##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2395603 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07547160360043 33.0 33.0 33.0 33.0 33.0 2 32.02761851608969 33.0 33.0 33.0 33.0 33.0 3 32.000345215797445 33.0 33.0 33.0 33.0 33.0 4 35.74975903770366 37.0 37.0 37.0 33.0 37.0 5 35.775262428707926 37.0 37.0 37.0 33.0 37.0 6 35.77420215286089 37.0 37.0 37.0 33.0 37.0 7 35.69179659567967 37.0 37.0 37.0 33.0 37.0 8 35.654460275763554 37.0 37.0 37.0 33.0 37.0 9 35.60090799685925 37.0 37.0 37.0 33.0 37.0 10-11 35.51778487503981 37.0 37.0 37.0 33.0 37.0 12-13 35.493007606018196 37.0 37.0 37.0 33.0 37.0 14-15 37.19622742165542 40.0 37.0 40.0 33.0 40.0 16-17 37.181987583084506 40.0 37.0 40.0 33.0 40.0 18-19 37.232626190566634 40.0 37.0 40.0 33.0 40.0 20-21 37.25978511464545 40.0 37.0 40.0 33.0 40.0 22-23 37.24898261523299 40.0 37.0 40.0 33.0 40.0 24-25 37.02697358452131 40.0 37.0 40.0 33.0 40.0 26-27 36.96615006743605 40.0 37.0 40.0 33.0 40.0 28-29 36.900776965131534 40.0 37.0 40.0 33.0 40.0 30-31 36.94492409635487 40.0 37.0 40.0 33.0 40.0 32-33 36.86839305177027 40.0 37.0 40.0 33.0 40.0 34-35 36.82752359218118 40.0 37.0 40.0 33.0 40.0 36-37 36.74774033927992 40.0 37.0 40.0 33.0 40.0 38-39 36.6857818261206 40.0 37.0 40.0 33.0 40.0 40-41 36.56897887504733 40.0 37.0 40.0 33.0 40.0 42-43 36.48553015670794 40.0 37.0 40.0 33.0 40.0 44-45 36.37943661783693 40.0 37.0 40.0 33.0 40.0 46-47 36.33193208557511 40.0 37.0 40.0 33.0 40.0 48-49 36.239729621310374 37.0 37.0 40.0 33.0 40.0 50-51 35.071370757174705 37.0 35.0 38.5 30.0 38.5 52-53 35.50015737165131 37.0 37.0 38.5 30.0 40.0 54-55 36.313938912248815 37.0 37.0 40.0 33.0 40.0 56-57 36.33766321047352 37.0 37.0 40.0 33.0 40.0 58-59 36.14872309810933 37.0 37.0 40.0 33.0 40.0 60-61 35.9436993525221 37.0 37.0 40.0 30.0 40.0 62-63 35.724316382973306 37.0 37.0 40.0 27.0 40.0 64-65 35.54540881773816 37.0 37.0 40.0 27.0 40.0 66-67 35.307675770985426 37.0 37.0 40.0 27.0 40.0 68-69 35.09075209874091 37.0 35.0 38.5 27.0 40.0 70-71 34.80108181530913 37.0 33.0 37.0 27.0 40.0 72-73 34.55021721044764 37.0 33.0 37.0 27.0 40.0 74-75 34.32922316427221 37.0 33.0 37.0 27.0 40.0 76-77 34.09610565690559 37.0 33.0 37.0 27.0 37.0 78-79 33.872981249397334 37.0 33.0 37.0 27.0 37.0 80-81 33.631364003134074 37.0 33.0 37.0 27.0 37.0 82-83 33.426696952708774 37.0 33.0 37.0 27.0 37.0 84-85 33.25503224031695 37.0 33.0 37.0 27.0 37.0 86-87 33.083304704494026 37.0 33.0 37.0 27.0 37.0 88-89 32.93909195304898 37.0 33.0 37.0 27.0 37.0 90-91 32.79475898135041 37.0 33.0 37.0 27.0 37.0 92-93 32.66352626040291 37.0 33.0 37.0 27.0 37.0 94-95 32.5341102010642 33.0 33.0 37.0 24.5 37.0 96-97 32.37676463921609 33.0 33.0 37.0 22.0 37.0 98-99 32.24294593052355 33.0 33.0 37.0 22.0 37.0 100-101 31.050916825534117 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3302320859005121 2306 2 0.26856192307636917 2306 3 0.24078787029012716 2306 4 0.27526298953055317 2306 5 0.22212092129836236 2306 6 0.302076244059414 2306 7 0.3050708523453949 2306 8 0.3072063159445335 2306 9 0.36085396239635514 2306 10-11 0.28457412061560916 2306 12-13 0.4127389922551856 2306 14-15 0.6418956930126001 2306 16-17 0.640944703007932 2306 18-19 0.6452907605627303 2306 20-21 0.6089503286454203 2306 22-23 