##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_50331580-1.342000000357a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3035834 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804231720179693 33.0 33.0 33.0 27.0 33.0 2 31.658112070686343 33.0 33.0 33.0 27.0 33.0 3 31.597442745551962 33.0 33.0 33.0 27.0 33.0 4 35.24898067549148 37.0 37.0 37.0 33.0 37.0 5 35.29250545319672 37.0 37.0 37.0 33.0 37.0 6 35.41399134471779 37.0 37.0 37.0 33.0 37.0 7 35.19914560545801 37.0 37.0 37.0 33.0 37.0 8 35.26952593587133 37.0 37.0 37.0 33.0 37.0 9 35.083964406486 37.0 37.0 37.0 33.0 37.0 10-11 35.01881476391661 37.0 37.0 37.0 33.0 37.0 12-13 34.9793117805519 37.0 37.0 37.0 33.0 37.0 14-15 36.64371157975041 40.0 37.0 40.0 33.0 40.0 16-17 36.60957631411994 40.0 37.0 40.0 33.0 40.0 18-19 36.62812541792469 40.0 37.0 40.0 33.0 40.0 20-21 36.634008480042056 40.0 37.0 40.0 33.0 40.0 22-23 36.801353268986375 40.0 37.0 40.0 33.0 40.0 24-25 36.453236738240626 40.0 37.0 40.0 33.0 40.0 26-27 36.37193057986701 40.0 37.0 40.0 33.0 40.0 28-29 36.213453864737005 40.0 37.0 40.0 33.0 40.0 30-31 36.227156524368596 40.0 37.0 40.0 33.0 40.0 32-33 36.10456484116062 40.0 37.0 40.0 33.0 40.0 34-35 36.16972123640489 40.0 37.0 40.0 33.0 40.0 36-37 35.985734233162944 40.0 37.0 40.0 27.0 40.0 38-39 35.91204360976259 40.0 37.0 40.0 27.0 40.0 40-41 35.95647439879782 38.5 37.0 40.0 27.0 40.0 42-43 35.79740246008181 37.0 37.0 40.0 27.0 40.0 44-45 35.5833663830104 37.0 37.0 40.0 27.0 40.0 46-47 35.50113543757662 37.0 37.0 40.0 27.0 40.0 48-49 35.39743345650652 37.0 37.0 40.0 27.0 40.0 50-51 34.27645994477959 37.0 35.0 38.5 24.5 38.5 52-53 34.716769428104435 37.0 35.0 38.5 27.0 40.0 54-55 35.487572278326155 37.0 37.0 40.0 27.0 40.0 56-57 35.57904977676645 37.0 37.0 40.0 27.0 40.0 58-59 35.31644483855178 37.0 37.0 40.0 27.0 40.0 60-61 35.13866436702402 37.0 37.0 40.0 27.0 40.0 62-63 34.872164617696484 37.0 35.0 40.0 27.0 40.0 64-65 34.704313048737184 37.0 33.0 40.0 27.0 40.0 66-67 34.50523447592984 37.0 33.0 40.0 27.0 40.0 68-69 34.35710598800857 37.0 33.0 38.5 27.0 40.0 70-71 34.02239121111366 37.0 33.0 37.0 27.0 40.0 72-73 33.88325349805029 37.0 33.0 37.0 27.0 40.0 74-75 33.71864683642123 37.0 33.0 37.0 27.0 40.0 76-77 33.44949196826967 37.0 33.0 37.0 24.5 38.5 78-79 33.14884295386375 37.0 33.0 37.0 22.0 37.0 80-81 32.80652812373799 37.0 33.0 37.0 22.0 37.0 82-83 32.51789755302826 37.0 33.0 37.0 22.0 37.0 84-85 32.31329216287847 37.0 33.0 37.0 22.0 37.0 86-87 32.1288721649471 37.0 33.0 37.0 22.0 37.0 88-89 31.94256635903017 35.0 33.0 37.0 22.0 37.0 90-91 31.773168262823333 33.0 33.0 37.0 15.0 37.0 92-93 31.629792175725022 33.0 33.0 37.0 15.0 37.0 94-95 31.479178044649345 33.0 33.0 37.0 15.0 37.0 96-97 31.310737675380143 33.0 33.0 37.0 15.0 37.0 98-99 31.16235406810781 33.0 33.0 37.0 15.0 37.0 100-101 29.89106947217799 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.184590608926392 2306 2 0.09262720072874231 2306 3 0.0918038856596155 2306 4 0.17137249097840623 2306 5 0.17132043429413812 2306 6 0.22716534274859868 2306 7 0.3972542458916948 2306 8 0.11022828617053904 2306 9 0.13392156580334103 2306 10-11 0.2427112102332174 2306 12-13 0.28918383788266766 2306 14-15 0.6021933984388284 2306 16-17 0.46524367847802495 2306 18-19 0.49089021439798586 