##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5145300 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.074876294871046 33.0 33.0 33.0 27.0 33.0 2 30.570413581326648 33.0 33.0 33.0 22.0 33.0 3 30.419913318951277 33.0 33.0 33.0 22.0 33.0 4 33.75730958350339 37.0 37.0 37.0 22.0 37.0 5 33.87425903251511 37.0 37.0 37.0 22.0 37.0 6 34.47253571220337 37.0 37.0 37.0 27.0 37.0 7 33.77446387965716 37.0 37.0 37.0 22.0 37.0 8 34.16991603988106 37.0 37.0 37.0 27.0 37.0 9 33.56142440674013 37.0 37.0 37.0 22.0 37.0 10-11 33.58088750898878 37.0 37.0 37.0 18.5 37.0 12-13 33.566050764775625 37.0 37.0 37.0 18.5 37.0 14-15 35.12265397547276 40.0 37.0 40.0 15.0 40.0 16-17 35.073277554272835 40.0 37.0 40.0 18.5 40.0 18-19 35.161135793831264 40.0 37.0 40.0 18.5 40.0 20-21 35.18885652925971 40.0 37.0 40.0 18.5 40.0 22-23 36.00593940100674 40.0 37.0 40.0 27.0 40.0 24-25 35.23172711017822 40.0 37.0 40.0 22.0 40.0 26-27 35.14979369521699 40.0 37.0 40.0 21.0 40.0 28-29 34.80354556585621 40.0 37.0 40.0 15.0 40.0 30-31 34.811470176666084 40.0 37.0 40.0 15.0 40.0 32-33 34.6177958525256 40.0 37.0 40.0 15.0 40.0 34-35 34.960034108798325 40.0 37.0 40.0 18.5 40.0 36-37 34.522409577672825 40.0 37.0 40.0 15.0 40.0 38-39 34.50055565273162 40.0 37.0 40.0 15.0 40.0 40-41 35.02948797932093 38.5 37.0 40.0 22.0 40.0 42-43 34.63816172040503 37.0 37.0 40.0 15.0 40.0 44-45 34.17105436028997 37.0 37.0 40.0 15.0 40.0 46-47 34.08273161914757 37.0 37.0 40.0 15.0 40.0 48-49 34.022517734631606 37.0 37.0 40.0 15.0 40.0 50-51 33.0690845043049 37.0 33.0 38.5 15.0 38.5 52-53 33.519543952733564 37.0 33.0 38.5 15.0 40.0 54-55 34.18731074961616 37.0 37.0 40.0 15.0 40.0 56-57 34.50735914329582 37.0 37.0 40.0 18.5 40.0 58-59 33.96202213670729 37.0 33.0 40.0 15.0 40.0 60-61 33.803020912288886 37.0 33.0 40.0 15.0 40.0 62-63 33.45092404718869 37.0 33.0 40.0 10.5 40.0 64-65 33.29792849785241 37.0 33.0 40.0 10.5 40.0 66-67 33.205386955085224 37.0 33.0 40.0 15.0 40.0 68-69 33.331276796299534 37.0 33.0 38.5 15.0 40.0 70-71 32.91918274930519 37.0 33.0 37.0 15.0 40.0 72-73 33.19941111305464 37.0 33.0 37.0 18.5 40.0 74-75 33.29637562435621 37.0 33.0 37.0 22.0 40.0 76-77 33.001306920879244 37.0 33.0 37.0 22.0 37.0 78-79 32.55018065030222 37.0 33.0 37.0 22.0 37.0 80-81 32.03930888383574 37.0 33.0 37.0 15.0 37.0 82-83 31.63701465415039 37.0 33.0 37.0 15.0 37.0 84-85 31.39924406740132 37.0 33.0 37.0 15.0 37.0 86-87 31.214331331506422 37.0 33.0 37.0 15.0 37.0 88-89 31.063387751928946 37.0 33.0 37.0 15.0 37.0 90-91 30.91868987231065 33.0 33.0 37.0 15.0 37.0 92-93 30.78934911472606 33.0 33.0 37.0 15.0 37.0 94-95 30.665371309738987 33.0 33.0 37.0 15.0 37.0 96-97 30.52653042582551 33.0 33.0 37.0 15.0 37.0 98-99 30.403035585874488 33.0 30.0 37.0 15.0 37.0 100-101 29.31834110741842 33.0 27.0 37.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.041482165550942085 2306 2 -0.2546562488133155 2306 3 -0.2092498123242521 2306 4 -0.1307880870411111 2306 5 -0.1287858432239588 2306 6 -0.16705160217959758 2306 7 0.3759150959366764 2306 8 -0.30401626445501506 2306 9 -0.1211702066923479 2306 10-11 -0.12280700678292078 2306 12-13 -0.04449426615736485 2306 14-15 0.2685756735749081 2306 16-17 0.1499608357326636 2306 18-19 0.17868857941942196 2306 20-21 -0.006965147937584959 2306 22-23 