##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C68Y0ACXX_l03n02_50330665-1.342000000356cf.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2794520
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.463732590927957	33.0	33.0	33.0	22.0	33.0
2	29.593646493852255	33.0	33.0	33.0	15.0	33.0
3	29.39088680703663	33.0	33.0	33.0	15.0	33.0
4	32.457705795628584	37.0	37.0	37.0	15.0	37.0
5	32.629244020440005	37.0	37.0	37.0	15.0	37.0
6	33.647157293560255	37.0	37.0	37.0	22.0	37.0
7	32.5240982351173	37.0	37.0	37.0	15.0	37.0
8	33.18510799708	37.0	37.0	37.0	22.0	37.0
9	32.13610494825587	37.0	37.0	37.0	6.0	37.0
10-11	32.23544186479252	37.0	33.0	37.0	15.0	37.0
12-13	32.210214455434205	37.0	33.0	37.0	10.5	37.0
14-15	33.56586730458183	37.0	33.0	40.0	15.0	40.0
16-17	33.40139236792007	37.0	33.0	40.0	10.5	40.0
18-19	33.57821414768905	37.0	33.0	40.0	10.5	40.0
20-21	33.5883679486996	37.0	33.0	40.0	10.5	40.0
22-23	34.971719472395975	37.0	33.0	40.0	22.0	40.0
24-25	33.85003685785036	37.0	33.0	40.0	15.0	40.0
26-27	33.68511139659047	37.0	33.0	40.0	10.5	40.0
28-29	33.16355993158038	37.0	33.0	40.0	10.5	40.0
30-31	33.12120668307974	37.0	33.0	40.0	10.5	40.0
32-33	32.86390006154903	37.0	33.0	40.0	6.0	40.0
34-35	33.38944112047865	37.0	33.0	40.0	10.5	40.0
36-37	32.89574524426378	37.0	33.0	40.0	6.0	40.0
38-39	32.81789913831356	37.0	33.0	40.0	6.0	40.0
40-41	32.546687266507305	37.0	33.0	40.0	6.0	40.0
42-43	32.45504827304868	37.0	33.0	40.0	6.0	40.0
44-45	32.26530549074617	37.0	33.0	40.0	6.0	40.0
46-47	32.23077648397578	37.0	33.0	40.0	6.0	40.0
48-49	32.131570359131445	37.0	33.0	40.0	6.0	40.0
50-51	31.271708558178148	37.0	30.0	38.5	10.5	38.5
52-53	31.771625538554026	37.0	33.0	38.5	10.5	40.0
54-55	32.257209109256685	37.0	33.0	40.0	10.5	40.0
56-57	32.70714076120407	37.0	33.0	40.0	10.5	40.0
58-59	31.851754147402772	37.0	33.0	40.0	6.0	40.0
60-61	31.77235965389405	37.0	33.0	40.0	6.0	40.0
62-63	31.22809319668494	37.0	33.0	37.0	6.0	40.0
64-65	31.067032084221978	37.0	33.0	37.0	6.0	40.0
66-67	30.990622718749552	37.0	33.0	37.0	6.0	40.0
68-69	31.36462880924094	37.0	33.0	37.0	10.5	38.5
70-71	30.84308217511415	37.0	27.0	37.0	10.5	37.0
72-73	31.51428724789946	37.0	27.0	37.0	15.0	37.0
74-75	31.86218474013426	37.0	27.0	37.0	22.0	37.0
76-77	31.502389140174344	33.0	27.0	37.0	22.0	37.0
78-79	30.936989536664615	33.0	27.0	37.0	15.0	37.0
80-81	30.297028112162373	33.0	27.0	37.0	15.0	37.0
82-83	29.686249516911673	33.0	27.0	37.0	6.0	37.0
84-85	29.416727201809255	33.0	27.0	37.0	6.0	37.0
86-87	29.195559702560725	33.0	27.0	37.0	6.0	37.0
88-89	29.09530187652978	33.0	27.0	37.0	6.0	37.0
90-91	28.979123964043914	33.0	27.0	37.0	6.0	37.0
92-93	28.792613221590827	33.0	27.0	37.0	6.0	37.0
94-95	28.596576156191404	33.0	27.0	37.0	6.0	37.0
96-97	28.420713932983123	33.0	22.0	37.0	6.0	37.0
98-99	28.30167488513233	33.0	22.0	37.0	6.0	37.0
100-101	27.102719250533184	33.0	18.5	35.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	-0.29183343998915134
2306	2	-0.3329425559848538
2306	3	-0.5396891175767955
2306	4	-0.285206822073377
2306	5	-0.40014858256337504
2306	6	-0.5171169409396867
2306	7	0.4440810902506698
2306	8	-0.5575843017766076
2306	9	-0.23949513080288654
2306	10-11	-0.2229260293342179
2306	12-13	-0.21051064108210227
2306	14-15	0.1732594348058356
2306	16-17	0.13512450326918923
2306	18-19	0.05239924410393115
2306	20-21	-0.03873683582288123