2306 20-21 0.3491198015784178 2306 22-23 0.4003700965720043 2306 24-25 0.4559981542185767 2306 26-27 0.4360933388996884 2306 28-29 0.34385298261111785 2306 30-31 0.2918873985099637 2306 32-33 0.3241278399116396 2306 34-35 0.2175418851498705 2306 36-37 0.1955658135640732 2306 38-39 0.3290868505708744 2306 40-41 0.29858489977660696 2306 42-43 0.39703649475612934 2306 44-45 0.36271621218499206 2306 46-47 0.2649692948169644 2306 48-49 0.35363836274145655 2306 50-51 0.27993164853712216 2306 52-53 0.27412280582601767 2306 54-55 0.3339673533494647 2306 56-57 0.3796883578702577 2306 58-59 0.3774187998805658 2306 60-61 0.41309554293907524 2306 62-63 0.24728453335622902 2306 64-65 0.23666782101906136 2306 66-67 0.26507123640111985 2306 68-69 0.15437292170408767 2306 70-71 0.20704582026931462 2306 72-73 0.14453773346856735 2306 74-75 0.1693395617632305 2306 76-77 0.24414045576808263 2306 78-79 0.26922183649531206 2306 80-81 0.43884680251972696 2306 82-83 0.3958463073270124 2306 84-85 0.30765690685927893 2306 86-87 0.2471765246499018 2306 88-89 0.15002606790672246 2306 90-91 0.17625249214640704 2306 92-93 0.1867673336348723 2306 94-95 0.17993813909105327 2306 96-97 0.1829369225357489 2306 98-99 0.09759766958504557 2306 100-101 -0.01245169700508697 2307 1 0.21959848840597118 2307 2 0.19336538757198696 2307 3 0.21781731963286077 2307 4 0.2732769897382923 2307 5 0.20587633926377436 2307 6 0.261217717180557 2307 7 0.4589859059327779 2307 8 0.25553952322687934 2307 9 0.28628624030054084 2307 10-11 0.33061500133888444 2307 12-13 0.38118900646992415 2307 14-15 0.5496653486181415 2307 16-17 0.529872596330371 2307 18-19 0.5732379842465392 2307 20-21 0.5038036380572137 2307 22-23 0.31333903444718203 2307 24-25 0.31670293275583816 2307 26-27 0.3290941498569069 2307 28-29 0.306857809210058 2307 30-31 0.3160776776782157 2307 32-33 0.3471706155659646 2307 34-35 0.2282693578867594 2307 36-37 0.20540967326981274 2307 38-39 0.26948216309683204 2307 40-41 0.2632732216298095 2307 42-43 0.2096040785026858 2307 44-45 0.23928649899499987 2307 46-47 0.2372800798987882 2307 48-49 0.2475752974988339 2307 50-51 0.13889688850846937 2307 52-53 0.1316196537477694 2307 54-55 0.2695454234011265 2307 56-57 0.27104252658563865 2307 58-59 0.4319622130156233 2307 60-61 0.31381581883638177 2307 62-63 0.4387508268845508 2307 64-65 0.22116205925106414 2307 66-67 0.32357606194984356 2307 68-69 0.24864674646056528 2307 70-71 0.2430467261278153 2307 72-73 0.22818376047514022 2307 74-75 0.0909284765298537 2307 76-77 0.2144640424025468 2307 78-79 0.3585555435780208 2307 80-81 0.29198716609665354 2307 82-83 0.11518455324964094 2307 84-85 0.23704538935724173 2307 86-87 0.20954339314250348 2307 88-89 0.25080645431341964 2307 90-91 0.376996292868224 2307 92-93 0.3391736348507699 2307 94-95 0.31757086960712044 2307 96-97 0.28492171503514996 2307 98-99 0.3514108584189266 2307 100-101 0.108459886125857 2201 1 0.35924789753874364 2201 2 0.2748285131388286 2201 3 0.3407999565140898 2201 4 0.46541931769301215 2201 5 0.3748173326713413 2201 6 0.4483239890419739 2201 7 0.5052747145809633 2201 8 0.3758823099450268 2201 9 0.5085002250000912 2201 10-11 0.548712802496766 2201 12-13 0.49601452689348946 2201 14-15 0.7321185806539177 2201 16-17 0.6853682120183251 2201 