0.49248215008956464 2306 24-25 0.39544590205893826 2306 26-27 0.6187342621678837 2306 28-29 0.4330864641543499 2306 30-31 0.34397745315438044 2306 32-33 0.43977523369514415 2306 34-35 0.31412846106419323 2306 36-37 0.39942293699076004 2306 38-39 0.4028137348803753 2306 40-41 0.3563410427123017 2306 42-43 0.2956796081705093 2306 44-45 0.3497603441570334 2306 46-47 0.3142746618314476 2306 48-49 0.4484054640672923 2306 50-51 0.48770997417065587 2306 52-53 0.28267286697901994 2306 54-55 0.4686646283074438 2306 56-57 0.494842581252513 2306 58-59 0.6057431645351343 2306 60-61 0.32250575031607553 2306 62-63 0.3956955148939798 2306 64-65 0.38246658656326105 2306 66-67 0.4336263928083284 2306 68-69 0.4332562528135071 2306 70-71 0.49862745966112243 2306 72-73 0.3290501895878819 2306 74-75 0.2911056103190717 2306 76-77 0.40755739844429684 2306 78-79 0.23810495618636196 2306 80-81 0.4036973689868404 2306 82-83 0.43479307528854605 2306 84-85 0.25780665830236416 2306 86-87 0.31340666621637325 2306 88-89 0.4729238352809588 2306 90-91 0.31972114016173947 2306 92-93 0.24211675075857642 2306 94-95 0.40968681099712256 2306 96-97 0.4378989444085235 2306 98-99 0.2927549340101834 2306 100-101 0.4177507221150272 2307 1 0.16226986379082575 2307 2 0.12685600994188917 2307 3 0.1659705984682489 2307 4 0.16909496400769086 2307 5 0.16363524414909136 2307 6 0.1556767909779353 2307 7 0.16648271237477275 2307 8 0.2093956780417301 2307 9 0.2205001533789499 2307 10-11 0.24000870139580854 2307 12-13 0.22055832346160997 2307 14-15 0.41552206663897095 2307 16-17 0.42886396500553303 2307 18-19 0.44101331757561013 2307 20-21 0.3965062443119365 2307 22-23 0.40983077688519387 2307 24-25 0.23262918728369897 2307 26-27 0.28977965302893693 2307 28-29 0.4990817208178058 2307 30-31 0.3942458483536484 2307 32-33 0.2537651224546167 2307 34-35 0.224795060210063 2307 36-37 0.16092763812680033 2307 38-39 0.3072190453916761 2307 40-41 0.42558194006379324 2307 42-43 0.2804325664257732 2307 44-45 0.34338891220956214 2307 46-47 0.2836750770843963 2307 48-49 0.18249123236964948 2307 50-51 0.030616913621372532 2307 52-53 0.09547692160335686 2307 54-55 0.19881435569218553 2307 56-57 0.26748568315828436 2307 58-59 0.3981622661708215 2307 60-61 0.4267903673887119 2307 62-63 0.30783026954025416 2307 64-65 0.3154990303099865 2307 66-67 0.29782264202762576 2307 68-69 0.227166298221249 2307 70-71 0.1655017528026974 2307 72-73 -0.031068225023624052 2307 74-75 0.1995165925779716 2307 76-77 0.25554527845983444 2307 78-79 0.283829623224392 2307 80-81 0.3279930425912738 2307 82-83 0.23158203901298435 2307 84-85 0.30767161669266585 2307 86-87 0.29154300760156104 2307 88-89 0.3837047977900312 2307 90-91 0.49268201185533655 2307 92-93 0.4472014893686591 2307 94-95 0.3350704135696745 2307 96-97 0.2408230172634731 2307 98-99 0.21468020535940724 2307 100-101 0.2464517062307614 2201 1 0.3238180917314182 2201 2 0.24766785116189283 2201 3 0.27849438146602523 2201 4 0.3338635726209347 2201 5 0.27527932751799256 2201 6 0.4014931536804056 2201 7 0.18349650832207232 2201 8 0.30293031983180185 2201 9 0.27727767473843556 2201 10-11 0.32733408174291156 2201 12-13 0.3157516258800612 2201 14-15 0.4389802411175623 2201 16-17 0.4472615154471953 2201 18-19 0.47483400643250917 2201 