##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2676500 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66132897440688 33.0 33.0 33.0 33.0 33.0 2 32.66363796002242 33.0 33.0 33.0 33.0 33.0 3 32.72584345226976 33.0 33.0 33.0 33.0 33.0 4 36.66017859144405 37.0 37.0 37.0 37.0 37.0 5 36.62153558752102 37.0 37.0 37.0 37.0 37.0 6 36.54050588455072 37.0 37.0 37.0 37.0 37.0 7 36.43415580048571 37.0 37.0 37.0 37.0 37.0 8 36.37623089856155 37.0 37.0 37.0 37.0 37.0 9 36.41044759947693 37.0 37.0 37.0 37.0 37.0 10-11 36.34297347281898 37.0 37.0 37.0 37.0 37.0 12-13 36.33417522884364 37.0 37.0 37.0 37.0 37.0 14-15 38.14203848309359 40.0 37.0 40.0 35.0 40.0 16-17 38.15380814496544 40.0 37.0 40.0 37.0 40.0 18-19 38.15945768727816 40.0 37.0 40.0 37.0 40.0 20-21 38.1436224546983 40.0 37.0 40.0 37.0 40.0 22-23 37.90934933682047 40.0 37.0 40.0 33.0 40.0 24-25 37.997479917803105 40.0 37.0 40.0 35.0 40.0 26-27 37.92250569773958 40.0 37.0 40.0 33.0 40.0 28-29 37.69920679992528 40.0 37.0 40.0 33.0 40.0 30-31 37.69872333271063 40.0 37.0 40.0 33.0 40.0 32-33 37.579175415654774 40.0 37.0 40.0 33.0 40.0 34-35 37.5681926022791 40.0 37.0 40.0 33.0 40.0 36-37 37.46241247898375 40.0 37.0 40.0 33.0 40.0 38-39 37.13417616289931 38.5 37.0 40.0 33.0 40.0 40-41 36.97373136558939 37.0 37.0 40.0 33.0 40.0 42-43 36.755652904913134 37.0 37.0 40.0 33.0 40.0 44-45 36.56752624696432 37.0 37.0 40.0 33.0 40.0 46-47 36.68953577433215 37.0 37.0 40.0 33.0 40.0 48-49 36.24571567345414 37.0 37.0 40.0 33.0 40.0 50-51 36.217926396413226 37.0 37.0 40.0 33.0 40.0 52-53 36.23996207733981 37.0 37.0 40.0 33.0 40.0 54-55 35.70444218195405 37.0 35.0 40.0 30.0 40.0 56-57 35.56877620026154 37.0 33.0 40.0 27.0 40.0 58-59 35.466324490939655 37.0 33.0 40.0 27.0 40.0 60-61 35.10384158415842 37.0 33.0 38.5 27.0 40.0 62-63 34.84969624509621 37.0 33.0 37.0 27.0 40.0 64-65 34.59862637773212 37.0 33.0 37.0 27.0 40.0 66-67 33.782090229777694 37.0 33.0 37.0 27.0 40.0 68-69 33.72580029889781 37.0 33.0 37.0 27.0 37.0 70-71 33.32035979824397 37.0 33.0 37.0 27.0 37.0 72-73 32.543195591257245 37.0 33.0 37.0 22.0 37.0 74-75 31.53822940407248 33.0 33.0 37.0 18.5 37.0 76-77 29.0179796375864 33.0 27.0 33.0 15.0 37.0 78-79 31.039257425742576 33.0 33.0 37.0 15.0 37.0 80-81 31.411841584158417 33.0 33.0 37.0 15.0 37.0 82-83 31.259929011769103 33.0 33.0 37.0 15.0 37.0 84-85 31.139873715673453 33.0 33.0 37.0 15.0 37.0 86-87 31.020978516719595 33.0 33.0 37.0 15.0 37.0 88-89 30.86349318139361 33.0 33.0 37.0 15.0 37.0 90-91 30.643422753596113 33.0 33.0 37.0 15.0 37.0 92-93 30.58600560433402 33.0 33.0 37.0 15.0 37.0 94-95 30.461158976274987 33.0 30.0 37.0 15.0 37.0 96-97 30.289798617597608 33.0 27.0 37.0 15.0 37.0 98-99 30.122336633663366 33.0 27.0 37.0 15.0 37.0 100-101 28.570241920418457 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1333273875115708 2306 2 0.1584604481256946 2306 3 0.11251995647955226 2306 4 0.12212366463426605 2306 5 0.19218484539764802 2306 6 0.13988918287701324 2306 7 0.15753368343524699 2306 8 0.19498281594091793 2306 9 0.23726555596653043 2306 10-11 0.15927186360973167 2306 12-13 0.14332650270137037 2306 14-15 0.3762367355452696 2306 16-17 0.33301173690746566 2306 18-19 0.35091776708510736 