FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906452
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT118194924.089688434738584TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT161970.3301163447639965TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT107760.21962917399375353TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT102570.20905126555808556TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT79330.16168506285193454TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT75840.15457197991542565TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG63730.12989019356553372TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT53420.10887704597945726TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCGAAATCTCGTAT53260.10855094475600699TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT52740.10749111577979362TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1410600.091.67431
TCGGAAG1420600.091.140193
ATCGGAA1416400.090.998032
CGGAAGA1421700.090.5786364
GAAGAGC1431700.089.9284746
GAGCACA1443500.088.992659
GGAAGAG1456000.088.453695
AGAGCAC1461150.088.0119258
AAGAGCA1464000.087.707597
GCGCACA10250.053.2816479
CTCGGAA11550.051.8079832
GCTCGGA11800.050.7994041
AGCGCAC11150.050.2586568
TTGTGTT248950.049.4945871
CGGAAGC11600.047.900054
GCCGTCT1380450.046.60634250-51
ATGCCGT1378500.046.5557148-49
GTATGCC1383300.046.49018546-47
TCGTATG1389850.046.39122444-45
TCTCGTA1364450.046.3270242-43
Produced by FastQC (version 0.11.2)