##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3312039 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27536088796056 33.0 33.0 33.0 33.0 33.0 2 32.2945161575694 33.0 33.0 33.0 33.0 33.0 3 32.27585997628651 33.0 33.0 33.0 33.0 33.0 4 36.10396435549219 37.0 37.0 37.0 33.0 37.0 5 36.1198234682623 37.0 37.0 37.0 33.0 37.0 6 36.024673320573825 37.0 37.0 37.0 33.0 37.0 7 35.9915052328792 37.0 37.0 37.0 33.0 37.0 8 35.91718455006116 37.0 37.0 37.0 33.0 37.0 9 35.908499869717716 37.0 37.0 37.0 33.0 37.0 10-11 35.8030951628287 37.0 37.0 37.0 33.0 37.0 12-13 35.79223282092995 37.0 37.0 37.0 33.0 37.0 14-15 37.57741500024608 40.0 37.0 40.0 33.0 40.0 16-17 37.42268992001604 40.0 37.0 40.0 33.0 40.0 18-19 37.197443931064825 40.0 37.0 40.0 33.0 40.0 20-21 37.1405294442487 40.0 37.0 40.0 33.0 40.0 22-23 36.96483616285919 40.0 37.0 40.0 33.0 40.0 24-25 36.76668194426455 40.0 37.0 40.0 33.0 40.0 26-27 36.664841356034756 40.0 37.0 40.0 33.0 40.0 28-29 36.58800983321754 38.5 37.0 40.0 33.0 40.0 30-31 36.60100349059899 40.0 37.0 40.0 33.0 40.0 32-33 36.50368821140089 37.0 37.0 40.0 33.0 40.0 34-35 36.4397757695486 37.0 37.0 40.0 33.0 40.0 36-37 36.348649578099774 37.0 37.0 40.0 33.0 40.0 38-39 36.246514005420835 37.0 37.0 40.0 33.0 40.0 40-41 36.07182886433402 37.0 37.0 40.0 33.0 40.0 42-43 35.9852948591487 37.0 37.0 40.0 33.0 40.0 44-45 35.852742374108516 37.0 37.0 40.0 27.0 40.0 46-47 35.75090903217021 37.0 37.0 40.0 27.0 40.0 48-49 35.60824872533204 37.0 37.0 40.0 27.0 40.0 50-51 34.28970114784276 37.0 35.0 38.5 24.5 38.5 52-53 34.75579499516763 37.0 35.0 38.5 27.0 40.0 54-55 35.52115132098385 37.0 37.0 40.0 27.0 40.0 56-57 35.46259464336018 37.0 37.0 40.0 27.0 40.0 58-59 35.27641160022573 37.0 37.0 40.0 27.0 40.0 60-61 35.032116016749804 37.0 37.0 40.0 27.0 40.0 62-63 34.74526870003645 37.0 33.0 40.0 27.0 40.0 64-65 34.52287533449939 37.0 33.0 38.5 27.0 40.0 66-67 34.243477356395864 37.0 33.0 37.0 27.0 40.0 68-69 33.975171789945705 37.0 33.0 37.0 27.0 40.0 70-71 33.65550918935435 37.0 33.0 37.0 24.5 40.0 72-73 33.3385829695846 37.0 33.0 37.0 22.0 38.5 74-75 33.048634089151726 37.0 33.0 37.0 22.0 37.0 76-77 32.79352190599205 37.0 33.0 37.0 22.0 37.0 78-79 32.56400528496192 37.0 33.0 37.0 22.0 37.0 80-81 32.30385753307857 37.0 33.0 37.0 22.0 37.0 82-83 32.05968226823416 37.0 33.0 37.0 22.0 37.0 84-85 31.861065645664198 37.0 33.0 37.0 15.0 37.0 86-87 31.659596399680076 35.0 33.0 37.0 15.0 37.0 88-89 31.453422951843258 33.0 33.0 37.0 15.0 37.0 90-91 31.25518811825585 33.0 33.0 37.0 15.0 37.0 92-93 31.08255458344542 33.0 33.0 37.0 15.0 37.0 94-95 30.89616426618165 33.0 33.0 37.0 15.0 37.0 96-97 30.67256695950742 33.0 30.0 37.0 15.0 37.0 98-99 30.471642845993056 33.0 27.0 37.0 15.0 37.0 100-101 29.254924081509913 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16263706520216914 2306 2 0.1673885171966205 2306 3 0.1680001214368403 2306 4 0.1627583318739596 2306 5 0.2397395681394272 2306 6 0.22444698083184278 2306 7 0.2306126974870324 2306 8 0.28556496640464957 2306 9 0.29133543973530607 2306 10-11 0.2638165423145935 2306 12-13 0.3131403355983977 2306 14-15 0.42248512910310154 2306 16-17 0.49226995574827725 2306 18-19 0.5330207824559565 2306 20-21 0.5017538797604999 2306 22-23 0.6254166915772856 2306 24-25 