##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_30521-1.341000000356df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3112678 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66445517332664 33.0 33.0 33.0 33.0 33.0 2 32.67566513465254 33.0 33.0 33.0 33.0 33.0 3 32.7313862853787 33.0 33.0 33.0 33.0 33.0 4 36.67573227940699 37.0 37.0 37.0 37.0 37.0 5 36.63047189590443 37.0 37.0 37.0 37.0 37.0 6 36.5298874474006 37.0 37.0 37.0 37.0 37.0 7 36.47671490594273 37.0 37.0 37.0 37.0 37.0 8 36.409051948193806 37.0 37.0 37.0 37.0 37.0 9 36.40979825089521 37.0 37.0 37.0 37.0 37.0 10-11 36.36133981735342 37.0 37.0 37.0 37.0 37.0 12-13 36.3649890865679 37.0 37.0 37.0 37.0 37.0 14-15 38.22987183383569 40.0 37.0 40.0 37.0 40.0 16-17 38.215819304149036 40.0 37.0 40.0 37.0 40.0 18-19 38.184889988620725 40.0 37.0 40.0 37.0 40.0 20-21 38.125304962479255 40.0 37.0 40.0 37.0 40.0 22-23 38.00216662308148 40.0 37.0 40.0 33.0 40.0 24-25 37.9935536216724 40.0 37.0 40.0 33.0 40.0 26-27 37.91396508087249 40.0 37.0 40.0 33.0 40.0 28-29 37.76703436719121 40.0 37.0 40.0 33.0 40.0 30-31 37.701147050867455 40.0 37.0 40.0 33.0 40.0 32-33 37.58390684805817 40.0 37.0 40.0 33.0 40.0 34-35 37.475370886419995 40.0 37.0 40.0 33.0 40.0 36-37 37.40324665770118 40.0 37.0 40.0 33.0 40.0 38-39 37.31433720416953 40.0 37.0 40.0 33.0 40.0 40-41 37.213677739875436 40.0 37.0 40.0 33.0 40.0 42-43 36.99770583401174 37.0 37.0 40.0 33.0 40.0 44-45 36.84334020415861 37.0 37.0 40.0 33.0 40.0 46-47 36.8177347608715 37.0 37.0 40.0 33.0 40.0 48-49 36.58858368902919 37.0 37.0 40.0 33.0 40.0 50-51 36.44298269849949 37.0 37.0 40.0 33.0 40.0 52-53 36.28779687458838 37.0 37.0 40.0 33.0 40.0 54-55 35.95995184853685 37.0 37.0 40.0 33.0 40.0 56-57 35.793362500072284 37.0 37.0 40.0 30.0 40.0 58-59 35.551375696426035 37.0 33.0 40.0 27.0 40.0 60-61 35.258659745723776 37.0 33.0 38.5 27.0 40.0 62-63 34.91089312803959 37.0 33.0 37.0 27.0 40.0 64-65 34.65498728104866 37.0 33.0 37.0 27.0 40.0 66-67 34.290705945170046 37.0 33.0 37.0 27.0 40.0 68-69 34.02637584099608 37.0 33.0 37.0 27.0 40.0 70-71 33.600940251449074 37.0 33.0 37.0 27.0 37.0 72-73 33.23185517422618 37.0 33.0 37.0 27.0 37.0 74-75 32.79255515668501 37.0 33.0 37.0 24.5 37.0 76-77 30.062848453967934 33.0 30.0 33.0 22.0 37.0 78-79 32.461407990161526 33.0 33.0 37.0 27.0 37.0 80-81 32.90672228222772 37.0 33.0 37.0 27.0 37.0 82-83 32.905210400818845 37.0 33.0 37.0 27.0 37.0 84-85 32.79124760094041 37.0 33.0 37.0 27.0 37.0 86-87 32.66641361554263 37.0 33.0 37.0 27.0 37.0 88-89 32.46800905843779 33.0 33.0 37.0 22.0 37.0 90-91 32.22189542252684 33.0 33.0 37.0 22.0 37.0 92-93 32.219575715830544 33.0 33.0 37.0 22.0 37.0 94-95 32.16161308686603 33.0 33.0 37.0 22.0 37.0 96-97 31.97846083019188 33.0 33.0 37.0 22.0 37.0 98-99 31.811162767237732 33.0 33.0 37.0 22.0 37.0 100-101 30.08907747605117 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.09519657357758149 2306 2 0.10532660549388595 2306 3 0.049753910848181704 2306 4 0.14286927770147173 2306 5 0.17023893629217213 2306 6 0.1447233761264357 2306 7 0.14530243450739988 2306 8 0.13414559169108742 2306 9 