18-19 0.7759913352964389 2201 20-21 0.7202884733011459 2201 22-23 0.6363214829878245 2201 24-25 0.7133655033000892 2201 26-27 0.6731139852027184 2201 28-29 0.669058503912872 2201 30-31 0.7383347923723278 2201 32-33 0.6567046900594633 2201 34-35 0.649314500899763 2201 36-37 0.6742818538754207 2201 38-39 0.5898110639771161 2201 40-41 0.5030572429170945 2201 42-43 0.5518889545994057 2201 44-45 0.6292474833861874 2201 46-47 0.5308427869557235 2201 48-49 0.6039145762879272 2201 50-51 0.6352976142703639 2201 52-53 0.6106416798370518 2201 54-55 0.6313714915533666 2201 56-57 0.7391491883120693 2201 58-59 0.7874102983880178 2201 60-61 0.8081634609748889 2201 62-63 0.7747440144342761 2201 64-65 0.7129628229892688 2201 66-67 0.6603593382644917 2201 68-69 0.62927348572169 2201 70-71 0.6926292349716903 2201 72-73 0.6583350852885204 2201 74-75 0.7465237415105008 2201 76-77 0.735503740660377 2201 78-79 0.7512983184478372 2201 80-81 0.7497390335363789 2201 82-83 0.6604610312395636 2201 84-85 0.690142419174812 2201 86-87 0.7086135899053403 2201 88-89 0.6458575999121621 2201 90-91 0.6502302873854582 2201 92-93 0.6541738668521369 2201 94-95 0.7713483226020195 2201 96-97 0.6291561730414479 2201 98-99 0.6309040955364722 2201 100-101 0.5810707130043902 1101 1 0.1768971321758599 1101 2 0.06577610704163561 1101 3 0.019273538392592826 1101 4 0.11576748240425161 1101 5 0.14563243425002526 1101 6 0.18019359058852302 1101 7 0.31908987301800096 1101 8 0.26930503449844423 1101 9 0.2770677220459348 1101 10-11 0.20961421752814147 1101 12-13 0.1816399780521678 1101 14-15 0.32508237486128166 1101 16-17 0.23785542506904278 1101 18-19 0.24958171164929865 1101 20-21 0.272113008422302 1101 22-23 0.2512413312501849 1101 24-25 0.18679496124221373 1101 26-27 0.17912304792984202 1101 28-29 0.20363618540226724 1101 30-31 0.1295730282553862 1101 32-33 0.2039008316021551 1101 34-35 0.12474605271255257 1101 36-37 0.004247334056572072 1101 38-39 0.17790388956534997 1101 40-41 0.02119413063915232 1101 42-43 0.10346295760053437 1101 44-45 0.0502013935104344 1101 46-47 0.03167812578206508 1101 48-49 -0.0917754987156556 1101 50-51 0.015681080602803377 1101 52-53 0.02054969599965517 1101 54-55 0.20083439951205406 1101 56-57 0.10564600609835395 1101 58-59 -0.15028298911254012 1101 60-61 0.15706933938700018 1101 62-63 0.22466262046521734 1101 64-65 0.1282781767223895 1101 66-67 0.1639852170968723 1101 68-69 0.2097771889309641 1101 70-71 0.28039558854659674 1101 72-73 0.2292281816955608 1101 74-75 0.20696322877176243 1101 76-77 0.043249887888904937 1101 78-79 0.11745638096235922 1101 80-81 0.285998980658114 1101 82-83 0.1588376325438432 1101 84-85 0.12917079643740692 1101 86-87 0.2270610354820093 1101 88-89 0.10761881213225877 1101 90-91 0.06827036140020226 1101 92-93 0.17539331166739558 1101 94-95 0.1489979527755132 1101 96-97 0.18120072526955866 1101 98-99 0.1807001934465582 1101 100-101 0.035531343538806937 2304 1 0.10486611894737408 2304 2 0.10257566154146147 2304 3 0.051255894719922424 2304 4 0.12221691085346009 2304 5 0.20262027969468477 2304 6 0.22486306040235604 2304 7 0.187895702745017 2304 8 0.22720777075232945 2304 9 0.17735164877275622 2304 10-11 0.2557731436861488 2304 12-13 0.20303139534794923 2304 14-15 0.18433820697734404 2304 16-17 0.2562321650908004 