2202 76-77 -0.10136254096657638 2202 78-79 0.13063830270594323 2202 80-81 0.21597797976965083 2202 82-83 0.07230606111553328 2202 84-85 0.00927285938152167 2202 86-87 0.06916941111343888 2202 88-89 0.0966456331757577 2202 90-91 0.25142468045961053 2202 92-93 0.032215896766551566 2202 94-95 -0.021367401650564943 2202 96-97 -0.041421484570506095 2202 98-99 -0.037490423454215716 2202 100-101 -0.06621091865874718 1102 1 0.12295132022499189 1102 2 0.10112643854827041 1102 3 0.114338065632694 1102 4 0.09163912195808166 1102 5 0.1582138066968639 1102 6 0.11608844678490726 1102 7 0.08728476744808944 1102 8 0.17157029853994743 1102 9 0.15728631252854797 1102 10-11 0.09036405388598467 1102 12-13 0.10546347106568987 1102 14-15 0.15873281754934965 1102 16-17 0.16972798067617845 1102 18-19 0.17780134604401354 1102 20-21 0.1382577477092397 1102 22-23 0.07049330510791663 1102 24-25 0.15087805528617082 1102 26-27 0.18705334120938488 1102 28-29 0.22226355081701143 1102 30-31 0.26551161856942684 1102 32-33 0.12591688413767343 1102 34-35 0.12817203502648056 1102 36-37 0.15031519304040586 1102 38-39 0.145735074991741 1102 40-41 0.17730759805190388 1102 42-43 0.30865958937967974 1102 44-45 0.15296583674969355 1102 46-47 0.27512884217014033 1102 48-49 0.24845368649764055 1102 50-51 0.17975819313222985 1102 52-53 0.054482572690425 1102 54-55 0.20646196188934596 1102 56-57 0.20333821476234704 1102 58-59 0.28061246633001247 1102 60-61 0.186194713245726 1102 62-63 0.17846516824555891 1102 64-65 0.28659404896561824 1102 66-67 0.25142403881932296 1102 68-69 0.09311552054297323 1102 70-71 0.03344502934617566 1102 72-73 -0.11883672068633189 1102 74-75 -0.1318372245113082 1102 76-77 -0.15506918395988833 1102 78-79 0.06384633994489874 1102 80-81 0.20697168401009236 1102 82-83 0.013103770116774882 1102 84-85 0.04558626319532877 1102 86-87 0.03765914731290465 1102 88-89 0.07137452985731585 1102 90-91 0.14998405936357528 1102 92-93 -0.02435940769131406 1102 94-95 -0.029430455234525255 1102 96-97 -0.10994245959676618 1102 98-99 -0.16970310647469233 1102 100-101 0.011198411356453164 2305 1 0.11692479450265125 2305 2 0.1340821762561646 2305 3 0.0759196988939479 2305 4 0.06979574724430648 2305 5 0.05425647145563772 2305 6 -0.0349018277687847 2305 7 -0.011184394489362148 2305 8 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-1.2064825915750426 1213 86-87 -1.246678295146431 1213 88-89 -1.3008059064515614 1213 90-91 -1.1328196484416324 1213 92-93 -1.2050445059741577 1213 94-95 -1.2553331720554688 1213 96-97 -1.1106999024903317 1213 98-99 -1.1961991699865813 1213 100-101 -1.146718538251779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 24.0 9 58.0 10 54.0 11 25.0 12 43.0 13 115.0 14 325.0 15 781.0 16 1593.0 17 2675.0 18 4203.0 19 6690.0 20 10018.0 21 15521.0 22 23250.0 23 34261.0 24 48659.0 25 69553.0 26 103663.0 27 170534.0 28 315848.0 29 505225.0 30 346151.0 31 119910.0 32 118088.0 33 166183.0 34 255635.0 35 438291.0 36 807416.0 37 1059681.0 38 281672.0 39 306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.04558594355314 19.534595897160955 14.63913093098991 12.780687228295989 2 21.76684903877588 45.025325836266205 21.27382475157201 11.934000373385901 3 25.54450751785608 15.637878450660478 45.128292297570624 13.689321733912815 4 21.659069961031978 15.913172120926019 21.257465745522794 41.170292172519204 5 51.466925591038084 15.033327545036618 19.819739396207282 13.680007467718017 6 48.15856753515575 15.800154571979915 22.13617905565977 13.905098837204562 7 22.790460397859796 43.39537001482945 18.677712530357983 15.136457056952763 8 21.154410559809815 46.15785500398251 18.108319412887358 14.579415023320314 9 48.394827871545466 17.266876349753346 