##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8534077 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66388433101787 33.0 33.0 33.0 33.0 33.0 2 32.67567552999581 33.0 33.0 33.0 33.0 33.0 3 32.726114259339354 33.0 33.0 33.0 33.0 33.0 4 36.663102172619254 37.0 37.0 37.0 37.0 37.0 5 36.61980739100432 37.0 37.0 37.0 37.0 37.0 6 36.51865105037135 37.0 37.0 37.0 37.0 37.0 7 36.449324513945676 37.0 37.0 37.0 37.0 37.0 8 36.40196157123963 37.0 37.0 37.0 37.0 37.0 9 36.417727189478136 37.0 37.0 37.0 37.0 37.0 10-11 36.36790692186162 37.0 37.0 37.0 37.0 37.0 12-13 36.350951251084325 37.0 37.0 37.0 37.0 37.0 14-15 38.18022487962084 40.0 37.0 40.0 37.0 40.0 16-17 38.18276903290186 40.0 37.0 40.0 37.0 40.0 18-19 38.14787433954486 40.0 37.0 40.0 37.0 40.0 20-21 38.09252892843597 40.0 37.0 40.0 37.0 40.0 22-23 37.92842383540716 40.0 37.0 40.0 33.0 40.0 24-25 37.94384020673823 40.0 37.0 40.0 33.0 40.0 26-27 37.8644827085577 40.0 37.0 40.0 33.0 40.0 28-29 37.69922722750216 40.0 37.0 40.0 33.0 40.0 30-31 37.65471134136709 40.0 37.0 40.0 33.0 40.0 32-33 37.50172168589526 40.0 37.0 40.0 33.0 40.0 34-35 37.38844353056575 40.0 37.0 40.0 33.0 40.0 36-37 37.23632297904038 40.0 37.0 40.0 33.0 40.0 38-39 37.19958414952197 40.0 37.0 40.0 33.0 40.0 40-41 37.15778695223865 40.0 37.0 40.0 33.0 40.0 42-43 36.92220875204196 38.5 37.0 40.0 33.0 40.0 44-45 36.805605691160274 37.0 37.0 40.0 33.0 40.0 46-47 36.803993800384035 37.0 37.0 40.0 33.0 40.0 48-49 36.52582751479744 37.0 37.0 40.0 33.0 40.0 50-51 36.42560701057654 37.0 37.0 40.0 33.0 40.0 52-53 36.31176335765426 37.0 37.0 40.0 33.0 40.0 54-55 35.98342257750897 37.0 37.0 40.0 30.0 40.0 56-57 35.84304670557812 37.0 37.0 40.0 27.0 40.0 58-59 35.65185795722256 37.0 35.0 40.0 27.0 40.0 60-61 35.311399463585815 37.0 33.0 40.0 27.0 40.0 62-63 35.0109581270476 37.0 33.0 38.5 27.0 40.0 64-65 34.77306989379167 37.0 33.0 37.0 27.0 40.0 66-67 34.309674672492406 37.0 33.0 37.0 27.0 40.0 68-69 34.06435136453538 37.0 33.0 37.0 27.0 40.0 70-71 33.620828239538966 37.0 33.0 37.0 27.0 38.5 72-73 33.128168342048006 37.0 33.0 37.0 24.5 37.0 74-75 32.524582857642365 37.0 33.0 37.0 22.0 37.0 76-77 29.971497503479288 33.0 27.0 35.0 22.0 37.0 78-79 32.12481713019463 33.0 33.0 37.0 22.0 37.0 80-81 32.45772120406225 37.0 33.0 37.0 22.0 37.0 82-83 32.41499315040162 37.0 33.0 37.0 22.0 37.0 84-85 32.260637852224676 35.0 33.0 37.0 22.0 37.0 86-87 32.10943708382289 33.0 33.0 37.0 22.0 37.0 88-89 31.89158353035718 33.0 33.0 37.0 22.0 37.0 90-91 31.632912088794136 33.0 33.0 37.0 15.0 37.0 92-93 31.620507466712567 33.0 33.0 37.0 15.0 37.0 94-95 31.545275663671653 33.0 33.0 37.0 15.0 37.0 96-97 31.370826511173966 33.0 33.0 37.0 15.0 37.0 98-99 31.209910105099823 33.0 33.0 37.0 15.0 37.0 100-101 29.58340837562164 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1533436839647706 2306 2 0.15143307856492783 2306 3 0.11780802353528941 2306 4 0.14265730549001177 2306 5 0.19119317840259242 2306 6 0.17767421544054685 2306 7 0.21375910451416757 2306 8 0.1527925645216257 2306 9 0.20873088969772624 2306 10-11 0.15038438895759754 2306 12-13 0.1708224782557508 2306 14-15 0.39086801329739274 2306 16-17 0.35116907914234474 2306 18-19 0.36378151756873933 2306 20-21 0.3921846727930003 