0.17675241629957839 2306 24-25 0.3107895337571094 2306 26-27 0.05293929839477585 2306 28-29 0.11449267470430158 2306 30-31 0.09765382522623867 2306 32-33 0.17639565341044516 2306 34-35 -0.0446164055807472 2306 36-37 0.16512326913004927 2306 38-39 -0.010501380893828127 2306 40-41 -0.05962604517341674 2306 42-43 0.3247808035896682 2306 44-45 0.2884671548426212 2306 46-47 0.11329975600558839 2306 48-49 0.12340832824502712 2306 50-51 0.13581842917854914 2306 52-53 0.05135879207595195 2306 54-55 0.22335476158934142 2306 56-57 0.2114510067481845 2306 58-59 0.4752428490643297 2306 60-61 0.3171803941159439 2306 62-63 0.3586760374254254 2306 64-65 0.27696719327792607 2306 66-67 0.22728801590975678 2306 68-69 0.17122879498197108 2306 70-71 0.2524472915916718 2306 72-73 -0.05058104367074634 2306 74-75 0.05900882080069891 2306 76-77 0.3536421047396274 2306 78-79 0.20578845019627323 2306 80-81 0.31203816371100856 2306 82-83 0.35245348383669395 2306 84-85 0.24229706602863743 2306 86-87 0.33130764300833704 2306 88-89 0.28445014037276195 2306 90-91 0.34520327697346787 2306 92-93 0.268518775138606 2306 94-95 0.34129706749484257 2306 96-97 0.2194792479457277 2306 98-99 0.14608807471452678 2306 100-101 0.2034723232676825 2307 1 -0.2536678629159219 2307 2 -0.4293625202526563 2307 3 -0.45628776091598766 2307 4 -0.29058180791052735 2307 5 -0.459135025644855 2307 6 -0.5073604824884796 2307 7 0.09928432173617807 2307 8 -0.5256361940032335 2307 9 -0.4433185246175597 2307 10-11 -0.4012619899391012 2307 12-13 -0.4001648656128296 2307 14-15 -0.07929915914056096 2307 16-17 -0.1918917539087701 2307 18-19 -0.2622639040828645 2307 20-21 -0.2616378855585353 2307 22-23 -0.2666588805977739 2307 24-25 -0.17451303973270882 2307 26-27 -0.5221467666116055 2307 28-29 -0.30171123552351276 2307 30-31 -0.1836525323550191 2307 32-33 -0.26686174171945964 2307 34-35 -0.5542203834915043 2307 36-37 -0.2471477241170419 2307 38-39 -0.41722368088306183 2307 40-41 -0.5115317043898315 2307 42-43 -0.16306780198802784 2307 44-45 -0.2350944289380834 2307 46-47 -0.27251566955087014 2307 48-49 -0.3613806240538864 2307 50-51 -0.4892124896173087 2307 52-53 -0.45069899992207496 2307 54-55 -0.38113915463764414 2307 56-57 -0.26789277642030385 2307 58-59 -0.16448895730094648 2307 60-61 -0.2491103502425389 2307 62-63 -0.19291558834946443 2307 64-65 -0.22962597074551638 2307 66-67 -0.31758015618948576 2307 68-69 -0.377478384024009 2307 70-71 -0.31983669985566365 2307 72-73 -0.5625262358748984 2307 74-75 -0.5791806606557159 2307 76-77 -0.2555773879460048 2307 78-79 -0.24710361090296118 2307 80-81 -0.15532958536988772 2307 82-83 -0.16509908580750832 2307 84-85 -0.11127236281967967 2307 86-87 -0.1847666697791226 2307 88-89 -0.018317630243615213 2307 90-91 -0.05956052496165043 2307 92-93 -0.11415239346881734 2307 94-95 -0.18409080187709037 2307 96-97 -0.1684731124966703 2307 98-99 -0.2352238205615116 2307 100-101 -0.2773122604053455 2201 1 0.0210296917816315 2201 2 -0.05589272392571587 2201 3 -0.016182826049146115 2201 4 0.04020142430127294 2201 5 -0.08795847494450015 2201 6 0.017881630372684754 2201 7 0.36551878749445166 2201 8 -0.10145738934824067 2201 9 0.09063161599924285 2201 10-11 0.030502155158380617 2201 12-13 0.005737158860632974 2201 14-15 0.2302683335221687 2201 16-17 0.1164466184772266 