FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT652142.0951090989816485TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40040.1286352137933959No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA37270.11973612432766897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA129400.054.653764
TCGGAAG130500.054.3750533
GAAGAGC131300.053.9317636
ATCGGAA135700.052.1164052
GATCGGA136250.052.106871
GGAAGAG154800.045.897855
ATGCCGT81500.043.38910348-49
GCCGTCT82950.042.947350-51
TATGCCG81350.042.85604548-49
GTATGCC82800.042.5624146-47
TCGTATG83700.042.33138744-45
TGCCGTC84100.041.90818850-51
GATCTCG80500.041.88978640-41
TCGATCT81050.041.57689338-39
TCTCGTA81700.041.30324642-43
CGTATGC85300.041.12009446-47
CTCGATC81000.040.98687438-39
TTACTCG86850.040.905134-35
GAGCACA173650.040.860869
TACTCGA85250.040.80993336-37
Produced by FastQC (version 0.11.2)