2306 22-23 0.37002711539651045 2306 24-25 0.3131934863486876 2306 26-27 0.3581171877289364 2306 28-29 0.42156271961953706 2306 30-31 0.41572359937316605 2306 32-33 0.3960336501602342 2306 34-35 0.34544333576644703 2306 36-37 0.38716651966496585 2306 38-39 0.332706992762958 2306 40-41 0.2634697982414025 2306 42-43 0.2898833784885113 2306 44-45 0.3734533744017341 2306 46-47 0.3271907665350682 2306 48-49 0.44993128348074407 2306 50-51 0.28628150971715627 2306 52-53 0.22450175162462926 2306 54-55 0.3781038554727516 2306 56-57 0.3185642216246478 2306 58-59 0.32003118405861386 2306 60-61 0.38112464290760073 2306 62-63 0.3428753473409998 2306 64-65 0.2231368267171021 2306 66-67 0.2271536584209244 2306 68-69 0.2891541555971955 2306 70-71 0.34455620780841656 2306 72-73 0.22975784437188906 2306 74-75 0.3157692376172818 2306 76-77 -0.12694530736709453 2306 78-79 0.3636900888507526 2306 80-81 0.32653509257281854 2306 82-83 0.33972839751525186 2306 84-85 0.3448494345525859 2306 86-87 0.28330246720129537 2306 88-89 0.4666493500357234 2306 90-91 0.49603124756509587 2306 92-93 0.34056737386879377 2306 94-95 0.31853157289939205 2306 96-97 0.30948276311361766 2306 98-99 0.10773739609426869 2306 100-101 0.17350363894096077 2307 1 0.15038186818215848 2307 2 0.1477199832231193 2307 3 0.13222027484481202 2307 4 0.1454496701706134 2307 5 0.21125390275739875 2307 6 0.2018291585212424 2307 7 0.12731499701533977 2307 8 0.20088048055214358 2307 9 0.18783578214389962 2307 10-11 0.2118202221349179 2307 12-13 0.21442212837604302 2307 14-15 0.36389053283910755 2307 16-17 0.3302277342474085 2307 18-19 0.3422588848536705 2307 20-21 0.3096514050775525 2307 22-23 0.3774441152500714 2307 24-25 0.3690497905784511 2307 26-27 0.3943627578518303 2307 28-29 0.42401391656939325 2307 30-31 0.3858259836752893 2307 32-33 0.38109427771525617 2307 34-35 0.4665112566699108 2307 36-37 0.37865457529311897 2307 38-39 0.36117022110148156 2307 40-41 0.4208987024081807 2307 42-43 0.4216084658812065 2307 44-45 0.4301390931184699 2307 46-47 0.4806616925155751 2307 48-49 0.6033225813454095 2307 50-51 0.4053640568612238 2307 52-53 0.2439405631457774 2307 54-55 0.4576054365014741 2307 56-57 0.4440020346663758 2307 58-59 0.499538315627845 2307 60-61 0.4061583786602725 2307 62-63 0.2998285629781563 2307 64-65 0.38258055649084355 2307 66-67 0.4020615132698637 2307 68-69 0.3102566103366087 2307 70-71 0.3732113880485741 2307 72-73 0.28192764745436705 2307 74-75 0.30371030220477024 2307 76-77 0.15761846957902748 2307 78-79 0.5100499898101631 2307 80-81 0.6073870614082324 2307 82-83 0.46575285325849336 2307 84-85 0.41608076172079933 2307 86-87 0.5065498402715534 2307 88-89 0.7092048549258649 2307 90-91 0.686838234367162 2307 92-93 0.5470335527469601 2307 94-95 0.5474463670567218 2307 96-97 0.39095754069553124 2307 98-99 0.3824686067938181 2307 100-101 0.39656158308254774 2201 1 0.12034486157858026 2201 2 0.13054523359820536 2201 3 0.10261798739914241 2201 4 0.09401008381639997 2201 5 0.12387060727417776 2201 6 0.127057655067766 2201 7 0.1122261133504594 2201 8 0.13760575438735145 2201 9 0.1558734400713746 2201 10-11 0.17751737953027913 2201 12-13 0.10912937889538199 2201 14-15 0.22194894063255788 2201 16-17 0.25633629685609804 2201 18-19 