##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_30245-1.34100000035771.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256485 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68521129101235 33.0 33.0 33.0 33.0 33.0 2 32.704120346468066 33.0 33.0 33.0 33.0 33.0 3 32.759203362752245 33.0 33.0 33.0 33.0 33.0 4 36.71462562348077 37.0 37.0 37.0 37.0 37.0 5 36.67264351413814 37.0 37.0 37.0 37.0 37.0 6 36.583512852954925 37.0 37.0 37.0 37.0 37.0 7 36.554324978894165 37.0 37.0 37.0 37.0 37.0 8 36.5018672847371 37.0 37.0 37.0 37.0 37.0 9 36.506273252425785 37.0 37.0 37.0 37.0 37.0 10-11 36.469633079767874 37.0 37.0 37.0 37.0 37.0 12-13 36.45377057680419 37.0 37.0 37.0 37.0 37.0 14-15 38.37120920369513 40.0 37.0 40.0 37.0 40.0 16-17 38.369416371037254 40.0 37.0 40.0 37.0 40.0 18-19 38.38376678772515 40.0 37.0 40.0 37.0 40.0 20-21 38.33801088861659 40.0 37.0 40.0 37.0 40.0 22-23 38.238937329519146 40.0 37.0 40.0 37.0 40.0 24-25 38.22145106216084 40.0 37.0 40.0 37.0 40.0 26-27 38.159683534346556 40.0 37.0 40.0 37.0 40.0 28-29 38.04857887377935 40.0 37.0 40.0 37.0 40.0 30-31 38.014379887302596 40.0 37.0 40.0 37.0 40.0 32-33 37.90769072251754 40.0 37.0 40.0 33.0 40.0 34-35 37.85444419085436 40.0 37.0 40.0 33.0 40.0 36-37 37.79676177772066 40.0 37.0 40.0 33.0 40.0 38-39 37.67726065096821 40.0 37.0 40.0 33.0 40.0 40-41 37.582102030370244 40.0 37.0 40.0 33.0 40.0 42-43 37.46973390029183 40.0 37.0 40.0 33.0 40.0 44-45 37.355787873617594 40.0 37.0 40.0 33.0 40.0 46-47 37.331036324194486 40.0 37.0 40.0 33.0 40.0 48-49 37.12674402887677 40.0 37.0 40.0 33.0 40.0 50-51 37.01640538270806 38.5 37.0 40.0 33.0 40.0 52-53 36.92212999421667 37.0 37.0 40.0 33.0 40.0 54-55 36.66616972858229 37.0 37.0 40.0 33.0 40.0 56-57 36.55271295842871 37.0 37.0 40.0 33.0 40.0 58-59 36.38838037921812 37.0 37.0 40.0 33.0 40.0 60-61 36.149993463284716 37.0 37.0 40.0 33.0 40.0 62-63 35.891727399029904 37.0 37.0 40.0 33.0 40.0 64-65 35.69539682293478 37.0 37.0 40.0 30.0 40.0 66-67 35.3686421580467 37.0 33.0 38.5 27.0 40.0 68-69 35.14317866061596 37.0 33.0 37.0 27.0 40.0 70-71 34.77979490224841 37.0 33.0 37.0 27.0 40.0 72-73 34.492022548343996 37.0 33.0 37.0 27.0 40.0 74-75 34.06251027593802 37.0 33.0 37.0 27.0 38.5 76-77 31.470346800444055 33.0 30.0 35.0 24.5 37.0 78-79 33.652382134159986 37.0 33.0 37.0 27.0 37.0 80-81 33.956853247417996 37.0 33.0 37.0 27.0 37.0 82-83 33.906258849493796 37.0 33.0 37.0 27.0 37.0 84-85 33.752734673618484 37.0 33.0 37.0 27.0 37.0 86-87 33.60293354487178 37.0 33.0 37.0 27.0 37.0 88-89 33.37364307761851 37.0 33.0 37.0 27.0 37.0 90-91 33.116547196192315 37.0 33.0 37.0 27.0 37.0 92-93 33.10943015353526 37.0 33.0 37.0 27.0 37.0 94-95 33.04550307225619 37.0 33.0 37.0 27.0 37.0 96-97 32.88801232890979 35.0 33.0 37.0 27.0 37.0 98-99 32.734705748099366 33.0 33.0 37.0 27.0 37.0 100-101 31.194313944032423 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.13842527657274672 2306 2 0.1271737855347581 2306 3 0.11097057593364212 2306 4 0.12104402614535559 2306 5 0.15458076743499305 2306 6 0.1322524366834159 2306 7 0.11979496866380401 2306 8 0.11342199093785155 2306 9 0.17374954768860107 2306 10-11 0.16916368881292243 2306 12-13 0.1611159097177577 2306 14-15 0.3841507162019653 2306 16-17 0.3062376532884983 