20-21 0.4012729719884689 2201 22-23 0.4355822472712916 2201 24-25 0.3742602849549499 2201 26-27 0.4686856713029641 2201 28-29 0.4111719840765957 2201 30-31 0.3543273073817872 2201 32-33 0.30328349414217826 2201 34-35 0.43740348535963136 2201 36-37 0.38159008956607465 2201 38-39 0.36539876889397505 2201 40-41 0.40954803979684584 2201 42-43 0.3603054585106449 2201 44-45 0.27114032472068317 2201 46-47 0.20640294171482765 2201 48-49 0.3478709645532021 2201 50-51 0.2520928701862104 2201 52-53 0.2714969680480195 2201 54-55 0.4245441229624447 2201 56-57 0.44362780962957515 2201 58-59 0.3303787330482564 2201 60-61 0.42449797577769033 2201 62-63 0.3545876431738648 2201 64-65 0.2921361686818216 2201 66-67 0.25690141710376935 2201 68-69 0.3270366221040888 2201 70-71 0.43293261029279506 2201 72-73 0.4050463023186808 2201 74-75 0.47978393879331094 2201 76-77 0.4245156103004746 2201 78-79 0.3859183672650772 2201 80-81 0.34587423973514575 2201 82-83 0.4123926865616241 2201 84-85 0.23414290708758756 2201 86-87 0.3277895622319278 2201 88-89 0.38609195967357834 2201 90-91 0.2884772140198564 2201 92-93 0.2273451278236891 2201 94-95 0.36464307921870187 2201 96-97 0.3313877685095932 2201 98-99 0.23119030815983876 2201 100-101 0.18329979888859427 1101 1 0.1663884107786444 1101 2 0.09128631647745777 1101 3 0.02318341960297232 1101 4 0.040734360265084035 1101 5 -0.010066690588494964 1101 6 0.13060636055945452 1101 7 0.18110985094078558 1101 8 0.15295805793687833 1101 9 0.1926917987454715 1101 10-11 0.1458371683448192 1101 12-13 0.04861714918597215 1101 14-15 0.251843639802658 1101 16-17 0.15735936603913103 1101 18-19 0.11575538365892868 1101 20-21 0.23259460416939248 1101 22-23 0.26148845706579493 1101 24-25 0.014460471449446288 1101 26-27 0.1410455348550883 1101 28-29 0.057979741303263665 1101 30-31 0.118401149758526 1101 32-33 -0.011750558808095946 1101 34-35 0.017258539006299145 1101 36-37 -0.1387094819687249 1101 38-39 -0.0012871767393320965 1101 40-41 -0.1048710534622046 1101 42-43 -0.1407273432740368 1101 44-45 -0.1360090243191081 1101 46-47 -0.08033824405966072 1101 48-49 -0.11193357033189244 1101 50-51 -0.05687539033952049 1101 52-53 -0.08766781990301808 1101 54-55 0.08954925746274256 1101 56-57 -0.029446162585102797 1101 58-59 -0.07389411547809033 1101 60-61 0.06281669250672195 1101 62-63 0.10074385001750841 1101 64-65 0.045617595954873025 1101 66-67 -0.18185048620238575 1101 68-69 -0.1439670251616434 1101 70-71 0.011646270424293448 1101 72-73 -0.02119630577140441 1101 74-75 0.06019729557616671 1101 76-77 -0.10172817813450763 1101 78-79 -0.17169426714670522 1101 80-81 -0.11755631467285355 1101 82-83 -0.020836382972497347 1101 84-85 0.01658409640310765 1101 86-87 -0.19249820585390864 1101 88-89 -0.07594696810112112 1101 90-91 -0.21414787303057636 1101 92-93 -0.15685595573292233 1101 94-95 -0.0051318290094855 1101 96-97 0.05513158645320004 1101 98-99 -0.02144127078752689 1101 100-101 -0.3553482340666356 2304 1 0.17077918087143473 2304 2 0.1727154878255348 2304 3 0.19249060576498067 2304 4 0.25267631135475455 2304 5 0.19200192505938674 2304 6 0.3062750232689808 2304 7 0.22121446991327076 2304 8 0.319120326261654 2304 9 0.27100255355487235 2304 10-11 0.30420747244576063 2304 12-13 0.29306556397148853 