##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_52260024-1.3420000003582d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6454864 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67366221813504 33.0 33.0 33.0 27.0 33.0 2 31.528037306440538 33.0 33.0 33.0 27.0 33.0 3 31.441838898542247 33.0 33.0 33.0 27.0 33.0 4 35.07847369053786 37.0 37.0 37.0 33.0 37.0 5 35.12581612873641 37.0 37.0 37.0 33.0 37.0 6 35.2482628603794 37.0 37.0 37.0 33.0 37.0 7 34.98931611882141 37.0 37.0 37.0 33.0 37.0 8 35.0806449214112 37.0 37.0 37.0 33.0 37.0 9 34.85590199886473 37.0 37.0 37.0 33.0 37.0 10-11 34.799304524464034 37.0 37.0 37.0 33.0 37.0 12-13 34.77604841868086 37.0 37.0 37.0 33.0 37.0 14-15 36.37598483872007 40.0 37.0 40.0 33.0 40.0 16-17 36.33141418626326 40.0 37.0 40.0 33.0 40.0 18-19 36.345394961071214 40.0 37.0 40.0 33.0 40.0 20-21 36.369465801293416 40.0 37.0 40.0 33.0 40.0 22-23 36.539742897758956 40.0 37.0 40.0 33.0 40.0 24-25 36.18370766603293 40.0 37.0 40.0 27.0 40.0 26-27 36.097024894715055 40.0 37.0 40.0 27.0 40.0 28-29 35.95099060181593 40.0 37.0 40.0 27.0 40.0 30-31 35.98051360958186 40.0 37.0 40.0 27.0 40.0 32-33 35.85469964975249 40.0 37.0 40.0 27.0 40.0 34-35 35.8704360773519 38.5 37.0 40.0 27.0 40.0 36-37 35.75640044468791 37.0 37.0 40.0 27.0 40.0 38-39 35.75008017209967 37.0 37.0 40.0 27.0 40.0 40-41 35.47838227110594 37.0 37.0 40.0 27.0 40.0 42-43 35.39578587558158 37.0 37.0 40.0 27.0 40.0 44-45 35.26964185457664 37.0 37.0 40.0 27.0 40.0 46-47 35.20117309985152 37.0 37.0 40.0 27.0 40.0 48-49 35.088233384932664 37.0 37.0 40.0 27.0 40.0 50-51 33.940719742507355 37.0 33.0 38.5 24.5 38.5 52-53 34.42113234298972 37.0 35.0 38.5 27.0 40.0 54-55 35.166892439561856 37.0 37.0 40.0 27.0 40.0 56-57 35.2327978560044 37.0 37.0 40.0 27.0 40.0 58-59 34.9250138345285 37.0 35.0 40.0 27.0 40.0 60-61 34.71687172649958 37.0 33.0 40.0 27.0 40.0 62-63 34.40132526417288 37.0 33.0 40.0 27.0 40.0 64-65 34.203428763177655 37.0 33.0 37.0 27.0 40.0 66-67 33.97986828227519 37.0 33.0 37.0 27.0 40.0 68-69 33.83611041224106 37.0 33.0 37.0 27.0 40.0 70-71 33.471711410186174 37.0 33.0 37.0 22.0 38.5 72-73 33.35369095305494 37.0 33.0 37.0 27.0 37.0 74-75 33.184982596070185 37.0 33.0 37.0 27.0 37.0 76-77 32.91596647117585 37.0 33.0 37.0 22.0 37.0 78-79 32.637837838256544 37.0 33.0 37.0 22.0 37.0 80-81 32.3456164994336 37.0 33.0 37.0 22.0 37.0 82-83 32.11111721331387 35.0 33.0 37.0 22.0 37.0 84-85 31.95099184119139 33.0 33.0 37.0 22.0 37.0 86-87 31.796776275999 33.0 33.0 37.0 22.0 37.0 88-89 31.65867553522429 33.0 33.0 37.0 18.5 37.0 90-91 31.52192509400663 33.0 33.0 37.0 15.0 37.0 92-93 31.384739941848505 33.0 33.0 37.0 15.0 37.0 94-95 31.24189363246073 33.0 33.0 37.0 15.0 37.0 96-97 31.0636504502651 33.0 33.0 37.0 15.0 37.0 98-99 30.913747446886564 33.0 33.0 37.0 15.0 37.0 100-101 29.647910320031528 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.21088793252075533 2306 2 0.19577142542432568 2306 3 0.18934093085121617 2306 4 0.29258049449049395 2306 5 0.26609369456929244 2306 6 0.2486410466290323 2306 7 0.43589815925921727 2306 8 0.21017687227443815 2306 9 0.2754187999801374 2306 10-11 0.2989700497203387 2306 12-13 0.32997190880061567 2306 14-15 0.6737777384973356 2306 16-17 0.5956986025331474 2306 18-19 0.6517083670806016 2306 20-21 0.6346215423886505 2306 22-23 0.486082868052101 2306 24-25 0.5160443703106239 2306 26-27 0.5331600596152057 2306 28-29 0.454054376307262 2306 30-31 0.3543242977154577 2306 32-33 