2306 20-21 0.38439332576335516 2306 22-23 0.4350590515231687 2306 24-25 0.426177547994655 2306 26-27 0.35423222925995645 2306 28-29 0.4667778153760693 2306 30-31 0.38654439553321396 2306 32-33 0.2738691978011474 2306 34-35 0.3785063950204517 2306 36-37 0.49463148441602556 2306 38-39 0.4776455467885299 2306 40-41 0.4937720535702468 2306 42-43 0.527648754462426 2306 44-45 0.43007308341515227 2306 46-47 0.21644504782714336 2306 48-49 0.21903253992424965 2306 50-51 0.015351426878581265 2306 52-53 -0.035016535984240704 2306 54-55 0.01615859322171076 2306 56-57 0.24283861937331608 2306 58-59 0.32511707716148663 2306 60-61 0.2040959269047775 2306 62-63 0.02493113150887183 2306 64-65 0.0040029165764181585 2306 66-67 0.014402007004655104 2306 68-69 0.2851613119856182 2306 70-71 0.4904663454393372 2306 72-73 0.3070811484248068 2306 74-75 0.5287280215695098 2306 76-77 0.20957824137035175 2306 78-79 0.6407588735381466 2306 80-81 0.8075464696600392 2306 82-83 0.9338610548214596 2306 84-85 0.825768523417981 2306 86-87 0.7395665127204616 2306 88-89 0.9239003604272717 2306 90-91 1.064121236084631 2306 92-93 0.9293067450236805 2306 94-95 0.8757082559479734 2306 96-97 0.9104961556081044 2306 98-99 0.7426277206922229 2306 100-101 0.7461638854003816 2307 1 0.14135067600824414 2307 2 0.16546172449779561 2307 3 0.11911045472670168 2307 4 0.15705063420347187 2307 5 0.19620719827314304 2307 6 0.21229514428552676 2307 7 0.14952964640313127 2307 8 0.15075811092147973 2307 9 0.20967122860307796 2307 10-11 0.16148674460680468 2307 12-13 0.25133720555308514 2307 14-15 0.2862829438730685 2307 16-17 0.28980543436299655 2307 18-19 0.3374889079846497 2307 20-21 0.3284098261936279 2307 22-23 0.2921480905093077 2307 24-25 0.36073857128700126 2307 26-27 0.3318776692618073 2307 28-29 0.37639988987539397 2307 30-31 0.45626489795794356 2307 32-33 0.4618767145354852 2307 34-35 0.4951954174503328 2307 36-37 0.2313106741603903 2307 38-39 0.28386547840732845 2307 40-41 0.47226082804126435 2307 42-43 0.28878741121073404 2307 44-45 0.40453757986421834 2307 46-47 0.3548794417236607 2307 48-49 0.43708497513826217 2307 50-51 0.24148285951481796 2307 52-53 0.17525626208136913 2307 54-55 0.3487869619873649 2307 56-57 0.24551986709923312 2307 58-59 0.3712905116805203 2307 60-61 0.1267708553374547 2307 62-63 0.20841274924794817 2307 64-65 -0.03262299473978203 2307 66-67 0.09925319799651078 2307 68-69 0.2896985212163088 2307 70-71 0.48059599924025065 2307 72-73 0.2756519293219597 2307 74-75 0.32971817252024493 2307 76-77 0.28215012633900116 2307 78-79 0.5986831132496846 2307 80-81 0.5813786998844606 2307 82-83 0.5952685823366117 2307 84-85 0.5877590574728551 2307 86-87 0.5799065369002996 2307 88-89 0.7359259321476408 2307 90-91 0.8535689308180068 2307 92-93 0.627803542542555 2307 94-95 0.4313048010801488 2307 96-97 0.28354032713095023 2307 98-99 0.40677701772499475 2307 100-101 0.5803092695729823 2201 1 0.10291790001824097 2201 2 0.14249757582867062 2201 3 0.10707476927181858 2201 4 0.0613851120811475 2201 5 0.15790919652591384 2201 6 0.16179350764241462 2201 7 0.17064842467116392 2201 8 0.19790352334448613 2201 9 0.213564476344402 2201 10-11 0.2722487097949653 2201 12-13 0.2112795360001769 