2304 14-15 0.24668637061294874 2304 16-17 0.2965903449505092 2304 18-19 0.37229406943914967 2304 20-21 0.31136621947304377 2304 22-23 0.37461216221525717 2304 24-25 0.20919623602938486 2304 26-27 0.2005683250981889 2304 28-29 0.21366788101111922 2304 30-31 0.20536224876446596 2304 32-33 0.2458802134513931 2304 34-35 0.061047712148315725 2304 36-37 0.1114653974794848 2304 38-39 0.13946005503985504 2304 40-41 0.19529124514977525 2304 42-43 0.26278608822476457 2304 44-45 0.19133195769670408 2304 46-47 0.2548073703831406 2304 48-49 0.37660592854312114 2304 50-51 0.17910484027419926 2304 52-53 0.21902632074723982 2304 54-55 0.3582817440359065 2304 56-57 0.37268235764645397 2304 58-59 0.29481976194146853 2304 60-61 0.42179978817291186 2304 62-63 0.4996641113960294 2304 64-65 0.4641259572601726 2304 66-67 0.4144092195341855 2304 68-69 0.4436566063083802 2304 70-71 0.3248004666282611 2304 72-73 0.5462018841025369 2304 74-75 0.47107663196035077 2304 76-77 0.4365367847607615 2304 78-79 0.31377341087940636 2304 80-81 0.29656273265496935 2304 82-83 0.3458418452497014 2304 84-85 0.4068828446681465 2304 86-87 0.4211928018186484 2304 88-89 0.48073668851893103 2304 90-91 0.4407212018178299 2304 92-93 0.4927970228674212 2304 94-95 0.5290848421128942 2304 96-97 0.378078158087952 2304 98-99 0.2707241881771054 2304 100-101 0.42717177150155194 2202 1 0.22799578908293938 2202 2 0.2290885475192539 2202 3 0.18601423669876027 2202 4 0.08218690852343968 2202 5 0.14389940280591418 2202 6 0.2077158562261232 2202 7 0.2856381367304195 2202 8 0.21478509458242456 2202 9 0.33044306240417853 2202 10-11 0.3094959284191887 2202 12-13 0.24308929329514228 2202 14-15 0.33609383203832977 2202 16-17 0.36759455835236565 2202 18-19 0.34202037194751966 2202 20-21 0.2525131560752385 2202 22-23 0.24914119301708126 2202 24-25 0.09193214023236607 2202 26-27 0.22704316043983397 2202 28-29 0.18844751662130932 2202 30-31 0.2136154232849634 2202 32-33 0.02267264553344006 2202 34-35 0.12424946348815524 2202 36-37 0.03530729073225558 2202 38-39 0.04821212412112885 2202 40-41 0.16898569827567655 2202 42-43 0.1213354452179729 2202 44-45 0.0775498507642709 2202 46-47 0.11981066933789464 2202 48-49 0.15239131127974304 2202 50-51 0.14623683319012315 2202 52-53 0.08993301241650897 2202 54-55 0.24524117846772953 2202 56-57 0.20149771457020904 2202 58-59 0.14105085082804436 2202 60-61 0.20605899920183646 2202 62-63 0.26414985065127183 2202 64-65 0.2233239639969895 2202 66-67 0.2533073686469436 2202 68-69 0.2061499505224802 2202 70-71 0.14804883740761454 2202 72-73 0.2529320411790863 2202 74-75 0.2517612518860446 2202 76-77 0.2925005136214054 2202 78-79 0.18458532994505816 2202 80-81 0.1377549575395065 2202 82-83 0.21135300960709458 2202 84-85 0.18846093150590093 2202 86-87 0.3287157208727223 2202 88-89 0.25330052618720345 2202 90-91 0.15095794522860473 2202 92-93 0.10295027957240421 2202 94-95 0.23712445272048654 2202 96-97 0.16640390961300255 2202 98-99 0.2446892134690941 2202 100-101 0.3642565083375615 1102 1 0.06731042238975249 1102 2 0.018495298488396372 1102 3 0.09982426835451008 1102 4 0.13166946964837223 1102 5 0.1361752028047789 1102 6 0.15684165537857808 1102 7 0.22919596630850947 1102 8 0.20722735528810432 1102 9 0.1733373062493584 1102 