##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4562153 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.506248256031746 33.0 33.0 33.0 22.0 33.0 2 29.82911270183179 33.0 33.0 33.0 15.0 33.0 3 29.539569365604354 33.0 33.0 33.0 15.0 33.0 4 32.75120562594021 37.0 37.0 37.0 15.0 37.0 5 32.95620949143968 37.0 37.0 37.0 15.0 37.0 6 33.78922692860147 37.0 37.0 37.0 22.0 37.0 7 32.65132953673408 37.0 37.0 37.0 15.0 37.0 8 33.38846044838917 37.0 37.0 37.0 22.0 37.0 9 32.427249371075455 37.0 37.0 37.0 15.0 37.0 10-11 32.512135936694804 37.0 33.0 37.0 15.0 37.0 12-13 32.48411966893701 37.0 33.0 37.0 15.0 37.0 14-15 33.82199829773354 37.0 33.0 40.0 15.0 40.0 16-17 33.718268545574865 37.0 33.0 40.0 10.5 40.0 18-19 33.7756664452069 37.0 33.0 40.0 10.5 40.0 20-21 33.88618016537367 37.0 33.0 40.0 15.0 40.0 22-23 34.987530120098995 37.0 33.0 40.0 22.0 40.0 24-25 34.01449841774267 37.0 33.0 40.0 15.0 40.0 26-27 33.95505477348085 37.0 33.0 40.0 18.5 40.0 28-29 33.457391499145245 37.0 33.0 40.0 15.0 40.0 30-31 33.44342199834158 37.0 33.0 40.0 10.5 40.0 32-33 33.171089614925236 37.0 33.0 40.0 6.0 40.0 34-35 33.4558717123253 37.0 33.0 40.0 10.5 40.0 36-37 33.24503989673296 37.0 33.0 40.0 10.5 40.0 38-39 33.53980565754809 37.0 33.0 40.0 15.0 40.0 40-41 32.88879165166096 37.0 33.0 40.0 6.0 40.0 42-43 32.77194397031401 37.0 33.0 40.0 6.0 40.0 44-45 32.63636269980424 37.0 33.0 40.0 6.0 40.0 46-47 32.6406179275443 37.0 33.0 40.0 6.0 40.0 48-49 32.57358576093349 37.0 33.0 40.0 6.0 40.0 50-51 31.673876676209677 37.0 30.0 38.5 10.5 38.5 52-53 32.15582850903948 37.0 33.0 38.5 10.5 40.0 54-55 32.69405651235283 37.0 33.0 40.0 10.5 40.0 56-57 33.01522132203809 37.0 33.0 40.0 10.5 40.0 58-59 32.30858653797889 37.0 33.0 40.0 6.0 40.0 60-61 32.261032893899 37.0 33.0 40.0 6.0 40.0 62-63 31.747900169064913 37.0 33.0 40.0 6.0 40.0 64-65 31.627746811647924 37.0 33.0 37.0 6.0 40.0 66-67 31.59950323893127 37.0 33.0 37.0 6.0 40.0 68-69 31.891996169352495 37.0 33.0 37.0 15.0 40.0 70-71 31.36542658696453 37.0 33.0 37.0 10.5 40.0 72-73 31.95984220608121 37.0 33.0 37.0 15.0 37.0 74-75 32.14406958731985 37.0 30.0 37.0 22.0 37.0 76-77 31.73199221946305 37.0 27.0 37.0 22.0 37.0 78-79 31.059365830124506 35.0 27.0 37.0 15.0 37.0 80-81 30.419846725877015 33.0 27.0 37.0 15.0 37.0 82-83 30.00095842905751 33.0 27.0 37.0 6.0 37.0 84-85 29.776071407513076 33.0 27.0 37.0 6.0 37.0 86-87 29.594343284848186 33.0 27.0 37.0 6.0 37.0 88-89 29.452480440704203 33.0 27.0 37.0 6.0 37.0 90-91 29.314884222427438 33.0 27.0 37.0 6.0 37.0 92-93 29.18020997980559 33.0 27.0 37.0 6.0 37.0 94-95 29.04008184293688 33.0 27.0 37.0 6.0 37.0 96-97 28.876792711686786 33.0 27.0 37.0 6.0 37.0 98-99 28.743629816886894 33.0 27.0 37.0 6.0 37.0 100-101 27.649713194625434 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.23254149410947633 2306 2 -0.39917498255056927 2306 3 -0.3191145989115718 2306 4 -0.28512636068034425 2306 5 -0.31225710847483157 2306 6 -0.5106090662281275 2306 7 0.2678961118071541 2306 8 -0.5083857025437979 2306 9 -0.0868548277164578 2306 10-11 -0.2311773845103673 2306 12-13 -0.2815095502073177 2306 14-15 0.02229049763757729 2306 16-17 -0.015749824274131186 2306 18-19 0.025888415287134592 2306 20-21 -0.08208015833948679 2306 22-23 -0.1194910385491923 2306 24-25 -0.004147074018000296 2306 26-27 -0.18602391799127815 2306 28-29 -0.11456002087663819 2306 30-31 0.01981589717856025 2306 32-33 0.006426211880700805 2306 34-35 -0.34968307837096546 2306 