0.17631181366645876 2201 20-21 0.21231385323584817 2201 22-23 0.28197599237846305 2201 24-25 0.24112719028643426 2201 26-27 0.2655440719972262 2201 28-29 0.24380469734091292 2201 30-31 0.2120217129747246 2201 32-33 0.23638557741315225 2201 34-35 0.23523187011253555 2201 36-37 0.2455276023604469 2201 38-39 0.21475634862149917 2201 40-41 0.1964212772031999 2201 42-43 0.2621029712045484 2201 44-45 0.3023282459157457 2201 46-47 0.33537226435542067 2201 48-49 0.3688262226964767 2201 50-51 0.30881669653221167 2201 52-53 0.259728349936168 2201 54-55 0.2812137828409149 2201 56-57 0.4283218553054766 2201 58-59 0.3729056625421663 2201 60-61 0.26679285871185954 2201 62-63 0.2874524505095195 2201 64-65 0.278644264498368 2201 66-67 0.15685308123747177 2201 68-69 0.09491286444782077 2201 70-71 0.2830336063408083 2201 72-73 0.12677991219476326 2201 74-75 0.15287207615416776 2201 76-77 0.08081609605699924 2201 78-79 0.3741128945661458 2201 80-81 0.2917973197355579 2201 82-83 0.265029807056429 2201 84-85 0.28577748633803424 2201 86-87 0.2575264906215793 2201 88-89 0.4769453073874885 2201 90-91 0.4970420450672357 2201 92-93 0.32857705138188464 2201 94-95 0.16900237216475134 2201 96-97 0.20523397089424478 2201 98-99 0.21562408413299394 2201 100-101 0.22678546788209175 1101 1 0.12506950309897036 1101 2 0.15077765434761403 1101 3 0.11476357568990636 1101 4 0.11670429700332363 1101 5 0.1516749147311316 1101 6 0.15206234795554252 1101 7 0.1809611301001226 1101 8 0.1702449423686474 1101 9 0.125860295599729 1101 10-11 0.11158812854148437 1101 12-13 0.13546081036650293 1101 14-15 0.2158676269222184 1101 16-17 0.2094844570247929 1101 18-19 0.263485120696231 1101 20-21 0.29386778898976473 1101 22-23 0.2261962734605163 1101 24-25 0.26359521286141785 1101 26-27 0.24723571330621752 1101 28-29 0.27381709621356976 1101 30-31 0.3141344006134901 1101 32-33 0.17574401579553722 1101 34-35 0.21172016245400727 1101 36-37 0.34866239657073805 1101 38-39 0.3824726797832412 1101 40-41 0.40469029896854636 1101 42-43 0.3830312222135177 1101 44-45 0.3767209774658937 1101 46-47 0.4101586612088184 1101 48-49 0.3946465570230586 1101 50-51 0.27659814699418206 1101 52-53 0.32609391887644534 1101 54-55 0.4617740105731585 1101 56-57 0.27487188105057925 1101 58-59 0.4033122310938566 1101 60-61 0.43702347028723665 1101 62-63 0.3640855535258609 1101 64-65 0.3632366038634487 1101 66-67 0.2552637711972352 1101 68-69 0.2928910631332897 1101 70-71 0.4555479876296076 1101 72-73 0.3413208468952007 1101 74-75 0.4131157072037226 1101 76-77 0.1828790917158578 1101 78-79 0.42789890370806916 1101 80-81 0.5248400741361294 1101 82-83 0.5576772580289102 1101 84-85 0.5235982254810239 1101 86-87 0.46978135302347823 1101 88-89 0.5941637099986607 1101 90-91 0.6526761748172483 1101 92-93 0.4585967417741017 1101 94-95 0.3308104343705125 1101 96-97 0.3962540972425721 1101 98-99 0.3737079780544299 1101 100-101 0.5103013596667303 2304 1 0.1338516569169812 2304 2 0.13577696275037 2304 3 0.10620073737483438 2304 4 0.07842303652534355 2304 5 0.14726933528656616 2304 6 0.08515203195862142 2304 7 0.08797353829179855 2304 8 0.07499990364970444 2304 9 0.08335528273165949 2304 10-11 0.0808338961037336 2304 12-13 0.12509641577990038 2304 14-15 0.2428978990867421 