0.3751007115265139 2306 34-35 0.33677670969030515 2306 36-37 0.3744755473418664 2306 38-39 0.26627052663845063 2306 40-41 0.34068079215877134 2306 42-43 0.35046439976703425 2306 44-45 0.46549727898793947 2306 46-47 0.3466454202664835 2306 48-49 0.32109611511840797 2306 50-51 0.2891637105753304 2306 52-53 0.3309723054887428 2306 54-55 0.403667189646427 2306 56-57 0.3724988625716179 2306 58-59 0.5392647481767057 2306 60-61 0.428089670727438 2306 62-63 0.5053666073383098 2306 64-65 0.43805277449453683 2306 66-67 0.4590914075748458 2306 68-69 0.30053117006738006 2306 70-71 0.40243058142357313 2306 72-73 0.3918571631614398 2306 74-75 0.43799280765316695 2306 76-77 0.5520765982388909 2306 78-79 0.4932839828051314 2306 80-81 0.5505207291769167 2306 82-83 0.4345351695385773 2306 84-85 0.4196477247563948 2306 86-87 0.4570481988670423 2306 88-89 0.44782437468702696 2306 90-91 0.367657656036549 2306 92-93 0.3265727620489578 2306 94-95 0.36560034550877774 2306 96-97 0.31754689198708164 2306 98-99 0.28948355883386867 2306 100-101 0.26941364128588674 2307 1 0.228229639962386 2307 2 0.18209594908751114 2307 3 0.1708852210126608 2307 4 0.2788758264315234 2307 5 0.15860639163344104 2307 6 0.21063146640421593 2307 7 0.3455996632202982 2307 8 0.14767592665844376 2307 9 0.2128012654738285 2307 10-11 0.3290510291595794 2307 12-13 0.31104416970483584 2307 14-15 0.5547350970451959 2307 16-17 0.522285826775942 2307 18-19 0.5556202981468417 2307 20-21 0.41892517563666587 2307 22-23 0.38778708613136104 2307 24-25 0.3271909713172789 2307 26-27 0.3445433538690139 2307 28-29 0.26225212388109753 2307 30-31 0.3412295853509306 2307 32-33 0.3234299170791317 2307 34-35 0.20711315407803 2307 36-37 0.111922394822777 2307 38-39 0.10563417557524701 2307 40-41 0.3363051933057264 2307 42-43 0.20384334605621746 2307 44-45 0.20940312874206057 2307 46-47 0.09774685153305995 2307 48-49 0.10279153919136519 2307 50-51 0.06009108263860696 2307 52-53 0.06816675137200434 2307 54-55 0.15865808414308447 2307 56-57 0.15463607843959437 2307 58-59 0.2879211947146416 2307 60-61 0.27613558752640444 2307 62-63 0.2658503515540289 2307 64-65 0.18298057927169253 2307 66-67 0.3190284566940136 2307 68-69 0.188565941502425 2307 70-71 0.14262623449134537 2307 72-73 0.06410122874242319 2307 74-75 0.027373121681335988 2307 76-77 0.2304444062840929 2307 78-79 0.2522090181066474 2307 80-81 0.2740608805217519 2307 82-83 0.04617466120389224 2307 84-85 0.07962187762240802 2307 86-87 0.08280135419850865 2307 88-89 0.26606207220061506 2307 90-91 0.29576887952392283 2307 92-93 0.13165151205941683 2307 94-95 0.17711709399236497 2307 96-97 0.18568341081369866 2307 98-99 0.16643766873464827 2307 100-101 -0.10897437846693947 2201 1 0.3568194762133068 2201 2 0.26425136369593005 2201 3 0.27858682947935165 2201 4 0.36058295611805846 2201 5 0.2536655590778736 2201 6 0.33257302740796035 2201 7 0.4459198057609157 2201 8 0.30034118859626346 2201 9 0.37419747204200604 2201 10-11 0.43960803829173756 2201 12-13 0.384610973998889 2201 14-15 0.5598195325357977 2201 16-17 0.5747840696792679 2201 18-19 0.5599327137303192 2201 20-21 0.4994332231868768 2201 22-23 0.4182088675855198 2201 24-25 0.4254022421558261 2201 26-27 0.4026264254162797 2201 28-29 0.4541659104268163 2201 30-31 0.4434221266296632 2201 32-33 0.46056467335251483 2201 34-35 0.3275700750945205 2201 36-37 0.3177464115125801 2201 38-39 0.3276341523369837 2201 40-41 0.3788009084559292 2201 42-43 0.31366325194602496 2201 44-45 