2201 18-19 0.20272972900600905 2201 20-21 0.09766420564832146 2201 22-23 0.08991759383583542 2201 24-25 0.09268337436846252 2201 26-27 -0.0029994560357238242 2201 28-29 0.04059024963511604 2201 30-31 0.06580314781493968 2201 32-33 0.11211410487543816 2201 34-35 -0.09803212039847864 2201 36-37 0.07815393808938609 2201 38-39 -0.029307737729027394 2201 40-41 0.005278609325245043 2201 42-43 0.20051244492555753 2201 44-45 0.16601692050659977 2201 46-47 0.026343223355638656 2201 48-49 0.02962749290661293 2201 50-51 -0.005577187648668769 2201 52-53 -0.13589320773533586 2201 54-55 0.10635864928872962 2201 56-57 0.27330638192170653 2201 58-59 0.29126799012266247 2201 60-61 0.1953437296877354 2201 62-63 0.3123099773522924 2201 64-65 0.20220351179825258 2201 66-67 0.09717606261434497 2201 68-69 0.11214991774714633 2201 70-71 0.20637910778871316 2201 72-73 0.03889501530825612 2201 74-75 0.06753932463569612 2201 76-77 0.2417839189391131 2201 78-79 0.26837458838475925 2201 80-81 0.2618554227675034 2201 82-83 0.23595349738793203 2201 84-85 0.22722984687300496 2201 86-87 0.17385606860552016 2201 88-89 0.1977621229790003 2201 90-91 0.22555915253898107 2201 92-93 0.2540776733326773 2201 94-95 0.3260024886923887 2201 96-97 0.18659089709114696 2201 98-99 0.05575753494365543 2201 100-101 0.08589124922673363 1101 1 0.16758902878319404 1101 2 -0.022473777015836305 1101 3 0.04030667945876587 1101 4 0.1686767802068374 1101 5 0.07750413848361148 1101 6 0.10120463041329941 1101 7 0.3934228111528384 1101 8 0.14294421236604649 1101 9 0.23514801610290448 1101 10-11 0.20238749406701828 1101 12-13 0.18963490562774865 1101 14-15 0.41173173623412396 1101 16-17 0.3169836621635085 1101 18-19 0.24266817492910064 1101 20-21 0.3489612585213493 1101 22-23 0.2910485772166709 1101 24-25 0.18238399282052598 1101 26-27 0.17278171393741815 1101 28-29 0.22705397762116064 1101 30-31 0.36487730275826635 1101 32-33 0.2955057898634763 1101 34-35 0.11334275025566143 1101 36-37 0.14200901464739957 1101 38-39 0.22999123731616322 1101 40-41 0.06874120046937549 1101 42-43 0.15307580238667384 1101 44-45 0.22155675148628262 1101 46-47 0.20264240578551096 1101 48-49 0.05337699376364924 1101 50-51 -0.011591523430006134 1101 52-53 0.052825244721994125 1101 54-55 0.37801192939932093 1101 56-57 0.26981256087133687 1101 58-59 0.20602003243058675 1101 60-61 0.2639514919134598 1101 62-63 0.3893373064854586 1101 64-65 0.3394302680896075 1101 66-67 0.2792983699829037 1101 68-69 0.27684741824045034 1101 70-71 0.4714356138274951 1101 72-73 0.1533308408880245 1101 74-75 0.21898720764054502 1101 76-77 0.22957895241717807 1101 78-79 0.16872435686509135 1101 80-81 0.3054367587202833 1101 82-83 0.30763195479086747 1101 84-85 0.32329864899098837 1101 86-87 0.31018616465302884 1101 88-89 0.3524078455888535 1101 90-91 0.1247856471733968 1101 92-93 0.3432222707817374 1101 94-95 0.3222419788351516 1101 96-97 0.328332673317675 1101 98-99 0.31945283183981843 1101 100-101 0.06324273824522919 2304 1 -0.06692574528623041 2304 2 -0.20241201034356493 2304 3 -0.09973287649128082 2304 4 -0.062271335398868644 2304 5 -0.10035868444248308 2304 6 -0.05404035307367394 2304 7 0.2779836537491178 2304 8 -0.16604768879342657 2304 9 -1.36298240072108E-4 2304 10-11 0.01693948873575124 2304 12-13 0.011764669748664858 