2306 18-19 0.24514006746479566 2306 20-21 0.3603455516601386 2306 22-23 0.40365887799162437 2306 24-25 0.3509029603496501 2306 26-27 0.40026752645020736 2306 28-29 0.436102521735414 2306 30-31 0.38908877584304236 2306 32-33 0.42067986812632086 2306 34-35 0.3008757921382639 2306 36-37 0.3267265872666556 2306 38-39 0.3140181669480455 2306 40-41 0.3864141659388096 2306 42-43 0.25442881677057727 2306 44-45 0.34004050247126116 2306 46-47 0.29215671324679704 2306 48-49 0.4803666553851471 2306 50-51 0.39821603086312507 2306 52-53 0.13371709257271647 2306 54-55 0.3444301795264053 2306 56-57 0.42019578662884527 2306 58-59 0.37278794381862923 2306 60-61 0.3459925377380486 2306 62-63 0.37588073313484216 2306 64-65 0.25304772448799895 2306 66-67 0.2573135613853381 2306 68-69 0.41041786647493694 2306 70-71 0.41428854555844197 2306 72-73 0.34636200431125985 2306 74-75 0.2798971317888288 2306 76-77 -0.06712694701157673 2306 78-79 0.4445949378749603 2306 80-81 0.4392819068757845 2306 82-83 0.32098473254291804 2306 84-85 0.3993103451451745 2306 86-87 0.33092024623541505 2306 88-89 0.44937705353580526 2306 90-91 0.4503744867775268 2306 92-93 0.29471754625362934 2306 94-95 0.2998596714554651 2306 96-97 0.24754417516645333 2306 98-99 0.25333773911795276 2306 100-101 0.22554454408425073 2307 1 0.13229705532824454 2307 2 0.08652779989183301 2307 3 0.0956886171082374 2307 4 0.11379036795840847 2307 5 0.13510995022856065 2307 6 0.1839184693011049 2307 7 0.1387282226031843 2307 8 0.16907426193590425 2307 9 0.1517266833309776 2307 10-11 0.2182687148916358 2307 12-13 0.18166254264199466 2307 14-15 0.2245257837419672 2307 16-17 0.29856221469201216 2307 18-19 0.24032646408173264 2307 20-21 0.2735118544378281 2307 22-23 0.27902380074792177 2307 24-25 0.33096970089680866 2307 26-27 0.27069582653732027 2307 28-29 0.3709980966248665 2307 30-31 0.3073205786372668 2307 32-33 0.2509356517557322 2307 34-35 0.33945663889689826 2307 36-37 0.27887518629408703 2307 38-39 0.1874661253103298 2307 40-41 0.2242902534706488 2307 42-43 0.13087575789774775 2307 44-45 0.11770502439832597 2307 46-47 0.12204757411990386 2307 48-49 0.347846972299358 2307 50-51 0.11816417668336499 2307 52-53 -0.018340603127875 2307 54-55 0.1218028321668072 2307 56-57 0.2450118435058002 2307 58-59 0.3344676084894189 2307 60-61 0.13097963621964226 2307 62-63 0.16029374161455934 2307 64-65 0.101903197157732 2307 66-67 0.02006518234534127 2307 68-69 0.1285379525953232 2307 70-71 0.15638085206322927 2307 72-73 0.13273642953208054 2307 74-75 0.15174756239078846 2307 76-77 -0.2785223234161336 2307 78-79 0.1339026666563754 2307 80-81 0.24059197612496064 2307 82-83 0.1852641358752578 2307 84-85 0.20825967084787322 2307 86-87 0.24351073605203055 2307 88-89 0.3530022975068263 2307 90-91 0.40974297399178994 2307 92-93 0.24615869823792735 2307 94-95 0.20105037409200577 2307 96-97 0.17400104053855614 2307 98-99 -0.015729905638188768 2307 100-101 0.19671771388808068 2201 1 0.17265285615525272 2201 2 0.21504866368756836 2201 3 0.11625958074202458 2201 4 0.12548891205224066 2201 5 0.19624307288000864 2201 6 0.12306147356191133 2201 7 0.1338227936139802 2201 8 0.052590959261500814 2201 9 0.10456597550273017 2201 10-11 0.19357353541897027 2201 12-13 0.1490294514356023 2201 14-15 