36-37 -0.23917016115731116 2306 38-39 -0.1839555549797396 2306 40-41 0.07930300226458797 2306 42-43 -0.05922824050389153 2306 44-45 0.07013769813244863 2306 46-47 -0.04288509869282109 2306 48-49 -0.01049391718660786 2306 50-51 0.06629340007356532 2306 52-53 -0.1293218178427793 2306 54-55 0.13653560618799077 2306 56-57 0.16336625555938156 2306 58-59 0.3482394817828194 2306 60-61 0.11141262012112207 2306 62-63 0.18950278491810835 2306 64-65 0.12912479659168596 2306 66-67 0.029791134391668805 2306 68-69 -0.02403922023271221 2306 70-71 0.17060717272497783 2306 72-73 -0.15644591902126592 2306 74-75 0.05604972168390532 2306 76-77 0.2315362650268895 2306 78-79 0.2083968634485167 2306 80-81 0.2894501607486042 2306 82-83 0.3083690609157088 2306 84-85 0.1615951626735992 2306 86-87 0.18184661130765534 2306 88-89 0.2006741759399695 2306 90-91 0.23068405300199046 2306 92-93 0.10872268225275761 2306 94-95 0.17295846360683242 2306 96-97 0.2567203355751033 2306 98-99 0.1695946241931061 2306 100-101 0.15695371444632045 2307 1 -0.19716474764940628 2307 2 -0.5007630154206879 2307 3 -0.43550546748751273 2307 4 -0.387160998413286 2307 5 -0.44577456837441076 2307 6 -0.40355835072012525 2307 7 0.15123445965122784 2307 8 -0.49032406167052045 2307 9 -0.3447287065031972 2307 10-11 -0.4324144799803804 2307 12-13 -0.3575442631579264 2307 14-15 -0.08572159360144127 2307 16-17 -0.03359779997882839 2307 18-19 0.007594539885189988 2307 20-21 -0.2031874093220125 2307 22-23 -0.22438159685228243 2307 24-25 -0.16524003364492756 2307 26-27 -0.37694679168201617 2307 28-29 -0.2746398926305247 2307 30-31 -0.04083962473798408 2307 32-33 -0.1714576802571699 2307 34-35 -0.33218721534233 2307 36-37 -0.3523445345001761 2307 38-39 -0.4158015374164066 2307 40-41 -0.039862927895818245 2307 42-43 -0.31806960645661064 2307 44-45 -0.17446660995908303 2307 46-47 -0.40047150498102724 2307 48-49 -0.3580930634661499 2307 50-51 -0.3863462697209066 2307 52-53 -0.4296485445582121 2307 54-55 -0.25651945313993707 2307 56-57 -0.1094717067941744 2307 58-59 -0.06866451227499226 2307 60-61 -0.14588043728875277 2307 62-63 -0.11727597173394955 2307 64-65 -0.22711662389384557 2307 66-67 -0.13929064601036956 2307 68-69 -0.19751018621552774 2307 70-71 -0.31440265258200384 2307 72-73 -0.4796268268984356 2307 74-75 -0.22129448373552307 2307 76-77 -0.03283947539696186 2307 78-79 -0.05580916712746031 2307 80-81 0.0011711878457667524 2307 82-83 -0.039238547350713304 2307 84-85 -0.14019501766074427 2307 86-87 -0.13296139561236942 2307 88-89 -0.09924214441205237 2307 90-91 -0.029358209921344525 2307 92-93 0.050981139538532005 2307 94-95 -0.1183080607730922 2307 96-97 -0.031592955385615795 2307 98-99 -0.12148197559252338 2307 100-101 -0.293063679637811 2201 1 -0.12791686157909155 2201 2 -0.38162638880742605 2201 3 -0.31910582351777705 2201 4 -0.3751004732686454 2201 5 -0.3377638874665365 2201 6 -0.29294419851719056 2201 7 0.015052884217318763 2201 8 -0.34824573501276035 2201 9 -0.35694499488771214 2201 10-11 -0.272501262356883 2201 12-13 -0.27146282123536025 2201 14-15 -0.10377900348128577 2201 16-17 -0.008097132422079767 2201 18-19 0.014870908376515501 2201 20-21 -0.210141125387878 2201 22-23 -0.07652890437342563 2201 24-25 -0.07627697178466519 2201 26-27 -0.16429659141499542 2201 28-29 -0.2393038890702215 2201 30-31 -0.021836509473182275 2201 32-33 -0.09625741323447556 2201 34-35 -0.2815975402199413 2201 36-37 -0.3608113791819747 2201 38-39 -0.24285983737190975 