##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236066 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66347415505625 33.0 33.0 33.0 33.0 33.0 2 32.672459131349434 33.0 33.0 33.0 33.0 33.0 3 32.726466481758585 33.0 33.0 33.0 33.0 33.0 4 36.675263163073005 37.0 37.0 37.0 37.0 37.0 5 36.634246037460436 37.0 37.0 37.0 37.0 37.0 6 36.543447286439665 37.0 37.0 37.0 37.0 37.0 7 36.4968592161412 37.0 37.0 37.0 37.0 37.0 8 36.42682058579666 37.0 37.0 37.0 37.0 37.0 9 36.43082270380212 37.0 37.0 37.0 37.0 37.0 10-11 36.37279981896778 37.0 37.0 37.0 37.0 37.0 12-13 36.345865238324805 37.0 37.0 37.0 37.0 37.0 14-15 38.16751920560485 40.0 37.0 40.0 37.0 40.0 16-17 38.11660009141054 40.0 37.0 40.0 37.0 40.0 18-19 38.147337109011985 40.0 37.0 40.0 37.0 40.0 20-21 38.08743681984342 40.0 37.0 40.0 37.0 40.0 22-23 37.95337659979625 40.0 37.0 40.0 33.0 40.0 24-25 37.94652528145413 40.0 37.0 40.0 33.0 40.0 26-27 37.83402658955505 40.0 37.0 40.0 33.0 40.0 28-29 37.699694910615335 40.0 37.0 40.0 33.0 40.0 30-31 37.662342032838026 40.0 37.0 40.0 33.0 40.0 32-33 37.57559951271564 40.0 37.0 40.0 33.0 40.0 34-35 37.45165437871691 40.0 37.0 40.0 33.0 40.0 36-37 37.345114589640914 40.0 37.0 40.0 33.0 40.0 38-39 37.21974507908085 40.0 37.0 40.0 33.0 40.0 40-41 37.119636898016424 38.5 37.0 40.0 33.0 40.0 42-43 36.899544110057576 37.0 37.0 40.0 33.0 40.0 44-45 36.75018648823425 37.0 37.0 40.0 33.0 40.0 46-47 36.73169709659733 37.0 37.0 40.0 33.0 40.0 48-49 36.503921395880084 37.0 37.0 40.0 33.0 40.0 50-51 36.38179687003872 37.0 37.0 40.0 33.0 40.0 52-53 36.271492657193484 37.0 37.0 40.0 33.0 40.0 54-55 35.966850262917106 37.0 37.0 40.0 33.0 40.0 56-57 35.7966509038642 37.0 35.0 40.0 33.0 40.0 58-59 35.59388698723562 37.0 33.0 40.0 27.0 40.0 60-61 35.37424610901467 37.0 33.0 40.0 27.0 40.0 62-63 35.064649478145995 37.0 33.0 37.0 27.0 40.0 64-65 34.807328808720314 37.0 33.0 37.0 27.0 40.0 66-67 34.34281590972717 37.0 33.0 37.0 27.0 40.0 68-69 34.04223354766809 37.0 33.0 37.0 27.0 38.5 70-71 33.621305229809856 37.0 33.0 37.0 27.0 37.0 72-73 33.202599565486885 37.0 33.0 37.0 27.0 37.0 74-75 32.689086323927825 35.0 33.0 37.0 24.5 37.0 76-77 29.994152006246686 33.0 30.0 33.0 22.0 37.0 78-79 32.32023070875368 33.0 33.0 37.0 24.5 37.0 80-81 32.80610008828005 37.0 33.0 37.0 27.0 37.0 82-83 32.67624569221123 37.0 33.0 37.0 24.5 37.0 84-85 32.58915971174375 35.0 33.0 37.0 24.5 37.0 86-87 32.490878623439556 33.0 33.0 37.0 22.0 37.0 88-89 32.34082625468121 33.0 33.0 37.0 22.0 37.0 90-91 32.12827438903861 33.0 33.0 37.0 22.0 37.0 92-93 32.09463987198947 33.0 33.0 37.0 22.0 37.0 94-95 31.95544675336059 33.0 33.0 37.0 22.0 37.0 96-97 31.777988216805767 33.0 33.0 37.0 22.0 37.0 98-99 31.600687994003756 33.0 33.0 37.0 18.5 37.0 100-101 29.899188127720738 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.17074463582978439 2306 2 0.21901580337702597 2306 3 0.1645671536673774 2306 4 0.1603360511156282 2306 5 0.20897820512692533 2306 6 0.19408976348446316 2306 7 0.19580209661575054 2306 8 0.2214935497862882 2306 9 0.20319285797989295 2306 10-11 0.2063840882906547 2306 12-13 0.23504545978946823 2306 14-15 0.38187172493155686 2306 16-17 0.4157661122933476 2306 18-19 0.38110259611689656 2306 20-21 0.44287428833267484 2306 22-23 0.5007942409698387 2306 24-25 0.4566132798666729 2306 26-27 0.44228549095264214 2306 28-29 0.49022216439429656 2306 30-31 0.45056196289818473 2306 32-33 0.5404412279816313 2306 34-35 