FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676500
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2137397.9857649915934985TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA289601.0820100878012329No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC269271.006052680739772No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC127270.4755090603399963No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT106120.39648795068186066No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT92870.3469830001868111No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC55430.20709882308985617No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT53870.20127031571081636No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT51670.1930506258172987TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT37430.13984681487016626No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33100.12366897067065197No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG30550.11414160283952923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG293650.081.19153
CGGAAGA292650.081.046984
GAAGAGC293400.080.772036
GATCGGA295500.080.7682951
ATCGGAA295350.080.418642
GGAAGAG310450.076.458395
GAGCACA381050.062.0804949
AGAGCAC380850.062.0382778
AAGAGCA383100.061.686317
ATCGGAC1750.051.5646862
CGGACCG405.3439464E-447.58181
GCGCACA2350.046.4815449
TCGTATG256400.045.75644344-45
ATGCCGT253250.045.73948748-49
GCCGTCT254350.045.7106150-51
TCTCGTA250650.045.52529542-43
CTGCTTG255900.045.3915758-59
GTATGCC257700.045.3706546-47
AATCTCG250200.045.3681940-41
CGTATGC255300.045.229746-47
Produced by FastQC (version 0.11.2)