2304 18-19 0.2722607251326181 2304 20-21 0.2851714194565105 2304 22-23 0.32212048307064123 2304 24-25 0.19295848631354318 2304 26-27 0.16032296328936013 2304 28-29 0.11208360989704147 2304 30-31 0.04219858114885966 2304 32-33 -0.014006318698413622 2304 34-35 -0.06198852827154866 2304 36-37 -0.03259950364302 2304 38-39 -0.0769834868299597 2304 40-41 -0.02799660850889296 2304 42-43 0.07883267667149596 2304 44-45 0.06496171532629091 2304 46-47 0.011542664036376493 2304 48-49 -0.021752848285395032 2304 50-51 -0.12232144382235077 2304 52-53 -0.15278191096361837 2304 54-55 0.0759146742978345 2304 56-57 0.07304953699440375 2304 58-59 0.09637021357411157 2304 60-61 0.21224762478015435 2304 62-63 0.10212419095572756 2304 64-65 0.050427419281085406 2304 66-67 0.1777416419238378 2304 68-69 0.23618666912008734 2304 70-71 0.04463778603651036 2304 72-73 0.07557783334991797 2304 74-75 0.05227257892853032 2304 76-77 0.12086060310098645 2304 78-79 0.08100963734566591 2304 80-81 0.11813459143476024 2304 82-83 0.06839788314338335 2304 84-85 0.12139814942007376 2304 86-87 0.1703968488724925 2304 88-89 0.2709615765086255 2304 90-91 -0.018937919032829598 2304 92-93 0.13494548501656212 2304 94-95 0.14528455068316148 2304 96-97 0.17132657110556337 2304 98-99 0.012817459763741823 2304 100-101 -0.06271347564454643 2202 1 0.06172278229406558 2202 2 -0.0771349778020749 2202 3 -0.030600049734541557 2202 4 0.0025073694544204272 2202 5 -0.06474318960077596 2202 6 0.14584896227226096 2202 7 0.1457677033305842 2202 8 0.03196592233663864 2202 9 0.10354690512972553 2202 10-11 0.09285889837919825 2202 12-13 -0.014630871698294357 2202 14-15 0.1414346535584201 2202 16-17 0.10567293229258468 2202 18-19 0.10668540221929845 2202 20-21 0.11096906305849785 2202 22-23 0.29425256056460825 2202 24-25 0.1945495908718442 2202 26-27 0.2724248603805961 2202 28-29 0.1854862313702199 2202 30-31 0.09830086513481717 2202 32-33 0.0562189298422382 2202 34-35 0.05427307530251824 2202 36-37 -0.11248418025915896 2202 38-39 -0.03836466178741915 2202 40-41 0.1605525493415385 2202 42-43 0.12894972539670135 2202 44-45 0.09509238379149565 2202 46-47 0.09380791958295731 2202 48-49 0.10973816033347106 2202 50-51 0.08045485260175411 2202 52-53 0.0442010290703152 2202 54-55 0.19937245441091989 2202 56-57 0.1962283215698548 2202 58-59 0.19787008417350904 2202 60-61 0.34519811532606326 2202 62-63 0.28990099713976036 2202 64-65 0.1572150036202018 2202 66-67 0.19846364499284874 2202 68-69 0.20333839197798653 2202 70-71 0.23935523525004498 2202 72-73 0.28548639025873257 2202 74-75 0.29129718595589793 2202 76-77 0.24071836700631621 2202 78-79 0.29082944374145114 2202 80-81 0.16518288530743774 2202 82-83 0.2982600780389859 2202 84-85 0.18112443447260063 2202 86-87 0.24881701131599243 2202 88-89 0.2924920719168682 2202 90-91 0.2744514094695454 2202 92-93 0.23754362687622077 2202 94-95 0.2002669966420747 2202 96-97 0.12963781484139858 2202 98-99 0.06336617059391614 2202 100-101 0.21329434590287377 1102 1 0.005145711612243531 1102 2 -0.050512275904775805 1102 3 -0.030230490803617727 1102 4 0.058733506191224194 1102 5 -0.06886200720825286 1102 6 -0.039646094754502315 1102 7 0.029685763195210768 1102 8 0.009721562521512794 1102 9 0.0042205904752918855 1102 10-11 0.07008703485133339 