0.210913852946625 2306 10-11 0.18800561879175604 2306 12-13 0.21731852536590424 2306 14-15 0.3632335673434639 2306 16-17 0.3696335677530058 2306 18-19 0.34814599832688486 2306 20-21 0.39035450160353236 2306 22-23 0.37400405332192577 2306 24-25 0.378907256768926 2306 26-27 0.32840693115073094 2306 28-29 0.3033455997583374 2306 30-31 0.3593958046333867 2306 32-33 0.4690022476752844 2306 34-35 0.34755341687237973 2306 36-37 0.3924329417821326 2306 38-39 0.3677916929824576 2306 40-41 0.2505027028146145 2306 42-43 0.21741557988598004 2306 44-45 0.2857901668792806 2306 46-47 0.3726136671635629 2306 48-49 0.3205309347804999 2306 50-51 0.10991048647023405 2306 52-53 0.001700026165003976 2306 54-55 0.15824512606345564 2306 56-57 0.32820876144182165 2306 58-59 0.3217865540518261 2306 60-61 0.17250439526797123 2306 62-63 0.20487502954127734 2306 64-65 0.17796445921540993 2306 66-67 0.11820895134913911 2306 68-69 0.2236033964464923 2306 70-71 0.17002073936429696 2306 72-73 0.10116520589174627 2306 74-75 -0.017152709333522864 2306 76-77 -0.20330110401433288 2306 78-79 0.2047154858897997 2306 80-81 0.3009854306130464 2306 82-83 0.26263345719327447 2306 84-85 0.4041333125354214 2306 86-87 0.24893839339889468 2306 88-89 0.38377367600109125 2306 90-91 0.3996462466917663 2306 92-93 0.30712159919048077 2306 94-95 0.2578714209595745 2306 96-97 0.265742124480596 2306 98-99 0.38393418812269786 2306 100-101 0.16471078115689863 2307 1 0.12449166054737049 2307 2 0.10040247865946839 2307 3 0.11345546714967725 2307 4 0.11928201662565385 2307 5 0.13923427571015878 2307 6 0.14096786249145765 2307 7 0.17607129183255665 2307 8 0.19805174199485975 2307 9 0.20025069096365655 2307 10-11 0.20329641969058088 2307 12-13 0.23806574427455018 2307 14-15 0.3473757983196961 2307 16-17 0.3384936670677945 2307 18-19 0.37309779736703064 2307 20-21 0.3216143845341506 2307 22-23 0.34720744056635766 2307 24-25 0.47294594237382626 2307 26-27 0.4363684119951827 2307 28-29 0.45650154893580464 2307 30-31 0.4363647618281661 2307 32-33 0.38890196193721494 2307 34-35 0.40981414592057064 2307 36-37 0.3768716606769047 2307 38-39 0.40249465023271114 2307 40-41 0.4273846208660501 2307 42-43 0.37895641546175796 2307 44-45 0.4975536284710884 2307 46-47 0.4690051837946285 2307 48-49 0.4259107167318419 2307 50-51 0.3638619248701005 2307 52-53 0.3055793365098225 2307 54-55 0.579457362865412 2307 56-57 0.4589061842509352 2307 58-59 0.5488928319667679 2307 60-61 0.40624610747703827 2307 62-63 0.26132216799940267 2307 64-65 0.3159856537669654 2307 66-67 0.2382917599046337 2307 68-69 0.25309614202865305 2307 70-71 0.33525987160830795 2307 72-73 0.17917447157265087 2307 74-75 0.13107217761962175 2307 76-77 -0.028312547407679745 2307 78-79 0.28142436596304066 2307 80-81 0.46210320740446065 2307 82-83 0.4501282556508386 2307 84-85 0.49083782331301506 2307 86-87 0.28413896487502655 2307 88-89 0.48390510163984146 2307 90-91 0.5414697687601873 2307 92-93 0.3900012424340247 2307 94-95 0.4260199492698362 2307 96-97 0.3174229162247144 2307 98-99 0.1901975387482686 2307 100-101 0.2135636676661754 2201 1 0.11360387338466182 2201 2 