FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395603
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG75880.3167469735177323No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC74520.31106990599026635No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA37360.15595238443097625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA6550.054.2912644
GAGCGGC4400.052.7935339
CGACGGG1000.052.163441
GATCCGG957.2759576E-1249.9171681
TTGTGTT319550.047.740371
TCGGGAG8400.047.414373
GGTCCGG953.9108272E-1044.925451
ACGAGCG459.706037E-442.1522751
AACCGGA1253.6379788E-1241.7290082
GTTCGGA802.2943823E-741.491912
AGAGCGG5150.041.4231038
ATCCGGA1155.638867E-1141.23422
GAACCGG1305.456968E-1240.1257251
ACCGGAA1208.913048E-1139.511973
GATCGGG11300.038.1888431
TCGGAGA2250.037.9314964
TGTGTTT545200.036.1922381
GAGAGCG7350.036.119197
GGCGCCG2000.035.6289456-57
TCAGCCG801.0907264E-535.564492
Produced by FastQC (version 0.11.2)