FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145300
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT122330.2377509571842264Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC90470.17583036946339378No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC81280.15796940897518122No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG78680.1529162536683964No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69090.13427788467144774No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA65090.12650379958408645No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA53260.10351194293821546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC55250.087.854019
CGGGAGA80900.087.386764
TCGGGAG85950.085.8932953
AGAGCGG59250.085.2830668
GAGAGCG74500.082.525117
GTTCGGA9150.080.33082
AAGCGGC14700.079.002849
GATCGGG104800.078.787161
ATCCGGA12500.078.149942
ATCGGGA104600.077.3426062
AGAGCGT132950.076.406058
TCGGAGA15600.076.289664
ATTCGGA13600.076.0132142
GAGCGTC132300.075.814069
CGGAGAG25050.074.387735
GATCCGG13650.074.005661
CGGAAAG24650.073.28665
TTCGGAG12650.073.090453
AAGAGCG128600.072.283487
TCGGGAA20100.071.004683
Produced by FastQC (version 0.11.2)