19.127100397598916 15.211195381102272 10-11 30.865208440613536 34.72254677331894 21.04777696737666 13.364467818690862 12-13 16.93803383789345 33.78255814996254 20.826077581111566 28.45333043103244 14-15 16.788628524237065 33.86605025382904 20.81001709585664 28.535304126077254 16-17 30.529272913540034 19.781650172523307 21.289852099440356 28.3992248144963 18-19 16.240788659503853 19.92191098577954 35.13700939089998 28.70029096381662 20-21 30.235076181322064 20.25757105134219 34.55432764857376 14.953025118761989 22-23 16.170075647331313 48.17809284590984 20.47605887105387 15.175772635704988 24-25 16.04510754410723 20.146625300726473 34.63393710974855 29.174330045417747 26-27 16.042131870443242 20.258304779094953 20.788504605772154 42.91105874468965 28-29 29.974419397152975 34.22805318384853 20.619808366616038 15.177719052382454 30-31 15.799950758715259 20.881056209252634 34.37301536833541 28.9459776636967 32-33 15.631366616854706 34.522278012706536 20.63011112714442 29.21624424329434 34-35 15.731082256587856 34.35481484380159 34.793757281228885 15.120345618381673 36-37 15.584734141901317 20.826373110345315 34.600817454241884 28.988075293511486 38-39 29.58414011258902 34.52755006787137 20.590796805765603 15.297513013774013 40-41 15.729150307507187 48.12563141472262 20.631033058436607 15.51418521933359 42-43 15.59523133768085 34.125277307947584 34.50092518359215 15.778566170779404 44-45 15.566927364509391 20.520036911105073 34.631034168249215 29.282001556136315 46-47 29.60774496028572 20.423782076479586 20.9653010997621 29.003171863472595 48-49 15.643778781413367 34.485964020972055 34.55996445207221 15.310292745542368 50-51 29.63352872679274 20.6657475517048 34.02004700612637 15.68067671537609 52-53 15.946803833479784 20.520070450453645 20.444766897336667 43.08835881872991 54-55 29.38951737268744 20.466023652825527 34.58608574582437 15.558373228662667 56-57 15.742242768259878 20.47070920626048 34.65190907216354 29.135138953316105 58-59 15.783900540304712 20.592556424661872 34.54424244957164 29.07930058546177 60-61 29.656247626881864 20.45581196698811 34.33291490004745 15.555025506082574 62-63 15.754742950813592 20.574157217828965 34.68530027435118 28.98579955700627 64-65 29.633865072844472 20.791554055121487 34.45611082555843 15.11847004647561 66-67 15.73554114936905 47.98648400709437 20.55442072859968 15.723554114936904 68-69 15.718365385654323 48.233940320360794 20.365566280549228 15.682128013435657 70-71 15.780345064920873 47.881050297570994 20.46873955158033 15.869865085927803 72-73 15.72228775147502 46.99226845420864 20.665769960113003 16.619673834203336 74-75 15.955106645570622 45.310409443188945 20.859845681897486 17.87463822934295 76-77 16.25814874798371 43.803434747223676 21.133707560048343 18.804708944744274 78-79 16.503363256238305 41.91896567066009 21.63486064806222 19.942810425039387 80-81 16.85588136435154 40.30255734890019 22.063700231900707 20.777861054847566 82-83 17.114356322131922 39.163129601793585 22.15428906269904 21.568225013375457 84-85 17.378889388722914 38.36534841613649 22.326155766536697 21.929606428603897 86-87 17.564426754101756 37.76537170463257 22.464628573647826 22.205572967617844 88-89 18.051785608530693 37.266529866389874 22.44508868080831 22.236595844271122 90-91 18.36731032792864 36.45202548116606 22.548118644921228 22.63254554598407 92-93 18.534643068327796 36.11746834410619 22.547132108557797 22.800756479008218 94-95 18.8586346909592 35.67531492756345 22.59539380652144 22.870656574955913 96-97 19.001540128984274 35.554287405928584 22.695912806761726 22.748259658325424 98-99 19.261454845219234 35.13005425885415 22.748836618477128 22.859654277449494 100-101 19.303351606519296 35.7615445062794 22.30638721456048 