2306	22-23	-0.08786533374917127
2306	24-25	0.252429453479607
2306	26-27	-0.26232528905404706
2306	28-29	-0.07979092673828347
2306	30-31	0.006715745361859149
2306	32-33	-0.029827346832526302
2306	34-35	-0.38358761395928553
2306	36-37	0.10668376598752616
2306	38-39	-0.12496261515514107
2306	40-41	-0.0424488807844412
2306	42-43	-0.03597082696884257
2306	44-45	0.058055624952380924
2306	46-47	-0.04454992895595211
2306	48-49	-0.06678565488486043
2306	50-51	-0.013470929450626556
2306	52-53	-0.33524421753018885
2306	54-55	-0.0025498294637671393
2306	56-57	0.13795679435172303
2306	58-59	0.31713048958659584
2306	60-61	0.12235466338142587
2306	62-63	0.1653653935643149
2306	64-65	0.17908657103874503
2306	66-67	0.03649220343533344
2306	68-69	-0.11526044070893349
2306	70-71	0.08921754283176142
2306	72-73	-0.36995297527517934
2306	74-75	-0.2483460158486821
2306	76-77	0.09490688660210367
2306	78-79	0.1288202876283897
2306	80-81	0.48934155339181373
2306	82-83	0.33769832411243783
2306	84-85	0.12352750960342007
2306	86-87	0.26822302299612844
2306	88-89	0.15185054948784327
2306	90-91	0.030671795641147526
2306	92-93	0.028255736553969513
2306	94-95	0.22140179949636263
2306	96-97	0.08767344822458867
2306	98-99	0.18228963835979428
2306	100-101	-0.0753521041858285
2307	1	0.002465215911591656
2307	2	-0.061497921756505036
2307	3	-0.06085549325550588
2307	4	0.18519761962740233
2307	5	-0.05133923459101197
2307	6	-0.2733986408479936
2307	7	0.627706274195873
2307	8	-0.18673182699030377
2307	9	0.2306139044678801
2307	10-11	0.12229583478134742
2307	12-13	0.19906279960748208
2307	14-15	0.5964426116200485
2307	16-17	0.5208499764349668
2307	18-19	0.5442035041003024
2307	20-21	0.38714224027036437
2307	22-23	0.062082359955603295
2307	24-25	0.4315161300184016
2307	26-27	0.12035469951683808
2307	28-29	0.35142019333601837
2307	30-31	0.37029078595919884
2307	32-33	0.46996719420814514
2307	34-35	0.10735641157190656
2307	36-37	0.4342990702287963
2307	38-39	0.22953572623453766
2307	40-41	0.38023595548170874
2307	42-43	0.33485082371348085
2307	44-45	0.40003243681004363
2307	46-47	0.30540664377399906
2307	48-49	0.26278303489505106
2307	50-51	0.039722216278583744
2307	52-53	0.07243359336509059
2307	54-55	0.2759621511185202
2307	56-57	0.32584601441563166
2307	58-59	0.6259677075986474
2307	60-61	0.3526996156135347
2307	62-63	0.48262001160421875
2307	64-65	0.3577885248871695
2307	66-67	0.3636308386828766
2307	68-69	0.1587958262206577
2307	70-71	0.28987215001484756
2307	72-73	-0.3033069491859912
2307	74-75	-0.20309160232092083
2307	76-77	0.13506375078289778
2307	78-79	0.1498636824963171
2307	80-81	0.3815356699224992
2307	82-83	0.3647568377299599
2307	84-85	0.2882226960355929
2307	86-87	0.3952952338776079
2307	88-89	0.49006292819552044
2307	90-91	0.43938184581395134
2307	92-93	0.5283652515445851
2307	94-95	0.5981382064873131
2307	96-97	0.5923788055590613
2307	98-99	0.3338988880316691
2307	100-101	0.34804663095285093
2201	1	-0.1740080574713474
2201	2	-0.5566056401261967
2201	3	-0.38337504853171467
2201	4	-0.3785789744363086
2201	5	-0.5137104705734217
2201	6	-0.4014748245913182
2201	7	0.26732171928445325
2201	8	-0.40888245675036927
2201	9	-0.24910280006280772
2201	10-11	-0.22539305519995523
2201	12-13	-0.2977208066160273
2201	14-15	-0.012128137533615302
2201	16-17	-0.08623625327189188
2201	18-19	-0.08750848430133118