2201 40-41 -0.052991543828170506 2201 42-43 -0.15793386991901315 2201 44-45 -0.2797180563320296 2201 46-47 -0.22589429091782165 2201 48-49 -0.2322919953753697 2201 50-51 -0.2887272221018584 2201 52-53 -0.3188341253325788 2201 54-55 -0.07063827824007518 2201 56-57 -0.006122797136853819 2201 58-59 -0.0918064793421891 2201 60-61 -0.16354843906890437 2201 62-63 -0.01709795660266522 2201 64-65 0.01357095955074783 2201 66-67 -0.006524735908012502 2201 68-69 -0.1300201995666619 2201 70-71 -0.027497890677238956 2201 72-73 -0.21162504674712324 2201 74-75 0.02236372721240798 2201 76-77 0.15026021307655668 2201 78-79 0.0615806860100534 2201 80-81 0.09744399603446396 2201 82-83 0.116680455764552 2201 84-85 0.017437381093149185 2201 86-87 -0.03159290429060846 2201 88-89 0.02924027659373607 2201 90-91 0.16989635171639605 2201 92-93 0.11827085916469926 2201 94-95 0.19226158764702816 2201 96-97 0.034172509678917606 2201 98-99 -0.011468624457677379 2201 100-101 -0.046190070381026516 1101 1 -0.12228074931282507 1101 2 -0.34065336101809507 1101 3 -0.20170192615371363 1101 4 -0.2244030041477103 1101 5 -0.2926633162130585 1101 6 -0.15245852362826184 1101 7 0.12648287830779736 1101 8 -0.2523865931332381 1101 9 -0.1863262463924471 1101 10-11 -0.13262541696740016 1101 12-13 -0.1772355455234802 1101 14-15 0.07623341745154733 1101 16-17 0.07532201660722393 1101 18-19 0.032749664751129615 1101 20-21 0.05530068409215261 1101 22-23 0.0450828803741814 1101 24-25 -0.0680872725864603 1101 26-27 -0.09875893464860042 1101 28-29 -0.1351478923678826 1101 30-31 0.09228596347800533 1101 32-33 0.08608242786512932 1101 34-35 -0.11982483052322834 1101 36-37 -0.1917998738597575 1101 38-39 -0.11565270509353098 1101 40-41 -0.045888721850595005 1101 42-43 -0.17402645602653166 1101 44-45 -0.1676022662439749 1101 46-47 -0.10829641907412935 1101 48-49 -0.1570449446629425 1101 50-51 -0.19405846521653913 1101 52-53 -0.23123782206483057 1101 54-55 0.08548710204839693 1101 56-57 0.053998986370480395 1101 58-59 -0.12369416930403077 1101 60-61 -0.10363421828636632 1101 62-63 0.1348675967398627 1101 64-65 0.0026528501589915265 1101 66-67 -0.07226959061410554 1101 68-69 -0.09811399676073407 1101 70-71 0.091268023072967 1101 72-73 0.002219186365149284 1101 74-75 0.056380394243859655 1101 76-77 0.05650369254280463 1101 78-79 -0.029644499483172382 1101 80-81 0.08059539279663142 1101 82-83 0.19788773030911955 1101 84-85 0.15513591538659455 1101 86-87 0.08799167843170963 1101 88-89 0.12426077751663911 1101 90-91 -0.01124662945339594 1101 92-93 0.028788981688720128 1101 94-95 0.10594449454657351 1101 96-97 0.0620086226747496 1101 98-99 0.08798447147350785 1101 100-101 0.04503465913082039 2304 1 -0.028379805226176558 2304 2 -0.2897394631843717 2304 3 -0.21325600987736593 2304 4 -0.06423303195801111 2304 5 -0.14654486627161134 2304 6 -0.03334076894442717 2304 7 0.10659942543895085 2304 8 -0.04721241280537214 2304 9 0.013514576207086293 2304 10-11 -0.03218813652244279 2304 12-13 -0.03965687163589138 2304 14-15 -0.003485643584745901 2304 16-17 0.04134587492103492 2304 18-19 0.11624461498532668 2304 20-21 0.08408755104180443 2304 22-23 0.09905225244678206 2304 24-25 0.008594610463887875 2304 26-27 0.04526459433266439 2304 28-29 -0.03680324585149464 2304 30-31 0.2416268409662905 2304 32-33 0.03148869616138228 2304 34-35 -0.1307637027421933 2304 36-37 -0.12798940474383613 2304 38-39 -0.2233261535712856 2304 40-41 0.04807854615291518 2304 42-43 -0.006135465101472448 2304 44-45 0.05893965047148697 2304 46-47 -0.08766510686686502 2304 48-49 0.03110685934759516 2304 50-51 -0.022256650228246855 2304 52-53 -0.018354058159680164 2304 54-55 0.15771384751092654 2304 56-57 0.24786150832598253 2304 58-59 0.10478028912687876 2304 60-61 0.16626753083541246 2304 62-63 0.2751769863768665 2304 64-65 0.13146304483112203 2304 66-67 0.11646311942687859 2304 68-69 0.1869073183739296 2304 70-71 0.11865522667367046 2304 72-73 0.04835318812159528 2304 74-75 0.1421966815682154 2304 76-77 0.19932424289409312 2304 78-79 0.18789337376038517 2304 80-81 0.21733701311278963 2304 82-83 0.15385093654448312 2304 84-85 0.1859742496957324 2304 86-87 0.20297219292233848 2304 88-89 0.3351273795365479 2304 90-91 0.21244335883700316 2304 92-93 0.23699347890768863 2304 94-95 0.2977258177315818 2304 96-97 0.2928044181958285 2304 98-99 0.1818808414969304 2304 100-101 0.2132481215137254 2202 1 0.22432563458863797 2202 2 0.10384312610134216 2202 3 0.08699613959353059 2202 4 0.07760931261870496 2202 5 0.006937689945324621 2202 6 0.2704662747063722 2202 7 0.3448951430315432 2202 8 0.2607736983057407 2202 9 0.2451986039848606 2202 10-11 0.24145190977385766 2202 12-13 0.1680936243958584 2202 14-15 0.32013683492053957 2202 16-17 0.3651140627517293 2202 18-19 0.2845440528160097 2202 20-21 0.30354749647830914 2202 22-23 0.19636334702349956 2202 24-25 0.15403628539775127 2202 26-27 0.28538322859192533 2202 28-29 0.1122759952199388 2202 30-31 0.20434902185253634 2202 32-33 0.14105900866138654 2202 34-35 0.18722261504880322 2202 36-37 0.10857743553394528 2202 38-39 0.10503859510595959 2202 40-41 0.11866696381164132 2202 42-43 0.015386112279479391 2202 44-45 0.030305184532330998 2202 46-47 0.18064776515694092 2202 48-49 0.1834475091491825 2202 50-51 0.034208296861695686 2202 52-53 0.033959723255662766 2202 54-55 0.3298957671537863 2202 56-57 0.3351692960243966 2202 58-59 0.21881385394169683 2202 60-61 0.18224595345446204 2202 62-63 0.34936473439273996 2202 64-65 0.2726274793549308 2202 66-67 0.29734832941365497 2202 68-69 0.285912154683448 2202 70-71 0.2646818057080651 2202 72-73 0.2977207476453998 2202 74-75 0.361863306898897 2202 76-77 0.38932093601578543 2202 78-79 0.17630896569160726 2202 80-81 0.21316001245141436 2202 82-83 0.38608163264236595 2202 84-85 0.43537487800260877 2202 86-87 0.3535415960308086 2202 88-89 0.48696786399422365 2202 90-91 0.4757535440221865 2202 92-93 0.3985272504023989 2202 94-95 0.3545545214352863 2202 96-97 0.3402818900873612 2202 98-99 0.27926840763449334 2202 100-101 0.2980726502835651 1102 1 -0.009059512987253271 1102 2 -0.1585504889770064 1102 3 -0.05485705158208276 1102 4 -0.03251238997295047 1102 5 0.03551182422132371 1102 6 0.12571967070382328 1102 7 0.11588877245012696 1102 8 0.22285176058835532 1102 9 0.012270517553837124 1102 10-11 0.09854075428918918 1102 12-13 0.16136311662980773 1102 14-15 0.09573124439375391 1102 16-17 0.17191025149627137 1102 18-19 0.1488374107171424 1102 20-21 0.19853406265511353 1102 22-23 0.11776628190124683 1102 24-25 0.0341865264083836 1102 26-27 0.10775910101254027 1102 28-29 0.007529310046258786 1102 30-31 0.12607217686640837 1102 32-33 0.0062706826943284 1102 34-35 -0.03644878254230122 1102 36-37 -0.019152857128588607 1102 38-39 0.007115629220841413 1102 40-41 0.11145815511984125 1102 42-43 0.17126574360017344 1102 44-45 0.012153221340739151 1102 46-47 0.11070827823969864 1102 48-49 0.0565122877751989 1102 50-51 