2304 16-17 0.2006797818597832 2304 18-19 0.19698220879715933 2304 20-21 0.18386937942103287 2304 22-23 0.18818923815528876 2304 24-25 0.22825319015279177 2304 26-27 0.17768046203908483 2304 28-29 0.22219423016674256 2304 30-31 0.23927337247339864 2304 32-33 0.3393782857637504 2304 34-35 0.3949429445881094 2304 36-37 0.4016260099110056 2304 38-39 0.3481329176118919 2304 40-41 0.34529564934576484 2304 42-43 0.32921016160713634 2304 44-45 0.3385953263666863 2304 46-47 0.32330237988193034 2304 48-49 0.39974189564151175 2304 50-51 0.30605018100913384 2304 52-53 0.3253213457808357 2304 54-55 0.4468478010091772 2304 56-57 0.4452324795278031 2304 58-59 0.31682911737765806 2304 60-61 0.3317705408154552 2304 62-63 0.42268751232961677 2304 64-65 0.3612498069879777 2304 66-67 0.32539443783072386 2304 68-69 0.32814313174426246 2304 70-71 0.4475608979564285 2304 72-73 0.29220693969882916 2304 74-75 0.36130830750106924 2304 76-77 0.40749368582341816 2304 78-79 0.43675028794478976 2304 80-81 0.5642826887744334 2304 82-83 0.4524410630288713 2304 84-85 0.6175593151377186 2304 86-87 0.5287136181837937 2304 88-89 0.5375422360336657 2304 90-91 0.7102826597855945 2304 92-93 0.4710268589432758 2304 94-95 0.5052958226013686 2304 96-97 0.4085845953234717 2304 98-99 0.3956635039890877 2304 100-101 0.39489538279613257 2202 1 0.14723395190615207 2202 2 0.13403146210360006 2202 3 0.07685992034256373 2202 4 0.029469874298740706 2202 5 0.12805326581472087 2202 6 0.13652426178499866 2202 7 0.08428834986625588 2202 8 0.12440287536788475 2202 9 0.14084714589482417 2202 10-11 0.12985033476428498 2202 12-13 0.09738132845750158 2202 14-15 0.17429744575397876 2202 16-17 0.16447954587449232 2202 18-19 0.1413743808684984 2202 20-21 0.16215086068211093 2202 22-23 0.13792826426924876 2202 24-25 0.09746848058154 2202 26-27 0.06625328950439524 2202 28-29 0.10420250926683394 2202 30-31 0.12531858443380628 2202 32-33 0.20273942815995127 2202 34-35 0.22961272542866595 2202 36-37 0.2704994558588041 2202 38-39 0.2523913152153554 2202 40-41 0.28598799586353607 2202 42-43 0.37358894375959295 2202 44-45 0.2494578351415626 2202 46-47 0.19699959221777164 2202 48-49 0.3206804664094136 2202 50-51 0.21712294814948052 2202 52-53 0.23779746376134625 2202 54-55 0.2916325589884323 2202 56-57 0.41862381844862995 2202 58-59 0.45497948513040853 2202 60-61 0.3401124362210126 2202 62-63 0.2098669000113631 2202 64-65 0.26649755231512984 2202 66-67 0.18954761354346772 2202 68-69 0.3008170200849136 2202 70-71 0.2823592856740049 2202 72-73 0.15975428220333043 2202 74-75 0.30446004311286146 2202 76-77 0.06712709219805646 2202 78-79 0.44713595942886997 2202 80-81 0.507820032214525 2202 82-83 0.4481771344044674 2202 84-85 0.37800937333094 2202 86-87 0.4694949717632184 2202 88-89 0.5923740001040301 2202 90-91 0.7267877240468401 2202 92-93 0.5813857246831731 2202 94-95 0.5462461767199471 2202 96-97 0.388674104229505 2202 98-99 0.22138520692326225 2202 100-101 0.29719756802497344 1102 1 0.1004602831498076 1102 2 0.11806290293503707 1102 3 0.08552239353579694 1102 4 0.03636653729743955 1102 5 0.11031493562124695 1102 6 0.08876623237595282 1102 7 0.0425766223241979 1102 8 0.056194547212783164 1102 9 0.038769482893108886 1102 10-11 0.08935445354777016 