0.10742211075282881 2201 3 0.05842168260109304 2201 4 0.08613759319106151 2201 5 0.12397835671205115 2201 6 0.11464096555845771 2201 7 0.0870270234101227 2201 8 0.14733813202261103 2201 9 0.11371151911701105 2201 10-11 0.12569613838104488 2201 12-13 0.06886017265381383 2201 14-15 0.25988820465410356 2201 16-17 0.21896779457333793 2201 18-19 0.24343678369950794 2201 20-21 0.2786784887938225 2201 22-23 0.22046571334247744 2201 24-25 0.24544363089351862 2201 26-27 0.18093486943713089 2201 28-29 0.2337547507530573 2201 30-31 0.20856361109223798 2201 32-33 0.23810542463004225 2201 34-35 0.19956244775701037 2201 36-37 0.18730413685614877 2201 38-39 0.06022331997579755 2201 40-41 0.22271562425105884 2201 42-43 0.2631066455970199 2201 44-45 0.18760161849597523 2201 46-47 0.23465607996984517 2201 48-49 0.2694254357380501 2201 50-51 0.19999427916045676 2201 52-53 0.2137003025894444 2201 54-55 0.307726692619525 2201 56-57 0.33559317764653684 2201 58-59 0.29372029902519614 2201 60-61 0.25712070192543734 2201 62-63 0.24685813951814595 2201 64-65 0.2456156739388078 2201 66-67 0.2730898151633099 2201 68-69 0.2666104082706795 2201 70-71 0.39506242472172204 2201 72-73 0.08970826863183845 2201 74-75 0.02996405217057685 2201 76-77 -0.1270409800453045 2201 78-79 0.26971758200662066 2201 80-81 0.19772950835680803 2201 82-83 0.0898916446622593 2201 84-85 0.18412039641953726 2201 86-87 0.2452246278588106 2201 88-89 0.3917043668512292 2201 90-91 0.45350223016501445 2201 92-93 0.3018913214175427 2201 94-95 0.09612673012839679 2201 96-97 0.18072410032220176 2201 98-99 0.10086225018854478 2201 100-101 0.1503598725491173 1101 1 0.16920179375001965 1101 2 0.18001384773778284 1101 3 0.12088241987862602 1101 4 0.11943421662868303 1101 5 0.17743691298990427 1101 6 0.11428586329931534 1101 7 0.20834511331563021 1101 8 0.15794645917058858 1101 9 0.12907449050432263 1101 10-11 0.1151825851468331 1101 12-13 0.1773299401987316 1101 14-15 0.30520664853277424 1101 16-17 0.2822713351658592 1101 18-19 0.2158030956732162 1101 20-21 0.27359033635164565 1101 22-23 0.3126992910273785 1101 24-25 0.3850609898613939 1101 26-27 0.29062752391543967 1101 28-29 0.25110451645754495 1101 30-31 0.37930111021542956 1101 32-33 0.28894453719619406 1101 34-35 0.26274376316811754 1101 36-37 0.31150503327764767 1101 38-39 0.3640323562556418 1101 40-41 0.3395936656786134 1101 42-43 0.3799227981769491 1101 44-45 0.4010554071449235 1101 46-47 0.45081117215176647 1101 48-49 0.4118470581347822 1101 50-51 0.3630128205511127 1101 52-53 0.273600238837588 1101 54-55 0.46899893820237537 1101 56-57 0.4244894856698025 1101 58-59 0.43600000754213397 1101 60-61 0.45425524122330074 1101 62-63 0.4225971325830713 1101 64-65 0.4445457660748531 1101 66-67 0.3524887608370406 1101 68-69 0.37984716959693543 1101 70-71 0.5118687682368588 1101 72-73 0.3271466320677092 1101 74-75 0.378184087982838 1101 76-77 0.13977496788507082 1101 78-79 0.5129981256348728 1101 80-81 0.5615077794576209 1101 82-83 0.5661659162756791 1101 84-85 0.5550654243033932 1101 86-87 0.5114860460689314 1101 88-89 0.49653137234980704 1101 90-91 0.564128580587429 1101 92-93 