0.3624350925384192 2306 36-37 0.5319298017060277 2306 38-39 0.5406928906720196 2306 40-41 0.40553816459609493 2306 42-43 0.40515325403234215 2306 44-45 0.49665173270634 2306 46-47 0.6037203322566285 2306 48-49 0.6754112287213232 2306 50-51 0.4413370561639738 2306 52-53 0.32634605448346576 2306 54-55 0.4948621201424217 2306 56-57 0.45432582716565406 2306 58-59 0.6362950519579442 2306 60-61 0.6596004393126549 2306 62-63 0.5732215297644814 2306 64-65 0.4462240708862666 2306 66-67 0.3744016300893378 2306 68-69 0.7159135963106493 2306 70-71 0.5456034090984829 2306 72-73 0.5176727717493534 2306 74-75 0.4591123357489906 2306 76-77 5.089526646173681E-5 2306 78-79 0.6004818587920937 2306 80-81 0.6586076824136597 2306 82-83 0.5827496981528739 2306 84-85 0.8284294493743829 2306 86-87 0.5903052471166532 2306 88-89 0.8904708244040194 2306 90-91 0.7572438920893347 2306 92-93 0.5911329934030434 2306 94-95 0.4978004767911983 2306 96-97 0.5329795323296231 2306 98-99 0.4492316546618298 2306 100-101 0.4395881266539412 2307 1 0.16990214849801788 2307 2 0.1683122878087815 2307 3 0.08291018977530484 2307 4 0.07498450975027282 2307 5 0.15125097315383584 2307 6 0.19284844728737482 2307 7 0.14923063359398725 2307 8 0.19365900502315725 2307 9 0.16559133439179163 2307 10-11 0.1534075573597704 2307 12-13 0.22407565639045401 2307 14-15 0.35512406611882597 2307 16-17 0.344767308779943 2307 18-19 0.41626291604567456 2307 20-21 0.3195873201431425 2307 22-23 0.33080518557591176 2307 24-25 0.3995529268567495 2307 26-27 0.49644382292047595 2307 28-29 0.4551654163026484 2307 30-31 0.4225354237743062 2307 32-33 0.3870607668860018 2307 34-35 0.37741096184621625 2307 36-37 0.35041644712243425 2307 38-39 0.45284202175067634 2307 40-41 0.34172879242692744 2307 42-43 0.31204340914275974 2307 44-45 0.2751276499354418 2307 46-47 0.4983658228625103 2307 48-49 0.5573439719528253 2307 50-51 0.37689605037322593 2307 52-53 0.20297050918547654 2307 54-55 0.5749263965153659 2307 56-57 0.47573675383988956 2307 58-59 0.35821332604920997 2307 60-61 0.3368126255440984 2307 62-63 0.31320304141863176 2307 64-65 0.42937834345642756 2307 66-67 0.2806096023382736 2307 68-69 0.1953558233215702 2307 70-71 0.24759739884023446 2307 72-73 0.2933635340689946 2307 74-75 0.28894643530780684 2307 76-77 0.04776056640269033 2307 78-79 0.3726381935239118 2307 80-81 0.49592786160840063 2307 82-83 0.43150049812937397 2307 84-85 0.3415799809916251 2307 86-87 0.4292870586653734 2307 88-89 0.5282131747736329 2307 90-91 0.4393080324276255 2307 92-93 0.29295168394585147 2307 94-95 0.24128689033035755 2307 96-97 0.0353691046552278 2307 98-99 0.15792736060438273 2307 100-101 0.04049337542731024 2201 1 0.09873039175590037 2201 2 0.15843341315903103 2201 3 0.09279667684736381 2201 4 0.07993170589018916 2201 5 0.14023257744255346 2201 6 0.15488149105587468 2201 7 0.11109356900258405 2201 8 0.1240521866350619 2201 9 0.13934926521829283 2201 10-11 0.15557299871922936 2201 12-13 0.12892960035717493 2201 14-15 0.24011632194305577 2201 16-17 0.23466928420354094 2201 18-19 0.25890536349799476 2201 20-21 0.24210837873830826 2201 22-23 0.3207288271245119 2201 24-25 0.3257885547498347 2201 26-27 0.3404481620011879 2201 28-29 0.28652244769217816 2201 30-31 0.3311222361310229 2201 32-33 0.20954186026406774 2201 34-35 0.2953083522493003 2201 36-37 0.32888339310644454 2201 38-39 0.11718865632806086 2201 40-41 0.3084667523563098 2201 42-43 0.3398348990401985 2201 