2201	20-21	-0.132158073136857
2201	22-23	-0.06080005400956878
2201	24-25	-0.014271599696840553
2201	26-27	-0.2815554341913469
2201	28-29	-0.2966914433066776
2201	30-31	-0.11371337069525111
2201	32-33	-0.1992586591369374
2201	34-35	-0.41342578777193495
2201	36-37	-0.07666901201786658
2201	38-39	-0.17417250970768094
2201	40-41	-0.009799680788013632
2201	42-43	-0.1181118557407288
2201	44-45	-0.06892754905093312
2201	46-47	-0.15910183203224193
2201	48-49	-0.16047199150052194
2201	50-51	-0.114482485102819
2201	52-53	-0.3227066634860378
2201	54-55	-0.0999698676445604
2201	56-57	0.07816321076897736
2201	58-59	0.1708041939264291
2201	60-61	-0.042672390134153915
2201	62-63	0.08619177319624782
2201	64-65	-0.02034842466530762
2201	66-67	-0.05649538253431885
2201	68-69	-0.2753025320685971
2201	70-71	-0.0948609622596912
2201	72-73	-0.41721387274280275
2201	74-75	-0.15297131918150697
2201	76-77	0.14685904784089487
2201	78-79	0.22846801183100496
2201	80-81	0.19767471769983302
2201	82-83	0.07208626722582068
2201	84-85	0.06409800654595088
2201	86-87	-0.012763170745824226
2201	88-89	0.1545960614601114
2201	90-91	0.07221044253536846
2201	92-93	-0.013474787876134542
2201	94-95	0.22910286321998896
2201	96-97	0.04694226905802523
2201	98-99	0.0045619551775963885
2201	100-101	-0.13017999449840545
1101	1	0.15284115576121593
1101	2	-0.13720392050412045
1101	3	0.05441255163310288
1101	4	0.17539729143662441
1101	5	0.12842704156449258
1101	6	0.13679238531610594
1101	7	0.7117851729932525
1101	8	0.011349467480222586
1101	9	0.2717621686883618
1101	10-11	0.23019892210610493
1101	12-13	0.12087285919294288
1101	14-15	0.5280267523535187
1101	16-17	0.4381159350105932
1101	18-19	0.37943820683806706
1101	20-21	0.3206259756971903
1101	22-23	0.2171234547916825
1101	24-25	0.29658185784423097
1101	26-27	0.3850552852088498
1101	28-29	0.38533622970003734
1101	30-31	0.5269247306898421
1101	32-33	0.48588831409193034
1101	34-35	0.20255667744230976
1101	36-37	0.3101867059848544
1101	38-39	0.21784542415256425
1101	40-41	0.3103929482916996
1101	42-43	0.3847877374049844
1101	44-45	0.4345232927513294
1101	46-47	0.36246313787149376
1101	48-49	0.3249987074993399
1101	50-51	0.2844798131628643
1101	52-53	0.16526779574641637
1101	54-55	0.5009833729267683
1101	56-57	0.4130104819350535
1101	58-59	0.41410961125462364
1101	60-61	0.36193764061043154
1101	62-63	0.6020074411201684
1101	64-65	0.5818308534276646
1101	66-67	0.48700950000523235
1101	68-69	0.31871606883673564
1101	70-71	0.41246554838854266
1101	72-73	0.052555874241090095
1101	74-75	0.1864773745338617
1101	76-77	0.3018521252671107
1101	78-79	0.35291622291115843
1101	80-81	0.518881961262057
1101	82-83	0.514807912046642
1101	84-85	0.5565409835552302
1101	86-87	0.4323939810436741
1101	88-89	0.5325592255877751
1101	90-91	0.3502162514464011
1101	92-93	0.49767498607769767
1101	94-95	0.5567592298268202
1101	96-97	0.4986891924386079
1101	98-99	0.4023032583791277
1101	100-101	0.31631124747328343
2304	1	0.13536941886501808
2304	2	-0.05436354828362511
2304	3	0.02985412528447995
2304	4	0.1626941649013105
2304	5	-0.022141540115015346
2304	6	0.13314414112075923
2304	7	0.45521269011577203
2304	8	0.19280098400786017
2304	9	0.18429792081755636
2304	10-11	0.32373953811828216
2304	12-13	0.3035856590527217
2304	14-15	0.4229231276121155
2304	16-17	0.43911779133284057
2304	18-19	0.37369861246704517