1102 12-13 0.11734920936940796 1102 14-15 0.05985464208934843 1102 16-17 0.005946548039510446 1102 18-19 0.07812410886106846 1102 20-21 0.14704688609172223 1102 22-23 0.13161403723190546 1102 24-25 -0.1484128601763075 1102 26-27 0.08998907982213211 1102 28-29 0.025692061971078317 1102 30-31 -0.12575048591185833 1102 32-33 -0.07996659952397778 1102 34-35 -0.11492193320125921 1102 36-37 -0.17841920356489993 1102 38-39 -0.08218496498258077 1102 40-41 0.08334965462662325 1102 42-43 -0.16349355416923572 1102 44-45 -0.25760125479555285 1102 46-47 -0.14058054370865847 1102 48-49 -0.15030389292419244 1102 50-51 -0.18856040028144605 1102 52-53 -0.16499689571414677 1102 54-55 -0.026579625460854572 1102 56-57 0.08864550164896201 1102 58-59 0.011793241402706656 1102 60-61 0.040597253675393574 1102 62-63 0.07986911410467457 1102 64-65 0.06674548242938982 1102 66-67 0.03213075767892093 1102 68-69 0.07507693638632418 1102 70-71 0.1360973331096389 1102 72-73 0.06084553390149239 1102 74-75 -0.05580491327606296 1102 76-77 -0.10967986460028101 1102 78-79 -0.04245487647106927 1102 80-81 0.08611504134561443 1102 82-83 0.06561501145466053 1102 84-85 0.05109152216198254 1102 86-87 0.07558650751012408 1102 88-89 0.16832284431464473 1102 90-91 0.15265551970986024 1102 92-93 0.07833026295869772 1102 94-95 0.17358719445902793 1102 96-97 0.0652893462563604 1102 98-99 0.09389423795754936 1102 100-101 0.01726318336066157 2305 1 0.1838101695851293 2305 2 0.2699319169554002 2305 3 0.2086885920559709 2305 4 0.20403094477784833 2305 5 0.22933179525256975 2305 6 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1213 84-85 -1.332539183489022 1213 86-87 -1.2429589088633648 1213 88-89 -1.3102340692441103 1213 90-91 -1.2731034400006394 1213 92-93 -1.3367372548726273 1213 94-95 -1.3913777698300898 1213 96-97 -1.451658552943293 1213 98-99 -1.2799128205618686 1213 100-101 -1.2275409794820469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1183.0 3 217.0 4 245.0 5 318.0 6 734.0 7 2843.0 8 9276.0 9 19543.0 10 27714.0 11 24234.0 12 16320.0 13 12239.0 14 9514.0 15 8404.0 16 8062.0 17 8280.0 18 8807.0 19 9616.0 20 10812.0 21 11755.0 22 12975.0 23 14703.0 24 16497.0 25 19315.0 26 22446.0 27 26605.0 28 32090.0 29 39301.0 30 47557.0 31 60713.0 32 81714.0 33 114779.0 34 170480.0 35 280883.0 36 521871.0 37 938988.0 38 441830.0 39 2971.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.591664389790616 24.197615681802283 24.335812158386098 15.874907770020998 2 29.9097840929866 23.93173449634787 32.99002140392029 13.168460006745239 3 37.86999739374565 20.481563224761395 26.011342155009455 15.637097226483505 4 28.964103626587452 20.26066983953754 34.275484194919756 16.499742338955247 5 32.43042429213433 18.52719334509439 33.06604260704799 15.976339755723288 6 26.42391777366246 19.396321484576955 38.514083533518026 15.665677208242561 7 33.21368437543805 19.7001904730493 29.897441394493597 17.188683757019056 8 28.621627771237495 29.303608585683712 25.821024966281836 16.253738676796956 9 32.45748053956638 26.563702107382355 23.614455050149466 17.364362302901792 10-11 27.69419557526544 28.966652528256077 27.323701080920777 16.015450815557703 12-13 28.729722682674176 26.217273987723566 26.81739071923466 18.2356126103676 14-15 26.748199139913808 26.66204541958181 