44-45 0.35069060320395096 2201 46-47 0.5003087472623733 2201 48-49 0.46778528749651827 2201 50-51 0.28700139458646134 2201 52-53 0.10903599944010978 2201 54-55 0.5044732817171109 2201 56-57 0.3952345096392378 2201 58-59 0.3903031994780548 2201 60-61 0.299364631563769 2201 62-63 0.3916393913206946 2201 64-65 0.31979664606192415 2201 66-67 0.44440331154959267 2201 68-69 0.34176422392236816 2201 70-71 0.4492787194198442 2201 72-73 0.31271270124072004 2201 74-75 0.3715445843180376 2201 76-77 0.2679468072421649 2201 78-79 0.4867816349829184 2201 80-81 0.4742099135661064 2201 82-83 0.2975119109071258 2201 84-85 0.41100234453163864 2201 86-87 0.5794577525962268 2201 88-89 0.6612333740434266 2201 90-91 0.7352538319808346 2201 92-93 0.5525436288319128 2201 94-95 0.5676154776111701 2201 96-97 0.6375281047342831 2201 98-99 0.7261244169392356 2201 100-101 0.5977607500492539 1101 1 0.16272153501644482 1101 2 0.15104752117872522 1101 3 0.1176217988130901 1101 4 0.10558803405454142 1101 5 0.17946190794225458 1101 6 0.1898527317580161 1101 7 0.22473632061710447 1101 8 0.17275442980025701 1101 9 0.18337672394860505 1101 10-11 0.19603917823248196 1101 12-13 0.19674835811050428 1101 14-15 0.3969266732163703 1101 16-17 0.3663165460531346 1101 18-19 0.31440147571246513 1101 20-21 0.3525111726390122 1101 22-23 0.3969248662605125 1101 24-25 0.44109180857007857 1101 26-27 0.38449253957073637 1101 28-29 0.4579790560543415 1101 30-31 0.4752822075763916 1101 32-33 0.30601485534069184 1101 34-35 0.31715412120234987 1101 36-37 0.3809744524822918 1101 38-39 0.2321906456560967 1101 40-41 0.4645266712441156 1101 42-43 0.4175907460000161 1101 44-45 0.3780809759478103 1101 46-47 0.5348154020967684 1101 48-49 0.5281791307854959 1101 50-51 0.39829706658365893 1101 52-53 0.33475268965671034 1101 54-55 0.5738714949785333 1101 56-57 0.41527678490657394 1101 58-59 0.5811898667232356 1101 60-61 0.4471206750004342 1101 62-63 0.4863391828915127 1101 64-65 0.3689937652747943 1101 66-67 0.4886752747953267 1101 68-69 0.6168117467587066 1101 70-71 0.5829565148623388 1101 72-73 0.3614923071627416 1101 74-75 0.5393401741769992 1101 76-77 0.09983480780103449 1101 78-79 0.5001697580669244 1101 80-81 0.6910300231892705 1101 82-83 0.706692673422836 1101 84-85 0.5622287966455914 1101 86-87 0.5894286794811947 1101 88-89 0.6723656973766268 1101 90-91 0.690550984103588 1101 92-93 0.5929516839458486 1101 94-95 0.5454567101322354 1101 96-97 0.7938131533377408 1101 98-99 0.7488685216788582 1101 100-101 0.6374672191038755 2304 1 0.16967274722161108 2304 2 0.14426966038118394 2304 3 0.08671275632227804 2304 4 0.13060978619941466 2304 5 0.14692971246331865 2304 6 0.13045542108617525 2304 7 0.11291160372786635 2304 8 0.039100238460250125 2304 9 0.07954332771030437 2304 10-11 0.08890403941564529 2304 12-13 0.12816380692164842 2304 14-15 0.26128342170729013 2304 16-17 0.26481904082826446 2304 18-19 0.23733678875131403 2304 20-21 0.2626488244111087 2304 22-23 0.28783805177315713 2304 24-25 0.2636841828493388 2304 26-27 0.2171963964731205 2304 28-29 0.3022687787484202 2304 30-31 0.233417560970004 2304 32-33 0.28901809550126245 2304 34-35 0.3424864719549845 2304 36-37 0.36446445929856 2304 38-39 0.3526563028730152 2304 40-41 0.25962607560150985 2304 42-43 0.3288564622127552 2304 44-45 0.3707739708609594 2304 46-47 0.318594344996292 2304 48-49 0.27186575957944825 2304 50-51 0.30309820921781494 2304 52-53 0.2932264495796062 2304 54-55 