0.40576303691571525 1101 94-95 0.4242275978001615 1101 96-97 0.43522811942771966 1101 98-99 0.39001562627810316 1101 100-101 0.3753225875175943 2304 1 0.1995895043566165 2304 2 0.1775029539566333 2304 3 0.11219356937595393 2304 4 0.1161268536977147 2304 5 0.18626417066267464 2304 6 0.11087300951663792 2304 7 0.1884195041951955 2304 8 0.1279025852874156 2304 9 0.14057391869896207 2304 10-11 0.09780931264214843 2304 12-13 0.1167616009644803 2304 14-15 0.2577555282741244 2304 16-17 0.26522352441168806 2304 18-19 0.24352108134787187 2304 20-21 0.33709655614778455 2304 22-23 0.342547583813932 2304 24-25 0.42570603337108537 2304 26-27 0.3628536598159471 2304 28-29 0.2918610656482059 2304 30-31 0.2234162343119266 2304 32-33 0.38705598656249407 2304 34-35 0.4613719326361334 2304 36-37 0.3676931887701116 2304 38-39 0.3551915486927655 2304 40-41 0.3468842336199529 2304 42-43 0.44429436022282687 2304 44-45 0.41752460454329565 2304 46-47 0.3714790112033981 2304 48-49 0.46668524799108724 2304 50-51 0.3850307970911615 2304 52-53 0.347746823795994 2304 54-55 0.5294764601244424 2304 56-57 0.5087451479044844 2304 58-59 0.4950165208262405 2304 60-61 0.4007904153499666 2304 62-63 0.4034124711115439 2304 64-65 0.42096756926250123 2304 66-67 0.32458444764924366 2304 68-69 0.2681693058739256 2304 70-71 0.43337584121339745 2304 72-73 0.18366909309260393 2304 74-75 0.3761592717226492 2304 76-77 0.27883352416477436 2304 78-79 0.34085701694717585 2304 80-81 0.4219372356231972 2304 82-83 0.4214180585794054 2304 84-85 0.4152322769891157 2304 86-87 0.36379211177968074 2304 88-89 0.4461620684426961 2304 90-91 0.4570001089899236 2304 92-93 0.1802909488300486 2304 94-95 0.27387603433452057 2304 96-97 0.21771074218939646 2304 98-99 0.07484465376481708 2304 100-101 0.13999444156690544 2202 1 0.11500551324851926 2202 2 0.1455170805274406 2202 3 0.08200369288082499 2202 4 0.062248411696046446 2202 5 0.16011525672710292 2202 6 0.07176325518008753 2202 7 0.07170511449818662 2202 8 0.09473228969131497 2202 9 0.1440844262766987 2202 10-11 0.10932313000486005 2202 12-13 0.10888314723463566 2202 14-15 0.11489876064835158 2202 16-17 0.1544147528217934 2202 18-19 0.16703886771598775 2202 20-21 0.1731491330081738 2202 22-23 0.22358725114462885 2202 24-25 0.17990098913759311 2202 26-27 0.20389608082128063 2202 28-29 0.15430286159138973 2202 30-31 0.1134764462056026 2202 32-33 0.21125481262237855 2202 34-35 0.22990639864925555 2202 36-37 0.25979269586967746 2202 38-39 0.25906118235630515 2202 40-41 0.2969677112544673 2202 42-43 0.3375875131998356 2202 44-45 0.19585813293876697 2202 46-47 0.18745847274882976 2202 48-49 0.2697504763616081 2202 50-51 0.25435934517161485 2202 52-53 0.2238563873055739 2202 54-55 0.37220486546339515 2202 56-57 0.3186730001628746 2202 58-59 0.25956964243530933 2202 60-61 0.2862956413455322 2202 62-63 0.13805926896910137 2202 64-65 0.26475798408024787 2202 66-67 0.2576827983032004 2202 68-69 0.31894057911004836 2202 70-71 0.34265875452786787 2202 72-73 0.1503846922562886 2202 74-75 0.2278220318526678 2202 76-77 0.028127635430333697 2202 78-79 0.49664334987596703 2202 80-81 