0.283901715950968 2201 46-47 0.31678377285307135 2201 48-49 0.32709423738997145 2201 50-51 0.1838020115428236 2201 52-53 0.25467793988073595 2201 54-55 0.42497833481272806 2201 56-57 0.5232500042251473 2201 58-59 0.4521868516079621 2201 60-61 0.4423414016431195 2201 62-63 0.5752705120213051 2201 64-65 0.4642408349304432 2201 66-67 0.5708693770807045 2201 68-69 0.5053509691417233 2201 70-71 0.5053103739550266 2201 72-73 0.36462884385241523 2201 74-75 0.43337876430921085 2201 76-77 0.4321826989457378 2201 78-79 0.4377782866042921 2201 80-81 0.5308188261081952 2201 82-83 0.4414765972825663 2201 84-85 0.3171834827124371 2201 86-87 0.44327293902857434 2201 88-89 0.35615489243326337 2201 90-91 0.3976414798183434 2201 92-93 0.30576473548054395 2201 94-95 0.3917396359540142 2201 96-97 0.2860232940153793 2201 98-99 0.25493546726561434 2201 100-101 0.3041926780333206 1101 1 0.12168627657741737 1101 2 5.7139710040843283E-5 1101 3 0.1145690434521569 1101 4 0.15809831710116384 1101 5 0.18412994435409047 1101 6 0.1962450135261804 1101 7 0.30144424821412485 1101 8 0.2798635402489964 1101 9 0.2672867008637567 1101 10-11 0.2900030324602554 1101 12-13 0.19538597471004238 1101 14-15 0.41152303711564286 1101 16-17 0.3510200421148397 1101 18-19 0.29094321395788825 1101 20-21 0.32179200526004337 1101 22-23 0.3431759588126937 1101 24-25 0.19133065712993869 1101 26-27 0.2622229862628416 1101 28-29 0.23520937276395415 1101 30-31 0.2816796328878368 1101 32-33 0.28200921024732395 1101 34-35 0.23119192467066796 1101 36-37 0.09301286412111409 1101 38-39 0.15957388536878625 1101 40-41 0.12710289756100934 1101 42-43 0.01936443459949544 1101 44-45 -0.04118580965067764 1101 46-47 0.006598516552216438 1101 48-49 -0.13239780121161004 1101 50-51 -0.027285720294237592 1101 52-53 0.04946373950524219 1101 54-55 0.23846382010600564 1101 56-57 0.24478537359989616 1101 58-59 0.03577623688417475 1101 60-61 0.190144105460611 1101 62-63 0.35638011192458663 1101 64-65 0.17384637412939696 1101 66-67 0.08077874016882447 1101 68-69 0.204309391369641 1101 70-71 0.3135076452470571 1101 72-73 0.22462850605301554 1101 74-75 0.29249966804705707 1101 76-77 0.24162924130222763 1101 78-79 0.10563491387288337 1101 80-81 0.18896167613948478 1101 82-83 0.3248617739338755 1101 84-85 0.2084138547895833 1101 86-87 0.09450032424789612 1101 88-89 0.22788342972632236 1101 90-91 -0.03546531457205404 1101 92-93 0.17305590433757345 1101 94-95 0.153982037245477 1101 96-97 0.32009116324789844 1101 98-99 0.17954699795913243 1101 100-101 0.019095925174468675 2304 1 0.065277818167921 2304 2 0.028697403077956096 2304 3 0.014078285786684575 2304 4 0.1087207587251342 2304 5 0.11762384930479186 2304 6 0.17416124038816605 2304 7 0.17425128551813884 2304 8 0.20834885291794336 2304 9 0.1545368488955816 2304 10-11 0.12859574865107248 2304 12-13 0.10616019722117898 2304 14-15 0.1519648960091402 2304 16-17 0.21171215523689568 2304 18-19 0.22199292046872188 2304 20-21 0.2483431695624958 2304 22-23 0.21140635103265737 2304 24-25 0.04106754665958334 2304 26-27 0.08869744755573095 2304 28-29 0.059070235725108944 2304 30-31 0.08480633847185715 2304 32-33 0.029033835018836385 2304 34-35 -0.11785611809121832 2304 36-37 -0.05071596089241126 2304 38-39 -0.08780667547872412 2304 40-41 0.036702423215565716 2304 42-43 -0.014206881339021038 2304 44-45 -0.10296494295654668 2304 46-47 -0.0705478965724069 2304 48-49 -0.07608712425565045 2304 50-51 -0.04451815277885629 2304 52-53 -0.08626583210353544 2304 