0.4345365242383039 2304 56-57 0.44384750383095906 2304 58-59 0.39114436005829134 2304 60-61 0.37050370581806646 2304 62-63 0.4254712018951281 2304 64-65 0.425362035360898 2304 66-67 0.30111903801113016 2304 68-69 0.3799690417802637 2304 70-71 0.5062533820925736 2304 72-73 0.42677191504544254 2304 74-75 0.5681633664956607 2304 76-77 0.4987300958690817 2304 78-79 0.6044613704705881 2304 80-81 0.76358667734835 2304 82-83 0.5648054424824025 2304 84-85 0.555155316645525 2304 86-87 0.4560282418264947 2304 88-89 0.6897651087500165 2304 90-91 0.835608215673922 2304 92-93 0.5644832602760275 2304 94-95 0.5374223717021493 2304 96-97 0.5593416754056015 2304 98-99 0.4291483523321986 2304 100-101 0.5040817608544224 2202 1 0.13184066016028595 2202 2 0.14362792776530853 2202 3 0.10393309974848819 2202 4 0.02354376714930595 2202 5 0.14241199138667326 2202 6 0.06856828709561569 2202 7 0.08619777940091211 2202 8 0.1732656476132277 2202 9 0.1348038489081702 2202 10-11 0.15741288291206246 2202 12-13 0.15548671514958556 2202 14-15 0.1788231724146172 2202 16-17 0.16973880750835235 2202 18-19 0.09172710808444862 2202 20-21 0.11637684124205805 2202 22-23 0.06414795289658315 2202 24-25 0.09858082754210784 2202 26-27 -0.013572015548398042 2202 28-29 0.07676685558364937 2202 30-31 0.05437260644021791 2202 32-33 0.21081700859804187 2202 34-35 0.23284826037744466 2202 36-37 0.0853430613308177 2202 38-39 0.056880831314352065 2202 40-41 0.20095549778622512 2202 42-43 0.27810816084286927 2202 44-45 0.2524517990827917 2202 46-47 0.14699018806145858 2202 48-49 0.24040053069999345 2202 50-51 0.21429729247059726 2202 52-53 0.28865144376143803 2202 54-55 0.23966148749354943 2202 56-57 0.36029238646182193 2202 58-59 0.3165812728149504 2202 60-61 0.2726289577692711 2202 62-63 0.22121342677708355 2202 64-65 0.3030782469905873 2202 66-67 0.24483552374651651 2202 68-69 0.2705658291945596 2202 70-71 0.2947169766228015 2202 72-73 0.18409019026525897 2202 74-75 0.2571008816390403 2202 76-77 -0.024260220258977228 2202 78-79 0.372154402708631 2202 80-81 0.569757714996264 2202 82-83 0.5012049087177672 2202 84-85 0.2970874364990834 2202 86-87 0.24268132758451344 2202 88-89 0.47061150695096643 2202 90-91 0.5302856858362262 2202 92-93 0.43987328086744526 2202 94-95 0.3538964109509237 2202 96-97 0.13512807292248397 2202 98-99 0.00283925247759953 2202 100-101 0.13415160996952125 1102 1 0.12250666951340605 1102 2 0.13662150005931295 1102 3 0.06955389574782345 1102 4 0.05196566380311651 1102 5 0.08862890070852103 1102 6 0.059466845315682804 1102 7 0.07863152890222125 1102 8 0.11961738457349469 1102 9 0.10955673980864589 1102 10-11 0.10593292486765193 1102 12-13 0.11443123830110125 1102 14-15 0.1770431018755616 1102 16-17 0.09607797438417265 1102 18-19 0.08485988703419167 1102 20-21 0.14410293509178018 1102 22-23 0.21552816153601384 1102 24-25 0.1640407983686316 1102 26-27 0.19024853877526482 1102 28-29 0.2972925543077096 1102 30-31 0.17737159719196427 1102 32-33 0.1874362827736178 1102 34-35 0.17316605718780664 1102 36-37 0.16611968235660157 1102 38-39 0.15259635467722177 1102 40-41 0.2718432605762473 1102 42-43 0.169040552733577 1102 44-45 0.14744907141595576 1102 46-47 0.2707481316564895 1102 48-49 0.1826606521647136 1102 50-51 0.1638122932553685 1102 52-53 0.033728794492830616 1102 54-55 0.30267658381004026 1102 56-57 0.40185052840329405 1102 58-59 0.2627637261847582 1102 60-61 0.12472027272594488 1102 62-63 0.2462275260604656 