FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_52150223-1.3420000003573b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_52150223-1.3420000003573b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071072
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT65720.21399693657459026Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA24800.084.7433552
GATCGGG26350.081.0227051
GAGCGGC19200.079.273999
AGAGCGG21250.072.518918
TCGGGAG25800.071.877043
CGGGAGA27100.068.779084
GATCCGG4800.067.2113341
AAGCGGC6350.067.204099
GAGAGCG25450.066.8857357
ATTCGGA4650.063.254632
ATCCGGA5250.061.4473572
AAAGCGG8150.058.1794058
TCGGAGA5250.057.816774
TCGGGAA8350.055.0957833
TTCGGAG4250.053.5655333
CGGAAAG10500.052.3955885
GTTCGGA2500.051.236252
GAGCGTC71750.048.044029
AGAGCGT75350.046.440838
TCCGGAG4400.046.349913
Produced by FastQC (version 0.11.2)