2304	20-21	0.35977223344182363
2304	22-23	0.35553747440655314
2304	24-25	0.21135137113725477
2304	26-27	0.0686778223950526
2304	28-29	0.368201100594014
2304	30-31	0.4597050844799
2304	32-33	0.3221769243996988
2304	34-35	0.14538995235061947
2304	36-37	0.27927572048754
2304	38-39	0.15566526794530233
2304	40-41	0.38066314409518753
2304	42-43	0.33370518152278095
2304	44-45	0.4321096090803209
2304	46-47	0.36355445750681525
2304	48-49	0.4120959732083662
2304	50-51	0.21820210443284083
2304	52-53	0.1631804290948864
2304	54-55	0.45228910574914494
2304	56-57	0.4711241239118351
2304	58-59	0.5233275525162355
2304	60-61	0.3104920860905942
2304	62-63	0.4995336062345892
2304	64-65	0.3704892954448127
2304	66-67	0.3864401216208968
2304	68-69	0.3028223054421382
2304	70-71	0.3399632512229509
2304	72-73	0.09212488348552128
2304	74-75	0.19734027889475314
2304	76-77	0.4407916381086636
2304	78-79	0.42313413496025376
2304	80-81	0.3383564481177572
2304	82-83	0.39854842793957346
2304	84-85	0.3308897769457637
2304	86-87	0.5016093728498312
2304	88-89	0.5152468296573467
2304	90-91	0.251811254513715
2304	92-93	0.37101501837080164
2304	94-95	0.5465325274677753
2304	96-97	0.5325149557482227
2304	98-99	0.39325949671071925
2304	100-101	0.47789659713375343
2202	1	-0.06102586818787259
2202	2	-0.3154016111510316
2202	3	-0.2753797649772842
2202	4	-0.30823272781663746
2202	5	-0.33072468532218124
2202	6	0.010482598607993054
2202	7	0.12637283117057052
2202	8	-0.11240338707909103
2202	9	-0.15792349548433648
2202	10-11	-0.2000458884056897
2202	12-13	-0.1383072510037593
2202	14-15	-0.13888252751208796
2202	16-17	-0.09951718579682733
2202	18-19	-0.12148648213019442
2202	20-21	-0.22518840297284726
2202	22-23	-0.14485681742844037
2202	24-25	-0.1297180945094567
2202	26-27	-0.10640180909319241
2202	28-29	-0.1179944681561409
2202	30-31	0.04200618381145205
2202	32-33	-0.25232595863970886
2202	34-35	-0.3315145732668654
2202	36-37	-0.29946480409162035
2202	38-39	-0.24567626141034538
2202	40-41	-0.12286838004072109
2202	42-43	-0.12037797633545466
2202	44-45	-0.09295334173135217
2202	46-47	-0.14394809847888013
2202	48-49	-0.06520307852328955
2202	50-51	-0.09792459561392164
2202	52-53	-0.20957365368812475
2202	54-55	0.09546928610662064
2202	56-57	0.1420246836516199
2202	58-59	0.011615937048933489
2202	60-61	-0.08145461446221347
2202	62-63	0.041140414574552864
2202	64-65	-0.04425839057986991
2202	66-67	0.02022430177970236
2202	68-69	-0.010097112833324928
2202	70-71	0.08338914356886207
2202	72-73	-0.023260182435951293
2202	74-75	-8.006222178842393E-4
2202	76-77	0.10013239528018403
2202	78-79	-0.011916417798008894
2202	80-81	0.07831383742319176
2202	82-83	0.09488795655115112
2202	84-85	-0.02397990292581298
2202	86-87	0.08399996682248556
2202	88-89	0.0633427023144364
2202	90-91	0.03248448849393881
2202	92-93	0.17822927041582304
2202	94-95	0.08306742616078111
2202	96-97	0.12366712194024032
2202	98-99	-6.539774337639415E-4
2202	100-101	0.16882497986719258
1102	1	-0.10035454408367883
1102	2	-0.4152927756991467
1102	3	-0.3024467131911237
1102	4	-0.2488162374095637
1102	5	-0.2834357619445882
1102	6	-0.004734174721512829
1102	7	-0.11966039531704098
1102	8	0.05897214292497921
1102	9	-0.18958687250839645
1102	10-11	-0.1231166048221084
1102	12-13	-0.06872951763408253
1102	14-15	-0.2107192658486312
1102	16-17	-0.12298857000265428
1102	18-19	-0.11379009368521764