0.03629557883263956 1102 52-53 0.05878638905650746 1102 54-55 0.28264625113668984 1102 56-57 0.33392392785241043 1102 58-59 0.18785683947023557 1102 60-61 0.18421049790175203 1102 62-63 0.334756466300707 1102 64-65 0.2701479040423216 1102 66-67 0.2651006262186222 1102 68-69 0.11856060654693934 1102 70-71 0.051269560500877276 1102 72-73 0.1900926900301272 1102 74-75 0.06415754144830998 1102 76-77 0.0828093535709371 1102 78-79 -0.13331867524516383 1102 80-81 -0.017274056968847873 1102 82-83 0.15013506219055373 1102 84-85 0.1526591893805005 1102 86-87 0.1238674913822706 1102 88-89 0.22214665695235425 1102 90-91 0.2950246542674755 1102 92-93 0.3615537636015027 1102 94-95 0.22682517576935268 1102 96-97 0.15529131270353247 1102 98-99 0.09256887342153775 1102 100-101 0.08482724558456667 2305 1 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1213 60-61 -0.41870706072993613 1213 62-63 -0.3977012905988211 1213 64-65 -0.15152398974334602 1213 66-67 -0.1286623792433339 1213 68-69 -0.09093688280388434 1213 70-71 -0.32064057974730176 1213 72-73 -0.29886613178722143 1213 74-75 -0.7578062867068631 1213 76-77 -0.6380237814220173 1213 78-79 -0.6721002147654467 1213 80-81 -1.0003123504890326 1213 82-83 -1.3537947463962468 1213 84-85 -1.3088039607905912 1213 86-87 -1.2249278118268982 1213 88-89 -1.3071022699695334 1213 90-91 -1.2089567171508406 1213 92-93 -1.1478344798689086 1213 94-95 -1.2309690910830078 1213 96-97 -1.2833193707479431 1213 98-99 -1.3357863317553509 1213 100-101 -1.1858073733570968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1822.0 3 534.0 4 822.0 5 687.0 6 1219.0 7 6568.0 8 35488.0 9 110533.0 10 152223.0 11 115836.0 12 72196.0 13 48296.0 14 34346.0 15 26573.0 16 22806.0 17 20680.0 18 19669.0 19 19594.0 20 19944.0 21 20604.0 22 21133.0 23 22075.0 24 24114.0 25 26836.0 26 31506.0 27 38029.0 28 46934.0 29 56356.0 30 66203.0 31 85332.0 32 115397.0 33 164578.0 34 249865.0 35 423757.0 36 829022.0 37 1313121.0 38 317356.0 39 99.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.916007242231565 22.294851932817213 22.444988795182972 13.34415202976825 2 29.49956836434573 29.09819442117231 29.689725226737735 11.712511987744227 3 34.739900484580026 20.27077819091294 30.402017883268726 14.587303441238305 4 26.29006896848491 17.51490833694488 33.57323230659998 22.62179038797023 5 37.770700497092975 15.9047136006745 29.226893081098172 17.097692821134356 6 33.700924184304654 16.29935026284352 34.88960967248356 15.11011588036827 7 35.59483227682582 20.73307376632692 27.214808269182356 16.457285687664903 8 25.757203691573043 34.2430815922789 23.98646876745306 16.01324594869499 9 35.26983766114068 26.266566130218692 21.952130489053058 16.51146571958758 10-11 27.693151538487697 31.468839165534014 25.555263022213154 15.282746273765135 12-13 30.16240919892235 23.725711932689443 25.64000300274066 20.471875865647547 14-15 25.34787490581331 24.094334709782107 28.616809982587466 21.940980401817118 16-17 28.56728907085485 25.054581924783754 29.374297325263505 17.003831679097893 18-19 28.46544732061438 25.39462592265908 29.602147726176764 16.537779030549775 20-21 26.44242927256879 29.20827735718333 27.627015347110646 16.722278023137235 22-23 31.31679016625817 25.16766248954989 26.781904914457243 16.733642429734694 24-25 24.376293172927774 31.963997713357568 26.665952457920405 16.99375665579425 26-27 22.767586215995145 33.50124673474234 26.52568889240701 17.205478156855502 28-29 24.489977138167795 