2304 14-15 0.13113084983974233 2304 16-17 0.13677688514448505 2304 18-19 0.07247056183503986 2304 20-21 0.1446707170814321 2304 22-23 0.10555907017047161 2304 24-25 0.06843170063806525 2304 26-27 -0.017091716112766164 2304 28-29 -0.06721763860388563 2304 30-31 0.06344233902979113 2304 32-33 0.014749177347077591 2304 34-35 -0.1852683112554061 2304 36-37 -0.02038795342235744 2304 38-39 -0.15647733143848797 2304 40-41 -0.08183475892366232 2304 42-43 0.18816114657820293 2304 44-45 0.01612055709939142 2304 46-47 -0.06097751555709863 2304 48-49 -0.020921951908825065 2304 50-51 -0.0626654168276275 2304 52-53 -0.15520975783520186 2304 54-55 0.12388237349039599 2304 56-57 0.1237882628347009 2304 58-59 0.1712092921546997 2304 60-61 0.19621306374951786 2304 62-63 0.21604589475403202 2304 64-65 0.22758736685243264 2304 66-67 0.11754064736326342 2304 68-69 0.032506423268493734 2304 70-71 0.05368936148105519 2304 72-73 -0.004579342691322097 2304 74-75 -0.043222412922318654 2304 76-77 0.10301971621323247 2304 78-79 0.027881442425290004 2304 80-81 0.10984848300344652 2304 82-83 0.17684847167259576 2304 84-85 0.12271187859059651 2304 86-87 0.19155700255046781 2304 88-89 0.16877693359651147 2304 90-91 0.02820460931236468 2304 92-93 0.10876485240700973 2304 94-95 0.1421032711217194 2304 96-97 0.22568286186640307 2304 98-99 0.0723615230889223 2304 100-101 0.053978873696436835 2202 1 0.05496558599872259 2202 2 -0.17066257250174033 2202 3 -0.07294666938508954 2202 4 -0.07272045798415405 2202 5 -0.19271741295307976 2202 6 -0.019769402832373828 2202 7 0.051652392794935054 2202 8 0.037124695181205425 2202 9 -0.06943214262924613 2202 10-11 0.03962755074393698 2202 12-13 -5.309654560718968E-4 2202 14-15 0.07660985620167082 2202 16-17 0.08839383484342989 2202 18-19 0.07706232020821346 2202 20-21 0.10228127085623129 2202 22-23 0.09757721402056774 2202 24-25 0.13814881368485743 2202 26-27 0.1411062139612298 2202 28-29 0.0898659743753143 2202 30-31 0.1606839042573398 2202 32-33 0.047324003779998236 2202 34-35 -0.10084129262558861 2202 36-37 -0.0372773968477631 2202 38-39 -0.07519427889759811 2202 40-41 0.03563295147868928 2202 42-43 0.045719544233413956 2202 44-45 0.08566888270396333 2202 46-47 0.10806310782052009 2202 48-49 0.07967421373645323 2202 50-51 0.0402539759695415 2202 52-53 -0.020861803323022343 2202 54-55 0.1677708581871329 2202 56-57 0.18068497971896846 2202 58-59 0.12022818514905964 2202 60-61 0.10143541520657351 2202 62-63 0.26090405730673893 2202 64-65 0.22596313769508214 2202 66-67 0.1826666019922456 2202 68-69 0.10150074009131771 2202 70-71 0.12483246843389395 2202 72-73 0.14438382444904363 2202 74-75 0.25239714298272276 2202 76-77 0.21755589472154924 2202 78-79 0.20829561961050302 2202 80-81 0.26740981044068945 2202 82-83 0.23810176442761488 2202 84-85 0.20144447088573258 2202 86-87 0.22981260633354594 2202 88-89 0.27884714437283264 2202 90-91 0.28317233971322153 2202 92-93 0.2011976754049769 2202 94-95 0.3193905309495868 2202 96-97 0.2345289537171702 2202 98-99 0.18156879806766568 2202 100-101 0.29300254033564244 1102 1 0.1357492236621809 1102 2 8.928622484916104E-4 1102 3 0.09579309112249135 1102 4 0.12076610780179209 1102 5 0.13642269309082877 1102 6 0.23176801041120143 1102 7 0.26323530661936445 1102 8 0.26019408542874345 