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1213	5	2.0122033977278164
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1213	10-11	0.8616540341076941
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1213	92-93	-1.4626008475571446
1213	94-95	-1.6684265397611995
1213	96-97	-1.6054918801623579
1213	98-99	-1.5262462779798724
1213	100-101	-1.4325405703736216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	997.0
3	328.0
4	540.0
5	441.0
6	833.0
7	4944.0
8	25484.0
9	84385.0
10	113431.0
11	77336.0
12	44144.0
13	27912.0
14	19416.0
15	14982.0
16	12670.0
17	11395.0
18	10636.0
19	10462.0
20	10725.0
21	10836.0
22	11205.0
23	11750.0
24	12639.0
25	14242.0
26	16838.0
27	20975.0
28	27191.0
29	33744.0
30	39095.0
31	50388.0
32	70433.0
33	102812.0
34	159565.0
35	278430.0
36	525902.0
37	777512.0
38	159829.0
39	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.243054895852566	18.859387966897945	21.955652424849127	11.941904712400365
2	33.57003683554854	26.268603744499313	30.033107181587603	10.128252238364539
3	39.10081924529371	17.12817234005113	30.879264983687847	12.891743430967322
4	29.68343948428236	14.12435412544365	34.92437304875728	21.267833341516713
5	39.24197588212608	12.304925798325714	31.745678438861574	16.707419880686633
6	36.42316482976113	12.603844509300533	36.98278454354293	13.990206117395413
7	41.30854763387409	17.347111340736607	25.76955791757703	15.574783107812276
8	28.936369905338484	36.03961992101092	19.987605341166567	15.036404832484038
9	38.99085296610503	26.574902579467857	18.529066389253686	15.905178065173423
10-11	32.388512076645846	30.128265395205155	22.779307411635664	14.70391511651333
12-13	35.79640793657903	21.472154790164375	22.46252519371165	20.268912079544947
14-15	30.249858407479095	21.748467916979674	25.16395536244282	22.83771831309841
16-17	32.97880857400307	23.381852055300918	26.526312282802618	17.1130270878934
18-19	32.439783205209956	23.494418931214582	27.04538290143933	17.020414962136137
20-21	29.38492662611214	28.292780599735583	25.43558861908656	16.886704155065715
22-23	35.34485352537076	23.307290453985022	24.10598571898335	17.241870301660864
24-25	28.72266825848498	29.55057195635297	24.643508010947883	17.083251774214162
26-27	24.110408263695685	32.4128157922791	25.417296459508343	18.059479484516878
28-29	26.223137254972407	30.752172129947503	23.85862468565709	19.166065929423
30-31	29.89886316362023	25.560296136090997	25.46681403268447	19.0740266676043
32-33	24.53055240528862	28.21428315077736	27.52645027921733	19.728714164716692
34-35	32.85714695956011	23.80146341428394	24.26710828504703	19.074281341108918
36-37	25.963308086318378	25.243222640772682	28.028421554327977	20.765047718580963
38-39	19.65182887058832	30.204178505970386	29.5633256057322	20.580667017709093
40-41	23.266337756659496	31.7343641781313	24.98527014077114	20.014027924438064
42-43	28.676658375165008	25.52469041568261	27.38221427994145	18.41643692921093
44-45	23.25544573509121	29.72132154601395	27.46001889235915	19.563213826535687
46-47	26.62340040896094	30.675536105850064	24.312221640454812	18.38884184473418
48-49	20.807759321556716	28.174744535193387	29.73818268786036	21.27931345538954
50-51	18.505187897999384	25.37504267205255	30.991878976402287	25.12789045354578
52-53	28.143818161968355	25.4729679258076	24.494136203270624	21.889077708953423