0.3005368406103557 2201 16-17 0.23169921294680762 2201 18-19 0.30250366091207326 2201 20-21 0.2460475445782322 2201 22-23 0.3595806337589451 2201 24-25 0.2998184580309058 2201 26-27 0.20256765255182074 2201 28-29 0.26922420231170463 2201 30-31 0.3346158713121383 2201 32-33 0.37297735969404044 2201 34-35 0.37932651531782113 2201 36-37 0.283646973788386 2201 38-39 0.34726794283465523 2201 40-41 0.37236355583059577 2201 42-43 0.36106193229203853 2201 44-45 0.3069010921938755 2201 46-47 0.3065661895940224 2201 48-49 0.42232164330544464 2201 50-51 0.3452613654952117 2201 52-53 0.41527151029444553 2201 54-55 0.48839766652996985 2201 56-57 0.46518276704714623 2201 58-59 0.4720520188159796 2201 60-61 0.39485673321192394 2201 62-63 0.41639310013538733 2201 64-65 0.447314871921499 2201 66-67 0.37478066353332906 2201 68-69 0.4706596882052807 2201 70-71 0.43600013102805946 2201 72-73 0.39088954337891124 2201 74-75 0.40934940507116124 2201 76-77 0.2248039740855603 2201 78-79 0.5335772103099785 2201 80-81 0.5728871312277093 2201 82-83 0.544157879094918 2201 84-85 0.475728383015543 2201 86-87 0.5043676335254261 2201 88-89 0.7697269409984813 2201 90-91 0.7798866779051252 2201 92-93 0.642508418376913 2201 94-95 0.5327814834736344 2201 96-97 0.5634682236903004 2201 98-99 0.4986512949341062 2201 100-101 0.508430113460431 1101 1 0.13250773371264302 1101 2 0.13824558114747987 1101 3 0.06878448580543761 1101 4 0.1127432462264295 1101 5 0.16508145848514744 1101 6 0.17357641789557476 1101 7 0.08960002982242798 1101 8 0.15239681054241316 1101 9 0.16583586489561242 1101 10-11 0.0902602777239565 1101 12-13 0.15254252618901631 1101 14-15 0.2608759562975962 1101 16-17 0.21109933421688254 1101 18-19 0.28813101975098476 1101 20-21 0.2710426536111612 1101 22-23 0.22859454353970676 1101 24-25 0.252315183350305 1101 26-27 0.27712089024245046 1101 28-29 0.3081682846302414 1101 30-31 0.316679464248935 1101 32-33 0.3185928830404592 1101 34-35 0.3362406584398272 1101 36-37 0.4154549732079502 1101 38-39 0.41018829068282514 1101 40-41 0.40893292788225466 1101 42-43 0.3310007854865873 1101 44-45 0.39083575535199344 1101 46-47 0.5714258220783321 1101 48-49 0.4209486095713757 1101 50-51 0.20902299206890262 1101 52-53 0.35029452699110664 1101 54-55 0.40758868762137723 1101 56-57 0.3556136061816133 1101 58-59 0.337450337878046 1101 60-61 0.30512789360843584 1101 62-63 0.45499643428495773 1101 64-65 0.2408449646490496 1101 66-67 0.30233847735527064 1101 68-69 0.314197651826845 1101 70-71 0.3157866386961885 1101 72-73 0.21906565965306868 1101 74-75 0.27986510086467575 1101 76-77 0.08061534989192154 1101 78-79 0.3127962038461263 1101 80-81 0.3952894770780304 1101 82-83 0.3630835895159876 1101 84-85 0.422179743481756 1101 86-87 0.31605244535598587 1101 88-89 0.3859019844989362 1101 90-91 0.3000397295446646 1101 92-93 0.40751137886956457 1101 94-95 0.3709267510257348 1101 96-97 0.4178042117498606 1101 98-99 0.24937749017632882 1101 100-101 0.3360607735401615 2304 1 0.1205903435710809 2304 2 0.1097452024608998 2304 3 0.10003062308999944 2304 4 0.06441070322808429 2304 5 0.11968963586777903 2304 6 0.07119619859447113 2304 7 0.08090147557361149 2304 8 0.08243811771902898 2304 9 0.0484486449495094 2304 10-11 0.06016958397464123 2304 12-13 