22.628716672640827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22.0 1 15.5 2 17.0 3 32.0 4 34.0 5 28.0 6 26.0 7 29.5 8 30.5 9 38.5 10 49.5 11 43.5 12 43.0 13 51.0 14 67.0 15 97.0 16 151.0 17 247.5 18 381.5 19 634.0 20 1012.5 21 1554.0 22 2348.0 23 3515.0 24 5237.0 25 7449.5 26 10190.0 27 13533.0 28 17584.5 29 22467.5 30 28041.5 31 34809.0 32 45016.5 33 61248.0 34 86368.0 35 122140.0 36 170590.0 37 228337.5 38 282443.0 39 320490.5 40 340778.0 41 342281.5 42 326246.5 43 300301.0 44 268212.0 45 238587.0 46 215859.5 47 196682.0 48 180535.0 49 162352.5 50 142239.5 51 127532.5 52 115361.0 53 102244.5 54 88224.0 55 73943.0 56 60360.5 57 45817.5 58 32618.5 59 23550.0 60 17182.5 61 12625.0 62 9054.0 63 6174.5 64 4065.5 65 2466.0 66 1529.5 67 990.0 68 678.5 69 462.5 70 313.5 71 219.5 72 152.0 73 116.0 74 80.0 75 49.0 76 38.0 77 29.0 78 21.5 79 21.0 80 18.0 81 8.0 82 8.0 83 5.5 84 3.5 85 4.0 86 3.0 87 3.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1717942007788928 2 0.0 3 0.0 4 0.007704141404012512 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002089085962728261 12-13 0.0 14-15 0.0 16-17 2.751479072861612E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0022827085641518555 40-41 0.00624687656171914 42-43 0.0026190004508349412 44-45 0.001416502189362089 46-47 0.007041748293879162 48-49 0.008142339923023805 50-51 0.005767915389776564 52-53 0.006297829877883244 54-55 0.018485863104336902 56-57 0.02130867681982826 58-59 0.027504600065383293 60-61 0.02546646741881914 62-63 0.010465811140106945 64-65 0.015499998777120412 66-67 0.023479288088419085 68-69 0.02639381777300583 70-71 0.028615382357760764 72-73 0.022297171153411875 74-75 0.03227383045834342 76-77 0.028615382357760764 78-79 0.02063609304646209 80-81 0.00964036741824846 82-83 0.0015693621378544008 84-85 0.007724522730478155 86-87 0.027056210883139184 88-89 0.025425704765887857 90-91 0.020890859627282606 92-93 0.04386061455406066 94-95 0.04542997669191506 96-97 0.03399605254469014 98-99 0.04074227160481749 100-101 0.04072189027835185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4906452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.80220585823873 #Duplication Level Percentage of deduplicated Percentage of total 1 91.48237858462021 48.30471386426446 2 4.47202104724566 4.722651518780833 3 1.3978403837420337 2.214271670979188 4 0.6624337554243874 1.3991185408545865 5 0.44442037119245814 1.173318796364952 6 0.2529286427754465 0.8013114157964429 7 0.18251242049805266 0.6745940879176524 8 0.1490767153740991 0.6297263531082594 9 0.11535893348665509 0.5482085538194307 >10 0.7411721572016097 7.153237671087687 >50 0.06325243559556996 2.323788060049531 >100 0.03450858766262259 3.0142965796766816 >500 8.598740747831E-4 0.3023784133118239 >1k 8.468977162408144E-4 0.9065131546721586 >5k 2.3351630787244528E-4 0.7774583618387882 >10k+ 1.556770822456974E-4 25.054412957477535 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 1181949 24.089688434738584 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 16197 0.3301163447639965 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 10776 0.21962917399375353 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT 10257 0.20905126555808556 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT 7933 0.16168506285193454 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT 7584 0.15457197991542565 TruSeq Adapter, Index 13 (97% over 38bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 6373 0.12989019356553372 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT 5342 0.10887704597945726 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCGAAATCTCGTAT 5326 0.10855094475600699 TruSeq Adapter, Index 27 