54-55 0.14784284270982084 2304 56-57 0.08846193694464688 2304 58-59 0.10350547703041002 2304 60-61 0.18196784139758648 2304 62-63 0.14113981766306694 2304 64-65 0.08320755246268874 2304 66-67 0.11381522679447187 2304 68-69 0.16336024995419507 2304 70-71 0.12328025549857813 2304 72-73 0.1605215562808624 2304 74-75 0.08054085307395553 2304 76-77 0.16235590054584748 2304 78-79 0.06860681357166953 2304 80-81 0.07388106315902832 2304 82-83 0.0817233384371292 2304 84-85 0.12225732401183365 2304 86-87 0.07671748692843039 2304 88-89 0.14008826714876577 2304 90-91 0.1345716129309693 2304 92-93 0.16983618006292645 2304 94-95 0.19258419851005826 2304 96-97 0.17343201616862913 2304 98-99 0.12443185494752029 2304 100-101 0.07270149760708478 2202 1 0.16012898536999387 2202 2 0.08692617534040536 2202 3 0.11886052777482803 2202 4 0.07476976377163425 2202 5 0.06737774810476793 2202 6 0.16486900667020166 2202 7 0.18887453982529223 2202 8 0.24739475336446048 2202 9 0.2286537819521115 2202 10-11 0.2229207993786062 2202 12-13 0.21018542338533308 2202 14-15 0.2873450817506722 2202 16-17 0.3024253013565641 2202 18-19 0.1578609777823914 2202 20-21 0.21301014823630027 2202 22-23 0.2804642734823588 2202 24-25 0.3003241492089046 2202 26-27 0.23494921270507518 2202 28-29 0.21370985927243424 2202 30-31 0.15103193463756526 2202 32-33 -0.04591878959180207 2202 34-35 -0.027364699811307958 2202 36-37 -0.12619654800030133 2202 38-39 -0.019502692148485323 2202 40-41 0.11851565939770836 2202 42-43 -0.0051410051685891744 2202 44-45 0.0178702246787239 2202 46-47 0.07354522337710989 2202 48-49 0.029481142484897305 2202 50-51 0.02917826802337231 2202 52-53 0.08999739831197928 2202 54-55 0.17423062398508193 2202 56-57 0.25940686381635913 2202 58-59 0.08693273482754194 2202 60-61 0.18478794876929783 2202 62-63 0.25621565813670344 2202 64-65 0.1687158521808314 2202 66-67 0.22110214068518985 2202 68-69 0.22293802529404871 2202 70-71 0.19835876127739027 2202 72-73 0.2008151922728345 2202 74-75 0.24090121311753876 2202 76-77 0.2537490569704275 2202 78-79 0.2362288409476605 2202 80-81 0.22208885053846217 2202 82-83 0.10045402259307679 2202 84-85 0.24623196094898248 2202 86-87 0.2664903218192194 2202 88-89 0.23959592409937258 2202 90-91 0.32128347843830696 2202 92-93 0.14923907903356337 2202 94-95 0.19650872560005794 2202 96-97 0.12383735587431843 2202 98-99 0.16249731221730812 2202 100-101 0.17042120830074836 1102 1 0.0349034020424952 1102 2 -0.08867193076241975 1102 3 -0.01796584975064519 1102 4 0.11680642983663603 1102 5 0.049817510082100114 1102 6 0.1473372080103701 1102 7 0.08658637115915724 1102 8 0.145390485158579 1102 9 0.04301099152786492 1102 10-11 0.10671501477932566 1102 12-13 0.173932879870641 1102 14-15 0.13785743413311735 1102 16-17 0.19388055506033908 1102 18-19 0.18053594479765422 1102 20-21 0.13672722885187483 1102 22-23 0.15191079153444775 1102 24-25 0.035968846549970124 1102 26-27 0.15735241119637777 1102 28-29 0.05811029667969336 1102 30-31 -0.01254018413441571 1102 32-33 0.026518569012786486 1102 34-35 -0.060334469590550555 1102 36-37 0.005670284107843315 1102 38-39 0.05622598843932991 1102 40-41 0.061562547523173805 1102 42-43 0.02776161154869783 1102 44-45 -0.0027105038226764577 1102 46-47 -0.09134044874038239 1102 48-49 -0.012034162659602998 1102 50-51 -0.03545404718077094 1102 52-53 0.08596172121349355 1102 54-55 0.18049122915499538 1102 56-57 0.28592319968227997 1102 58-59 0.27451310068596513 1102 60-61 0.2990165400936817 