54-55	31.032801464592808	25.110341729944334	25.374702311602242	18.48215449386061
56-57	32.50800375540459	24.80290679375241	23.465389537043503	19.223699913799496
58-59	28.566983875525537	24.25604690233934	23.462174971032535	23.714794251102596
60-61	21.57570893045792	23.94097023420003	24.656496688002395	29.826824147339654
62-63	26.926847078027738	23.64621026109286	25.555569715978027	23.871372944901378
64-65	22.826060421555084	28.300453491945422	28.383813870133	20.489672216366493
66-67	20.619862573504648	27.554863244911132	26.31676455318437	25.50850962839985
68-69	19.070298342001948	23.326134219437034	36.85906226754856	20.744505171012463
70-71	18.578138777816324	32.25038107875125	30.053917281367227	19.117562862065196
72-73	20.40364981029902	37.87001202701718	24.045420563364004	17.6809175993198
74-75	21.501897530822987	37.13783507400783	23.648837091603735	17.711430303565447
76-77	20.70346791889694	36.03615857978482	23.768017738333736	19.49235576298451
78-79	20.588992226525864	36.47579892843285	24.18917284305565	18.746036001985633
80-81	19.769796826764306	37.04723957211573	24.08329743700194	19.099666164118027
82-83	19.674841148488394	35.82898021036818	24.40921133206909	20.086967309074332
84-85	20.51887325946248	34.29422170806328	24.55827032911558	20.628634703358657
86-87	19.517981148211362	34.14062943173928	25.403636081636993	20.937753338412367
88-89	19.240567734855986	34.27860056977438	25.45219647521223	21.0286352201574
90-91	20.277959138904354	32.92991278569314	24.815067001691578	21.977061073710928
92-93	20.86387713185857	32.88264235140686	23.966775861812113	22.28670465492246
94-95	21.30706822557788	33.97139714761174	23.454094869979855	21.267439756830527
96-97	20.722652790695626	34.460944401785376	22.7490472592092	22.067355548309795
98-99	20.821756855647074	32.56935610702239	24.466249515596317	22.142637521734223
100-101	21.993674464689907	33.536142967149935	23.709538742108787	20.76064382605137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1002.0
1	786.0
2	460.5
3	463.0
4	486.5
5	387.5
6	444.5
7	617.0
8	791.5
9	890.0
10	980.5
11	1130.0
12	1256.5
13	1205.5
14	1052.5
15	972.5
16	934.0
17	850.5
18	751.5
19	701.0
20	690.0
21	718.0
22	782.5
23	829.0
24	923.5
25	1080.0
26	1313.0
27	1688.5
28	2095.0
29	2587.5
30	3251.5
31	4480.5
32	6891.0
33	10903.0
34	17173.0
35	27194.5
36	40959.5
37	59813.5
38	87054.5
39	111300.0
40	124163.5
41	126848.0
42	149090.0
43	187155.5
44	187900.0
45	172188.5
46	177368.5
47	179362.0
48	162893.0
49	140163.0
50	118210.5
51	107385.5
52	102586.0
53	101218.0
54	91583.5
55	63395.0
56	41910.0
57	34454.5
58	30902.0
59	25770.0
60	19591.5
61	14546.0
62	10292.0
63	7261.0
64	5285.0
65	3783.5
66	2754.0
67	2134.5
68	1594.0
69	1157.5
70	887.0
71	707.0
72	552.0
73	403.0
74	292.0
75	231.5
76	196.0
77	155.5
78	139.5
79	114.5
80	89.0
81	73.0
82	66.0
83	56.0
84	42.5
85	37.5
86	27.5
87	17.5
88	16.5
89	19.0
90	21.0
91	16.5
92	8.5
93	4.5
94	4.0
95	3.5
96	2.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17766915248414755
2	0.18232111418061062
3	0.15276326524769906
4	0.15426620671886407
5	0.16303336530065984
6	0.15784463879306643
7	0.12531669123856692
8	0.10717404062236091
9	0.10785394271645934
10-11	0.08793996822352318
12-13	0.13043384910467629
14-15	0.10946423714985043
16-17	0.10193163763365443
18-19	0.10520590298154961
20-21	0.09744070538053047
22-23	0.10169903954883128