10-11 0.2580715608288031 1102 12-13 0.19418229122425856 1102 14-15 0.3060095859924701 1102 16-17 0.2827849442590633 1102 18-19 0.24828536046454275 1102 20-21 0.22143367249842072 1102 22-23 0.1635214936620457 1102 24-25 0.16186009660273726 1102 26-27 0.12687648813042784 1102 28-29 0.0950718768761405 1102 30-31 0.030038888037616118 1102 32-33 0.15651904462157518 1102 34-35 0.0842350604049642 1102 36-37 0.03291406540088104 1102 38-39 0.11601241641483995 1102 40-41 0.006796895156369942 1102 42-43 -0.030500348348176942 1102 44-45 0.0023096112865630403 1102 46-47 0.04889840823722125 1102 48-49 0.17087197644544716 1102 50-51 0.0352343447436283 1102 52-53 0.07223033945255253 1102 54-55 0.21701303983699916 1102 56-57 0.3313388292362376 1102 58-59 0.32585327043316425 1102 60-61 0.39701658557802233 1102 62-63 0.295379924740395 1102 64-65 0.22914938339332735 1102 66-67 0.1261469061683158 1102 68-69 0.16090896338227623 1102 70-71 0.222920888372812 1102 72-73 0.24334291698811228 1102 74-75 0.2710445962227084 1102 76-77 0.35768363450573304 1102 78-79 0.3208316551133521 1102 80-81 0.3187545258702187 1102 82-83 0.44419415530817474 1102 84-85 0.4402493260911271 1102 86-87 0.22922123946835882 1102 88-89 0.27911992196305846 1102 90-91 0.4171508336886447 1102 92-93 0.35153536356452264 1102 94-95 0.44400548692110675 1102 96-97 0.24308163604188593 1102 98-99 0.23418490806165693 1102 100-101 0.09546655071450871 2305 1 0.12796451220437177 2305 2 0.10370931536662198 2305 3 0.07434796099307306 2305 4 0.10496820495005466 2305 5 0.112597111774555 2305 6 0.21341411254014275 2305 7 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234.0 6 416.0 7 1442.0 8 5687.0 9 8678.0 10 9731.0 11 9272.0 12 7121.0 13 5801.0 14 4768.0 15 4481.0 16 4462.0 17 4760.0 18 5127.0 19 5773.0 20 6596.0 21 7339.0 22 8251.0 23 9228.0 24 10404.0 25 12316.0 26 14510.0 27 17258.0 28 21102.0 29 25960.0 30 32440.0 31 41994.0 32 58055.0 33 84335.0 34 128299.0 35 220198.0 36 444979.0 37 848393.0 38 324236.0 39 686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.88199325632731 24.804327259678242 26.22085820805008 13.092821275944361 2 31.638837280697718 22.201802015828495 35.1312781329704 11.028082570503388 3 39.94649683020061 18.36225777021066 26.873426285125568 14.817819114463166 4 30.71016736753328 17.86225159027799 36.86576519205222 14.561815850136501 5 30.620417124039516 16.551732789713032 38.18623177042497 14.641618315822488 6 23.478630302072343 17.49020572063692 44.4797647616694 14.551399215621338 7 33.84126179948216 17.216856319489555 32.669402148423295 16.272479732604985 8 30.03206371283752 28.075934829417037 26.598421221563935 15.293580236181509 9 30.80392909966979 27.63037408195139 24.839188685971685 16.72650813240713 10-11 24.76558770470141 28.81330897338268 30.551556498476458 15.869546823439453 12-13 25.871642051851012 26.343440533785262 29.84564338873879 17.939274025624936 14-15 23.800698217843465 27.30163002478828 31.152611128270046 17.745060629098205 16-17 22.02410218594661 28.839981219075884 32.480300193889846 16.655616401087656 18-19 21.1149871362587 29.361991029650962 32.30057021730445 17.222451616785886 20-21 21.153081095537782 29.501500221481162 31.759103711690027 17.586314971291024 22-23 21.239365367506238 29.041933433541985 