1102 64-65 0.25637948443173997 1102 66-67 0.21066397735647513 1102 68-69 0.06590899780078274 1102 70-71 0.19352191974973465 1102 72-73 0.10330815308648056 1102 74-75 0.21104283845594551 1102 76-77 -0.05526354762071506 1102 78-79 0.29997540599680406 1102 80-81 0.35799638895606023 1102 82-83 0.2932283212490816 1102 84-85 0.466879453919276 1102 86-87 0.24535667669014316 1102 88-89 0.34940076858642044 1102 90-91 0.50641220532642 1102 92-93 0.2224437778605548 1102 94-95 0.24371457340924252 1102 96-97 0.19126868823894583 1102 98-99 -0.016034312155426278 1102 100-101 0.03969125125425421 2305 1 0.1480623517602382 2305 2 0.11500079047242195 2305 3 0.09106981040652329 2305 4 0.033876345569915145 2305 5 0.12470098467979085 2305 6 0.07008936014350553 2305 7 0.10129074084875356 2305 8 0.015865352661350585 2305 9 0.015681135562623183 2305 10-11 -0.012964500670278767 2305 12-13 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-1.4098848374289048 1213 54-55 -1.2769526329679763 1213 56-57 -1.3737693952034888 1213 58-59 -1.3804929519736575 1213 60-61 -1.41489959526303 1213 62-63 -1.2491985351575394 1213 64-65 -1.340824330886555 1213 66-67 -1.4817794545802343 1213 68-69 -1.5886362181392144 1213 70-71 -1.5137992725520704 1213 72-73 -1.2050933055370834 1213 74-75 -1.1645513228628488 1213 76-77 -1.0220388668801412 1213 78-79 -1.2543501352729258 1213 80-81 -1.4568373614846983 1213 82-83 -1.3823365157431198 1213 84-85 -1.2849280072705191 1213 86-87 -1.5561264593223996 1213 88-89 -1.6953164860066963 1213 90-91 -1.4204017276813161 1213 92-93 -1.5734161021643587 1213 94-95 -1.412247255910053 1213 96-97 -1.680348019534371 1213 98-99 -1.551793117772636 1213 100-101 -1.439010413795696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 13.0 10 8.0 11 18.0 12 36.0 13 79.0 14 298.0 15 805.0 16 1580.0 17 2537.0 18 3167.0 19 3857.0 20 4761.0 21 6006.0 22 7446.0 23 9419.0 24 12307.0 25 15293.0 26 19937.0 27 26736.0 28 38452.0 29 55077.0 30 53666.0 31 54938.0 32 73614.0 33 105570.0 34 164239.0 35 292875.0 36 531555.0 37 643482.0 38 108249.0 39 44.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.437790314256226 21.952237928661546 23.770330958252092 12.83964079883014 2 35.39546686010163 20.733690329355216 31.475725671782495 12.395117138760662 3 41.9210345311811 15.30173975186779 27.748286499593483 15.028939217357626 4 35.98940356831214 14.36283430842987 31.58413791283572 18.06362421042227 5 35.540587800181214 13.420489377326072 34.86395303179781 16.174969790694906 6 29.758334503543278 13.930492212662774 39.61935828370004 16.691815000093914 7 36.89743504887601 17.199313437081017 27.66005117916913 18.243200334873837 8 33.70016806301781 27.54475941228926 21.064136747305312 17.690935777387608 9 34.52715617517551 25.546607300500074 21.331302385528872 18.594934138795548 10-11 30.125631730850287 26.798133916095097 26.597285936012632 16.47894841704198 12-13 28.352338437237538 25.13237534133608 26.33900341045389 20.176282810972484 14-15 27.06932621845688 25.705278824506966 26.000171730172543 21.22522322686361 16-17 27.129388609466165 26.33075118562417 26.738678795747074 19.801181409162595 18-19 24.671700209206705 26.425047382322347 27.81027035874612 21.092982049724828 20-21 25.005456010690203 27.267620902066398 28.360075239281844 19.366847847961555 22-23 24.368690369604476 28.12864199893921 27.3537319560335 20.14893567542282 24-25 25.209475033384525 24.134327877620787 29.391887359317657 21.26430972967703 