24-25	0.45787827605456394
26-27	0.30599172666504443
28-29	0.39844051930206265
30-31	0.26321157121795513
32-33	0.2920000572549132
34-35	0.31153829638005814
36-37	0.2928588809527218
38-39	0.33928903711549746
40-41	0.3349770264660836
42-43	0.37734566222463967
44-45	0.3316133003163334
46-47	0.35433634398751845
48-49	0.3504000687058958
50-51	0.36512531669123854
52-53	0.3569843837224282
54-55	0.33311624178749843
56-57	0.3483066859424874
58-59	0.3399152627284829
60-61	0.3127191789645449
62-63	0.32128952378225956
64-65	0.3229177103760216
66-67	0.33358143795714473
68-69	0.32177261211227687
70-71	0.31055422755965245
72-73	0.29675937191360235
74-75	0.305276040250204
76-77	0.294791234272791
78-79	0.3044172165523954
80-81	0.3120392768704464
82-83	0.3381797231724948
84-85	0.3359789874468603
86-87	0.32971673131700613
88-89	0.3420265376522623
90-91	0.3311659963070581
92-93	0.31613658159540814
94-95	0.3093196684940526
96-97	0.2940218713768375
98-99	0.3177110917080572
100-101	0.3215221218670827
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2794520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.064108727391606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78137261330092	34.367542391641805
2	5.678268901238732	4.549895652851901
3	2.322190388929437	2.791094646833183
4	1.3917952592252856	2.230441463674801
5	0.7540423212998163	1.51050167728053
6	0.6032233335122931	1.450056313244167
7	0.4653937780912387	1.3051910848549264
8	0.35199534401473526	1.1281903787313574
9	0.2460513059755717	0.8872043647609804
>10	1.9638047895493016	15.09332670686548
>50	0.23093559914782688	6.464702027794518
>100	0.18770796808948087	15.335912601063646
>500	0.01590247704702425	4.2366130003039855
>1k	0.0065095639694859025	4.440974012826035
>5k	4.479758939853346E-4	1.4021145099588352
>10k+	3.583807149885197E-4	2.8062391673139166
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	25819	0.9239153772383093	Illumina Single End PCR Primer 1 (97% over 34bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA	21328	0.7632079927858809	No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC	19866	0.7108913158610423	No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC	11165	0.3995319410846943	No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT	9659	0.3456407540472067	No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT	9559	0.3420623219730043	No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC	7602	0.272032406280864	No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT	6522	0.2333853398794784	No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT	5719	0.20465053032363342	No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC	4384	0.15687846213303178	No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA	4169	0.1491848331734967	No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT	3911	0.1399524784220546	No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA	3377	0.12084365114581395	No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC	3049	0.10910639394243017	No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC	2942	0.10527747162303366	No Hit
TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA	2859	0.10230737300144568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.006691667978758427	0.0	0.0
2	0.007300001431372829	0.0	0.0
3	0.007872550563245208	0.0	0.0
4	0.008051472166955327	0.0	0.0