31.392738592658752 26.300107844644295 17.817176424529162 30-31 28.598107467112865 26.929039961469364 26.768902225214557 17.703950346203214 32-33 23.075296289627932 26.42552183049624 29.393672193126914 21.105509686748917 34-35 18.902172488612486 26.295194948772682 36.483545164385646 18.319087398229186 36-37 22.017724008971044 26.958623461097268 28.84300850894884 22.180644020982847 38-39 19.603345320180793 26.348597318905124 26.236547787931773 27.811509572982306 40-41 22.920742044772133 25.79249872742086 28.775529795946376 22.51122943186063 42-43 25.544341204648134 25.97578989555483 30.914446138276787 17.565422761520253 44-45 21.59649379192374 29.467714077823047 31.45666486438757 17.47912726586564 46-47 24.54973338329727 30.64397714402867 27.420907267806005 17.38538220486805 48-49 19.737481165381695 28.795057356224497 31.13504833760435 20.33241314078946 50-51 18.964838840791103 26.530837858485256 32.06745680136797 22.43686649935567 52-53 27.639728051816746 26.06736538208561 26.600839092045387 19.692067474052248 54-55 29.303434531106426 26.192879691783787 26.77601937831569 17.72766639879409 56-57 29.46192249816937 26.080172608535275 26.368212771826215 18.089692121469145 58-59 26.447688454772205 26.06022322387392 26.488323201406587 21.003765119947285 60-61 20.830113472141097 26.415328946956144 25.76007688748181 26.994480693420954 62-63 25.265985577636695 26.672367208419708 26.97841307384837 21.08323414009523 64-65 20.451262902807283 29.215166268642275 30.927528774304776 19.406042054245667 66-67 19.403601859628775 28.734888976713734 28.248934610591014 23.612574553066477 68-69 19.112411501291692 26.39158144080163 36.00689078276442 18.489116275142255 70-71 18.612533874221512 33.646333888513276 30.085134919718783 17.655997317546436 72-73 18.35567868205257 38.613061572738374 25.562656837065546 17.468602908143506 74-75 19.015452292076066 38.299882901870184 25.015621145556537 17.66904366049721 76-77 19.368678610862116 37.2647136575256 25.403401012466496 17.963206719145795 78-79 19.037766650360297 36.45885023607592 26.390308520169516 18.11307459339427 80-81 18.319855793956688 36.4509776630591 26.267877061621565 18.96128948136265 82-83 18.01418308689926 35.36880765583619 26.66908377592935 19.9479254813352 84-85 18.34186128091862 34.36468165078192 27.052024329118296 20.241432739181164 86-87 18.590568279231505 33.77973225521127 27.848686412591935 19.7810130529653 88-89 19.0509187801857 33.93366104741744 27.434802408730413 19.58061776366645 90-91 19.479401722249428 33.06228026123052 27.089564293613495 20.36875372290656 92-93 19.085491143064058 33.67223251604882 26.776711233994465 20.46556510689265 94-95 18.795223425627288 33.939932890849455 26.68584751522225 20.578996168301007 96-97 19.41054124621852 34.101261932498055 26.42245225516876 20.065744566114663 98-99 20.05736131150396 32.86904739784564 26.25364074240745 20.81995054824295 100-101 20.128994889439785 33.03857719259262 25.6500953426624 21.182332575305203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 880.0 1 798.0 2 549.0 3 461.0 4 502.0 5 461.0 6 552.5 7 779.5 8 1028.5 9 1289.0 10 1557.5 11 1757.5 12 1802.5 13 1704.5 14 1585.0 15 1512.5 16 1408.5 17 1310.0 18 1332.0 19 1406.0 20 1559.5 21 1901.0 22 2346.0 23 2996.5 24 3962.5 25 5236.0 26 6998.5 27 9304.0 28 12038.5 29 15331.5 30 19300.0 31 24193.5 32 31581.5 33 46124.0 34 70120.0 35 110337.5 36 161643.5 37 219845.5 38 285717.0 39 321096.5 40 319147.0 41 290253.0 42 260684.5 43 265377.5 44 263272.0 45 247847.5 46 248645.5 47 231451.0 48 186982.0 49 149923.0 50 126179.0 51 110761.0 52 97278.0 53 82968.5 54 69500.5 55 55738.5 56 43500.5 57 33998.0 58 26517.0 59 20340.5 60 15425.5 61 11607.5 62 8492.0 63 6177.5 64 4494.0 65 3147.0 66 2227.0 67 1601.5 68 1142.5 69 838.0 70 622.5 71 463.0 72 350.5 73 261.0 74 193.0 75 151.5 76 128.0 77 104.0 78 86.5 79 64.5 80 54.0 81 52.0 82 40.0 83 31.5 84 31.0 85 27.0 86 20.0 87 17.0 88 15.5 89 11.0 90 9.5 91 7.0 92 8.0 93 6.5 94 2.5 95 3.5 96 2.0 97 2.