1102 12-13 0.05363183942176164 1102 14-15 0.15682427686471812 1102 16-17 0.15945742729456214 1102 18-19 0.17594628300533088 1102 20-21 0.16325116564204478 1102 22-23 0.15939397589227156 1102 24-25 0.18166646661417474 1102 26-27 0.13257332178636005 1102 28-29 0.1971609347451988 1102 30-31 0.23268849121746626 1102 32-33 0.0956660349676639 1102 34-35 0.1730975658391003 1102 36-37 0.22053985632859252 1102 38-39 0.18883303620884107 1102 40-41 0.16356981427356487 1102 42-43 0.1638205124212604 1102 44-45 0.1882625000875464 1102 46-47 0.1711311090011307 1102 48-49 0.16995615055969893 1102 50-51 0.008217677769145837 1102 52-53 0.08462711225359953 1102 54-55 0.19055109626440725 1102 56-57 0.16999480587026738 1102 58-59 0.08197645521520514 1102 60-61 0.07885391306274414 1102 62-63 0.1140776553495968 1102 64-65 0.09004468544210198 1102 66-67 0.10873047914903111 1102 68-69 0.02803975921550972 1102 70-71 0.04452701658664182 1102 72-73 0.11326052069977521 1102 74-75 0.11079240437955917 1102 76-77 -0.09246985233166782 1102 78-79 0.25596158625513254 1102 80-81 0.3041747617134689 1102 82-83 0.2076304791148189 1102 84-85 0.21653211460140653 1102 86-87 0.11072526294563545 1102 88-89 0.28345946892505935 1102 90-91 0.35908089017071987 1102 92-93 0.3263686471600806 1102 94-95 0.16725485995748812 1102 96-97 0.017363712025680655 1102 98-99 -0.05468889654364162 1102 100-101 0.05689344072313318 2305 1 0.11269352428588064 2305 2 0.12629235928210392 2305 3 0.08267963302289161 2305 4 0.06090428783646473 2305 5 0.09225925916448574 2305 6 0.00787302792179645 2305 7 0.02157934895033975 2305 8 0.00515159793959441 2305 9 0.008902712385399525 2305 10-11 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-1.7863271278323403 1213 98-99 -1.6772749591187157 1213 100-101 -1.5880799089521211 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 3.0 8 10.0 9 37.0 10 60.0 11 65.0 12 143.0 13 346.0 14 1071.0 15 2691.0 16 4896.0 17 8152.0 18 11818.0 19 15900.0 20 21033.0 21 27118.0 22 34546.0 23 43478.0 24 54749.0 25 68921.0 26 89188.0 27 121424.0 28 180318.0 29 245893.0 30 219810.0 31 214250.0 32 279642.0 33 393358.0 34 593664.0 35 995545.0 36 1815500.0 37 2515258.0 38 574699.0 39 490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.51529100539659 28.332024674086327 16.900039993440465 17.252644327076617 2 30.71181570074889 27.902689417965178 25.088665124535435 16.296829756750494 3 33.21732391212313 21.398670295569165 25.95754643413693 19.426459358170778 4 30.220141378078253 21.070867282240176 24.633491259935063 24.075500079746508 5 34.5186128505754 19.952632253025136 25.082314115515945 20.44644078088351 6 32.32977626051417 20.220979960691707 26.507271963915958 20.941971814878162 7 30.48555807499745 24.181748067189925 22.303700798574937 23.028993059237692 8 28.754556585322582 26.91615039329971 21.879741652202107 22.449551369175598 9 29.63510875282705 22.713059654840237 25.553800369975573 22.098031222357147 10-11 23.979560808019876 29.74593441989412 28.456599966791263 17.81790480529474 12-13 21.48010265199154 29.23435656837875 28.796365441746076 20.48917533788364 14-15 21.63182966359455 29.311465082867194 28.89911820575324 20.157587047785018 16-17 23.840581735279724 26.887791364326457 28.901377288061298 