FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256485
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT312911.3867142923617928TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25860.11460302195671586No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC24170.10711349731994672No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG23800.1054737789083464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG54850.068.060663
CGGAAGA55200.067.5430764
GATCGGA57650.064.6253051
ATCGGAA59450.062.4748342
GAAGAGC62750.059.639486
GGAAGAG74900.050.09185
TATGCCG41050.043.50549748-49
GCCGTCT41500.043.32084350-51
TTGTGTT246700.043.299951
ATGCCGT41750.043.17424848-49
CGTATGC41850.042.84219746-47
TCGTATG42850.042.67321444-45
TGCCGTC41800.042.55539750-51
AATCTCG41600.042.12827340-41
CACTCCG43700.041.9476830-31
TCTCGTA42650.041.5365542-43
CGTCTTC43200.041.50889652-53
CCGTCTT42900.041.4669952-53
GTATGCC44050.041.24162346-47
CTCGTAT42550.040.96490544-45
Produced by FastQC (version 0.11.2)