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18210700079545777 2 0.18727999696634462 3 0.15959569966197976 4 0.16042863972339375 5 0.16818813397972407 6 0.16531668271537583 7 0.13379647723344656 8 0.11591018538834624 9 0.1154060374564378 10-11 0.09355232058197084 12-13 0.13842148652182423 14-15 0.11789389790302957 16-17 0.10987136994309486 18-19 0.11283049910864454 20-21 0.10478605167340946 22-23 0.10484085036166037 24-25 0.46024322288182795 26-27 0.3128566709621532 28-29 0.3971041742791177 30-31 0.26319809966916935 32-33 0.29174821624789876 34-35 0.31101543503692225 36-37 0.2914194241183932 38-39 0.3398176255816059 40-41 0.33502822022847545 42-43 0.3777273581135924 44-45 0.33169645998281955 46-47 0.35525989593071516 48-49 0.35077736323178993 50-51 0.3666141841363058 52-53 0.35700249421709446 54-55 0.3335048166950999 56-57 0.3487826909794564 58-59 0.34088072013367376 60-61 0.31144286480527944 62-63 0.3207257625949853 64-65 0.32206285058830775 66-67 0.3337020919728032 68-69 0.32066000416908425 70-71 0.3085823732785814 72-73 0.29467446620049786 74-75 0.30316826287939047 76-77 0.2928551497505673 78-79 0.3031025044534894 80-81 0.3116072608700322 82-83 0.3375489598880178 84-85 0.3362009121570452 86-87 0.32850717632661597 88-89 0.34213013022579475 90-91 0.3317402989334202 92-93 0.31541028983464603 94-95 0.3077494332171674 96-97 0.29231812260570833 98-99 0.31758031788938246 100-101 0.32021065492542666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4562153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.5130677421814 #Duplication Level Percentage of deduplicated Percentage of total 1 91.3787255681046 43.41683578111557 2 4.01652784946926 3.8167511960038225 3 1.2493533080691648 1.7808182508062593 4 0.7276415210560591 1.382899235278418 5 0.41882257117647215 0.9949772598131155 6 0.30760304677014577 0.8769098639334796 7 0.20527867232797511 0.6827393625040897 8 0.148408570395691 0.564107716698461 9 0.1523696668138302 0.6515595271061212 >10 1.0592049532063597 10.259675087402236 >50 0.16296172193023314 5.408998232935144 >100 0.13958940068733677 13.612452336907346 >500 0.022985748751726445 7.57969450717832 >1k 0.010248643887173205 7.671005640681952 >5k 2.3229790399316934E-4 0.8064204998883844 >10k+ 4.6459449922988647E-5 0.4941555017472157 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 22386 0.4906893740740392 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9181 0.20124270273267908 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8610 0.1887266823361689 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7097 0.15556251620671205 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5981 0.13110038177150132 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5663 0.12412998862598426 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4691 0.10282425863402651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00561138567689422 0.0 2.1919475300368048E-5 2 0.006027855707601214 0.0 