0.5710709653515522 2306 26-27 0.6296945011257051 2306 28-29 0.5015132381090055 2306 30-31 0.42080496906823583 2306 32-33 0.46348595399856407 2306 34-35 0.46550511336643297 2306 36-37 0.4328283497225627 2306 38-39 0.4654533989256606 2306 40-41 0.5412700137151418 2306 42-43 0.5744951485732201 2306 44-45 0.588441784948067 2306 46-47 0.5048361349795414 2306 48-49 0.558189091167165 2306 50-51 0.5732303001006827 2306 52-53 0.6003179316639731 2306 54-55 0.6802643456054724 2306 56-57 0.5543006713084466 2306 58-59 0.7461447863657398 2306 60-61 0.6787544344978684 2306 62-63 0.7585733297433634 2306 64-65 0.7315508510703879 2306 66-67 0.8630262043390076 2306 68-69 0.858544139168437 2306 70-71 0.8988999552435146 2306 72-73 0.8698241376690063 2306 74-75 0.8483349156431856 2306 76-77 0.8842037633545345 2306 78-79 0.8607206379395791 2306 80-81 0.9356924157900508 2306 82-83 0.9024730947706416 2306 84-85 0.9683874569354813 2306 86-87 0.9827264576012027 2306 88-89 0.9290452183982971 2306 90-91 0.9912561184201287 2306 92-93 1.0608144013934613 2306 94-95 1.2767378652181556 2306 96-97 1.1006728051679495 2306 98-99 1.1172509246660098 2306 100-101 1.1240022597076056 2307 1 0.18455195330909646 2307 2 0.18512097088069623 2307 3 0.15843992448691324 2307 4 0.25237475861175795 2307 5 0.16885183672163606 2307 6 0.2908083276275235 2307 7 0.35773264465628785 2307 8 0.22762756695950515 2307 9 0.30807084248885275 2307 10-11 0.40331343624198013 2307 12-13 0.35289005315458155 2307 14-15 0.49344772027014017 2307 16-17 0.5850313012490815 2307 18-19 0.6911618004190601 2307 20-21 0.6669640859363852 2307 22-23 0.6870521626352897 2307 24-25 0.6454147893008368 2307 26-27 0.6441003494121063 2307 28-29 0.548103922804593 2307 30-31 0.4973306512226401 2307 32-33 0.4451194180060014 2307 34-35 0.4569719112467254 2307 36-37 0.3498764639043159 2307 38-39 0.45371458094896155 2307 40-41 0.5375844861683774 2307 42-43 0.4829226568199303 2307 44-45 0.3787414181757569 2307 46-47 0.3849079841529246 2307 48-49 0.47467798278967166 2307 50-51 0.4248914581688652 2307 52-53 0.3470075121580294 2307 54-55 0.5885477994157995 2307 56-57 0.6239186195200617 2307 58-59 0.7014786041967724 2307 60-61 0.6486154948032592 2307 62-63 0.5819132237581428 2307 64-65 0.5223562934146244 2307 66-67 0.6958603377949188 2307 68-69 0.6536004013280845 2307 70-71 0.5294286512109636 2307 72-73 0.6429424468260123 2307 74-75 0.6987966991342276 2307 76-77 0.7599163472379189 2307 78-79 0.5707030438752696 2307 80-81 0.763883626203409 2307 82-83 0.741790082511443 2307 84-85 0.713461280787655 2307 86-87 0.8576961090542028 2307 88-89 1.0444558868432559 2307 90-91 0.9331657622981346 2307 92-93 0.9359455071164682 2307 94-95 1.043035844247708 2307 96-97 1.0260598907686536 2307 98-99 0.9015172667515969 2307 100-101 0.6528212399316828 2201 1 0.32996375782722254 2201 2 0.27594631532254965 2201 3 0.2891824900816289 2201 4 0.3037452951847044 2201 5 0.31806273333340584 2201 6 0.41128596909562276 2201 7 0.41124243017461737 2201 8 0.3741250846064119 2201 9 0.3998535523536191 2201 10-11 0.27867552138965124 2201 12-13 0.3391394945709081 2201 14-15 0.5842497451992443 2201 16-17 0.5142616416344268 2201 18-19 0.7643037233352317 2201 20-21 0.6112217734216472 2201 22-23 0.5642037040370056 2201 24-25 0.5912806193091811 2201 