0.526411578192544 2202 82-83 0.42706077039427726 2202 84-85 0.25511666419144774 2202 86-87 0.3654970932919426 2202 88-89 0.49999500273347053 2202 90-91 0.5029489698066882 2202 92-93 0.3105210064959607 2202 94-95 0.42009890654667714 2202 96-97 0.2956273955193467 2202 98-99 0.17959662391273312 2202 100-101 0.20998780538205608 1102 1 0.15526878040494552 1102 2 0.19501183316665305 1102 3 0.10544217114149035 1102 4 0.10699761449451017 1102 5 0.15716958882426724 1102 6 0.08128471771891554 1102 7 0.13235668729419814 1102 8 0.11597028459411973 1102 9 0.11042684587161489 1102 10-11 0.07640355532964804 1102 12-13 0.1087075604783081 1102 14-15 0.19092570780191664 1102 16-17 0.10559045443916659 1102 18-19 0.15714704892905473 1102 20-21 0.16141565393312618 1102 22-23 0.22510492326907894 1102 24-25 0.16362320241570671 1102 26-27 0.15267123374180613 1102 28-29 0.15842526674393298 1102 30-31 0.13156011682556112 1102 32-33 0.08068569969796613 1102 34-35 0.06750724334865765 1102 36-37 0.09330832403247058 1102 38-39 0.06691584171328913 1102 40-41 0.1263036214293649 1102 42-43 0.12862879915392966 1102 44-45 0.15719844831705387 1102 46-47 0.1253321830335068 1102 48-49 0.045343658323147906 1102 50-51 0.016689960440793072 1102 52-53 0.11148615909927884 1102 54-55 0.06810743317257106 1102 56-57 0.24446558842463872 1102 58-59 0.13583935122490942 1102 60-61 -0.048165236701230185 1102 62-63 0.08326713921001527 1102 64-65 0.10837420509549389 1102 66-67 0.11253009902164735 1102 68-69 0.09573603607340431 1102 70-71 0.11649065539337045 1102 72-73 0.08714250869140017 1102 74-75 0.1758008070385415 1102 76-77 -0.10469444858204469 1102 78-79 0.23891164832260614 1102 80-81 0.4290209480290983 1102 82-83 0.36969321129497956 1102 84-85 0.1372414616547104 1102 86-87 0.15828753873623924 1102 88-89 0.2382974177339463 1102 90-91 0.28120078475237875 1102 92-93 0.18459388017799938 1102 94-95 0.032310612393196436 1102 96-97 0.03013727780560771 1102 98-99 -0.023976448893154867 1102 100-101 0.16344401649288542 2305 1 0.09812534310800203 2305 2 0.10919537587115258 2305 3 0.08260762850918724 2305 4 0.038822933966031314 2305 5 0.11302882281459148 2305 6 0.05602932099665736 2305 7 0.16189561950125864 2305 8 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24.0 11 33.0 12 38.0 13 99.0 14 277.0 15 711.0 16 1373.0 17 2289.0 18 3269.0 19 4531.0 20 6021.0 21 7904.0 22 10258.0 23 13083.0 24 16990.0 25 21725.0 26 27919.0 27 37163.0 28 53008.0 29 72663.0 30 72871.0 31 78515.0 32 103164.0 33 147108.0 34 228036.0 35 399802.0 36 736541.0 37 898373.0 38 168858.0 39 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.94044028999071 25.55322686437083 18.275587728112143 16.23074511752631 2 32.85174373963513 23.68625987011827 28.124753026172318 15.33724336407428 3 38.69610027121341 18.905842493184323 24.159646452347463 18.238410783254803 4 33.29150043551838 18.587461761614815 27.5125201493518 20.60851765351501 5 36.79744580068996 17.086348154226037 26.959614839697522 19.156591205386487 6 32.940895267676254 17.8517341016321 29.729512657589378 19.47785797310226 7 34.962048756729736 20.222875607435142 23.27709451475546 