0.10901604397380282 2304 14-15 0.18788833271879213 2304 16-17 0.2497689082675194 2304 18-19 0.12326133512835469 2304 20-21 0.13558185834688175 2304 22-23 0.21006277993902245 2304 24-25 0.10679503430286985 2304 26-27 0.16393799024491784 2304 28-29 0.1147538352090649 2304 30-31 0.09605829465802884 2304 32-33 0.2177760100251973 2304 34-35 0.2923618965763737 2304 36-37 0.2358187595343324 2304 38-39 0.3322909450846865 2304 40-41 0.2976847567024379 2304 42-43 0.18015570762548094 2304 44-45 0.2751927972367554 2304 46-47 0.3302757947724473 2304 48-49 0.37115301042629056 2304 50-51 0.20604462777017574 2304 52-53 0.3380456456334997 2304 54-55 0.4218509567293083 2304 56-57 0.29909856771740095 2304 58-59 0.2919483927550175 2304 60-61 0.32171312955152587 2304 62-63 0.3540607300964851 2304 64-65 0.3514773487563545 2304 66-67 0.3351091933088739 2304 68-69 0.18092526043798784 2304 70-71 0.28353310522456354 2304 72-73 0.31981370610229476 2304 74-75 0.3327368261247514 2304 76-77 0.1307188425732697 2304 78-79 0.23216995513556782 2304 80-81 0.3613377534360751 2304 82-83 0.3039303866366154 2304 84-85 0.3486555536125735 2304 86-87 0.30904281076044526 2304 88-89 0.3583528170475532 2304 90-91 0.45824026038008725 2304 92-93 0.3007908720363517 2304 94-95 0.4182850469586157 2304 96-97 0.39772258625178125 2304 98-99 0.3014086885564069 2304 100-101 0.4436043899182991 2202 1 0.14237481540600783 2202 2 0.14591942178345363 2202 3 0.11331400973362804 2202 4 0.04448411115215123 2202 5 0.11376417888278922 2202 6 0.016590864473499778 2202 7 0.1068366907116527 2202 8 0.15175880712044432 2202 9 0.08011979922409296 2202 10-11 0.08877039789332031 2202 12-13 0.055146603626049284 2202 14-15 0.11646858193476817 2202 16-17 0.1730419016716951 2202 18-19 0.16332041832568933 2202 20-21 0.1407386591646258 2202 22-23 0.04563134860546825 2202 24-25 0.2220397794668898 2202 26-27 0.18650271109420657 2202 28-29 0.1303794810062513 2202 30-31 0.14078047459715748 2202 32-33 0.19863294320301605 2202 34-35 0.26422303241329104 2202 36-37 0.3083254657443675 2202 38-39 0.15577371861792244 2202 40-41 0.11494267537306513 2202 42-43 0.16913620240818972 2202 44-45 0.002911597104898078 2202 46-47 0.11819725776959444 2202 48-49 0.2978884848408754 2202 50-51 0.23173135275054335 2202 52-53 0.12472944619216975 2202 54-55 0.11779023440421099 2202 56-57 0.2766505313944876 2202 58-59 0.3657282960001069 2202 60-61 0.26449814473814826 2202 62-63 0.08703310585392643 2202 64-65 0.27033245933699135 2202 66-67 0.236788492818647 2202 68-69 0.3911635589709306 2202 70-71 0.36532618600856637 2202 72-73 0.2675188768145276 2202 74-75 0.2223368829801089 2202 76-77 -0.014472746866555752 2202 78-79 0.30426158701529715 2202 80-81 0.47068929997228537 2202 82-83 0.44653299089412 2202 84-85 0.41828219337039485 2202 86-87 0.15743155622285343 2202 88-89 0.40529628730081413 2202 90-91 0.5631368773856948 2202 92-93 0.3688212271504554 2202 94-95 0.4201598119514358 2202 96-97 0.3549633777508987 2202 98-99 0.17343172102343374 2202 100-101 0.32937981530018234 1102 1 0.14133663789723983 1102 2 0.14503795031374977 1102 3 0.1065201499985875 1102 4 0.06161404004678417 1102 5 0.16013610760765573 1102 6 0.10052653664446609 1102 7 0.1223407944510484 1102 8 0.12151454158206576 1102 9 0.15802228889128855 