1102 9 0.09211093026104322 1102 10-11 0.18239753823313976 1102 12-13 0.2946410207820449 1102 14-15 0.31205562784982277 1102 16-17 0.2874726528708891 1102 18-19 0.21132347566977217 1102 20-21 0.23870109749231716 1102 22-23 0.16125568651170852 1102 24-25 0.06927761532912768 1102 26-27 0.2416284280745984 1102 28-29 0.22141347817323975 1102 30-31 0.209196757612915 1102 32-33 0.13290177179405305 1102 34-35 0.11805801779113523 1102 36-37 0.15154387001947356 1102 38-39 0.10023280102257104 1102 40-41 0.1873207921745319 1102 42-43 0.173266943774685 1102 44-45 0.19651287752091662 1102 46-47 0.08807468775557936 1102 48-49 0.183461336550792 1102 50-51 0.09207530241201312 1102 52-53 0.1820970376409079 1102 54-55 0.3384680373000606 1102 56-57 0.39962096613580655 1102 58-59 0.3440027971653876 1102 60-61 0.3367817706919638 1102 62-63 0.38031906579620767 1102 64-65 0.3639659898591532 1102 66-67 0.40358681586731393 1102 68-69 0.3449437414913348 1102 70-71 0.34889955758821856 1102 72-73 0.2907759858383372 1102 74-75 0.21026963709966395 1102 76-77 0.22710412831602156 1102 78-79 0.24485470334348491 1102 80-81 0.2642942121564502 1102 82-83 0.3216966081915764 1102 84-85 0.25115720658022767 1102 86-87 0.35732277135693025 1102 88-89 0.39207116957315336 1102 90-91 0.43793947503835184 1102 92-93 0.4344458658074082 1102 94-95 0.36427097091264926 1102 96-97 0.2325043268070388 1102 98-99 0.27577194215035306 1102 100-101 0.2849615600252555 2305 1 0.1583023042734979 2305 2 -0.016504865534916036 2305 3 0.06636251938675031 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18953.0 17 17103.0 18 16424.0 19 16553.0 20 17407.0 21 17810.0 22 18883.0 23 19718.0 24 21595.0 25 24069.0 26 28064.0 27 33605.0 28 41006.0 29 49273.0 30 59673.0 31 77121.0 32 104637.0 33 150447.0 34 230429.0 35 398811.0 36 835809.0 37 1701634.0 38 736270.0 39 977.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.766257600023366 23.736918710782906 25.00820242713957 12.48862126205416 2 29.126973401601656 26.963957392401248 33.49280740706411 10.416261798932979 3 36.40753934194766 19.697867235149594 29.864178080871774 14.030415342030977 4 25.28886849594489 18.04018919754955 38.68419049562057 17.98675181088499 5 33.97062098847922 17.27732117241927 33.83899326695293 14.91306457214858 6 28.266936055987706 17.5494436349959 40.777945378187255 13.40567493082914 7 35.38389410698209 19.23691760611157 30.606688803612386 14.772499483293949 8 25.606174141278835 32.70996631603772 27.922002490250993 13.76185705243245 9 34.61039957954766 26.247449275931967 24.10601827862439 15.03613286589598 10-11 26.754707041239172 30.411319815786182 28.13023130222548 14.703741840749165 12-13 29.41444140803738 24.260789717122382 27.497047985633365 18.827720889206866 14-15 25.25182660821157 25.180841436971036 29.795607267145822 19.771724687671572 16-17 26.64955080594204 26.346096129328195 30.833979880270046 16.17037318445972 18-19 25.651357449511064 27.08094651768786 31.068507965218906 16.199188067582167 20-21 24.345822608522408 29.43476991565651 29.822356370384473 16.39705110543661 22-23 27.6646030285284 27.026833781146703 28.945851873325562 16.36271131699933 24-25 23.428543757244807 31.194986937502005 28.85768872427053 16.518780580982657 26-27 21.970163553000752 32.87311204576653 28.462860314636128 