1102 62-63 0.2295445496689723 1102 64-65 0.25608897234716466 1102 66-67 0.174395747680812 1102 68-69 0.17647599710389983 1102 70-71 0.2011709704109279 1102 72-73 0.2671338959376399 1102 74-75 0.17317804877977494 1102 76-77 0.20836871090448028 1102 78-79 0.08343770653271321 1102 80-81 0.16078985880429286 1102 82-83 0.29275598024045735 1102 84-85 0.23890487967256746 1102 86-87 0.22788520187909 1102 88-89 0.353069228414693 1102 90-91 0.3959499570435234 1102 92-93 0.3283156644294074 1102 94-95 0.3459484537131736 1102 96-97 0.28404393085773805 1102 98-99 0.2199357015433101 1102 100-101 0.20668082055880177 2305 1 0.2027685199808218 2305 2 0.19031188104098007 2305 3 0.16421482376717478 2305 4 0.2738750482887866 2305 5 0.24106979035812515 2305 6 0.25871582705494944 2305 7 0.20710271310528583 2305 8 0.3577663859525657 2305 9 0.2841293853313047 2305 10-11 0.2959547919745873 2305 12-13 0.3130093542952608 2305 14-15 0.31804509575285067 2305 16-17 0.35304704027947764 2305 18-19 0.32561018953356324 2305 20-21 0.2627101166193313 2305 22-23 0.31745218862651114 2305 24-25 0.20778607106197455 2305 26-27 0.2270439940060811 2305 28-29 0.21369294326942168 2305 30-31 0.19414164974993042 2305 32-33 0.1271255398291018 2305 34-35 0.014042985881708603 2305 36-37 0.04655314235642294 2305 38-39 0.07569748758569972 2305 40-41 0.0114002321348039 2305 42-43 -0.025916823432439173 2305 44-45 0.044136861495175594 2305 46-47 0.019185931164827252 2305 48-49 0.008202326937365001 2305 50-51 0.022543595411974593 2305 52-53 0.10552167442439497 2305 54-55 0.16280416060578773 2305 56-57 0.1684394292976421 2305 58-59 0.29790068618750354 2305 60-61 0.10625214155952278 2305 62-63 0.056926301664702805 2305 64-65 0.014824433143083127 2305 66-67 -0.03381192522842014 2305 68-69 0.09458454965533747 2305 70-71 0.08306364888156281 2305 72-73 0.16679892970483223 2305 74-75 0.09333364996864191 2305 76-77 0.017260447386767908 2305 78-79 0.07379300771123809 2305 80-81 -0.011044984710999017 2305 82-83 -0.12072718643044311 2305 84-85 -0.01650134606763487 2305 86-87 -0.03699876531520019 2305 88-89 0.0032741935883109363 2305 90-91 -0.07564392672593456 2305 92-93 -0.0038596874826133387 2305 94-95 0.145563156630363 2305 96-97 0.02931716518099492 2305 98-99 0.1382207647062863 2305 100-101 0.0994031207030126 2203 1 0.16866126206578613 2203 2 0.1795088588956446 2203 3 0.16677358525247143 2203 4 0.11308394172815639 2203 5 0.07535181018212711 2203 6 0.21916535352078625 2203 7 0.07591344948841794 2203 8 0.23629626759108646 2203 9 0.1600943270332067 2203 10-11 0.20099839418823962 2203 12-13 0.16793446263881862 2203 14-15 0.09941596170693856 2203 16-17 0.08852083923267173 2203 18-19 0.14391804411047815 2203 20-21 0.14093460623512044 2203 22-23 0.12102774667306448 2203 24-25 -0.007110616405469727 2203 26-27 0.06510082693192487 2203 28-29 -0.049045478754109695 2203 30-31 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277876.0 34 421594.0 35 713987.0 36 1373318.0 37 1991262.0 38 410654.0 39 469.