28.064941272842486 18.524814167661898 16-17 25.989652460508374 27.5028399676186 28.589793263547346 17.91771430832568 18-19 25.084204174100005 28.126848716080076 28.928560829148132 17.860386280671786 20-21 24.312559980519367 28.77473967156271 28.685579829959917 18.22712051795801 22-23 25.148132980386105 28.38092713275221 28.111996117052918 18.358943769808768 24-25 23.64130165039041 29.45467762823962 28.432853747972498 18.47116697339747 26-27 23.306046735065706 29.661319202649615 28.210018867086077 18.822615195198598 28-29 23.282081196863132 29.71182102741378 28.072420656080922 18.933677119642166 30-31 23.76211448361433 28.58493848366321 28.565601876261898 19.087345156460565 32-33 22.540686429157795 29.555517382061797 28.862250030144214 19.041546158636194 34-35 23.772218746473886 28.81679707744175 28.655093553616663 18.755890622467703 36-37 22.688897132835415 29.224346304775768 29.084428620178077 19.00232794221074 38-39 22.603532381874818 30.172652984802696 28.231771474125022 18.992043159197465 40-41 23.997883532135013 28.418340165695245 28.113924402041647 19.4698519001281 42-43 23.787814414071864 28.552343795204127 28.25908628770282 19.400755503021188 44-45 22.383613764540893 29.092547668788733 29.17551456910997 19.3483239975604 46-47 22.81950143174127 29.046208261539668 28.481081594790364 19.6532087119287 48-49 21.634441770169268 29.478834763785 28.901756554209936 19.984966911835798 50-51 21.25271048021494 28.56028746049782 29.610477669345713 20.57652438994153 52-53 23.03474646040568 28.648247768379186 28.18712244010486 20.129883331110264 54-55 23.165004017886577 28.56764450387322 28.593339809762202 19.674011668478006 56-57 23.499623433928893 28.313157534188843 28.165803437634228 20.021415594248033 58-59 22.828482804322427 28.71606268477648 28.389583605316602 20.065870905584486 60-61 21.615379900614425 28.500835372132556 28.47286572869512 21.410918998557904 62-63 22.539399512861326 28.79469692288425 28.097826248586117 20.568077315668308 64-65 21.40648454041747 29.18722257883036 29.20777642569594 20.19851645505622 66-67 21.311370032107156 28.802467442548345 28.539413478396657 21.34674904694784 68-69 21.133627065672094 28.633384601836383 30.07503243728283 20.157955895208698 70-71 20.864441052276828 29.814130140290363 29.233177381731196 20.088251425701618 72-73 20.768823284849383 31.17947364326347 27.92840010234503 20.123302969542113 74-75 20.7563171802325 31.028454320250198 28.25187511604872 19.963353383468583 76-77 21.17005533898203 30.569126156790865 28.061602793856306 20.1992157103708 78-79 20.97240703159394 30.725567788077544 28.370943589159058 19.93108159116946 80-81 20.60438961337621 30.595243759474616 28.550593621427733 20.249773005721444 82-83 20.583416784820752 30.660539179233965 28.18683879155636 20.569205244388918 84-85 20.52211669753199 30.32716032348028 28.663390706427883 20.487332272559847 86-87 20.782591381778918 29.939630978140087 28.668948577688656 20.60882906239234 88-89 20.893156732016294 30.43141924898829 28.4310536982759 20.244370320719522 90-91 21.104743120885104 29.792560753865466 28.38443331533317 20.71826280991626 92-93 20.91031859399529 30.144122157381513 28.14915521490779 20.796404033715405 94-95 20.54772365520255 30.21571110323653 28.56593210076926 20.670633140791658 96-97 