16.693864086596584 28-29 23.16952095357366 31.138934820166853 28.466677567892322 17.224866658367166 30-31 25.523818673178788 28.034525766639778 28.993981891013448 17.447673669167983 32-33 21.389025792592854 30.55215069519414 30.923198987511423 17.13562452470158 34-35 26.374113548631357 28.184618365937887 28.887258192793837 16.554009892636923 36-37 22.535315040972296 29.91043604864496 31.101220743626683 16.453028166756052 38-39 22.09917196298917 32.29866978376222 28.757156629273805 16.845001623974802 40-41 26.88228321578331 27.751679203634954 28.026547061626424 17.339490518955316 42-43 26.4547622598693 27.89163734893937 28.840440193640877 16.81316019755045 44-45 22.946483519140635 30.1268931401967 30.046563624408474 16.88005971625419 46-47 23.704087437240524 30.552107859261955 28.725772084319246 17.018032619178275 48-49 20.26781597627735 30.39188438563191 30.62150056209447 18.718799075996266 50-51 19.066140952944227 28.371316754481768 32.3241765176219 20.238365774952108 52-53 24.732902476818683 27.886375044507723 28.521590791507457 18.859131687166144 54-55 25.894882805693996 27.788335472009855 28.49518585718126 17.821595865114887 56-57 26.37596557918759 27.602941317008373 28.111263041060287 17.909830062743755 58-59 24.766777751114738 27.65705357317849 28.49800220582765 19.078166469879122 60-61 20.97303992264841 28.109998885814292 27.996172582004995 22.920788609532302 62-63 23.904688528652702 28.209425684413386 28.39808482505696 19.487800961876953 64-65 20.84041147165841 29.670437980007453 30.86198295631411 18.627167592020022 66-67 19.37700443541283 29.48233756815434 29.511578084023725 21.629079912409104 68-69 18.691033010887683 28.317003725806096 34.62199806856271 18.36996519474351 70-71 18.13694249821081 32.70416575119658 31.367943333977333 17.79094841661528 72-73 17.96835329907998 36.26103099623933 28.02845228541639 17.742163419264305 74-75 18.365736279635826 36.10449012023832 27.69748655338323 17.832287046742625 76-77 18.839387725844002 35.581737344534666 27.891840675081603 17.687034254539736 78-79 18.82903682690554 35.30486804618961 28.272260239424057 17.59383488748079 80-81 18.278761553653204 35.4973651529537 28.063957670884893 18.1599156225082 82-83 17.930284700787986 34.85979472213677 28.26350411545405 18.94641646162119 84-85 18.165857830712795 33.93845631530201 28.91501513684982 18.98067071713538 86-87 18.220345303806802 33.49192459805796 29.59606659604661 18.69166350208862 88-89 18.6159731528965 33.757399023032534 28.99036293826633 18.63626488580464 90-91 19.07863665524631 33.054724107740036 28.790791046493265 19.07584819052039 92-93 18.72063507444143 33.62279285086983 28.69013361897152 18.966438455717228 94-95 18.530959989371283 33.72857332377165 28.623991482989712 19.116475203867356 96-97 19.137939318617267 33.69043475820122 28.2990030677612 18.872622855420314 98-99 19.510587097588665 32.8681161687872 28.03133747741398 19.58995925621015 100-101 19.45016969734074 32.85179466612021 27.75215901772663 19.945876618812413 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1290.0 1 1131.5 2 823.0 3 788.0 4 914.0 5 951.5 6 1100.0 7 1405.0 8 1773.0 9 2123.5 10 2441.0 11 2569.5 12 2624.0 13 2596.5 14 2372.0 15 2314.0 16 2386.5 17 2480.5 18 2765.5 19 3361.5 20 4250.5 21 5630.5 22 7877.0 23 11014.0 24 15275.5 25 20820.0 26 