1102 10-11 0.14039361128123318 1102 12-13 0.10517174188648681 1102 14-15 0.2832282928121259 1102 16-17 0.19357855456128448 1102 18-19 0.27235354206469253 1102 20-21 0.2033361923503989 1102 22-23 0.16402602582367365 1102 24-25 0.19114746916280723 1102 26-27 0.1758842445933908 1102 28-29 0.3145270328008607 1102 30-31 0.2998224130508689 1102 32-33 0.33525285923469994 1102 34-35 0.3041683811821727 1102 36-37 0.28702813562350826 1102 38-39 0.3217401051725446 1102 40-41 0.34363881583391986 1102 42-43 0.25401374316808045 1102 44-45 0.3297742769087577 1102 46-47 0.4058899572269965 1102 48-49 0.40337450209160153 1102 50-51 0.27067443581127293 1102 52-53 0.3022006677810438 1102 54-55 0.5395204649579668 1102 56-57 0.4698103127895621 1102 58-59 0.45139916108274036 1102 60-61 0.41806026888851733 1102 62-63 0.3775367198869475 1102 64-65 0.359592872126818 1102 66-67 0.47494412803310837 1102 68-69 0.3760806027115038 1102 70-71 0.26730002915888207 1102 72-73 0.3011143662923672 1102 74-75 0.23402780643574062 1102 76-77 -0.086280024556185 1102 78-79 0.4462421554664573 1102 80-81 0.4379328407452334 1102 82-83 0.42136208337026204 1102 84-85 0.3653661551602383 1102 86-87 0.4209915256651797 1102 88-89 0.6026430581769944 1102 90-91 0.5532382792517936 1102 92-93 0.316411218858093 1102 94-95 0.3839656139925367 1102 96-97 0.2582120898231395 1102 98-99 0.2884506889835805 1102 100-101 0.3968662504336784 2305 1 0.075771633742022 2305 2 0.05113067451575404 2305 3 0.027171901140626176 2305 4 0.02046042669815762 2305 5 0.10110705116485974 2305 6 0.06422938124204336 2305 7 -0.05474214205521122 2305 8 -0.0019004428905304849 2305 9 -0.027526997384349272 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-1.2300090807629616 1213 88-89 -1.3035821599038897 1213 90-91 -1.082906133575456 1213 92-93 -1.2495585737642152 1213 94-95 -1.2056185939967676 1213 96-97 -1.256594863886182 1213 98-99 -1.2901348821732448 1213 100-101 -1.2099389617384269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 8.0 10 15.0 11 6.0 12 15.0 13 37.0 14 141.0 15 335.0 16 621.0 17 1096.0 18 1685.0 19 2347.0 20 3355.0 21 4401.0 22 5828.0 23 7320.0 24 9453.0 25 11965.0 26 15591.0 27 20655.0 28 29119.0 29 36118.0 30 36516.0 31 43499.0 32 58583.0 33 83700.0 34 128387.0 35 224003.0 36 450180.0 37 792906.0 38 288162.0 39 437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.22274234306547 25.825050566756108 24.155015742731972 14.797191347446445 2 29.111161829128047 24.129254127547934 33.439353685045546 13.320230358278472 3 36.55464140023089 20.013206380720458 27.414230539976998 16.01792167907165 4 28.287489152431494 20.385539648250695 34.17349727381435 17.153473925503466 5 31.145697844213455 18.97202950606807 33.80275073842724 16.079521911291234 6 25.380492225740475 19.783601486382583 38.56480322271143 16.271103065165512 7 31.503245091369987 21.221723166783736 29.566826280697633 17.708205461148648 8 28.05686720718285 28.83236538244216 26.146683891096107 16.964083519278876 9 29.843406891692165 25.710119943185973 26.332548188886694 18.11392497623516 10-11 23.970510092699406 29.3034182712272 30.02174228084769 16.704329355225703 12-13 23.164745167816317 27.70725265180136 29.886283312319822 19.241718868062495 14-15 22.199615774091118 28.389752203094638 