20.935042239516104 30.13249556297194 28.160183939486288 20.772278258025672 98-99 21.057891552510284 30.0475228915806 28.015970493643955 20.878615062265165 100-101 21.12886098607883 29.733989796176317 27.988395418242273 21.148753799502582 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 587.0 1 517.5 2 366.0 3 263.5 4 239.0 5 266.0 6 357.0 7 484.5 8 663.5 9 848.5 10 1030.5 11 1199.0 12 1288.5 13 1298.5 14 1236.0 15 1086.0 16 983.0 17 1032.5 18 1117.0 19 1342.0 20 1727.5 21 2254.0 22 3071.5 23 4227.5 24 5771.0 25 7938.5 26 10801.0 27 14239.5 28 18425.5 29 23362.5 30 29223.0 31 36045.5 32 44428.5 33 54856.0 34 67570.0 35 83969.5 36 103962.5 37 127058.5 38 149695.0 39 164703.5 40 170508.5 41 166330.5 42 157527.5 43 153722.0 44 150066.0 45 146463.5 46 146323.0 47 138988.0 48 123097.5 49 108837.5 50 97924.0 51 88200.0 52 77896.5 53 66663.5 54 55762.5 55 45601.0 56 36662.5 57 29268.5 58 23543.5 59 18881.5 60 15058.0 61 11626.5 62 8811.5 63 6828.0 64 5152.0 65 3855.0 66 2961.0 67 2248.0 68 1689.0 69 1279.5 70 987.5 71 770.5 72 597.0 73 447.0 74 350.5 75 283.5 76 214.0 77 181.5 78 150.0 79 122.5 80 102.5 81 83.0 82 74.0 83 70.0 84 59.5 85 51.5 86 48.5 87 43.0 88 39.5 89 28.5 90 27.5 91 26.5 92 16.0 93 10.5 94 10.0 95 7.5 96 6.0 97 4.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1767553825406791 2 0.18275044024146248 3 0.15363158855194323 4 0.1554762216906458 5 0.16229477632835 6 0.16084542171936936 7 0.12876198105693526 8 0.11015095028252533 9 0.11196264354375107 10-11 0.0897776360631049 12-13 0.13474056881898022 14-15 0.11408726564100671 16-17 0.10636286437268967 18-19 0.1094921527329887 20-21 0.1014054128124265 22-23 0.10165246189350273 24-25 0.44929992878398495 26-27 0.3008893108121195 28-29 0.3907492965689165 30-31 0.26017562225075547 32-33 0.2872027917204959 34-35 0.3065879096156114 36-37 0.2864781144160056 38-39 0.3359373404474685 40-41 0.33065049011243697 42-43 0.3741640682593317 44-45 0.3270435735287239 46-47 0.3515179024940099 48-49 0.34611576258780946 50-51 0.36237159212262593 52-53 0.3535931147750503 54-55 0.32905290605481063 56-57 0.34483110736621303 58-59 0.33661260793574355 60-61 0.3076090458173932 62-63 0.3159263648802932 64-65 0.317425129305489 66-67 0.3303540312151455 68-69 0.31653575261361455 70-71 0.3048256261706009 72-73 0.29048030952944065 74-75 0.2993576065094468 76-77 0.28866861626821494 78-79 0.2986493991436949 80-81 0.3071972973489328 82-83 0.33348331957544447 84-85 0.3311939980908047 86-87 0.3248366017377762 88-89 0.33802902266724727 90-91 0.32729062260980013 92-93 0.3110512630137221 94-95 0.3035903807652197 96-97 0.28827333773849295 98-99 0.313027655662332 100-101 0.3167333918784756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3035834.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.21950277448451 #Duplication Level Percentage of deduplicated Percentage of total 1 94.49504640149549 75.8034563697981 2 3.4588488741773045 5.549342737171778 3 0.8239246784107174 1.982844840772044 4 0.2864723950927967 0.9192269237183932 5 0.18971020002927175 0.7609228958798089 6 0.16341237588662003 0.7865315724493092 7 0.09423529498958493 0.5291655955509961 8 0.0652858106520736 0.4189756214990791 9 0.06152849450075812 0.44422067117820785 >10 0.2903491578154698 4.404785941713576 >50 