28544.5 27 38323.5 28 49341.0 29 61313.5 30 73805.5 31 86809.5 32 102687.0 33 122705.5 34 147308.0 35 181727.0 36 229750.5 37 287756.0 38 340407.5 39 354441.5 40 344303.5 41 312251.0 42 266192.5 43 251422.5 44 236615.5 45 220020.0 46 225444.5 47 207479.5 48 155519.0 49 114353.0 50 92176.5 51 78863.5 52 69134.5 53 58595.5 54 50069.0 55 43447.5 56 37198.0 57 31496.5 58 26734.0 59 22509.0 60 18735.5 61 15412.5 62 12712.0 63 10486.5 64 8323.0 65 6539.5 66 5201.5 67 4030.0 68 3043.0 69 2366.5 70 1819.0 71 1379.0 72 1031.5 73 741.5 74 544.0 75 416.0 76 321.0 77 220.0 78 150.5 79 129.0 80 105.5 81 74.5 82 65.5 83 50.5 84 35.0 85 31.5 86 28.5 87 28.5 88 22.5 89 16.0 90 13.5 91 11.5 92 9.5 93 8.5 94 6.0 95 6.5 96 5.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18706392241463082 2 0.19198103123238686 3 0.16234233183682195 4 0.16416924183235185 5 0.17188502128155791 6 0.1699415000097176 7 0.13569665519989116 8 0.11775795386080501 9 0.11803004683886265 10-11 0.09657357199774552 12-13 0.14154665422813054 14-15 0.12046916603502225 16-17 0.11306434998931063 18-19 0.11575612695080947 20-21 0.10773910170446814 22-23 0.10708802207840165 24-25 0.4656579791265815 26-27 0.3136260276368725 28-29 0.39830524945095525 30-31 0.26312362738810174 32-33 0.29095485200085514 34-35 0.3095932209978038 36-37 0.2904301012574583 38-39 0.3391347443297767 40-41 0.3342759411501759 42-43 0.377422113385031 44-45 0.3313315064233378 46-47 0.3553825821623618 48-49 0.350766719141741 50-51 0.3655569160204459 52-53 0.35722892737061007 54-55 0.3328960410471693 56-57 0.3486094105299983 58-59 0.34049520922006493 60-61 0.31083707461178167 62-63 0.3201659767166152 64-65 0.32142926554331136 66-67 0.3331486988125085 68-69 0.3202339999611296 70-71 0.3085728723300876 72-73 0.2934134064097332 74-75 0.3026256972382563 76-77 0.2918488717859017 78-79 0.30185800633587934 80-81 0.31038034711289914 82-83 0.33763823295045964 84-85 0.33528657221153285 86-87 0.3287952111635862 88-89 0.3419820029930228 90-91 0.33098167259440653 92-93 0.3148601636444911 94-95 0.3070860785571298 96-97 0.2917516957223097 98-99 0.3167453792781762 100-101 0.3199619069830719 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5145300.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.990936272131904 #Duplication Level Percentage of deduplicated Percentage of total 1 93.80592786745629 56.27505440644771 2 3.278213644479656 3.9332621166482458 3 0.8762089778505937 1.576937908539144 4 0.482648103015414 1.158180463594522 5 0.24626910755677778 0.7386957168616725 6 0.13272131516060184 0.47772455758519194 7 0.13825814193004507 0.580596476714009 8 0.10920996281772825 0.5241286335744184 9 0.08026223101914065 0.43335057475155553 >10 0.6554193325599278 7.744480252081415 >50 0.09046295987530757 3.844351019030733 >100 0.08418091088143978 10.342443539444453 >500 0.014129862220220942 5.93045294721062 >1k 0.005858536828331254 5.340697977994775 >5k 1.9632599033068995E-4 0.8595188013772731 >10k+ 3.272035850565363E-5 0.24012460814433664 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 12233 0.2377509571842264 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9047 0.17583036946339378 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8128 0.15796940897518122 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7868 