31.942029421560402 17.77667177739137 24-25 20.802465692407516 29.67733100582618 31.50372089375268 18.016482408013626 26-27 20.565704603380276 29.91851679374032 31.1232386904727 18.392539912406704 28-29 20.273092382942895 29.75370890905121 30.920455693210492 19.052743014795404 30-31 20.198110299689066 30.046163797045022 30.60744910965037 19.14827679361554 32-33 19.554411665280643 30.10428765584383 31.905454342988605 18.43584633588692 34-35 19.92152997497593 30.109402062261424 31.62062311673091 18.34844484603173 36-37 20.07746745676077 30.604648047405647 31.535387829173896 17.78249666665969 38-39 20.768326580051262 30.080002781030107 30.74650763977032 18.40516299914831 40-41 20.27719731970416 29.68137196905083 30.58614666744007 19.45528404380494 42-43 20.379365744753102 30.133335679649125 31.23748675221921 18.249811823378565 44-45 19.96051180122975 30.453697584640604 31.422098649720535 18.16369196440911 46-47 20.30708477861415 30.068948299143333 31.120295188112884 18.50367173412963 48-49 20.11515365269682 30.448383383626577 30.55505057528655 18.88141238839005 50-51 19.9526640258117 30.12388669446051 30.384425169968992 19.5390241097588 52-53 20.423910946697166 29.87728926719363 30.304610067151906 19.394189718957296 54-55 20.86058607734918 29.916744023102858 30.07130333432241 19.15136656522556 56-57 20.94519679801893 30.255231953462285 29.81567372330618 18.983897525212605 58-59 20.741581819792422 30.14212475322873 30.433192863669174 18.683100563309672 60-61 20.177572012885474 30.650464426035494 29.976566724749144 19.195396836329888 62-63 20.55229559949901 30.50782871014196 29.91630476338833 19.023570926970702 64-65 20.1311034139525 30.357006388297343 30.2288677506665 19.283022447083663 66-67 20.48328275624075 29.52331334540036 30.206606655403732 19.786797242955153 68-69 20.403996710305922 29.6593391131896 30.873900364479347 19.06276381202514 70-71 19.70208702791908 30.794898650678494 30.51388857195673 18.989125749445694 72-73 19.58304560450475 31.37582140082895 30.009019631566158 19.03211336310014 74-75 20.08786689883479 30.56218570742546 29.880209286250487 19.469738107489263 76-77 20.108275652462954 30.31458715511835 30.147572882190367 19.42956431022833 78-79 19.928766919112732 30.488441057284142 30.443119668980117 19.13967235462301 80-81 19.231649323419717 30.77160613365797 30.320071096670326 19.676673446251986 82-83 18.65639714405079 30.67506824185731 30.283804456059784 20.38473015803212 84-85 18.725444413964095 30.386397760182938 30.742745618682456 20.145412207170512 86-87 18.833188397633762 30.256208486287083 31.43869952787885 19.471903588200302 88-89 19.04454955897342 30.775465020798475 30.831233381269413 19.34875203895869 90-91 19.296295637079016 30.462676300390424 30.743521474332464 19.497506588198096 92-93 18.719504931237772 31.541707152288957 30.54930431725905 19.18948359921422 94-95 18.43079384990533 31.783493120458754 30.556534831471964 19.22917819816395 96-97 18.98459389909864 31.757265056019328 30.33066957365193 18.9274714712301 98-99 19.356906747332832 30.876666691791094 29.945555362934932 19.820871197941138 100-101 19.190029743771415 30.881555469851506 29.574952587096735 20.353462199280337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 550.0 1 477.5 2 300.0 