26-27 22.54242495525624 24.238461655425198 29.538551187666194 23.680562201652368 28-29 23.068057919578404 27.381392141376864 27.07303809458218 22.47751184446255 30-31 20.230261539686218 27.077308988196236 29.02467995130734 23.667749520810208 32-33 20.988960075418166 26.502728452559094 28.22928750761382 24.27902396440892 34-35 21.880794216270896 26.756343506855345 28.80440470004016 22.5584575768336 36-37 20.3702842402684 29.41478471565687 29.506553026610128 20.7083780174646 38-39 20.209268820296387 27.61869452624268 27.161767307007285 25.01026934645365 40-41 23.14279472086267 26.665909339432503 25.436422863202328 24.7548730765025 42-43 23.289915937260524 28.353402949944062 26.909870870347763 21.446810242447647 44-45 20.813197271244942 28.55783309585973 26.59389062261017 24.035079010285155 46-47 22.425526690708985 28.181125564281523 26.640953147442538 22.752394597566948 48-49 21.459278431435692 27.617281614971112 28.866946735489712 22.056493218103483 50-51 21.98624253980578 26.75743492532594 26.728588132876823 24.52773440199146 52-53 20.496421610413957 27.931922491035017 26.235977820335414 25.335678078215608 54-55 22.30769815695824 27.835123713350736 28.533555311828497 21.32362281786253 56-57 22.018202325243834 27.260210767297366 26.451119132346733 24.270467775112067 58-59 20.538532492583194 26.41532138294332 27.319697387209096 25.72644873726439 60-61 20.81453540191657 26.29136784376702 29.753333415343047 23.140763338973365 62-63 20.330908522484457 26.25307797568212 29.58919996448769 23.826813537345732 64-65 23.182965156469482 28.12525788631734 27.520822677759572 21.170954279453607 66-67 21.933800375879564 30.085864526349482 26.04832398554901 21.932011112221947 68-69 21.263458500914567 27.6060922852019 28.424103426418363 22.70634578746517 70-71 20.983259611565014 30.81379795941008 26.71239831942998 21.490544109594925 72-73 23.334855687940266 30.2494395170453 26.011217710724594 20.404487084289837 74-75 24.754671266654977 28.10116723510402 26.473135519520724 20.67102597872028 76-77 23.384180567288986 27.063307747554543 26.51980845670492 23.03270322845155 78-79 23.417399912059757 28.70437093427339 26.24079165900656 21.63743749466029 80-81 22.611882455494577 29.148096183511335 26.390510816686803 21.84951054430729 82-83 22.115244213449266 28.674896596662247 27.037795409757177 22.17206378013131 84-85 22.760739104805506 28.208940137591497 26.566107337825496 22.464213419777504 86-87 20.361302744036365 29.063627002155258 27.913419394434985 22.661650859373392 88-89 19.80401419088811 29.33048209386724 28.40942081011166 22.45608290513299 90-91 21.28193916838787 28.49537987048205 26.836047186490415 23.386633774639666 92-93 21.72190153378652 28.63036111675336 25.86100445523779 23.78673289422233 94-95 22.323874360283902 29.72278092868087 25.77877192852942 22.174572782505813 96-97 21.29288308351101 31.18047422250575 24.513403438072903 23.013239255910335 98-99 21.35763722669864 29.03293709203327 26.417766881266303 23.19165880000179 100-101 22.80570558714017 29.780811591025767 25.943241644119986 21.470241177714076 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 255.0 1 169.5 2 95.5 3 171.0 4 194.5 5 155.0 6 150.0 7 157.5 8 175.5 9 196.0 10 219.0 11 217.5 12 214.5 13 232.0 14 255.0 15 272.5 16 296.5 17 306.0 18 317.0 19 381.5 20 432.5 21 453.0 22 515.0 23 588.5 24 700.5 25 850.5 26 1071.5 27 1423.0 28 1834.5 29 2335.0 30 3099.0 31 4096.0 32 5662.5 33 8360.0 34 13130.5 35 22879.5 