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.42936606231046 25.280108393008465 23.51935833325573 15.771167211425343 2 30.51149699494662 24.359883740241454 31.62126052374009 13.507358741071837 3 36.63010932445749 20.292156683544977 26.159030567619183 16.918703424378347 4 28.97595213671288 18.990970704715153 33.33915725025676 18.693919908315205 5 32.647254349522264 17.711659990850627 32.0721130897514 17.56897256987571 6 27.283531627834584 18.50585573052824 37.16890290766934 17.04170973396783 7 32.99505835165872 19.267650694213483 29.23344262376459 18.503848330363216 8 28.43014318884789 27.468039076009067 25.91959630494201 18.18222143020104 9 30.341577110795853 25.378746461308154 25.200030212553376 19.07964621534261 10-11 25.184208463576162 28.480798956553265 28.644110806749218 17.690881773121355 12-13 26.1951877726155 25.31140045307868 28.205264840669297 20.288146933636522 14-15 24.583682595828208 25.838235803890008 28.998197677570168 20.579883922711613 16-17 24.641633419130343 26.615339413333327 29.887686383229205 18.855340784307124 18-19 24.040496374957794 26.71390199459132 29.95821667185966 19.28738495859122 20-21 23.5280835555233 27.869390914923304 29.174515621222362 19.428009908331035 22-23 24.796174007090496 27.069472086704394 28.55336486095324 19.58098904525187 24-25 23.076967375295965 28.090685354194672 29.01518880460392 19.817158465905443 26-27 22.50498548740448 28.615683704284155 29.001049351371865 19.878281456939494 28-29 22.61861050954931 28.553450510529267 28.507485960785473 20.320453019135954 30-31 23.26816691436922 27.902727653663284 28.66922175054961 20.15988368141788 32-33 21.952255664070314 27.627237882212818 29.535915855978413 20.884590597738452 34-35 21.109172658614064 27.824624504586637 31.1990652740788 19.8671375627205 36-37 21.48488876938117 28.218098204527724 29.866219636663555 20.430793389427553 38-39 21.17737765504337 27.89229566085402 28.804732717776773 22.125593966325837 40-41 21.928098969217043 27.584568747635664 29.112325938737527 21.375006344409773 42-43 22.83018116737656 27.748575600039565 29.596345362014382 19.82489787056949 44-45 21.66711292863008 28.694841146933403 29.878024752577208 19.760021171859304 46-47 22.313090015478494 28.80246536386118 29.08629473194843 19.798149888711897 48-49 21.241240013296427 28.697307535275495 29.568641882943535 20.49281056848454 50-51 20.825404995426293 28.287482899631577 29.389938160713726 21.497173944228404 52-53 22.77128148865466 27.849729855128707 28.492556166934417 20.88643248928221 54-55 23.274676834300966 27.87007974476452 28.503924669842963 20.351318751091547 56-57 23.59961467326208 27.500418225034295 28.064470088669925 20.835497013033695 58-59 22.833246572587807 27.527895516483987 28.228850309614213 21.410007601313996 60-61 21.292380833229075 27.852328862288665 27.97953644221302 22.87575386226924 62-63 22.35463553405594 27.95528964092977 28.27699632457341 21.413078500440875 64-65 21.54221078729359 28.525230975213482 29.09018591015837 20.842372327334555 66-67 21.604236253077712 28.38136910458951 28.33548812527505 21.678906517057726 68-69 21.586897593638575 27.682839637040747 30.139995461455012 20.590267307865673 70-71 21.301013311584065 29.691745151872407 28.904626682286523 20.102614854257006 72-73 21.278461565475254 31.228547627471904 27.48711556268479 20.005875244368056 74-75 21.851448667263416 30.7822445795415 27.115548386294698 20.250758366900392 76-77 21.91452042670174 29.974449918857555 27.632825304758097 20.478204349682606 78-79 21.724167618113434 29.76328284177452 28.290611081351692 20.22193845876036 80-81 21.182562868407135 30.20595248410004 28.03919884961587 20.572285797876948 82-83 20.388570474510875 30.09586010007842 28.37158775252304 21.14398167288766 84-85 20.54078048568919 29.84900066091213 28.45234091375629 21.157877939642393 86-87 20.38587932014581 29.911484217115486 29.014279512087526 20.688356950651176 88-89 20.502911683142212 30.182202121087094 28.829262096878256 