26-27 0.6496847400172001 2201 28-29 0.6312049046103141 2201 30-31 0.5695506399135866 2201 32-33 0.45594437198159454 2201 34-35 0.48912066153777545 2201 36-37 0.5059111199541633 2201 38-39 0.5555051579857846 2201 40-41 0.5764689602175537 2201 42-43 0.46909635501265967 2201 44-45 0.4097652279405892 2201 46-47 0.4255032788898774 2201 48-49 0.426339525189249 2201 50-51 0.49041332355633926 2201 52-53 0.39256353611241934 2201 54-55 0.5578311202879505 2201 56-57 0.6755500092473312 2201 58-59 0.629046209091193 2201 60-61 0.7906040310030988 2201 62-63 0.7228973939667256 2201 64-65 0.6383341727778955 2201 66-67 0.5998099763244014 2201 68-69 0.610806022306825 2201 70-71 0.5457514062830455 2201 72-73 0.691923546812852 2201 74-75 0.7389532616428127 2201 76-77 0.7373929518011693 2201 78-79 0.6808546604778911 2201 80-81 0.769503628777592 2201 82-83 0.7659124011290288 2201 84-85 0.8280528241561669 2201 86-87 0.869277229865915 2201 88-89 0.8755813986071352 2201 90-91 0.9902616911812139 2201 92-93 0.7814352964237621 2201 94-95 0.9515966000746978 2201 96-97 1.0266041935043795 2201 98-99 0.8446944834791736 2201 100-101 0.8611073316681122 1101 1 0.1472074182971923 1101 2 0.07899585883937732 1101 3 0.1394890867087213 1101 4 0.16725717392463935 1101 5 0.20359498091451655 1101 6 0.24105602740796428 1101 7 0.2339125386804426 1101 8 0.2614545828797077 1101 9 0.24735093280869336 1101 10-11 0.2589884558119806 1101 12-13 0.13033921145188287 1101 14-15 0.256948304391706 1101 16-17 0.22366389964745537 1101 18-19 0.40033443619841336 1101 20-21 0.3769170688808714 1101 22-23 0.4180579625034042 1101 24-25 0.4081755853881006 1101 26-27 0.35184999918445214 1101 28-29 0.5269597383357123 1101 30-31 0.533551269066983 1101 32-33 0.6104431657187419 1101 34-35 0.4337621019289628 1101 36-37 0.35444764439052534 1101 38-39 0.49137430333178855 1101 40-41 0.3701517512530401 1101 42-43 0.40817603312667217 1101 44-45 0.19048213898246757 1101 46-47 0.18639434051683423 1101 48-49 0.1565194584342251 1101 50-51 0.30954155288275587 1101 52-53 0.3845134952868392 1101 54-55 0.568416279207014 1101 56-57 0.5172774516693863 1101 58-59 0.409498852576327 1101 60-61 0.4756381602617381 1101 62-63 0.5471230379815566 1101 64-65 0.47367061949531575 1101 66-67 0.4937525244419092 1101 68-69 0.5767542513094099 1101 70-71 0.614928788218478 1101 72-73 0.6176857592122644 1101 74-75 0.7180905813105554 1101 76-77 0.6094862978100934 1101 78-79 0.654099449723077 1101 80-81 0.650277325539534 1101 82-83 0.6718308378678799 1101 84-85 0.6262865685209604 1101 86-87 0.7624859584440458 1101 88-89 0.7277407594613017 1101 90-91 0.5119235351095348 1101 92-93 0.8135498763597901 1101 94-95 0.9139185230635469 1101 96-97 0.9403442560033675 1101 98-99 0.7711743239702784 1101 100-101 0.5183118319653452 2304 1 0.11960167100583874 2304 2 0.08191336472221877 2304 3 0.06234885088670694 2304 4 0.17287016042030245 2304 5 0.123389222362988 2304 6 0.2302250687007259 2304 7 0.13581989769061664 2304 8 0.17311561643079898 2304 9 0.14833038661744524 2304 10-11 0.13406753434203011 2304 12-13 0.16872029762311058 2304 14-15 0.18130291764396844 2304 16-17 0.23677360646512824 2304 18-19 0.3513930744797378 2304 20-21 0.4120641837025971 2304 22-23 0.3883144463374819 2304 24-25 0.2757627866831456 2304 26-27 0.27459307523484 