36 35525.0 37 49861.0 38 67178.0 39 81943.0 40 92985.5 41 100272.5 42 134313.0 43 172218.0 44 163447.5 45 139696.5 46 134461.0 47 130563.5 48 115196.5 49 103767.0 50 94260.0 51 89002.0 52 88173.5 53 88487.5 54 75136.5 55 48221.0 56 31656.5 57 25305.0 58 22144.5 59 18505.5 60 14534.0 61 11533.0 62 8892.5 63 6590.0 64 5021.0 65 3868.0 66 2939.5 67 2318.5 68 1818.0 69 1301.0 70 943.0 71 738.0 72 504.5 73 305.0 74 202.5 75 141.0 76 105.5 77 76.0 78 50.0 79 36.5 80 28.0 81 19.5 82 10.5 83 8.0 84 6.0 85 5.5 86 4.5 87 4.0 88 3.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17897503919830632 2 0.0 3 0.0 4 0.009793986402905817 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002079545058151235 12-13 0.0 14-15 0.0 16-17 3.354104932501992E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001900659461751129 40-41 0.0059255853807535196 42-43 0.002571480448251527 44-45 0.0012521991748007438 46-47 0.006596406367253918 48-49 0.008362901631704967 50-51 0.0051653215960530685 52-53 0.006439881470403826 54-55 0.018514659227411 56-57 0.02113086107476255 58-59 0.026922282258215996 60-61 0.024976901397364835 62-63 0.009905789900655883 64-65 0.015317079191759099 66-67 0.022785552841463535 68-69 0.026072575675315488 70-71 0.02835336702941684 72-73 0.022092371155413123 74-75 0.031215536571818542 76-77 0.028822941719967122 78-79 0.02043767938871214 80-81 0.00961510080650571 82-83 0.0018112166635510757 84-85 0.007692080645204569 86-87 0.02683283946001594 88-89 0.02446260530771453 90-91 0.020951975478362445 92-93 0.04221700075042508 94-95 0.044385988606776366 96-97 0.03327272093041976 98-99 0.0400703735936238 100-101 0.0397349631003736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2236066.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.05812632378169 #Duplication Level Percentage of deduplicated Percentage of total 1 86.03520415006959 36.18479484435973 2 6.035431003134253 5.076778390965776 3 2.455235574244532 3.097878238086576 4 1.161050913110513 1.9532650388777613 5 0.7544975779386087 1.5866377221964667 6 0.5687474543860191 1.4352271369737875 7 0.36554369844833073 1.0761858132341577 8 0.27123396803994604 0.9126073992899691 9 0.20867439794115145 0.7898808770233233 >10 1.7308467811593558 14.40719250745975 >50 0.22689777500663402 6.547973620777467 >100 0.17183136026864265 14.149979360138481 >500 0.009582120904771346 2.78692376440381 >1k 0.0043727754394771 3.164176209968671 >5k 3.1891871562176954E-4 0.8924198254121406 >10k+ 5.315311926680009E-4 5.9380792508320654 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 55398 2.4774760673432716 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31106 1.3911038404054263 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24743 1.1065415779319572 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10876 0.4863899366118889 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10689 0.4780270349801839 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9175 0.4103188367427437 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5680 0.25401754688815087 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5105 0.22830274240563558 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4579 0.20477928647902166 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2875 0.12857402241257637 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2688 0.12021112078087141 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2638 0.11797505082587008 