2201 14-15 0.3007995121199798 2201 16-17 0.34419928202248684 2201 18-19 0.25980503444850456 2201 20-21 0.3677086130872098 2201 22-23 0.31955184096546674 2201 24-25 0.28977129309085115 2201 26-27 0.24272554439036043 2201 28-29 0.3308835179150691 2201 30-31 0.3395835789120625 2201 32-33 0.23423368796215271 2201 34-35 0.26014222654918484 2201 36-37 0.3166203508127907 2201 38-39 0.21771828005281435 2201 40-41 0.4249108352292268 2201 42-43 0.38435755463817856 2201 44-45 0.32958132965686815 2201 46-47 0.3497815195969025 2201 48-49 0.40267814768246524 2201 50-51 0.34526386450215796 2201 52-53 0.2891702166755792 2201 54-55 0.3430600933943211 2201 56-57 0.36489045065096803 2201 58-59 0.4319886837270914 2201 60-61 0.21695763170344406 2201 62-63 0.29577721474021246 2201 64-65 0.1994088340983069 2201 66-67 0.2118204468879057 2201 68-69 0.3030425535510659 2201 70-71 0.5157091819581794 2201 72-73 0.2155574767680264 2201 74-75 0.17071186646798608 2201 76-77 0.11751613171338349 2201 78-79 0.444197619105104 2201 80-81 0.44462258458958814 2201 82-83 0.19752698395555868 2201 84-85 0.18184080945507475 2201 86-87 0.1877166005966835 2201 88-89 0.2775756656402173 2201 90-91 0.49838178123103916 2201 92-93 0.2605098646294941 2201 94-95 0.26710070173148637 2201 96-97 0.22511361943762154 2201 98-99 0.13681729794483388 2201 100-101 0.3237568223491927 1101 1 0.09519430303264187 1101 2 0.143347805795635 1101 3 0.10640808101200605 1101 4 0.11129361114184633 1101 5 0.14709643066035483 1101 6 0.14083601374247934 1101 7 0.20206104802198865 1101 8 0.1717123916479153 1101 9 0.19215082731933109 1101 10-11 0.1717211623440278 1101 12-13 0.15818469892043652 1101 14-15 0.28405605484201146 1101 16-17 0.2606263997698903 1101 18-19 0.24866567230991876 1101 20-21 0.29987915572286994 1101 22-23 0.25207799565874467 1101 24-25 0.1972716709708351 1101 26-27 0.28645840483387985 1101 28-29 0.3247456672964262 1101 30-31 0.3354026067888043 1101 32-33 0.20587851856395645 1101 34-35 0.2609816599451378 1101 36-37 0.30365029510248576 1101 38-39 0.3551451332842248 1101 40-41 0.3137156008356712 1101 42-43 0.49156345604389173 1101 44-45 0.5129428690319671 1101 46-47 0.5437365194889381 1101 48-49 0.5730669322044974 1101 50-51 0.3407090611077166 1101 52-53 0.4551108139418929 1101 54-55 0.4423318646797654 1101 56-57 0.20273810023746108 1101 58-59 0.41006354715047166 1101 60-61 0.46540832376364705 1101 62-63 0.40618632380716946 1101 64-65 0.45471602353494234 1101 66-67 0.12663482684083505 1101 68-69 0.5229241691499524 1101 70-71 0.6283336124390573 1101 72-73 0.34091848711114636 1101 74-75 0.4912856772256866 1101 76-77 0.11715039787045001 1101 78-79 0.4253771714792691 1101 80-81 0.43719251937829995 1101 82-83 0.5069162219335759 1101 84-85 0.4259263128991826 1101 86-87 0.46693819481835774 1101 88-89 0.4855007536322695 1101 90-91 0.5639041463278822 1101 92-93 0.4973164548564384 1101 94-95 0.42830835218170193 1101 96-97 0.42287238625328527 1101 98-99 0.35488239047144887 1101 100-101 0.3972834952778719 2304 1 0.11504301524753657 2304 2 0.13738442968347897 2304 3 0.09708098591273284 2304 4 0.07834739724617634 2304 5 0.1153694936780667 2304 6 0.07753813492280415 2304 7 0.1523815060583118 2304 8 