2304 28-29 0.27766773711767456 2304 30-31 0.17180293887257392 2304 32-33 0.12127843780034908 2304 34-35 0.12455982797852982 2304 36-37 0.04105511642624293 2304 38-39 0.23843152258861977 2304 40-41 0.36609188222222144 2304 42-43 0.29344133440603315 2304 44-45 0.33737425437627877 2304 46-47 0.20957759263765752 2304 48-49 0.20703270457988765 2304 50-51 0.24315327291115096 2304 52-53 0.24394728393088627 2304 54-55 0.3183421194372045 2304 56-57 0.4189109492439087 2304 58-59 0.36790339442332964 2304 60-61 0.526390836521351 2304 62-63 0.4335102706355869 2304 64-65 0.37443985305863947 2304 66-67 0.44274354293705187 2304 68-69 0.6505766698380953 2304 70-71 0.429160986392759 2304 72-73 0.5081406552402683 2304 74-75 0.5729260101871105 2304 76-77 0.6623846129606648 2304 78-79 0.4923012962710729 2304 80-81 0.4783758115300003 2304 82-83 0.5964973005195091 2304 84-85 0.6688392174171298 2304 86-87 0.7721450414418456 2304 88-89 0.8819165750220215 2304 90-91 0.7578168243465662 2304 92-93 0.8201554686723576 2304 94-95 0.8612304281584144 2304 96-97 0.8490980752315131 2304 98-99 0.9260850023112113 2304 100-101 0.7965779795741632 2202 1 0.1258320339726069 2202 2 0.1416932996247411 2202 3 0.1349908049839712 2202 4 0.2188285562464145 2202 5 0.08873898826620064 2202 6 0.19429058756368534 2202 7 0.2332529783739119 2202 8 0.2548499309686889 2202 9 0.29545459638467975 2202 10-11 0.25065245809630454 2202 12-13 0.302962532136938 2202 14-15 0.33602264769686485 2202 16-17 0.4769290247929945 2202 18-19 0.4473265998980622 2202 20-21 0.4543847393661551 2202 22-23 0.47667688746032155 2202 24-25 0.4954889026748148 2202 26-27 0.47907425572123685 2202 28-29 0.4478220531108619 2202 30-31 0.3657052361814692 2202 32-33 0.3037486286074156 2202 34-35 0.265772292940035 2202 36-37 0.2560293826134554 2202 38-39 0.30959455625707477 2202 40-41 0.42982637487133246 2202 42-43 0.34692791578928706 2202 44-45 0.352110106752491 2202 46-47 0.42267327420086787 2202 48-49 0.38616860452481916 2202 50-51 0.4939072589641782 2202 52-53 0.47961937683543 2202 54-55 0.6404853078234822 2202 56-57 0.6088789163758719 2202 58-59 0.6866152267641965 2202 60-61 0.6234636221047722 2202 62-63 0.7000550650637436 2202 64-65 0.6435159445600149 2202 66-67 0.7374314030285234 2202 68-69 0.6508451942192366 2202 70-71 0.6924251133817094 2202 72-73 0.7047457088202478 2202 74-75 0.6581902392641936 2202 76-77 0.6609485912701913 2202 78-79 0.7791942112457733 2202 80-81 0.7806887866063761 2202 82-83 0.9897846525595853 2202 84-85 0.9286818985122594 2202 86-87 0.8758584431163143 2202 88-89 0.9500578269918343 2202 90-91 0.9367934830887243 2202 92-93 0.7791748198044992 2202 94-95 0.8920067432762515 2202 96-97 1.0395975251502207 2202 98-99 1.0676828771402924 2202 100-101 1.082878864808631 1102 1 0.012111108479892607 1102 2 -0.04795166044172561 1102 3 -0.019456510457594334 1102 4 0.04367672176481108 1102 5 0.028539890529259537 1102 6 0.07796450268030242 1102 7 0.040639014759833 1102 8 0.06522761680410838 1102 9 0.04923677652578107 1102 10-11 0.1101228107106138 1102 12-13 0.12880126714692608 1102 14-15 0.14880136527957433 1102 16-17 0.12599265449324548 1102 18-19 0.21536896736103728 1102 20-21 0.2967893290362582 1102 22-23 0.17889478934770153 1102 24-25 0.14422668300906594 1102 26-27 