FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6454864
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA75430.1168576131116008No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT75310.11667170679351262Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG68840.10664825780992442No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC67750.1049596087539567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC23750.075.84299
CGGGAGA34600.075.094344
AGAGCGG26250.071.870178
AAGCGGC9250.064.5689549
TCGGGAA12050.061.7753683
TCGGGAG40450.061.42083
GAGAGCG38100.059.5948647
CGGAAAG11750.058.10645
ATTCGGA3600.056.6407932
AAGCGTC5000.052.1419949
TTGTGTT677550.049.3945731
GATCCGG6200.048.189151
GGTCCGG4200.047.4242481
GATCGGG67950.046.761221
ACCGGAA5300.043.8351943
GGATCGG12600.043.660421
AACCGGA4800.042.480592
GGGAGAG87000.042.3449025
CCGTGTA16900.041.7584438-39
ATCGGGA71150.041.388542
Produced by FastQC (version 0.11.2)