0.07667677013692753 2304 9 0.13269093987945269 2304 10-11 0.13147490612927726 2304 12-13 0.04067005914937738 2304 14-15 0.21712735955475893 2304 16-17 0.22599135178665364 2304 18-19 0.2376568159508068 2304 20-21 0.26031081039912607 2304 22-23 0.3139809556764348 2304 24-25 0.40976230438577943 2304 26-27 0.15677535789112795 2304 28-29 0.30486876200433954 2304 30-31 0.3238914814272533 2304 32-33 0.2796372078275198 2304 34-35 0.2515779425454667 2304 36-37 0.2908982814094898 2304 38-39 0.29156139434387285 2304 40-41 0.2953117101517293 2304 42-43 0.3138776184776688 2304 44-45 0.4387023433794113 2304 46-47 0.14369905314872256 2304 48-49 0.21234436819442948 2304 50-51 0.2305391599976332 2304 52-53 0.36599288986398193 2304 54-55 0.4552766129283512 2304 56-57 0.4448261600861585 2304 58-59 0.30765381509669254 2304 60-61 0.19943129709398022 2304 62-63 0.21900590265171616 2304 64-65 0.24925401267501002 2304 66-67 0.24112426101854822 2304 68-69 0.17517719877606197 2304 70-71 0.3353749267905002 2304 72-73 0.21516370833543874 2304 74-75 0.13015269273993724 2304 76-77 0.18701559370330045 2304 78-79 0.25204200706942714 2304 80-81 0.40078798742463917 2304 82-83 0.31607168307997924 2304 84-85 0.4195132228239693 2304 86-87 0.4021100311326897 2304 88-89 0.5050328585726014 2304 90-91 0.5450099711551601 2304 92-93 0.2774555420137155 2304 94-95 0.33049301113977236 2304 96-97 0.21531554134742947 2304 98-99 0.27261666119021655 2304 100-101 0.303399271180826 2202 1 0.14463893657413962 2202 2 0.14746819192409077 2202 3 0.06025142066658873 2202 4 0.025770418195705247 2202 5 0.0813902226427956 2202 6 0.05009721542721479 2202 7 0.03969190125103239 2202 8 0.10190200582046316 2202 9 0.12384086869685262 2202 10-11 0.10178400431060908 2202 12-13 0.12994459815100612 2202 14-15 0.11885913534872827 2202 16-17 0.2157866653084568 2202 18-19 0.11542518948726865 2202 20-21 0.13695313743525617 2202 22-23 0.14042178335755295 2202 24-25 0.05447291113874542 2202 26-27 0.042377335455327625 2202 28-29 0.11220540699083159 2202 30-31 0.20840232661644364 2202 32-33 0.15692997534446818 2202 34-35 0.24516755083536879 2202 36-37 0.15325272820265212 2202 38-39 0.1484607069313526 2202 40-41 0.09363268370880462 2202 42-43 0.27363508755008326 2202 44-45 0.2315143604519747 2202 46-47 0.31875926902178975 2202 48-49 0.32760178819359 2202 50-51 0.522411837592685 2202 52-53 0.2888198329449878 2202 54-55 0.3417962955246523 2202 56-57 0.4351109749216846 2202 58-59 0.3082307336410679 2202 60-61 0.41372346904807245 2202 62-63 0.2933335902742442 2202 64-65 0.27461266077193613 2202 66-67 0.08457134580341119 2202 68-69 0.09351429087443108 2202 70-71 0.25342883631067536 2202 72-73 -0.20705061057176266 2202 74-75 -0.04709330567916581 2202 76-77 -0.08834155933032761 2202 78-79 0.18860779038233844 2202 80-81 0.3776165289921458 2202 82-83 0.23926131092882486 2202 84-85 0.1870730051286813 2202 86-87 0.1692978944550596 2202 88-89 0.24865148867951703 2202 90-91 0.30977565604014146 2202 92-93 0.30340649901252803 2202 94-95 0.11137204855821992 2202 96-97 -0.1376066390247459 2202 98-99 -0.14073615369737524 2202 100-101 -4.200175278583629E-4 1102 1 0.13613699342042196 1102 2 0.1625702462332228 1102 3 0.10858164454075592 1102 