21.53798112107966 8 31.723101457972845 25.794283893162095 21.718019017707583 20.76459563115748 9 31.80168330935612 22.16387303794353 24.386942690506373 21.64750096219397 10-11 24.8646990174605 28.636789690925347 28.675310572600537 17.82320071901362 12-13 23.790575189595582 27.44594847266566 28.18531181188674 20.578164525852017 14-15 24.0473637170308 27.57056785186261 27.844238947941292 20.5378294831653 16-17 24.9532314250127 26.129650232421263 28.234276265337733 20.682842077228305 18-19 23.23230992733588 26.055714725390803 29.26910525277591 21.442870094497408 20-21 23.88849730039535 26.474148627002215 29.000767185041305 20.63658688756113 22-23 22.56328794690617 28.955838027576252 27.651077303852183 20.829796721665396 24-25 22.66341073506479 25.684892558754875 29.515741750351303 22.135954955829032 26-27 22.364504134382035 25.79454090657627 28.68648796952335 23.154466989518347 28-29 23.355997632906455 27.903801806675794 27.743746702999793 20.996453857417954 30-31 21.516151044213373 27.78313400872175 28.86854984678788 21.832165100276995 32-33 21.29155344690328 28.871698261111494 27.80090006097643 22.0358482310088 34-35 21.633557984475104 28.725891338583686 29.24383762149506 20.39671305544615 36-37 21.130759429661534 29.17243608237023 29.63411249091618 20.062691997052056 38-39 21.267748397242826 27.698888872825172 29.122085841280853 21.911276888651145 40-41 22.703114038547206 27.27864481884883 27.66965266647593 22.34858847612804 42-43 21.924029322917544 28.260937661893298 28.572463541127025 21.242569474062133 44-45 21.421591817228958 27.077872061876786 28.75609511226412 22.74444100863014 46-47 22.57687206581066 27.145757481901683 28.13722913568887 22.14014131659879 48-49 21.294916805172097 28.76060317575576 28.926922146399892 21.01755787267225 50-51 22.66304920552508 27.557593648313123 27.67630866031635 22.103048485845445 52-53 21.497594746273645 26.947564745864 27.26647673100757 24.288363776854784 54-55 23.014115128575977 26.87094459570232 28.822005166367777 21.292935109353927 56-57 22.148206989008898 27.129630343717746 27.968407170758606 22.75375549651475 58-59 21.93663247655874 27.612988438242898 27.99452401247844 22.455855072719924 60-61 22.94218155443731 27.337413037421427 28.17781965385608 21.542585754285184 62-63 21.75274602049531 27.134853013280757 28.47912266147269 22.633278304751244 64-65 23.162735699384427 27.33173797193096 28.49554849183723 21.009977836847384 66-67 22.28878543339506 29.54768169092158 27.18002193185059 20.983510943832773 68-69 22.561585995442492 29.214531714413617 27.134970998979043 21.08891129116485 70-71 22.095918349966954 29.477769445478618 27.149281932384973 21.277030272169455 72-73 21.960371105351587 29.73764169788909 26.899819775179296 21.40216742158003 74-75 22.396567058772487 28.951630462291718 26.838340509397447 21.813461969538352 76-77 22.1251070351477 28.356484847647344 26.787187693974847 22.73122042323011 78-79 21.81674221885316 28.39026445246864 27.156707777724577 22.636285550953623 80-81 21.34497850828406 28.41756518039997 27.709260683686537 22.528195627629433 82-83 20.80634103275187 28.44910840270692 27.71535261657718 