20.370249612332522 18-19 21.424021601867434 26.727934374156693 31.16018287625012 20.68786114772576 20-21 23.50740449142889 27.172639759402216 30.670159174800038 18.649796574368853 22-23 21.366311787437585 31.404022954093335 28.220198856888683 19.009466401580394 24-25 21.354746389093982 26.709953519285097 30.59409353817642 21.341206553444504 26-27 21.313968692806498 26.973121990814004 28.66664432486372 23.046264991515777 28-29 23.018546703996225 29.559283329644202 28.523828646026985 18.89834132033259 30-31 20.6515947770333 27.968935597839113 30.627576948274548 20.751892676853046 32-33 20.359372196899557 30.209248170598883 28.60896380475592 20.822415827745637 34-35 22.586935880705084 30.255884731295485 28.703789525217548 18.45338986278188 36-37 22.43908743734091 29.919814409923884 29.063723001327503 18.577375151407704 38-39 20.647982946096512 27.509010553462073 32.918190103870664 18.92481639657075 40-41 20.598307469846535 27.5033776952643 28.284581965864053 23.61373286902511 42-43 20.682993873576933 29.440502497464806 30.405400654850666 19.471102974107595 44-45 20.531163509194762 27.233400986540428 30.663958194805968 21.571477309458846 46-47 22.67488744793971 27.415424312346566 28.429563242870405 21.480124996843323 48-49 20.49453604038357 29.95041116219942 30.25768125425682 19.297371543160192 50-51 22.842158754399 27.92710194172681 29.667542438489292 19.5631968653849 52-53 20.569121067994512 27.823899777899573 27.604553193538848 24.002425960567066 54-55 22.81584643173074 27.792221734159217 29.7376144376225 19.65431739648754 56-57 20.755134039022703 27.477776972999045 29.9495122454897 21.81757674248854 58-59 20.77719917574899 27.73159145589119 30.001182625092493 21.49002674326733 60-61 22.51874438658789 27.906600323651336 29.848900981373582 19.72575430838719 62-63 20.229347213519304 27.944225971465166 29.82492890432087 22.001497910694663 64-65 22.317800147852505 27.686161024223154 29.93950540196052 20.056533425963817 66-67 20.660893609113828 31.33364872602624 27.899900149087802 20.105557515772123 68-69 20.52221778204332 31.71406474624729 27.830568633376814 19.933148838332578 70-71 20.366140541506088 32.086457706326996 27.749782768035995 19.797618984130917 72-73 20.33011128470626 31.98581344582815 27.60444144955211 20.079633819913482 74-75 20.61185387321065 31.421459449920864 27.597457721733328 20.369228955135167 76-77 20.767790489348084 30.88888142654797 27.760423928164148 20.582904155939797 78-79 20.676525710283492 30.526576159142977 28.026746978547546 20.77015115202599 80-81 20.390365429038575 30.673482933707337 27.865305991389977 21.070845645864114 82-83 20.23317767804637 30.507457796512423 27.855494100807 21.403870424634206 84-85 20.432098363961916 30.290900497987305 28.005517814862817 21.271483323187965 86-87 20.42555493227685 30.071031894028348 28.54378874117991 20.959624432514897 88-89 20.710570781148608 30.38445357431118 28.147540506140917 20.75743513839929 90-91 20.826114264263225 29.928851027362942 27.8169722652967 21.42806244307713 92-93 20.60043404107764 30.186891106049814 27.660594085079598 21.552080767792948 94-95 20.249671981681967 30.16803740273381 27.61128517826114 21.971005437323075 96-97 20.18499685981114 30.516030669998383 27.688067305769533 21.610905164420945 