4 0.028410875875032104 1102 5 0.09976805602123306 1102 6 0.0982297837578372 1102 7 0.06332069174373345 1102 8 0.055514246965742586 1102 9 0.03873362851231121 1102 10-11 0.09567355549478407 1102 12-13 0.08254807289414146 1102 14-15 0.18511833545671408 1102 16-17 0.11016818596139188 1102 18-19 0.1835003068581571 1102 20-21 0.14702082929370164 1102 22-23 0.14437678814054067 1102 24-25 0.21522712888077677 1102 26-27 0.21936737210080537 1102 28-29 0.2172071597203029 1102 30-31 0.2447339292585511 1102 32-33 0.206677017570442 1102 34-35 0.3151355471660011 1102 36-37 0.32850415362969443 1102 38-39 0.1074867816231162 1102 40-41 0.10020731313909437 1102 42-43 0.2111435671106392 1102 44-45 0.09974732677959963 1102 46-47 0.2363122865057008 1102 48-49 0.2354872457730579 1102 50-51 0.21077584885698286 1102 52-53 0.29042500945414673 1102 54-55 0.43189829818164327 1102 56-57 0.3487961075747279 1102 58-59 0.43265903047699084 1102 60-61 0.26103834948023774 1102 62-63 0.2997697919545246 1102 64-65 0.3927306149888494 1102 66-67 0.3172405014147941 1102 68-69 0.2999529374229226 1102 70-71 0.21674737646953446 1102 72-73 0.02550714857910208 1102 74-75 0.141003663992759 1102 76-77 -0.23802896054559497 1102 78-79 0.2074594461090804 1102 80-81 0.30908670056680165 1102 82-83 0.19445944511558722 1102 84-85 0.19685075627690551 1102 86-87 0.17312652365532344 1102 88-89 0.18724483546995785 1102 90-91 0.32744516632959275 1102 92-93 0.14999015723785902 1102 94-95 0.18620429960692775 1102 96-97 0.12629346985797696 1102 98-99 -0.010498023532591105 1102 100-101 0.2977592099075572 2305 1 0.0959357240057912 2305 2 0.0880391479131859 2305 3 0.049766721660148505 2305 4 -0.0024420768309383334 2305 5 0.11661136226727109 2305 6 0.009664193708680102 2305 7 -0.037445224574156555 2305 8 0.0673514750521278 2305 9 0.012654702399544249 2305 10-11 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sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 6.0 10 14.0 11 13.0 12 26.0 13 97.0 14 264.0 15 756.0 16 1641.0 17 2647.0 18 3631.0 19 4734.0 20 6332.0 21 8942.0 22 11961.0 23 16530.0 24 21547.0 25 28420.0 26 38885.0 27 57995.0 28 90098.0 29 103080.0 30 67823.0 31 60126.0 32 79842.0 33 114885.0 34 179413.0 35 320104.0 36 599935.0 37 740850.0 38 115881.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44319681945457 21.66256260426246 21.435220378606775 12.4590201976762 2 31.47588268260788 27.036764431160098 29.74616103119746 11.741191855034561 3 38.24722585466094 14.918064636652344 31.974182701288996 14.86052680739772 4 31.45585148240344 14.247633452928001 30.044181025624546 24.25233403904401 5 38.826004109844945 13.128413973472819 32.52579861759761 15.519783299084624 6 33.79476928824958 13.780459555389502 36.73457874089296 15.690192415467962 7 33.78882869419017 23.496543994022044 25.36443116009714 17.350196151690643 8 30.039006164767418 33.32669531104054 19.97937605081263 16.654922473379415 9 38.286530917242665 23.730282084812256 20.433401830749112 17.549785167195964 10-11 30.159207872552997 28.812324440170105 25.206262950981866 15.822204736295031 12-13 25.40003736222679 27.216289930879878 24.842592938539138 22.541079768354194 14-15 24.219390995703343 28.007696618718477 24.69045395105548 