23.02919794796403 84-85 20.833386212829396 29.11251605461662 27.434723274234134 22.619374458319847 86-87 20.63384025964448 29.12900550759554 27.681512285439247 22.555641947320733 88-89 21.000654752579845 28.973966555398896 27.611968716484096 22.413409975537157 90-91 21.450256148240623 28.816239244143777 26.836221779308428 22.897282828307173 92-93 21.46132113677417 28.879778610595064 26.758965820921425 22.89993443170934 94-95 21.771917424495907 29.091835950821377 26.65419876452379 22.48204786015893 96-97 21.782995513193192 29.814881055108245 26.46319362559122 21.93892980610734 98-99 21.920551842149543 29.465441486415187 26.660349957068124 21.953656714367145 100-101 22.000606490801474 29.522088783889522 26.361233570792535 22.11607115451647 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26.0 1 17.5 2 22.5 3 51.5 4 50.5 5 27.5 6 19.0 7 25.0 8 31.5 9 33.5 10 38.5 11 37.0 12 37.5 13 40.0 14 41.5 15 54.0 16 72.5 17 93.0 18 127.5 19 187.0 20 251.5 21 343.5 22 479.5 23 663.0 24 956.0 25 1383.0 26 1933.5 27 2676.5 28 3546.0 29 4598.0 30 6134.5 31 8341.5 32 12993.5 33 21726.0 34 35718.5 35 55620.5 36 85312.5 37 120651.5 38 143992.5 39 149406.5 40 152553.0 41 156836.5 42 164941.5 43 178841.0 44 177884.0 45 167765.5 46 164317.0 47 160007.5 48 150124.0 49 134494.0 50 120343.5 51 116305.5 52 112470.5 53 101932.5 54 87946.0 55 72547.5 56 58868.5 57 45325.5 58 32287.5 59 23432.5 60 17654.5 61 14066.0 62 11260.5 63 8562.0 64 6364.5 65 4654.0 66 3371.0 67 2490.5 68 1944.5 69 1445.5 70 984.0 71 748.5 72 569.5 73 387.5 74 286.0 75 227.0 76 181.5 77 134.0 78 92.5 79 66.5 80 53.5 81 44.0 82 32.0 83 20.0 84 17.5 85 15.5 86 9.0 87 7.5 88 6.5 89 3.0 90 3.0 91 2.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1781102960216251 2 0.0 3 0.0 4 0.009477369647615332 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0025861974801119807 12-13 0.0 14-15 0.0 16-17 3.533934444873514E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.00234524740432515 40-41 0.006698412106873887 42-43 0.003068097631685642 44-45 0.0013011304092488847 46-47 0.007517642364549111 48-49 0.009156102879899559 50-51 0.005847055172427087 52-53 0.007083932228132818 54-55 0.020143426335779032 56-57 0.022681433800733643 58-59 0.029251339200521226 60-61 0.027339801932612366 62-63 0.011437096930681555 64-65 0.017043202027321812 66-67 0.0245929710686425 68-69 0.028624869003475467 70-71 0.03079341968555694 72-73 0.02350066405840887 74-75 0.034070340716257835 76-77 0.031050433099729557 78-79 0.02252080041687576 80-81 0.010152029859818458 82-83 0.0019918539598377987 84-85 0.008208365915138025 86-87 0.02841604560446021 88-89 0.02619930490722137 90-91 0.022826003846205742 92-93 0.045555627662096754 94-95 0.047836621712878745 96-97 0.0356927378932225 98-99 0.04348345701033001 100-101 0.042937303505213194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3112678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.995412232887965 #Duplication Level Percentage of deduplicated Percentage of total 1 85.25419185153677 40.065558906446896 2 6.942910675587097 6.525698985906686 3 2.5481196320419937 3.592497975795849 