0.23572510548353875 1102 28-29 0.24993052496792956 1102 30-31 0.1488255596454593 1102 32-33 0.21393145903817867 1102 34-35 0.19367868685946377 1102 36-37 0.22033742827463954 1102 38-39 0.18344587433663406 1102 40-41 0.22234015553168973 1102 42-43 0.13340555711292978 1102 44-45 0.16523102907478204 1102 46-47 0.22573941608650472 1102 48-49 0.18395019939517 1102 50-51 0.11165104021080907 1102 52-53 0.27395086855370465 1102 54-55 0.3550186173031378 1102 56-57 0.5102946375132333 1102 58-59 0.49349669987508094 1102 60-61 0.5185869906877016 1102 62-63 0.5207833748818231 1102 64-65 0.6031057794688763 1102 66-67 0.5082171956942219 1102 68-69 0.4650328014255649 1102 70-71 0.5590584905726033 1102 72-73 0.5079148266541722 1102 74-75 0.49613050390420455 1102 76-77 0.6407082503134944 1102 78-79 0.5664332581149907 1102 80-81 0.5869049734441205 1102 82-83 0.6935263720226601 1102 84-85 0.6675806886926878 1102 86-87 0.8990564646696697 1102 88-89 1.0102331465317995 1102 90-91 0.9985455235473957 1102 92-93 0.9187076071703011 1102 94-95 1.0942289227228876 1102 96-97 0.8460678493313374 1102 98-99 0.7891171477875041 1102 100-101 0.8806815958540746 2305 1 0.12380612284169956 2305 2 0.08986459128661295 2305 3 0.08841260731994538 2305 4 0.15298267388403275 2305 5 0.07358892901900305 2305 6 0.11313457756743617 2305 7 0.2552487586987837 2305 8 0.15509241754593717 2305 9 0.17820717906863592 2305 10-11 0.17328870657985362 2305 12-13 0.20441994506691685 2305 14-15 0.21324624206898335 2305 16-17 0.3116370082402753 2305 18-19 0.399387395233596 2305 20-21 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68-69 -1.6134641834494872 1213 70-71 -1.606579090777096 1213 72-73 -1.6878134913888019 1213 74-75 -1.7721280354347186 1213 76-77 -1.564973900822448 1213 78-79 -1.5446527466251183 1213 80-81 -1.7056532222961849 1213 82-83 -1.9275295289830758 1213 84-85 -1.9979121401736393 1213 86-87 -2.1258733033995725 1213 88-89 -2.2583723144196384 1213 90-91 -2.265141847629181 1213 92-93 -2.364003495872435 1213 94-95 -2.53358886890846 1213 96-97 -2.5735119074170463 1213 98-99 -2.8673197302895197 1213 100-101 -2.5928473072194542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1293.0 3 214.0 4 180.0 5 299.0 6 539.0 7 1120.0 8 3322.0 9 3756.0 10 5101.0 11 5872.0 12 6120.0 13 8164.0 14 11401.0 15 14811.0 16 16201.0 17 15841.0 18 15887.0 19 16734.0 20 18248.0 21 20096.0 22 21908.0 23 24021.0 24 27282.0 25 30626.0 26 33624.0 27 39190.0 28 45506.0 29 53803.0 30 63793.0 31 79265.0 32 102008.0 33 136898.0 34 198145.0 35 323673.0 36 636327.0 37 994298.0 38 336278.0 39 195.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.007793002934854 32.51084613462577 19.121655182761376 15.359705679677996 2 30.353313875537875 26.30940798931273 30.308571836535158 13.028706298614237 3 33.971938394390335 24.170366862216028 24.702094186685827 17.155600556707803 4 29.44560123754179 24.135482397843074 29.122999617426597 17.29591674718854 5 29.28275067764612 22.514969914992538 30.307552434777506 17.89472697258384 6 26.86084651363952 23.215596489821966 31.827781701163055 18.095775295375464 7 30.89703156316791 23.11770071696129 25.868959926903994 20.116307792966808 8 27.246108953239123 28.48499853236491 24.97954228527259 19.289350229123382 9 25.23270492725024 26.374615634820735 