0.1529162536683964 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6909 0.13427788467144774 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6509 0.12650379958408645 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5326 0.10351194293821546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018852156336851107 0.0 0.0 2 0.0021184381863059492 0.0 0.0 3 0.002235049462616368 0.0 0.0 4 0.002273919888053175 0.0 0.0 5 0.002565448078829223 0.0 0.0 6 0.0027403649932948517 0.0 0.0 7 0.002993022758634093 0.0 0.0 8 0.00351777350203098 0.0 0.0 9 0.0038481721182438344 0.0 0.0 10-11 0.006530231473383476 0.0 9.717606359201602E-6 12-13 0.008561211202456611 0.0 4.858803179600801E-5 14-15 0.010660214176044156 0.0 5.830563815520961E-5 16-17 0.011758303694633938 0.0 5.830563815520961E-5 18-19 0.012934134064097332 0.0 7.774085087361282E-5 20-21 0.014177987678075137 0.0 7.774085087361282E-5 22-23 0.015616193419236974 0.0 9.717606359201602E-5 24-25 0.017326492138456454 0.0 1.1661127631041922E-4 26-27 0.019065943676753544 0.0 1.3604648902882242E-4 28-29 0.02135929877752512 0.0 1.3604648902882242E-4 30-31 0.03888986064952481 0.0 1.5548170174722563E-4 32-33 0.04224243484344936 0.0 1.5548170174722563E-4 34-35 0.04442889627426972 0.0 1.7491691446562882E-4 36-37 0.04654733446057567 0.0 1.9435212718403204E-4 38-39 0.04901560647581288 0.0 2.0406973354323362E-4 40-41 0.05175597146910773 0.0 2.2350494626163683E-4 42-43 0.055536120342837156 0.0 2.526577653392416E-4 44-45 0.059500903737391406 0.0 2.623753716984432E-4 46-47 0.06338794628107204 0.0 3.0124579713524963E-4 48-49 0.06807183254620722 0.0 3.1096340349445127E-4 50-51 0.07312498785299205 0.0 3.2068100985365285E-4 52-53 0.07896526927487221 0.0 3.3039861621285443E-4 54-55 0.08515538452568364 0.0 3.3039861621285443E-4 56-57 0.09209375546615359 0.0 3.3039861621285443E-4 58-59 0.10045089693506695 0.0 3.3039861621285443E-4 60-61 0.10994499834800692 0.0 3.3039861621285443E-4 62-63 0.11987639204711095 0.0 3.4011622257205606E-4 64-65 0.13144034361456086 0.0 3.4983382893125764E-4 66-67 0.1444910889549686 0.0 3.595514352904592E-4 68-69 0.15929100344003266 0.0 3.8870425436806407E-4 70-71 0.17511126659281284 0.0 3.9842186072726565E-4 72-73 0.19313742638913184 0.0 4.1785707344566887E-4 74-75 0.2141954793695217 0.0 4.372922861640721E-4 76-77 0.23763434590791596 0.0 4.5672749888247524E-4 78-79 0.2634540260043146 0.0 5.053155306784832E-4 80-81 0.29334538316521874 0.0 5.441859561152897E-4 82-83 0.32669620818999867 0.0 5.441859561152897E-4 84-85 0.3638854877266632 0.0 5.733387751928945E-4 86-87 0.4046411287971547 0.0 5.927739879112977E-4 88-89 0.4519464365537481 0.0 6.024915942704993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5525 0.0 87.85401 9 CGGGAGA 8090 0.0 87.38676 4 TCGGGAG 8595 0.0 85.893295 3 AGAGCGG 5925 0.0 85.283066 8 GAGAGCG 7450 0.0 82.52511 7 GTTCGGA 915 0.0 80.3308 2 AAGCGGC 1470 0.0 79.00284 9 GATCGGG 10480 0.0 78.78716 1 ATCCGGA 1250 0.0 78.14994 2 ATCGGGA 10460 0.0 77.342606 2 AGAGCGT 13295 0.0 76.40605 8 TCGGAGA 1560 0.0 76.28966 4 ATTCGGA 1360 0.0 76.013214 2 GAGCGTC 13230 0.0 75.81406 9 CGGAGAG 2505 0.0 74.38773 5 GATCCGG 1365 0.0 74.00566 1 CGGAAAG 2465 0.0 73.2866 5 TTCGGAG 1265 0.0 73.09045 3 AAGAGCG 12860 0.0 72.28348 7 TCGGGAA 2010 0.0 71.00468 3 >>END_MODULE