3 222.5 4 268.5 5 292.5 6 380.5 7 568.5 8 713.0 9 849.0 10 1011.0 11 1153.0 12 1176.5 13 1070.0 14 973.5 15 912.0 16 850.5 17 850.0 18 955.5 19 1141.0 20 1522.0 21 2089.5 22 2950.5 23 4201.5 24 5916.5 25 8331.0 26 11895.5 27 16824.5 28 22315.5 29 27764.5 30 33972.0 31 40771.0 32 49061.5 33 60822.0 34 75643.5 35 94089.5 36 120340.0 37 148262.5 38 172895.5 39 184333.5 40 176235.0 41 158229.0 42 140996.5 43 131529.0 44 118676.0 45 103461.5 46 96643.5 47 86570.0 48 67452.5 49 50389.0 50 38349.0 51 30443.0 52 25046.0 53 19485.5 54 14354.5 55 10471.5 56 7576.0 57 5462.5 58 4033.5 59 2981.5 60 2210.5 61 1620.5 62 1188.0 63 886.0 64 653.5 65 469.5 66 348.0 67 288.5 68 225.0 69 172.0 70 156.5 71 118.5 72 84.0 73 76.5 74 56.5 75 41.0 76 38.0 77 28.5 78 23.5 79 23.5 80 14.0 81 9.0 82 9.5 83 8.0 84 6.0 85 3.5 86 3.0 87 4.0 88 4.0 89 3.5 90 1.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19289506650308919 2 0.19723635343585727 3 0.1656785368861201 4 0.16864230008060602 5 0.176490011074456 6 0.1734845047363858 7 0.1392968701408372 8 0.11967759265621224 9 0.12072117124581995 10-11 0.09728239612323077 12-13 0.14526613967339327 14-15 0.12272484213786675 16-17 0.114772773285056 18-19 0.11817483948717714 20-21 0.109492265621641 22-23 0.10836520074486465 24-25 0.47436908369208086 26-27 0.32346761963480597 28-29 0.39756169949695336 30-31 0.25782652634848097 32-33 0.2869214974267439 34-35 0.3048293060244122 36-37 0.28627447870118716 38-39 0.3338825339590909 40-41 0.32899858615972677 42-43 0.37078347288761954 44-45 0.32563826310119 46-47 0.3497866716647124 48-49 0.3452575405858149 50-51 0.35997199869928365 52-53 0.3507885071107358 54-55 0.32753757613427603 56-57 0.34254423625283487 58-59 0.3346756536871927 60-61 0.3060816003319415 62-63 0.31449284376417963 64-65 0.31576600964350104 66-67 0.3268070711215506 68-69 0.3147015594821012 70-71 0.30282563513236543 72-73 0.28905039774954366 74-75 0.296543292022927 76-77 0.2874015435779635 78-79 0.29662677831009565 80-81 0.30495453545516515 82-83 0.33208757878496564 84-85 0.330104779464711 86-87 0.3240311520731941 88-89 0.33596969113830627 90-91 0.32603482296524094 92-93 0.30929582238793324 94-95 0.30126026724795385 96-97 0.2869423689985361 98-99 0.31261440230288573 100-101 0.31474330262568545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2395603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.254936636955485 #Duplication Level Percentage of deduplicated Percentage of total 1 91.91470169534843 56.30229228353232 2 4.141875082784265 5.074205915082699 3 1.1342545910493111 2.0843607931470425 4 0.6391891691350304 1.5661396821757805 5 0.3832682518820446 1.1738536241995667 6 0.2629503032507644 0.9664202498576283 7 0.17138950026240396 0.7348917085169114 8 0.14785560271499582 0.7245508460580746 9 0.14016236001125576 0.7727072833238053 >10 0.8538764346979758 10.241891228647734 >50 0.10285136527054767 4.407983293955267 >100 0.10109787084783126 12.11044570256275 >500 0.005415544574850071 2.244782461235343 >1k 9.740244379335356E-4 0.9588572302831663 >5k 1.3820373238071287E-4 0.6366176974218418 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7588 0.3167469735177323 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7452 0.31106990599026635 