30.62045615193542 18.790175870878823 16-17 22.001433206971218 28.45569511424441 31.329421337145764 18.213450341638605 18-19 20.62169701992258 28.448826382626073 32.35762701724142 18.57184958020993 20-21 21.483435520289298 28.831301781310316 31.958643642656607 17.72661905574378 22-23 20.60306184175831 30.59652512646882 30.932246392065537 17.868166639707333 24-25 20.744609425721862 28.707214982594607 31.706415065910033 18.841760525773495 26-27 20.69809903456039 28.900834705304934 30.78784038006014 19.61322588007454 28-29 21.181705174197923 29.762285147031776 30.64104126550808 18.41496841326222 30-31 20.05473114157639 29.38889467468208 31.327706587901094 19.228667595840435 32-33 19.90954958707902 30.264349197978273 30.862403251074127 18.963697963868583 34-35 20.141086721402253 29.370421113018946 31.71200599871171 18.77648616686709 36-37 20.90197807652167 29.279942033738315 31.015384547205056 18.80269534253496 38-39 21.452349544733675 29.940616279768033 30.32810951298436 18.278924662513933 40-41 21.344694313017285 29.969603152390427 29.968251393475082 18.717451141117206 42-43 20.188936540598178 30.03254271480607 31.20397851640143 18.57454222819432 44-45 19.95125533359451 29.224530368915445 31.54514255122644 19.279071746263604 46-47 20.974008605664704 29.127584508208 30.51751215316904 19.38089473295825 48-49 20.289906137619322 30.413906595008953 30.63881199247257 18.65737527489915 50-51 21.165223411011645 29.47747187169406 30.424307855538817 18.932996861755473 52-53 20.137861986881706 29.3278928979094 29.792367154490208 20.741877960718686 54-55 21.076965152120934 29.43528333349586 30.406412314708025 19.08133919967518 56-57 20.259151750539843 29.399521404376955 30.489160897359035 19.852165947724167 58-59 20.190785357163993 29.36304917893985 30.909816704114025 19.536348759782136 60-61 20.64412829371697 29.657815155553074 30.796347865096017 18.901708685633935 62-63 19.861414776602906 29.645350715729652 30.431020078192418 20.062214429475024 64-65 20.61042023153657 29.431078344657806 30.413104197661315 19.545397226144303 66-67 20.038334335871372 30.594660941930663 29.780832034171105 19.58617268802686 68-69 19.844600319922264 31.06668164986266 29.685811689153653 19.402906341061428 70-71 19.61089885715337 31.710386647483645 29.36473721575954 19.313977279603442 72-73 19.63573749570858 31.353145042089704 29.495750248619707 19.515367213582007 74-75 19.880756159690456 30.746362349127594 29.724246956839384 19.648634534342563 76-77 20.026385241819277 30.494503850396743 29.83411919417734 19.64499171360664 78-79 19.942615133762455 30.524684113953242 29.90074669918446 19.63195405309984 80-81 19.57107742111053 30.934494193483193 29.778376409080877 19.716051976325403 82-83 19.42853128933894 30.66877944222991 29.774114569841803 20.128574698589343 84-85 19.557603449716275 30.316737368653968 30.13380525607947 19.991853925550288 86-87 19.715920765104233 30.410339874180437 30.271323508062043 19.60241585265329 88-89 20.00533711482399 30.870865093562415 29.52887328142354 19.594924510190054 90-91 20.150324847594018 30.405907673867823 29.39424135220997 20.04952612632819 92-93 19.679164822964708 30.726989047745306 29.698469380063898 19.895376749226088 94-95 19.358914246633347 31.06978053709055 29.65000548684985 19.921299729426252 