23.0824584345227 16-17 27.852539817044104 24.934485139503497 25.459556763106335 21.753418280346065 18-19 22.203194470390436 25.03713805342798 29.928899682421072 22.830767793760508 20-21 26.09291985802354 25.686269381655148 30.236334765552026 17.98447599476929 22-23 22.063198206613112 33.54675882682608 25.767775079394735 18.622267887166075 24-25 22.73801606575752 23.051429105174666 31.075976088174855 23.13457874089296 26-27 20.652400523071172 23.1132449093966 27.52841397347282 28.705940594059403 28-29 24.579656267513545 29.373248645619277 25.41770969549785 20.629385391369325 30-31 18.55010274612367 26.010891089108913 30.430935923781057 25.008070240986363 32-33 19.131029329348028 28.76816738277601 26.67818045955539 25.422622828320566 34-35 20.04929945824771 24.141378666168503 30.6824397534093 25.126882122174482 36-37 23.565010274612366 26.62897440687465 26.416196525312905 23.389818793200075 38-39 23.638427438872544 30.821187052261468 24.27619710013669 21.264188408729293 40-41 24.68114704115215 29.914486801239914 24.089866951237372 21.314499206370556 42-43 21.21350647914352 30.263496129551488 29.02066705175618 19.502330339548816 44-45 19.132702862302114 26.950366799186014 28.865785441951502 25.051144896560366 46-47 24.189519615411346 26.3547737672037 25.411965860508147 24.043740756876808 48-49 19.98349206681341 29.757249684170876 30.402770403345826 19.856487845669882 50-51 23.889959785036954 25.435356959639694 28.531980105867394 22.14270314945596 52-53 19.04941433540141 26.33552396697179 24.787763489285748 29.82729820834105 54-55 24.243842001426376 26.284565667972654 29.845817179784166 19.625775150816803 56-57 20.243113024611382 25.61281814319139 28.50626897218453 25.6377998600127 58-59 19.02812946538936 24.87595712441398 29.511020770139346 26.584892640057312 60-61 22.745820770356506 24.869237910018942 31.232781185207553 21.152160134417 62-63 18.893523113162423 24.870171555146356 30.973248128706715 25.263057202984502 64-65 24.532720102522347 26.501948077790082 29.300461361769248 19.664870457918322 66-67 20.28326080656345 34.694403788470346 24.649441488750455 20.372893916215745 68-69 19.695247477353124 32.86826552179237 26.699125367809117 20.73736163304539 70-71 19.33122179555456 35.715054516915664 25.379151834515234 19.574571853014547 72-73 21.235407898700043 35.08014397963478 24.63463224429302 19.04981587737216 74-75 22.543192952189884 32.879434219791094 24.905051747825215 19.672321080193804 76-77 21.718563282665343 31.212883104846206 25.25087802403249 21.817675588455955 78-79 21.759672402169837 32.12036858492864 25.226516500221972 20.893442512679542 80-81 21.227384484317668 32.28088825450442 25.21696322479879 21.274764036379125 82-83 20.57522260284529 31.342282593852065 26.061500276764882 22.020994526537763 84-85 21.18833393739888 30.599930501324586 25.983294162484917 22.22844139879162 86-87 19.364245558265843 31.245793334586537 27.24342097149539 22.14654013565223 88-89 19.12597477970642 31.510642742653673 27.2461648792669 22.11721759837301 90-91 20.52509221962003 30.326213242479582 26.016060096810516 23.132634441089873 92-93 20.682229768497148 30.662774333777516 25.106500283141052 23.548495614584287 94-95 20.980890579330534 