2.1919475300368048E-5 3 0.006816956818414464 0.0 2.1919475300368048E-5 4 0.00707999052201888 0.0 2.1919475300368048E-5 5 0.007847172157531761 0.0 2.1919475300368048E-5 6 0.008438997990641699 0.0 2.1919475300368048E-5 7 0.009381535428557526 0.0 2.1919475300368048E-5 8 0.010433670242975191 0.0 2.1919475300368048E-5 9 0.01135428820559065 0.0 2.1919475300368048E-5 10-11 0.016340968836424382 0.0 4.38389506007361E-5 12-13 0.021064615763653698 0.0 6.575842590110415E-5 14-15 0.026095135345088165 0.0 6.575842590110415E-5 16-17 0.02978856693320018 0.0 6.575842590110415E-5 18-19 0.03424918015682508 0.0 6.575842590110415E-5 20-21 0.03897282708405439 0.0 6.575842590110415E-5 22-23 0.043860870076036465 0.0 6.575842590110415E-5 24-25 0.04897906755867241 0.0 7.671816355128817E-5 26-27 0.055094601167475096 0.0 8.767790120147219E-5 28-29 0.06267873962140244 0.0 8.767790120147219E-5 30-31 0.100292559236834 0.0 8.767790120147219E-5 32-33 0.10941106096178713 0.0 1.0959737650184024E-4 34-35 0.11681984361331152 0.0 1.0959737650184024E-4 36-37 0.12453549891904109 0.0 1.0959737650184024E-4 38-39 0.13407047067470118 0.0 1.315168518022083E-4 40-41 0.1439890332481177 0.0 1.315168518022083E-4 42-43 0.15394047503448482 2.1919475300368048E-5 1.5343632710257635E-4 44-45 0.16458238029281352 2.1919475300368048E-5 1.5343632710257635E-4 46-47 0.17566267505714955 2.1919475300368048E-5 1.6439606475276037E-4 48-49 0.18847460837021468 2.1919475300368048E-5 1.7535580240294439E-4 50-51 0.20175781040223773 2.1919475300368048E-5 1.863155400531284E-4 52-53 0.21533692535081572 2.1919475300368048E-5 1.9727527770331245E-4 54-55 0.23109702809178034 2.1919475300368048E-5 1.9727527770331245E-4 56-57 0.24815037987546668 2.1919475300368048E-5 2.0823501535349647E-4 58-59 0.2658065172299131 2.1919475300368048E-5 2.191947530036805E-4 60-61 0.28530389050959054 2.1919475300368048E-5 2.191947530036805E-4 62-63 0.30594107650488706 2.1919475300368048E-5 2.191947530036805E-4 64-65 0.32908804242207573 2.1919475300368048E-5 2.191947530036805E-4 66-67 0.3531227470889293 2.1919475300368048E-5 2.3015449065386454E-4 68-69 0.37942611744937094 2.1919475300368048E-5 2.4111422830404855E-4 70-71 0.4078666366515985 2.1919475300368048E-5 2.4111422830404855E-4 72-73 0.44039513799734464 2.1919475300368048E-5 2.4111422830404855E-4 74-75 0.47580605034508927 2.1919475300368048E-5 2.520739659542326E-4 76-77 0.5157214148670595 3.287921295055207E-5 2.630337036044166E-4 78-79 0.5595603654677956 4.3838950600736096E-5 2.630337036044166E-4 80-81 0.6050323169784091 4.3838950600736096E-5 2.630337036044166E-4 82-83 0.6567622786872778 4.3838950600736096E-5 2.630337036044166E-4 84-85 0.7145858545296486 4.3838950600736096E-5 2.630337036044166E-4 86-87 0.7777468226076591 4.3838950600736096E-5 2.630337036044166E-4 88-89 0.848130257797141 4.3838950600736096E-5 2.630337036044166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 10410 0.0 90.52699 2 GAGCGGC 6850 0.0 90.04713 9 CGGGAGA 9090 0.0 87.94963 4 AGAGCGG 7335 0.0 87.19567 8 GAGAGCG 8130 0.0 85.084 7 TCGGGAG 9535 0.0 83.79528 3 AAGCGGC 2115 0.0 82.04533 9 ATTCGGA 885 0.0 81.45753 2 ATCCGGA 1285 0.0 80.46079 2 GATCGGG 12190 0.0 79.41433 1 TCGGAGA 1340 0.0 78.55765 4 GAACCGG 810 0.0 77.880035 1 CGGAAAG 2575 0.0 77.34146 5 AATCGGA 1525 0.0 76.81712 2 GATCCGG 1440 0.0 74.769226 1 TCGGGAA 3045 0.0 74.43564 3 AGAGCGT 19990 0.0 73.358475 8 GGATCGG 1690 0.0 73.25096 1 GAGCGTC 19940 0.0 72.876656 9 CGGAGAG 2760 0.0 72.50095 5 >>END_MODULE