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2618 0.11708062284386954 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2502 0.11189294054826647 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2361 0.10558722327516272 No Hit CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA 2300 0.10285921793006111 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2248 0.10053370517685972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0025491197487015143 0.0 0.0 2 0.0027280053451016204 0.0 0.0 3 0.0029963337397017798 0.0 0.0 4 0.003175219336101886 0.0 0.0 5 0.003577711928002125 0.0 0.0 6 0.003935483120802338 0.0 0.0 7 0.00442741851090263 0.0 0.0 8 0.004964075300102949 0.0 0.0 9 0.005590174887503321 0.0 0.0 10-11 0.009056083317755379 0.0 0.0 12-13 0.012141859855657213 0.0 2.2360699550013284E-5 14-15 0.015428882689509165 0.0 4.472139910002657E-5 16-17 0.019051316016611315 0.0 4.472139910002657E-5 18-19 0.023657620123914053 0.0 8.944279820005313E-5 20-21 0.027637824643816415 0.0 8.944279820005313E-5 22-23 0.031394422168218646 0.0 8.944279820005313E-5 24-25 0.034994494795770784 0.0 8.944279820005313E-5 26-27 0.039265388409823326 0.0 8.944279820005313E-5 28-29 0.044766120499126594 0.0 8.944279820005313E-5 30-31 0.09532366218170663 0.0 8.944279820005313E-5 32-33 0.10511764858461244 0.0 8.944279820005313E-5 34-35 0.11341346811766737 0.0 8.944279820005313E-5 36-37 0.12112790946242194 0.0 8.944279820005313E-5 38-39 0.12904359710312663 0.0 8.944279820005313E-5 40-41 0.13836800881548217 0.0 8.944279820005313E-5 42-43 0.1499955725814891 0.0 8.944279820005313E-5 44-45 0.16323310671509694 0.0 1.341641973000797E-4 46-47 0.1777899221221556 0.0 1.341641973000797E-4 48-49 0.19433683978916544 0.0 1.341641973000797E-4 50-51 0.2129409418147765 0.0 1.341641973000797E-4 52-53 0.23288668581338834 0.0 1.341641973000797E-4 54-55 0.2550461390674515 0.0 1.341641973000797E-4 56-57 0.28348894889506837 0.0 1.341641973000797E-4 58-59 0.3161802916371878 0.0 1.341641973000797E-4 60-61 0.35202449301585914 0.0 1.341641973000797E-4 62-63 0.39430857586493423 0.0 1.341641973000797E-4 64-65 0.4355640665347087 0.0 1.341641973000797E-4 66-67 0.4811351722176358 0.0 1.341641973000797E-4 68-69 0.5324976990840162 0.0 1.341641973000797E-4 70-71 0.5902553860217006 0.0 1.341641973000797E-4 72-73 0.6561300068960397 0.0 1.341641973000797E-4 74-75 0.7244195833217804 0.0 1.341641973000797E-4 76-77 0.8038447881234274 0.0 1.341641973000797E-4 78-79 0.8951882457852317 0.0 1.341641973000797E-4 80-81 0.9951629334733412 0.0 1.341641973000797E-4 82-83 1.1086211229901086 0.0 1.341641973000797E-4 84-85 1.240839939429337 0.0 1.341641973000797E-4 86-87 1.3818912321908208 0.0 1.341641973000797E-4 88-89 1.5348831385120119 0.0 1.341641973000797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 10915 0.0 56.77957 4 TCGGAAG 11085 0.0 55.951645 3 GAAGAGC 11070 0.0 55.85586 6 GATCGGA 11555 0.0 53.63767 1 ATCGGAA 11555 0.0 53.429214 2 GGAAGAG 12880 0.0 48.264652 5 TTGTGTT 23125 0.0 44.12056 1 GCCGTCT 7025 0.0 43.74823 50-51 TATGCCG 6955 0.0 43.60557 48-49 TCGTATG 7125 0.0 43.460754 44-45 ATGCCGT 7065 0.0 43.2628 48-49 TGCCGTC 7070 0.0 43.13384 50-51 CGTATGC 7115 0.0 43.054577 46-47 TCTCGTA 7025 0.0 42.89438 42-43 GTATGCC 7170 0.0 42.889908 46-47 TGTGTTT 43250 0.0 42.605824 1 TCGATCT 7010 0.0 42.5458 38-39 CTCGTAT 7050 0.0 42.474716 44-45 ACTCGAT 7250 0.0 42.054466 36-37 CGTCTTC 7365 0.0 41.763203 52-53 >>END_MODULE