FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8534077
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3079513.6084863073065785TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG471500.071.9322363
CGGAAGA471900.071.7303544
GATCGGA484500.069.9830251
GAAGAGC485500.069.725086
ATCGGAA500150.067.6313252
GGAAGAG552200.061.5094345
AGAGCAC764050.044.379978
GAGCACA767350.044.170559
AAGAGCA767400.044.093417
GCCGTCT384800.044.03874650-51
ATGCGCG652.5633271E-643.915481
CGTCTTC386200.043.83183352-53
ATGCCGT385150.043.80640848-49
TGCCGTC385500.043.51518650-51
CCGTCTT385250.043.50834752-53
TCTCGTA384450.043.44927642-43
TCGTATG390900.043.34125544-45
TATGCCG385300.043.32088548-49
CGTATGC388200.043.08713546-47
CTGCTTG395500.042.9903458-59
Produced by FastQC (version 0.11.2)