5	0.009554413638120322	0.0	3.5784320742023674E-5
6	0.010198531411476747	0.0	3.5784320742023674E-5
7	0.0114867669581896	0.0	3.5784320742023674E-5
8	0.013097061391580666	0.0	3.5784320742023674E-5
9	0.014814708787197801	0.0	3.5784320742023674E-5
10-11	0.023617651689735626	0.0	3.5784320742023674E-5
12-13	0.0311323590455606	0.0	3.5784320742023674E-5
14-15	0.037591428939495866	0.0	3.5784320742023674E-5
16-17	0.043567410503413825	0.0	3.5784320742023674E-5
18-19	0.049131872378798506	0.0	3.5784320742023674E-5
20-21	0.05417746160342385	0.0	3.5784320742023674E-5
22-23	0.05915148218656513	0.0	3.5784320742023674E-5
24-25	0.06503800294862802	0.0	3.5784320742023674E-5
26-27	0.06956471952249402	0.0	3.5784320742023674E-5
28-29	0.07643530910496257	0.0	3.5784320742023674E-5
30-31	0.1576299328686143	0.0	3.5784320742023674E-5
32-33	0.16875885661938367	0.0	3.5784320742023674E-5
34-35	0.1771323876730172	0.0	3.5784320742023674E-5
36-37	0.18790346821636633	0.0	3.5784320742023674E-5
38-39	0.20010592158939638	0.0	3.5784320742023674E-5
40-41	0.21456278716917393	1.7892160371011837E-5	3.5784320742023674E-5
42-43	0.23177504544608735	7.156864148404735E-5	3.5784320742023674E-5
44-45	0.2501681863074875	7.156864148404735E-5	3.5784320742023674E-5
46-47	0.2679708858766443	7.156864148404735E-5	3.5784320742023674E-5
48-49	0.28858265462405	7.156864148404735E-5	3.5784320742023674E-5
50-51	0.3125402573608348	7.156864148404735E-5	3.5784320742023674E-5
52-53	0.33676624250318477	7.156864148404735E-5	3.5784320742023674E-5
54-55	0.36537580693643273	7.156864148404735E-5	3.5784320742023674E-5
56-57	0.39600718549160496	7.156864148404735E-5	3.5784320742023674E-5
58-59	0.4281415055179423	7.156864148404735E-5	3.5784320742023674E-5
60-61	0.46455205187295134	7.156864148404735E-5	3.5784320742023674E-5
62-63	0.5028949515480298	7.156864148404735E-5	3.5784320742023674E-5
64-65	0.5459434894006843	7.156864148404735E-5	7.156864148404735E-5
66-67	0.5928030574123642	7.156864148404735E-5	7.156864148404735E-5
68-69	0.6447797832901536	7.156864148404735E-5	7.156864148404735E-5
70-71	0.6981163133561399	7.156864148404735E-5	7.156864148404735E-5
72-73	0.7560690207978472	7.156864148404735E-5	7.156864148404735E-5
74-75	0.8194251606716001	7.156864148404735E-5	7.156864148404735E-5
76-77	0.8897413509296767	7.156864148404735E-5	7.156864148404735E-5
78-79	0.9735124457867541	7.156864148404735E-5	7.156864148404735E-5
80-81	1.0660864835463693	7.156864148404735E-5	7.156864148404735E-5
82-83	1.1720438572635015	7.156864148404735E-5	7.156864148404735E-5
84-85	1.2961080972760974	7.156864148404735E-5	7.156864148404735E-5
86-87	1.434450281264761	7.156864148404735E-5	7.156864148404735E-5
88-89	1.579555701873667	7.156864148404735E-5	7.156864148404735E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	4435	0.0	90.30501	9
GTTCGGA	800	0.0	90.095825	2
ATTCGGA	950	0.0	88.348816	2
AAGCGGC	1305	0.0	88.25606	9
CGGGAGA	5805	0.0	88.205315	4
AGAGCGG	4815	0.0	85.04841	8
ATCGGGA	7310	0.0	83.74521	2
ATCCGGA	1100	0.0	81.9053	2
TCGGGAG	6480	0.0	80.626884	3
TCGGAGA	1420	0.0	78.79468	4
TTGGGAG	2965	0.0	78.03122	3
GTCCGGA	505	0.0	76.996956	2
CGGAAAG	2190	0.0	76.85241	5
CGGAGAG	2315	0.0	76.79864	5
GGAGAGC	5795	0.0	75.99431	6
GATCCGG	1275	0.0	74.38919	1
GGTTCGG	1155	0.0	73.90614	1
AAGGGGC	3495	0.0	73.77351	9
GGAAAGC	1785	0.0	73.29758	6
TCGGGAA	1955	0.0	73.23749	3
>>END_MODULE