4 1.2272560076525594 2.3070160797968136 5 0.7446945909627493 1.7498614644948147 6 0.5402190045180439 1.5232688888019508 7 0.3207536893199244 1.0551766298366665 8 0.28105533221053874 1.0566648957988434 9 0.20938908104385362 0.885629356264934 >10 1.5465219994468555 14.578960750128944 >50 0.21526288138500738 6.96184312193516 >100 0.1617949109312607 14.47958653748158 >500 0.006065197565537906 1.9518158492912447 >1k 0.0016967774060875779 1.1710942466897267 >5k 0.0 0.0 >10k+ 6.836839173767446E-5 2.09532631132983 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 65214 2.0951090989816485 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4004 0.1286352137933959 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3727 0.11973612432766897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001285067070863096 0.0 0.0 2 0.001445700454720983 0.0 0.0 3 0.0016063338385788701 0.0 0.0 4 0.0017990938992083344 0.0 0.0 5 0.0020561073133809537 0.0 0.0 6 0.002248867374010418 0.0 0.0 7 0.0025058807881830373 0.0 0.0 8 0.002730767525584079 0.0 0.0 9 0.002955654262985121 0.0 0.0 10-11 0.004963571561208708 0.0 0.0 12-13 0.006859045490731776 0.0 0.0 14-15 0.008561759359625377 0.0 0.0 16-17 0.010103839844661093 0.0 0.0 18-19 0.01233664388028572 0.0 0.0 20-21 0.015195918112956109 0.0 0.0 22-23 0.018392522451728063 0.0 0.0 24-25 0.021653380144043166 0.0 0.0 26-27 0.025524644695018243 0.0 0.0 28-29 0.030472152917841167 0.0 0.0 30-31 0.06462281032602793 0.0 0.0 32-33 0.07186737593801865 0.0 0.0 34-35 0.07895130816615145 0.0 0.0 36-37 0.08669383726810162 0.0 0.0 38-39 0.0956732434257575 0.0 0.0 40-41 0.10529518311884493 0.0 0.0 42-43 0.11649132997373965 0.0 0.0 44-45 0.13106077788965 0.0 0.0 46-47 0.14577479585103245 0.0 0.0 48-49 0.1623682244035522 0.0 0.0 50-51 0.18106595028461023 0.0 0.0 52-53 0.20202860687806448 0.0 0.0 54-55 0.2253204475374581 0.0 0.0 56-57 0.2526120594549131 0.0 0.0 58-59 0.28239348882216536 0.0 0.0 60-61 0.3137651886896107 0.0 0.0 62-63 0.348558379633229 0.0 0.0 64-65 0.3856646913044009 0.0 0.0 66-67 0.4270277876478068 0.0 0.0 68-69 0.47125015822388305 0.0 0.0 70-71 0.5208698104975844 0.0 0.0 72-73 0.5752442109334792 0.0 0.0 74-75 0.6381161173754561 0.0 0.0 76-77 0.7095658465154443 0.0 0.0 78-79 0.7902519952272609 0.0 0.0 80-81 0.8786003563490987 0.0 0.0 82-83 0.9757996169215062 0.0 0.0 84-85 1.0916162866830428 0.0 0.0 86-87 1.2246046651789873 0.0 0.0 88-89 1.3703794610300197 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 12940 0.0 54.65376 4 TCGGAAG 13050 0.0 54.375053 3 GAAGAGC 13130 0.0 53.931763 6 ATCGGAA 13570 0.0 52.116405 2 GATCGGA 13625 0.0 52.10687 1 GGAAGAG 15480 0.0 45.89785 5 ATGCCGT 8150 0.0 43.389103 48-49 GCCGTCT 8295 0.0 42.9473 50-51 TATGCCG 8135 0.0 42.856045 48-49 GTATGCC 8280 0.0 42.56241 46-47 TCGTATG 8370 0.0 42.331387 44-45 TGCCGTC 8410 0.0 41.908188 50-51 GATCTCG 8050 0.0 41.889786 40-41 TCGATCT 8105 0.0 41.576893 38-39 TCTCGTA 8170 0.0 41.303246 42-43 CGTATGC 8530 0.0 41.120094 46-47 CTCGATC 8100 0.0 40.986874 38-39 TTACTCG 8685 0.0 40.9051 34-35 GAGCACA 17365 0.0 40.86086 9 TACTCGA 8525 0.0 40.809933 36-37 >>END_MODULE