20.485624098892444 90-91 20.97104882291919 29.55452188381118 28.332163204897455 21.14226608837217 92-93 20.61029265829458 30.177906700782497 27.954376010913002 21.257424630009922 94-95 20.317574950014674 30.411770606849164 27.956854141844957 21.31380030129121 96-97 20.697313238952518 30.775347557271004 27.6788752616294 20.848463942147077 98-99 20.95483954997384 30.178576049682455 27.570082382114403 21.2965020182293 100-101 21.05040361281458 30.048983759897972 27.13994838170185 21.760664245585595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1134.0 1 991.5 2 664.5 3 404.5 4 351.5 5 408.5 6 567.5 7 842.5 8 1215.5 9 1645.0 10 2002.5 11 2343.5 12 2487.5 13 2338.5 14 2070.5 15 1802.0 16 1567.5 17 1365.5 18 1295.0 19 1294.0 20 1395.0 21 1673.5 22 2042.5 23 2510.5 24 3146.0 25 4094.5 26 5597.5 27 7821.5 28 10178.5 29 12538.5 30 15742.5 31 20376.5 32 31521.0 33 53524.5 34 88889.0 35 140311.5 36 209886.5 37 295315.0 38 378515.0 39 413329.0 40 416826.5 41 400850.5 42 379078.5 43 392127.5 44 396165.5 45 388834.5 46 394112.5 47 361302.5 48 292751.0 49 243162.0 50 204489.5 51 176374.0 52 155459.5 53 128164.5 54 96721.0 55 68853.5 56 51548.5 57 39860.5 58 32046.5 59 25528.5 60 19721.0 61 15316.0 62 11753.5 63 9152.0 64 7294.5 65 5592.5 66 4138.5 67 3124.5 68 2444.5 69 1786.5 70 1252.0 71 974.0 72 765.0 73 591.5 74 442.0 75 316.0 76 242.0 77 206.5 78 159.0 79 129.5 80 104.0 81 73.5 82 65.5 83 58.5 84 46.0 85 41.0 86 35.5 87 26.5 88 24.5 89 18.0 90 13.0 91 16.0 92 16.0 93 12.0 94 8.0 95 5.5 96 3.0 97 2.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1803910973182394 2 0.18668092774688977 3 0.1565176276370811 4 0.15763306554561024 5 0.16589040450736064 6 0.16255958297494724 7 0.1306301728433008 8 0.1119310956822638 9 0.1117451893641756 10-11 0.09066806055092717 12-13 0.1354482449204197 14-15 0.11385987373242876 16-17 0.10677994145190356 18-19 0.10963825109250946 20-21 0.10152034186932521 22-23 0.10310829166966182 24-25 0.4632398141928319 26-27 0.308860109213765 28-29 0.40274279984830047 30-31 0.2691226337224146 32-33 0.2979381130260839 34-35 0.3170399872096453 36-37 0.29750433161721146 38-39 0.34649064643344923 40-41 0.341378222686024 42-43 0.3840204843975024 44-45 0.3376755885174343 46-47 0.3618821403518339 48-49 0.35728095897915124 50-51 0.37307524992006025 52-53 0.36406653958937013 54-55 0.33931776099388 56-57 0.355081067548441 58-59 0.34738919363754217 60-61 0.3175744678741489 62-63 0.3261571428925536 64-65 0.3278922685280434 66-67 0.3395114134085552 68-69 0.32663740088094806 70-71 0.31477038090965204 72-73 0.3003471490646433 74-75 0.30959598838953073 76-77 0.29850357807693545 78-79 0.3087129333786119 80-81 0.31745827642534374 82-83 0.3440583721051288 84-85 0.34218381673107284 86-87 0.3355763963423552 88-89 0.3486053308017024 90-91 0.3378769870286965 92-93 0.3207658596679961 94-95 0.31333735304105553 96-97 0.29797684350901893 98-99 0.3235157239563839 100-101 0.3268697837785583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6454864.