98-99 20.496567241231368 30.225356557244453 27.61096682260454 21.667109378919644 100-101 20.684694227245448 30.220326496847328 27.206824142458863 21.888155133448365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22.0 1 18.5 2 21.5 3 37.0 4 48.5 5 43.0 6 39.5 7 34.5 8 40.0 9 50.5 10 58.0 11 66.0 12 78.0 13 76.5 14 95.0 15 142.0 16 178.0 17 245.5 18 371.5 19 589.0 20 902.5 21 1432.0 22 2272.5 23 3672.5 24 5587.0 25 8060.0 26 11514.5 27 16548.0 28 22645.0 29 29218.5 30 36745.5 31 45883.5 32 62767.0 33 91737.5 34 134232.5 35 196960.0 36 273958.0 37 364169.0 38 457277.5 39 514068.0 40 560332.5 41 573385.5 42 542956.0 43 536487.0 44 525856.0 45 513189.5 46 525365.0 47 497349.0 48 412567.5 49 330009.5 50 267514.0 51 218333.5 52 176202.0 53 140019.0 54 113568.0 55 90934.5 56 67416.5 57 47158.5 58 33219.0 59 24100.5 60 17457.5 61 12253.5 62 8259.5 63 5454.5 64 3682.5 65 2566.0 66 1785.0 67 1239.5 68 897.0 69 658.5 70 465.5 71 350.5 72 272.0 73 191.5 74 137.5 75 116.5 76 89.5 77 75.5 78 55.5 79 36.5 80 34.5 81 32.5 82 27.5 83 18.5 84 14.0 85 13.5 86 14.0 87 7.5 88 2.5 89 2.5 90 2.0 91 0.5 92 0.5 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17787512346092027 2 0.0 3 0.0 4 0.009233570308775044 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0020857557296471542 12-13 0.0 14-15 0.0 16-17 2.7536662722869735E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002284957119557276 40-41 0.006110795578713433 42-43 0.0026130535264680646 44-45 0.0013299622208705173 46-47 0.00681385930780798 48-49 0.008260998816860921 50-51 0.005407731849618886 52-53 0.006444750850033342 54-55 0.017353956379817057 56-57 0.020049034008012816 58-59 0.025896180688315795 60-61 0.02409165045030646 62-63 0.010053811326052015 64-65 0.01463544329398481 66-67 0.021753963551067092 68-69 0.02505836307781146 70-71 0.02693319968873025 72-73 0.020769674330334727 74-75 0.03041336514774826 76-77 0.027519086129642375 78-79 0.019615478041737845 80-81 0.008964062545955467 82-83 0.0018221068312366997 84-85 0.00734115710462889 86-87 0.02501735102694761 88-89 0.02351162287380346 90-91 0.019896703533375665 92-93 0.04157450184712418 94-95 0.04317397183081428 96-97 0.03214173014843902 98-99 0.03900831923592909 100-101 0.03873295260870039 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8534077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.37893088575072 #Duplication Level Percentage of deduplicated Percentage of total 1 88.61090747586277 40.210682460708284 2 5.370952630698302 4.874561764381985 3 1.7176454841963844 2.3383474714070864 4 0.9881653499195318 1.7936754847076846 5 0.47653375722536484 1.0812296216928472 6 0.40141623299330376 1.0929503696052925 7 0.2845346813413992 0.903831574743332 8 0.1906680905851072 0.6921851283823711 9 0.1859819353534801 0.7595695251363358 >10 1.41128868334291 13.013351354614839 >50 0.21299104277971898 6.815160636127376 >100 0.1253708204760777 10.42910780648323 >500 0.013050910831881068 4.143246267343592 >1k 0.010415419838244289 8.123473384978867 >5k 5.171472341807687E-5 0.12742526287370476 >10k+ 2.57698321905903E-5 3.6012018868130906 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 307951 3.6084863073065785 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002812254916378186 