28.868016584987178 19.52466285294185 10-11 20.24607618778205 31.576593975045725 32.85466632333687 15.322663513835344 12-13 20.434790759384857 30.850605136745756 32.81530773577837 15.899296368091015 14-15 20.516898842737163 30.819778728879093 32.97166728436525 15.69165514401849 16-17 20.157869727473933 30.898192207685433 32.92607173494982 16.01786632989081 18-19 19.48006507008274 31.28876156602886 32.58624861723051 16.64492474665789 20-21 19.453207070909343 31.564527934269144 32.08446196281255 16.897803032008973 22-23 19.55230455924525 31.01820751045638 32.10375144991329 17.325736480385082 24-25 19.398630811221977 30.737446258923352 32.33063171218369 17.533291217670985 26-27 19.4574799822502 31.154918528099056 32.14450036271997 17.24310112693078 28-29 19.227329268235383 31.41919772845184 31.937477255645106 17.41599574766767 30-31 18.68323790602894 32.4880515953733 31.73239266717512 17.096317831422645 32-33 18.40575403028442 32.90623917989202 31.830507545669846 16.85749924415372 34-35 18.793949501853906 32.36877499096744 32.26983324666719 16.567442260511463 36-37 18.809587323484358 31.909570942505795 32.700233239700346 16.5806084943095 38-39 19.100019418069834 31.758601583920747 32.10971724442746 17.031661753581968 40-41 19.00649387531002 31.760464984191294 31.50269410628751 17.730347034211174 42-43 19.27574764825042 31.32050267430403 31.45747940689681 17.946270270548744 44-45 18.912521781142775 31.25072414490319 31.74442247503451 18.09233159891952 46-47 19.072375939685607 31.523944940452747 31.60446128731626 17.799217832545388 48-49 18.987181470035637 32.067486899486916 31.08146244640768 17.863869184069763 50-51 19.558051261011673 31.949699522490288 30.218026105318785 18.274223111179253 52-53 19.680113994175766 31.78025459325418 30.34535926844244 18.194272144127613 54-55 20.054517983240522 31.849719019439636 30.21122613664452 17.884536860675322 56-57 20.19065118452614 31.88011666895147 30.07521678505995 17.854015361462437 58-59 19.9937413815249 32.45882822667226 29.958910018878854 17.588520372923995 60-61 19.79859831672627 32.350497439625606 29.780628678404042 18.07027556524408 62-63 19.625802652991318 32.208672665381876 30.19326063760352 17.97226404402329 64-65 19.46883075011218 31.807459248901953 30.62252482425883 18.101185176727036 66-67 19.938321286062678 31.41052572909892 30.315184831676966 18.335968153161435 68-69 20.327648648464468 31.48487562874664 30.377933236060432 17.809542486728457 70-71 20.04041419630034 31.79116353970214 30.436023600437 17.732398663560524 72-73 20.10359965971445 31.743164046975636 30.143052859172258 18.01018343413765 74-75 20.496662119255234 31.334364954750484 29.748595273821294 18.42037765217299 76-77 20.663997925243045 31.09280071144906 29.30441290085814 18.93878846244976 78-79 20.488900510606946 31.282113555120734 29.566296761916472 18.662689172355847 80-81 20.32721424969677 31.62692517940075 29.184561314247727 18.86129925665475 82-83 19.976147539915914 31.7593506702408 29.06339259289328 19.20110919695001 84-85 20.103716893079422 31.703218043873772 29.632878730691097 18.56018633235571 86-87 20.46615233422229 31.381705178496055 29.902377196073886 18.24976529120777 88-89 20.902062531042027 31.273988224970946 29.780002444737054 18.043946799249976 90-91 20.972300683509296 31.26578712615616 