FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_50330665-1.342000000356cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_50330665-1.342000000356cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794520
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT258190.9239153772383093Illumina Single End PCR Primer 1 (97% over 34bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA213280.7632079927858809No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC198660.7108913158610423No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC111650.3995319410846943No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT96590.3456407540472067No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT95590.3420623219730043No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC76020.272032406280864No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT65220.2333853398794784No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT57190.20465053032363342No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC43840.15687846213303178No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA41690.1491848331734967No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT39110.1399524784220546No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA33770.12084365114581395No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC30490.10910639394243017No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC29420.10527747162303366No Hit
TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA28590.10230737300144568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC44350.090.305019
GTTCGGA8000.090.0958252
ATTCGGA9500.088.3488162
AAGCGGC13050.088.256069
CGGGAGA58050.088.2053154
AGAGCGG48150.085.048418
ATCGGGA73100.083.745212
ATCCGGA11000.081.90532
TCGGGAG64800.080.6268843
TCGGAGA14200.078.794684
TTGGGAG29650.078.031223
GTCCGGA5050.076.9969562
CGGAAAG21900.076.852415
CGGAGAG23150.076.798645
GGAGAGC57950.075.994316
GATCCGG12750.074.389191
GGTTCGG11550.073.906141
AAGGGGC34950.073.773519
GGAAAGC17850.073.297586
TCGGGAA19550.073.237493
Produced by FastQC (version 0.11.2)