0.0343016653280093 1.9345313784598774 >100 0.03504250614308536 5.313403371836315 >500 0.001543907498706368 0.8356442042771328 >1k 2.9823798019477124E-4 0.3169478756954089 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013505349765501012 0.0 0.0 2 0.001383474854026933 0.0 0.0 3 0.0016799337513184185 0.0 0.0 4 0.0019105128936562406 0.0 0.0 5 0.0020752122810403996 0.0 3.293987747683174E-5 6 0.00227285154590139 0.0 3.293987747683174E-5 7 0.0028328294630075293 0.0 3.293987747683174E-5 8 0.0033269276251600053 0.0 3.293987747683174E-5 9 0.003722206154881986 0.0 6.587975495366348E-5 10-11 0.005204500641339415 0.0 8.234969369207934E-5 12-13 0.006555035617889516 0.0 9.881963243049521E-5 14-15 0.00805380004308536 0.0 9.881963243049521E-5 16-17 0.009223165693512886 0.0 9.881963243049521E-5 18-19 0.010672520302493483 0.0 1.3175950990732696E-4 20-21 0.012270104360119823 0.0 1.646993873841587E-4 22-23 0.014378256518637052 0.0 1.646993873841587E-4 24-25 0.016436998860939037 0.0 1.646993873841587E-4 26-27 0.01838045163207211 0.0 1.8116932612257454E-4 28-29 0.020455663913112508 0.0 1.9763926486099042E-4 30-31 0.033598675026368374 0.0 1.9763926486099042E-4 32-33 0.03694207259026679 0.0 2.3057914233782215E-4 34-35 0.039264333952383434 0.0 2.3057914233782215E-4 36-37 0.04194893396674522 0.0 2.3057914233782215E-4 38-39 0.04507822232704423 0.0 2.3057914233782215E-4 40-41 0.04855337940084998 0.0 2.3057914233782215E-4 42-43 0.05275321377914603 0.0 2.47049081076238E-4 44-45 0.05751302607454821 0.0 2.635190198146539E-4 46-47 0.062486947573549806 0.0 2.964588972914856E-4 48-49 0.06779026784731972 0.0 2.964588972914856E-4 50-51 0.07376885560936468 0.0 3.623386522451491E-4 52-53 0.08057094030833042 0.0 3.623386522451491E-4 54-55 0.08847651090277003 0.0 3.623386522451491E-4 56-57 0.09888551218544889 0.0 3.623386522451491E-4 58-59 0.10987096132397225 0.0 3.9527852972198084E-4 60-61 0.12095523009492615 0.0 3.9527852972198084E-4 62-63 0.13332415408747644 0.0 3.9527852972198084E-4 64-65 0.1477024106061135 0.0 4.282184071988126E-4 66-67 0.16381001069228424 0.0 5.270380396293078E-4 68-69 0.1817622439171575 0.0 5.270380396293078E-4 70-71 0.20231672746270052 0.0 5.270380396293078E-4 72-73 0.22609931900097305 0.0 5.435079783677237E-4 74-75 0.2526653301860378 0.0 5.929177945829712E-4 76-77 0.28259120887373945 0.0 5.929177945829712E-4 78-79 0.3151193378821108 0.0 5.929177945829712E-4 80-81 0.35227551967597703 0.0 6.25857672059803E-4 82-83 0.3933515468895862 0.0 6.25857672059803E-4 84-85 0.44223432506520444 0.0 6.423276107982189E-4 86-87 0.4980838873271727 0.0 6.752674882750506E-4 88-89 0.5584791526809436 0.0 6.917374270134665E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1705 0.0 81.506645 2 GAGCGGC 1060 0.0 80.067024 9 AGAGCGG 1235 0.0 68.7215 8 GATCGGG 2100 0.0 67.31268 1 TCGGGAG 1970 0.0 63.55109 3 CGGGAGA 1860 0.0 62.720207 4 GAGAGCG 1735 0.0 60.394768 7 GATCCGG 365 0.0 59.781242 1 TTGTGTT 29485 0.0 58.350746 1 TCGGAGA 455 0.0 51.070488 4 AAGCGGC 390 0.0 51.061222 9 TTCGGAG 345 0.0 50.859013 3 GTTCGGA 200 0.0 49.80112 2 CGCGTCA 35 7.929884E-8 47.499004 60-61 GAGCGTC 3710 0.0 46.77499 9 CCGGAGA 530 0.0 46.52783 4 AGAGCGT 3875 0.0 45.884506 8 GGATCGG 510 0.0 45.57492 1 AAGCGTC 210 0.0 45.15618 9 AGCGGCG 1105 0.0 43.76676 10-11 >>END_MODULE