31.712981349592674 25.007293565010475 22.29883450606632 96-97 20.43361449500313 32.88451616266925 23.911305973126655 22.770563369200957 98-99 20.63636677945259 30.565303237152264 25.59287364892835 23.2054563344668 100-101 21.79939538736174 31.36850422660714 24.946494622176903 21.88560576385421 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 154.5 2 106.0 3 218.0 4 259.0 5 188.0 6 173.0 7 184.5 8 183.5 9 196.0 10 205.5 11 211.5 12 201.5 13 197.0 14 210.5 15 211.5 16 211.0 17 234.0 18 269.5 19 283.5 20 304.5 21 348.0 22 380.5 23 432.5 24 517.5 25 650.0 26 828.5 27 1077.0 28 1397.0 29 1730.5 30 2335.0 31 3469.5 32 5794.0 33 10025.5 34 16808.0 35 28602.5 36 46107.5 37 69542.5 38 96745.0 39 117890.5 40 133188.0 41 142298.0 42 175278.5 43 217285.5 44 210900.0 45 185508.0 46 178855.0 47 167186.0 48 138227.0 49 114515.0 50 97330.0 51 88171.5 52 85494.5 53 85661.5 54 71599.0 55 43808.0 56 28331.0 57 22696.5 58 19614.0 59 15807.0 60 12036.0 61 9194.0 62 6695.5 63 4700.0 64 3463.5 65 2530.0 66 1848.0 67 1390.0 68 1076.5 69 793.5 70 599.0 71 532.0 72 347.5 73 182.0 74 114.5 75 79.5 76 51.5 77 32.0 78 29.0 79 20.0 80 11.5 81 7.5 82 4.5 83 3.0 84 2.0 85 1.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1777321128339249 2 0.0 3 0.0 4 0.008892209975714552 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0023164580609004296 12-13 0.0 14-15 0.0 16-17 2.988978143097328E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0023725014010835045 40-41 0.007024098636278722 42-43 0.002465906968055296 44-45 0.0013263590509994395 46-47 0.0069493741827012885 48-49 0.007883429852419204 50-51 0.004969176162899309 52-53 0.006781244162152064 54-55 0.018251447786288063 56-57 0.02028768914627312 58-59 0.026583224360171867 60-61 0.02421072295908836 62-63 0.010311974593685784 64-65 0.014963571828881 66-67 0.022099757145525874 68-69 0.025761255370820104 70-71 0.02708761442181954 72-73 0.0211470203624136 74-75 0.03108537268821222 76-77 0.027928264524565666 78-79 0.0200261535587521 80-81 0.008966934429291985 82-83 0.0015131701849430226 84-85 0.007098823089856156 86-87 0.02566784980384831 88-89 0.023874462917989912 90-91 0.020586586960582853 92-93 0.04315337194096768 94-95 0.04470390435269942 96-97 0.032673267326732675 98-99 0.040257799364842146 100-101 0.03990285821034934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2676500.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.464507027597804 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06603156723486 25.821672269743246 2 7.920506870562469 4.98429688181899 3 2.6995012570355836 2.5481542881901573 4 1.4975641590779403 1.884804720303458 5 1.0053215933838986 1.5815974170011748 6 0.7406997738394071 1.3983451943586076 7 0.5089333674026262 1.1209336260653262 8 0.338379502727446 0.8517555393250218 9 0.3444433480851412 0.9753966131790817 >10 2.2731899128676956 14.335527142747889 >50 0.3098885643491084 6.896382809628785 >100 0.2633910854509985 16.419122541296833 >500 0.02166698945493469 4.564747836595097 >1k 0.009415073528891834 4.742638927783498 >5k 4.74193332983633E-4 0.9468396651591601 >10k+ 5.927416660367441E-4 10.927784526803856 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 213739 7.9857649915934985 