29.239340329471773 18.52257186086277 92-93 20.766302473887134 31.35524627949296 29.163048134715236 18.715403111904667 94-95 20.632013152846113 31.147718466345193 29.28145098011901 18.938817400689683 96-97 20.76975471992052 31.57498634018976 28.958282962231095 18.69697597765862 98-99 21.412197094285794 31.30237720979959 28.648331046681026 18.637094649233592 100-101 21.825627950605348 31.270654426737153 28.071923366871083 18.83179425578642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 133203.0 1 76777.5 2 14459.5 3 8335.5 4 7284.5 5 6218.5 6 6429.0 7 7271.5 8 7675.0 9 7573.0 10 7194.5 11 6786.0 12 6444.0 13 6088.5 14 5861.5 15 5820.0 16 5844.5 17 5868.0 18 5818.5 19 5785.0 20 5911.0 21 6157.0 22 6571.0 23 7259.5 24 8321.5 25 9777.0 26 11881.0 27 14659.0 28 17941.5 29 21585.0 30 26118.5 31 31789.0 32 40414.5 33 55054.0 34 74489.0 35 95572.0 36 123682.0 37 160891.0 38 189461.0 39 193952.0 40 192800.5 41 192790.0 42 185776.0 43 180002.0 44 169539.0 45 156548.0 46 152284.0 47 141193.5 48 117346.0 49 94449.0 50 75273.0 51 60425.0 52 49016.5 53 38451.5 54 30468.5 55 25560.0 56 20461.5 57 15052.5 58 10907.5 59 7799.5 60 5651.0 61 4351.5 62 3337.5 63 2546.0 64 2040.0 65 1556.5 66 1069.0 67 746.5 68 583.0 69 488.5 70 379.5 71 290.0 72 231.5 73 188.5 74 153.5 75 114.5 76 98.0 77 86.0 78 76.0 79 60.0 80 44.5 81 39.5 82 30.0 83 23.5 84 19.5 85 15.0 86 15.0 87 8.5 88 3.5 89 5.0 90 4.5 91 2.5 92 2.5 93 4.0 94 2.5 95 2.0 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1887960860364265 2 0.19392887583751278 3 0.16476255261486958 4 0.1655777604068068 5 0.17333733087080194 6 0.1707105502078931 7 0.13912879649062104 8 0.12065075320671043 9 0.12037901727606469 10-11 0.09864014282440516 12-13 0.14402004324224443 14-15 0.12261329048359636 16-17 0.11488391290078409 18-19 0.11815984050912445 20-21 0.10985679818383781 22-23 0.110249305639215 24-25 0.46984652052708314 26-27 0.31986338325122377 28-29 0.39684013382692657 30-31 0.25858693089060847 32-33 0.28530763073744 34-35 0.30482732842215926 36-37 0.2846584837920085 38-39 0.33181976419963655 40-41 0.3276380501558104 42-43 0.36860978992095206 44-45 0.3240149044138671 46-47 0.346221768523861 48-49 0.34146638973756044 50-51 0.3560344549082906 52-53 0.34780689478596116 54-55 0.32436212254747 56-57 0.33956423822304027 58-59 0.3318499570808194 60-61 0.3043593387638249 62-63 0.3119528483813144 64-65 0.3133870102375002 66-67 0.3242111581415557 68-69 0.31193775194072293 70-71 0.30137024352672176 72-73 0.28739093953905737 74-75 0.2957090783049354 76-77 0.28633418869765725 78-79 0.29566378898316115 80-81 0.3041630850361363 82-83 0.32946471946737343 84-85 0.3273210249033903 86-87 0.3202709871471924 88-89 0.3342502911348568 90-91 0.3228826713695098 92-93 0.30707669807028243 94-95 0.30011723895763304 96-97 0.2858511025987315 98-99 0.31005069686679415 100-101 0.3135832639651888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3312039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.12280120772294 #Duplication Level Percentage of deduplicated Percentage of total 1 82.04487621326086 36.20049763269945 2 8.592061285968281 7.582116241707014 3 2.9910832055827377 3.9592490902705744 4 1.6476911393290339 2.908029943693659 5 0.8749912569958619 1.930353264546201 6 0.6806264231176569 