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3736 0.15595238443097625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.174314358430842E-4 0.0 0.0 2 5.00917723011701E-4 0.0 0.0 3 5.844040101803178E-4 0.0 4.174314358430842E-5 4 5.844040101803178E-4 0.0 4.174314358430842E-5 5 6.678902973489347E-4 0.0 4.174314358430842E-5 6 6.678902973489347E-4 0.0 4.174314358430842E-5 7 7.9311972810186E-4 0.0 4.174314358430842E-5 8 0.0010435785896077104 0.0 4.174314358430842E-5 9 0.0010853217331920188 0.0 4.174314358430842E-5 10-11 0.0018366983177095703 0.0 4.174314358430842E-5 12-13 0.002671561189395739 0.0 4.174314358430842E-5 14-15 0.00356903877645837 0.0 4.174314358430842E-5 16-17 0.004675232081442543 0.0 4.174314358430842E-5 18-19 0.005739682242842407 0.0 4.174314358430842E-5 20-21 0.0068458755478265805 0.0 4.174314358430842E-5 22-23 0.008640830721951844 0.0 4.174314358430842E-5 24-25 0.01070711632937511 0.0 4.174314358430842E-5 26-27 0.013002989226512073 0.0 4.174314358430842E-5 28-29 0.015841522990245042 0.0 4.174314358430842E-5 30-31 0.02469106943011843 0.0 4.174314358430842E-5 32-33 0.027842676770733717 0.0 4.174314358430842E-5 34-35 0.030806439965219614 0.0 4.174314358430842E-5 36-37 0.03377020315970551 0.0 4.174314358430842E-5 38-39 0.036942682072112945 0.0 4.174314358430842E-5 40-41 0.040908280712622244 0.0 8.348628716861684E-5 42-43 0.0453956686479354 0.0 8.348628716861684E-5 44-45 0.04965346929353487 0.0 8.348628716861684E-5 46-47 0.05491310538515773 0.0 1.0435785896077104E-4 48-49 0.06013099833319628 0.0 1.6697257433723368E-4 50-51 0.06622549729650531 0.0 1.6697257433723368E-4 52-53 0.07302962970074758 0.0 1.6697257433723368E-4 54-55 0.07991724839215847 0.0 2.087157179215421E-4 56-57 0.08759798681167122 0.0 2.087157179215421E-4 58-59 0.09609271653107798 0.0 2.504588615058505E-4 60-61 0.105839740558014 0.0 2.504588615058505E-4 62-63 0.11489800271580893 0.0 3.5481672046662157E-4 64-65 0.12508332975038017 0.0 4.174314358430842E-4 66-67 0.13658356580785713 0.0 4.174314358430842E-4 68-69 0.14912738045494184 0.0 4.174314358430842E-4 70-71 0.16359137970690468 0.0 4.174314358430842E-4 72-73 0.18112350001231423 0.0 4.591745794273926E-4 74-75 0.20111846578919795 0.0 4.800461512195468E-4 76-77 0.2215099914301326 0.0 5.00917723011701E-4 78-79 0.2453035832731884 0.0 5.00917723011701E-4 80-81 0.271100846008291 0.0 5.00917723011701E-4 82-83 0.30126026724795385 0.0 5.00917723011701E-4 84-85 0.33738895802017277 0.0 5.00917723011701E-4 86-87 0.37775457786619904 0.0 5.00917723011701E-4 88-89 0.4225658425039541 0.0 5.00917723011701E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 655 0.0 54.291264 4 GAGCGGC 440 0.0 52.793533 9 CGACGGG 100 0.0 52.16344 1 GATCCGG 95 7.2759576E-12 49.917168 1 TTGTGTT 31955 0.0 47.74037 1 TCGGGAG 840 0.0 47.41437 3 GGTCCGG 95 3.9108272E-10 44.92545 1 ACGAGCG 45 9.706037E-4 42.152275 1 AACCGGA 125 3.6379788E-12 41.729008 2 GTTCGGA 80 2.2943823E-7 41.49191 2 AGAGCGG 515 0.0 41.423103 8 ATCCGGA 115 5.638867E-11 41.2342 2 GAACCGG 130 5.456968E-12 40.125725 1 ACCGGAA 120 8.913048E-11 39.51197 3 GATCGGG 1130 0.0 38.188843 1 TCGGAGA 225 0.0 37.931496 4 TGTGTTT 54520 0.0 36.192238 1 GAGAGCG 735 0.0 36.11919 7 GGCGCCG 200 0.0 35.62894 56-57 TCAGCCG 80 1.0907264E-5 35.56449 2 >>END_MODULE