FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562153
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT223860.4906893740740392Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC91810.20124270273267908No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG86100.1887266823361689No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA70970.15556251620671205No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC59810.13110038177150132No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG56630.12412998862598426No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA46910.10282425863402651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA104100.090.526992
GAGCGGC68500.090.047139
CGGGAGA90900.087.949634
AGAGCGG73350.087.195678
GAGAGCG81300.085.0847
TCGGGAG95350.083.795283
AAGCGGC21150.082.045339
ATTCGGA8850.081.457532
ATCCGGA12850.080.460792
GATCGGG121900.079.414331
TCGGAGA13400.078.557654
GAACCGG8100.077.8800351
CGGAAAG25750.077.341465
AATCGGA15250.076.817122
GATCCGG14400.074.7692261
TCGGGAA30450.074.435643
AGAGCGT199900.073.3584758
GGATCGG16900.073.250961
GAGCGTC199400.072.8766569
CGGAGAG27600.072.500955