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT 5274 0.10749111577979362 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005136094269341675 0.0 0.0 2 0.005645627430982714 0.0 0.0 3 0.006440499163142735 0.0 0.0 4 0.007011176304180699 0.0 0.0 5 0.008254437218584834 0.0 0.0 6 0.009191978236004346 0.0 0.0 7 0.010679815067996182 0.0 2.038132646564157E-5 8 0.01247337179697264 0.0 2.038132646564157E-5 9 0.01363510740551421 0.0 2.038132646564157E-5 10-11 0.022531556407766752 0.0 2.038132646564157E-5 12-13 0.02882938628565 0.0 2.038132646564157E-5 14-15 0.034199865809346544 0.0 2.038132646564157E-5 16-17 0.03680867559694867 0.0 2.038132646564157E-5 18-19 0.039886255893260544 0.0 2.038132646564157E-5 20-21 0.04358546664677449 0.0 2.038132646564157E-5 22-23 0.04750887199141049 0.0 2.038132646564157E-5 24-25 0.05164628126393573 1.0190663232820784E-5 2.038132646564157E-5 26-27 0.05585502517909072 2.038132646564157E-5 2.038132646564157E-5 28-29 0.06097073812196675 2.038132646564157E-5 2.038132646564157E-5 30-31 0.111108801227445 2.038132646564157E-5 2.038132646564157E-5 32-33 0.11962819569008318 2.038132646564157E-5 2.038132646564157E-5 34-35 0.12590564424150077 2.038132646564157E-5 2.038132646564157E-5 36-37 0.13319196845296766 2.038132646564157E-5 2.038132646564157E-5 38-39 0.14043753001150322 2.038132646564157E-5 3.057198969846235E-5 40-41 0.14807033677288597 2.038132646564157E-5 4.076265293128314E-5 42-43 0.15662030322522263 2.038132646564157E-5 4.076265293128314E-5 44-45 0.16648486523459316 2.038132646564157E-5 4.076265293128314E-5 46-47 0.17676724443650932 2.038132646564157E-5 4.076265293128314E-5 48-49 0.18844574450132193 2.038132646564157E-5 4.076265293128314E-5 50-51 0.2007356843601038 2.038132646564157E-5 4.076265293128314E-5 52-53 0.21444212640824775 2.038132646564157E-5 4.076265293128314E-5 54-55 0.22890267753562044 2.038132646564157E-5 4.076265293128314E-5 56-57 0.2443721043230424 2.038132646564157E-5 4.076265293128314E-5 58-59 0.2619815703893567 2.038132646564157E-5 6.11439793969247E-5 60-61 0.28145592782727724 2.038132646564157E-5 6.11439793969247E-5 62-63 0.3015519157223998 2.038132646564157E-5 6.11439793969247E-5 64-65 0.32359432029499113 2.038132646564157E-5 6.11439793969247E-5 66-67 0.34667617251733024 2.038132646564157E-5 6.11439793969247E-5 68-69 0.37036946453363856 2.038132646564157E-5 6.11439793969247E-5 70-71 0.3976804419975983 2.038132646564157E-5 6.11439793969247E-5 72-73 0.42727412802570985 2.038132646564157E-5 6.11439793969247E-5 74-75 0.4586104174666337 2.038132646564157E-5 6.11439793969247E-5 76-77 0.49233132210403774 2.038132646564157E-5 6.11439793969247E-5 78-79 0.5301386826978027 2.038132646564157E-5 6.11439793969247E-5 80-81 0.571461822106891 2.038132646564157E-5 6.11439793969247E-5 82-83 0.616789892166478 2.038132646564157E-5 7.13346426297455E-5 84-85 0.6656948850207849 2.038132646564157E-5 8.152530586256628E-5 86-87 0.7183602326080027 2.038132646564157E-5 8.152530586256628E-5 88-89 0.7777208459391838 2.038132646564157E-5 8.152530586256628E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 141060 0.0 91.6743 1 TCGGAAG 142060 0.0 91.14019 3 ATCGGAA 141640 0.0 90.99803 2 CGGAAGA 142170 0.0 90.578636 4 GAAGAGC 143170 0.0 89.928474 6 GAGCACA 144350 0.0 88.99265 9 GGAAGAG 145600 0.0 88.45369 5 AGAGCAC 146115 0.0 88.011925 8 AAGAGCA 146400 0.0 87.70759 7 GCGCACA 1025 0.0 53.281647 9 CTCGGAA 1155 0.0 51.807983 2 GCTCGGA 1180 0.0 50.799404 1 AGCGCAC 1115 0.0 50.258656 8 TTGTGTT 24895 0.0 49.494587 1 CGGAAGC 1160 0.0 47.90005 4 GCCGTCT 138045 0.0 46.606342 50-51 ATGCCGT 137850 0.0 46.55571 48-49 GTATGCC 138330 0.0 46.490185 46-47 TCGTATG 138985 0.0 46.391224 44-45 TCTCGTA 136445 0.0 46.32702 42-43 >>END_MODULE