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.51150999667189 #Duplication Level Percentage of deduplicated Percentage of total 1 86.82405097633196 31.700772051738934 2 5.899164106907487 4.307747785227214 3 1.888698404859485 2.0687769206917594 4 1.1726289743162006 1.7125781807253222 5 0.6179307524524362 1.1280792422709054 6 0.4608090991520558 1.0094901618148588 7 0.35276232691248066 0.9015919657859991 8 0.26795223188480616 0.7826672474474115 9 0.22510955031504265 0.7397180637006593 >10 1.8032228996821338 13.193747109388227 >50 0.21540955027528272 5.498832812884632 >100 0.2157938881408173 16.905386275660923 >500 0.040482179196396226 10.216229234235804 >1k 0.015686002099731915 9.139384141361779 >5k 2.990574733136262E-4 0.6949988070656751 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 7543 0.1168576131116008 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 7531 0.11667170679351262 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6884 0.10664825780992442 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6775 0.1049596087539567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011619144880511813 0.0 0.0 2 0.0013942973856614176 0.0 0.0 3 0.0015027427378795277 0.0 0.0 4 0.0016266802832716537 0.0 0.0 5 0.0019984929194480318 0.0 0.0 6 0.002401289941972441 0.0 0.0 7 0.00298999328258504 0.0 0.0 8 0.003392790305109449 0.0 0.0 9 0.004027970225244095 0.0 0.0 10-11 0.006382783587694489 0.0 0.0 12-13 0.008791819626253939 0.0 0.0 14-15 0.011417746369249608 0.0 0.0 16-17 0.013687352669242914 0.0 0.0 18-19 0.01657664669619685 0.0 0.0 20-21 0.01976029239345709 0.0 1.549219317401575E-5 22-23 0.023277020243958665 0.0 1.549219317401575E-5 24-25 0.027328228758963784 0.0 1.549219317401575E-5 26-27 0.03132521459785985 0.0 1.549219317401575E-5 28-29 0.03554683723777914 0.0 1.549219317401575E-5 30-31 0.06330110130902836 0.0 1.549219317401575E-5 32-33 0.07014865069194332 0.0 1.549219317401575E-5 34-35 0.07536177369499962 0.0 1.549219317401575E-5 36-37 0.08121007661819056 0.0 1.549219317401575E-5 38-39 0.08815057916014962 0.0 1.549219317401575E-5 40-41 0.09563330846319923 0.0 1.549219317401575E-5 42-43 0.10437090541334411 0.0 1.549219317401575E-5 44-45 0.11412324101638702 0.0 1.549219317401575E-5 46-47 0.12451850263615159 0.0 1.549219317401575E-5 48-49 0.13668762037434096 0.0 1.549219317401575E-5 50-51 0.15012709795279963 0.0 1.549219317401575E-5 52-53 0.1646665212466134 0.0 1.549219317401575E-5 54-55 0.18002703077865 0.0 1.549219317401575E-5 56-57 0.19836204140009767 0.0 2.3238289761023627E-5 58-59 0.2187575137136894 0.0 3.09843863480315E-5 60-61 0.24092684214570592 0.0 3.09843863480315E-5 62-63 0.2661016560534815 0.0 3.09843863480315E-5 64-65 0.293151025335313 0.0 3.09843863480315E-5 66-67 0.3223538094683327 0.0 3.09843863480315E-5 68-69 0.35587891549690276 0.0 3.09843863480315E-5 70-71 0.3927735735408213 0.0 3.09843863480315E-5 72-73 0.43364972523046186 0.0 3.09843863480315E-5 74-75 0.4793904255767434 0.0 3.09843863480315E-5 76-77 0.5294302095288143 0.0 3.09843863480315E-5 78-79 0.5847838157395725 0.0 3.09843863480315E-5 80-81 0.6474032605489441 0.0 3.09843863480315E-5 82-83 0.7163512662699012 0.0 3.09843863480315E-5 84-85 0.7951135763665973 0.0 3.09843863480315E-5 86-87 0.8802121934714658 0.0 3.09843863480315E-5 88-89 0.9745596499012217 0.0 3.09843863480315E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2375 0.0 75.8429 9 CGGGAGA 3460 0.0 75.09434 4 AGAGCGG 2625 0.0 71.87017 8 AAGCGGC 925 0.0 64.568954 9 TCGGGAA 1205 0.0 61.775368 3 TCGGGAG 4045 0.0 61.4208 3 GAGAGCG 3810 0.0 59.594864 7 CGGAAAG 1175 0.0 58.1064 5 ATTCGGA 360 0.0 56.640793 2 AAGCGTC 500 0.0 52.141994 9 TTGTGTT 67755 0.0 49.394573 1 GATCCGG 620 0.0 48.18915 1 GGTCCGG 420 0.0 47.424248 1 GATCGGG 6795 0.0 46.76122 1 ACCGGAA 530 0.0 43.835194 3 GGATCGG 1260 0.0 43.66042 1 AACCGGA 480 0.0 42.48059 2 GGGAGAG 8700 0.0 42.344902 5 CCGTGTA 1690 0.0 41.75844 38-39 ATCGGGA 7115 0.0 41.38854 2 >>END_MODULE