96-97 19.559063838012342 31.384089395738222 29.339493491535585 19.717353274713854 98-99 20.001542897881443 30.728784267407256 29.090630176245046 20.179042658466255 100-101 19.979929137930537 30.7125422050061 28.78865231498795 20.518876342075412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 19.0 2 17.5 3 44.0 4 60.0 5 52.5 6 47.5 7 50.0 8 49.5 9 46.0 10 49.5 11 46.5 12 52.0 13 64.5 14 79.5 15 106.5 16 151.0 17 194.5 18 275.5 19 440.5 20 715.0 21 1171.0 22 1942.0 23 3095.5 24 4601.5 25 6924.0 26 10757.0 27 16027.0 28 21894.0 29 27783.5 30 33667.5 31 39462.5 32 46361.5 33 56215.5 34 68327.0 35 84331.0 36 104786.5 37 126062.0 38 147129.5 39 158304.5 40 159744.5 41 149844.5 42 132682.0 43 122411.5 44 110825.5 45 100153.0 46 96069.5 47 86648.0 48 69926.0 49 56040.5 50 45685.5 51 37208.5 52 29991.0 53 23855.5 54 19417.0 55 15478.0 56 11402.5 57 7968.0 58 5743.5 59 4155.0 60 2989.0 61 2117.5 62 1432.0 63 966.0 64 661.5 65 474.0 66 344.0 67 239.5 68 184.5 69 117.5 70 81.5 71 62.0 72 40.0 73 33.5 74 22.5 75 12.0 76 11.5 77 8.0 78 5.5 79 5.0 80 3.5 81 2.5 82 1.5 83 1.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1780202394432048 2 0.0 3 0.0 4 0.009705360328120948 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0023044691190058876 12-13 0.0 14-15 0.0 16-17 3.102169967892541E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.215835691351815E-5 36-37 0.0 38-39 0.0025925277588816236 40-41 0.007334416138374507 42-43 0.0029249031125843953 44-45 0.0014624515562921977 46-47 0.007999166845780052 48-49 0.008996292906888368 50-51 0.005672539369860646 52-53 0.007555999707509689 54-55 0.01998683793599337 56-57 0.022579365694874995 58-59 0.028872339058314146 60-61 0.026545711582394743 62-63 0.011345078739721293 64-65 0.01699545975266842 66-67 0.024108292321907747 68-69 0.027675787784984168 70-71 0.029714356621027834 72-73 0.02346569997141572 74-75 0.03416818636064498 76-77 0.03006889033164413 78-79 0.021693031418334268 80-81 0.01005989403873724 82-83 0.002370944189746442 84-85 0.008132116987261162 86-87 0.028163271637081568 88-89 0.025903119231902715 90-91 0.022335623768826293 92-93 0.04693139994283144 94-95 0.048416009856037154 96-97 0.03576358805841829 98-99 0.0438292299749389 100-101 0.043275271052100946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2256485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.08231265748189 #Duplication Level Percentage of deduplicated Percentage of total 1 92.39212660767565 61.054853975780745 2 3.873903674853936 5.119930276933317 3 1.00789072368298 1.998112497809831 4 0.6732028802694827 1.7794721286354125 5 0.36563927792752327 1.2081144541931255 6 0.28453017791666724 1.1281447306546892 7 0.18834939958480923 0.8712594738548647 8 0.16958047616569177 0.8965016037268718 9 0.12121621877260912 0.7209223261280353 >10 0.7999512240884148 10.19574500807268 >50 0.05596081154039484 2.532479182517792 >100 0.0634435998387197 8.762200907739926 >500 0.0034008885187404784 1.442033577472281 >1k 7.371841973461738E-4 0.9078151201663786 >5k 0.0 0.0 >10k+ 6.685496698419166E-5 1.3824147363141237 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 31291 1.3867142923617928 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2586 0.11460302195671586 