0.0 1.1717728818242441E-5 2 0.0030466094927430348 0.0 1.1717728818242441E-5 3 0.0033043995267443686 0.0 2.3435457636484882E-5 4 0.00350360091665449 0.0 2.3435457636484882E-5 5 0.0040777696287483695 0.0 2.3435457636484882E-5 6 0.004394148306840915 0.0 3.515318645472732E-5 7 0.0051675184088449165 0.0 3.515318645472732E-5 8 0.005671380748029342 0.0 3.515318645472732E-5 9 0.006327573561850919 0.0 3.515318645472732E-5 10-11 0.009831174478505409 0.0 3.515318645472732E-5 12-13 0.012924654886521413 0.0 3.515318645472732E-5 14-15 0.017213343633998145 0.0 3.515318645472732E-5 16-17 0.02149617351706576 0.0 4.101205086384854E-5 18-19 0.027460497485551162 0.0 5.272977968209099E-5 20-21 0.03409273199667638 0.0 5.8588644091212205E-5 22-23 0.0421603882880363 0.0 5.8588644091212205E-5 24-25 0.051376382003583985 0.0 6.444750850033342E-5 26-27 0.06175828973654679 0.0 7.030637290945465E-5 28-29 0.07361077243619901 0.0 7.030637290945465E-5 30-31 0.10932055100979285 0.0 7.030637290945465E-5 32-33 0.12507503740591983 0.0 7.030637290945465E-5 34-35 0.13979836366604143 0.0 7.030637290945465E-5 36-37 0.1545802785702543 0.0 7.616523731857587E-5 38-39 0.1715358321702511 0.0 8.20241017276971E-5 40-41 0.1898623600419823 0.0 9.960069495506075E-5 42-43 0.20940167284640154 0.0 1.1717728818242441E-4 44-45 0.2288003729050019 0.0 1.1717728818242441E-4 46-47 0.24870879416719582 0.0 1.1717728818242441E-4 48-49 0.2701346613113521 0.0 1.1717728818242441E-4 50-51 0.2923280396931033 0.0 1.2303615259154563E-4 52-53 0.3151893286174943 0.0 1.2889501700066684E-4 54-55 0.33928097906780075 0.0 1.2889501700066684E-4 56-57 0.36354253658597174 0.0 1.2889501700066684E-4 58-59 0.38902859676564905 0.0 1.2889501700066684E-4 60-61 0.41621958648838064 0.0 1.2889501700066684E-4 62-63 0.44430112360129864 0.0 1.464716102280305E-4 64-65 0.4734958449519497 0.0 1.5233047463715174E-4 66-67 0.5055965630495248 0.0 1.5233047463715174E-4 68-69 0.538371050554149 0.0 1.5233047463715174E-4 70-71 0.5747311630771552 0.0 1.5233047463715174E-4 72-73 0.6126555923973969 0.0 1.5233047463715174E-4 74-75 0.6542828240242032 0.0 1.5818933904627298E-4 76-77 0.6989859594657981 0.0 1.640482034553942E-4 78-79 0.7465657973322715 0.0 1.640482034553942E-4 80-81 0.7991080933532706 0.0 1.640482034553942E-4 82-83 0.8550719661891966 5.8588644091212205E-6 1.640482034553942E-4 84-85 0.9175040253327923 1.1717728818242441E-5 1.640482034553942E-4 86-87 0.9860703155127379 1.1717728818242441E-5 1.640482034553942E-4 88-89 1.0607766955934426 1.1717728818242441E-5 1.699070678645154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 47150 0.0 71.932236 3 CGGAAGA 47190 0.0 71.730354 4 GATCGGA 48450 0.0 69.983025 1 GAAGAGC 48550 0.0 69.72508 6 ATCGGAA 50015 0.0 67.631325 2 GGAAGAG 55220 0.0 61.509434 5 AGAGCAC 76405 0.0 44.37997 8 GAGCACA 76735 0.0 44.17055 9 AAGAGCA 76740 0.0 44.09341 7 GCCGTCT 38480 0.0 44.038746 50-51 ATGCGCG 65 2.5633271E-6 43.91548 1 CGTCTTC 38620 0.0 43.831833 52-53 ATGCCGT 38515 0.0 43.806408 48-49 TGCCGTC 38550 0.0 43.515186 50-51 CCGTCTT 38525 0.0 43.508347 52-53 TCTCGTA 38445 0.0 43.449276 42-43 TCGTATG 39090 0.0 43.341255 44-45 TATGCCG 38530 0.0 43.320885 48-49 CGTATGC 38820 0.0 43.087135 46-47 CTGCTTG 39550 0.0 42.99034 58-59 >>END_MODULE