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 28960 1.0820100878012329 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 26927 1.006052680739772 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12727 0.4755090603399963 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10612 0.39648795068186066 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9287 0.3469830001868111 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5543 0.20709882308985617 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5387 0.20127031571081636 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5167 0.1930506258172987 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3743 0.13984681487016626 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3310 0.12366897067065197 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3055 0.11414160283952923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00407248271997011 0.0 0.0 2 0.00452082944143471 0.0 0.0 3 0.004894451709321876 0.0 0.0 4 0.005268073977209042 0.0 0.0 5 0.005903231832617224 0.0 0.0 6 0.00638894078087054 0.0 0.0 7 0.007472445357743321 0.0 0.0 8 0.008107603213151503 0.0 0.0 9 0.008966934429291985 0.0 0.0 10-11 0.014944890715486644 0.0 0.0 12-13 0.019484401270315713 0.0 0.0 14-15 0.02542499532972165 0.0 0.0 16-17 0.031272183822155804 0.0 0.0 18-19 0.03822155800485709 0.0 0.0 20-21 0.043433588641883056 0.0 0.0 22-23 0.047861012516345974 0.0 0.0 24-25 0.053203810947132446 0.0 0.0 26-27 0.0594059405940594 0.0 0.0 28-29 0.06631795254997197 0.0 0.0 30-31 0.11858770782738651 0.0 0.0 32-33 0.12897440687464973 0.0 0.0 34-35 0.13710069120119558 0.0 0.0 36-37 0.1456940033626004 0.0 0.0 38-39 0.1558752101625257 0.0 0.0 40-41 0.16646740145712685 0.0 0.0 42-43 0.17842331402951617 0.0 0.0 44-45 0.19133196338501773 0.0 0.0 46-47 0.2031757892770409 0.0 0.0 48-49 0.2182327666728937 0.0 0.0 50-51 0.2358490566037736 0.0 0.0 52-53 0.25266205865869606 0.0 0.0 54-55 0.2717728376611246 0.0 0.0 56-57 0.29392863814683357 0.0 0.0 58-59 0.3201195591257239 0.0 0.0 60-61 0.3466280590323183 0.0 0.0 62-63 0.3764804782365029 0.0 0.0 64-65 0.40810760321315154 0.0 0.0 66-67 0.44171492620960207 0.0 0.0 68-69 0.4782925462357557 0.0 0.0 70-71 0.5198953857649916 0.0 0.0 72-73 0.5680552960956473 0.0 0.0 74-75 0.6192789090229778 0.0 0.0 76-77 0.6773771716794321 0.0 0.0 78-79 0.7458434522697552 0.0 0.0 80-81 0.8204371380534279 0.0 0.0 82-83 0.9035494115449281 0.0 0.0 84-85 0.997029702970297 0.0 0.0 86-87 1.1000747244535773 0.0 0.0 88-89 1.2126844759947693 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 29365 0.0 81.1915 3 CGGAAGA 29265 0.0 81.04698 4 GAAGAGC 29340 0.0 80.77203 6 GATCGGA 29550 0.0 80.768295 1 ATCGGAA 29535 0.0 80.41864 2 GGAAGAG 31045 0.0 76.45839 5 GAGCACA 38105 0.0 62.080494 9 AGAGCAC 38085 0.0 62.038277 8 AAGAGCA 38310 0.0 61.68631 7 ATCGGAC 175 0.0 51.564686 2 CGGACCG 40 5.3439464E-4 47.5818 1 GCGCACA 235 0.0 46.481544 9 TCGTATG 25640 0.0 45.756443 44-45 ATGCCGT 25325 0.0 45.739487 48-49 GCCGTCT 25435 0.0 45.71061 50-51 TCTCGTA 25065 0.0 45.525295 42-43 CTGCTTG 25590 0.0 45.39157 58-59 GTATGCC 25770 0.0 45.37065 46-47 AATCTCG 25020 0.0 45.36819 40-41 CGTATGC 25530 0.0 45.2297 46-47 >>END_MODULE