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2417 0.10711349731994672 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2380 0.1054737789083464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.647507074055445E-4 0.0 0.0 2 7.090674212325808E-4 0.0 0.0 3 7.090674212325808E-4 0.0 0.0 4 7.977008488866534E-4 0.0 0.0 5 9.306509903677623E-4 0.0 0.0 6 0.0011522345595029438 0.0 4.43167138270363E-5 7 0.0014181348424651616 0.0 4.43167138270363E-5 8 0.0015954016977733068 0.0 4.43167138270363E-5 9 0.0017283518392544157 0.0 4.43167138270363E-5 10-11 0.0024595776174005145 0.0 4.43167138270363E-5 12-13 0.0036339705338169766 0.0 4.43167138270363E-5 14-15 0.004830521807146957 0.0 4.43167138270363E-5 16-17 0.006292973363439154 0.0 6.647507074055445E-5 18-19 0.007977008488866533 0.0 8.86334276540726E-5 20-21 0.009661043614293912 0.0 8.86334276540726E-5 22-23 0.012209254659348499 0.0 8.86334276540726E-5 24-25 0.014447248707613833 0.0 8.86334276540726E-5 26-27 0.017660210460073964 0.0 1.329501414811089E-4 28-29 0.02047432178809077 0.0 1.329501414811089E-4 30-31 0.030334790614606345 0.0 1.329501414811089E-4 32-33 0.03469998692656942 0.0 1.329501414811089E-4 34-35 0.03904302488161898 0.0 1.329501414811089E-4 36-37 0.0435854880488902 0.0 1.329501414811089E-4 38-39 0.048327376428383084 0.0 1.329501414811089E-4 40-41 0.0533573234477517 0.0 1.329501414811089E-4 42-43 0.059184971316006973 0.0 1.329501414811089E-4 44-45 0.06503477754117576 0.0 1.329501414811089E-4 46-47 0.07130559254770141 0.0 1.329501414811089E-4 48-49 0.07649064806546466 0.0 1.772668553081452E-4 50-51 0.08256203785976862 0.0 2.215835691351815E-4 52-53 0.08923170329073758 0.0 2.215835691351815E-4 54-55 0.09700928656738245 0.0 2.4374192604869965E-4 56-57 0.10518572026847064 0.0 2.8805863987573595E-4 58-59 0.11369452932326163 0.0 3.102169967892541E-4 60-61 0.1240424820018746 0.0 3.3237535370277225E-4 62-63 0.1360301530920879 0.0 3.988504244433267E-4 64-65 0.14808429925304178 0.0 3.988504244433267E-4 66-67 0.16100262133362286 0.0 3.988504244433267E-4 68-69 0.17531691989975562 0.0 3.988504244433267E-4 70-71 0.19107151166526698 0.0 3.988504244433267E-4 72-73 0.20910841419287077 0.0 3.988504244433267E-4 74-75 0.22840834306454508 0.0 3.988504244433267E-4 76-77 0.25180756796522025 0.0 3.988504244433267E-4 78-79 0.2802810565990911 0.0 3.988504244433267E-4 80-81 0.3099510965062918 0.0 3.988504244433267E-4 82-83 0.34524935906952625 0.0 3.988504244433267E-4 84-85 0.38165553947843656 0.0 3.988504244433267E-4 86-87 0.42320245869128315 0.0 3.988504244433267E-4 88-89 0.47048839234473083 0.0 3.988504244433267E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 5485 0.0 68.06066 3 CGGAAGA 5520 0.0 67.543076 4 GATCGGA 5765 0.0 64.625305 1 ATCGGAA 5945 0.0 62.474834 2 GAAGAGC 6275 0.0 59.63948 6 GGAAGAG 7490 0.0 50.0918 5 TATGCCG 4105 0.0 43.505497 48-49 GCCGTCT 4150 0.0 43.320843 50-51 TTGTGTT 24670 0.0 43.29995 1 ATGCCGT 4175 0.0 43.174248 48-49 CGTATGC 4185 0.0 42.842197 46-47 TCGTATG 4285 0.0 42.673214 44-45 TGCCGTC 4180 0.0 42.555397 50-51 AATCTCG 4160 0.0 42.128273 40-41 CACTCCG 4370 0.0 41.94768 30-31 TCTCGTA 4265 0.0 41.53655 42-43 CGTCTTC 4320 0.0 41.508896 52-53 CCGTCTT 4290 0.0 41.46699 52-53 GTATGCC 4405 0.0 41.241623 46-47 CTCGTAT 4255 0.0 40.964905 44-45 >>END_MODULE