1.8018686618366337 7 0.4885764865398646 1.5090154233256317 8 0.310196345939096 1.0949385365786228 9 0.28161722863294547 1.1183166896077181 >10 1.7838494300480967 14.722598889321253 >50 0.17036939020752387 5.2133915587022654 >100 0.122535466180886 10.936766779933027 >500 0.00983498134906169 2.936840800426381 >1k 0.001560629545023501 0.8987598557953258 >5k 0.0 0.0 >10k+ 1.305173030379178E-4 7.18725663155631 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140093 4.229811303550472 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109517 3.3066337685033296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2077152473144186E-4 0.0 0.0 2 2.1135016828002329E-4 0.0 0.0 3 2.1135016828002329E-4 0.0 0.0 4 3.3212169301146516E-4 0.0 0.0 5 3.6231457419432563E-4 0.0 0.0 6 3.925074553771861E-4 0.0 0.0 7 5.736647424743489E-4 0.0 0.0 8 6.944362672057908E-4 0.0 0.0 9 0.001026557960217256 0.0 0.0 10-11 0.0023399482916716865 1.5096440591430233E-5 0.0 12-13 0.007699184701629419 3.0192881182860466E-5 0.0 14-15 0.015836166180410314 6.038576236572093E-5 0.0 16-17 0.02893987661377176 6.038576236572093E-5 0.0 18-19 0.04056413586917304 6.038576236572093E-5 0.0 20-21 0.04722166616999377 6.038576236572093E-5 0.0 22-23 0.053713135624308773 6.038576236572093E-5 0.0 24-25 0.06198598506841255 6.038576236572093E-5 0.0 26-27 0.07134577823509929 6.038576236572093E-5 0.0 28-29 0.08156606851549755 6.038576236572093E-5 0.0 30-31 0.09323561709267313 6.038576236572093E-5 0.0 32-33 0.10778858582281187 6.038576236572093E-5 0.0 34-35 0.1236851377655879 6.038576236572093E-5 0.0 36-37 0.1405629583468069 6.038576236572093E-5 0.0 38-39 0.15946370196727755 6.038576236572093E-5 0.0 40-41 0.1789230138896311 6.038576236572093E-5 0.0 42-43 0.20090343139075356 6.038576236572093E-5 0.0 44-45 0.22347261007494174 6.038576236572093E-5 0.0 46-47 0.24729479332821866 6.038576236572093E-5 0.0 48-49 0.27310970673956436 6.038576236572093E-5 0.0 50-51 0.3018080403038732 6.038576236572093E-5 0.0 52-53 0.3316989926749051 6.038576236572093E-5 0.0 54-55 0.3640204719811572 6.038576236572093E-5 0.0 56-57 0.39694580891106657 6.038576236572093E-5 0.0 58-59 0.43340371293937063 6.038576236572093E-5 0.0 60-61 0.4704050888289661 9.057864354858141E-5 0.0 62-63 0.5095048699607704 9.057864354858141E-5 0.0 64-65 0.5519107715820979 9.057864354858141E-5 0.0 66-67 0.5987852196184889 9.057864354858141E-5 0.0 68-69 0.6497206101739744 9.057864354858141E-5 0.0 70-71 0.705713308327589 9.057864354858141E-5 0.0 72-73 0.7672313037376673 9.057864354858141E-5 0.0 74-75 0.8360559763939978 9.057864354858141E-5 0.0 76-77 0.914240442217015 9.057864354858141E-5 0.0 78-79 1.0015129652760733 9.057864354858141E-5 0.0 80-81 1.1014514019913413 9.057864354858141E-5 0.0 82-83 1.2202603894458972 9.057864354858141E-5 0.0 84-85 1.3478856982058485 9.057864354858141E-5 0.0 86-87 1.4953024405811646 9.057864354858141E-5 0.0 88-89 1.6671150309522322 9.057864354858141E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACC 30 1.3096223E-4 63.25458 1 CCGAGCG 30 1.3096223E-4 63.25458 1 CGGGTCG 40 5.422819E-4 47.440933 1 CGCGAGC 50 2.8490294E-5 47.440933 1 CGGACGG 45 9.6868316E-4 42.169724 1 AGTCGGC 80 2.2865424E-7 41.510815 1 CGCGGAG 175 0.0 40.66366 1 CGTGCGG 70 4.3496857E-6 40.66366 1 GATCCGG 70 4.3496857E-6 40.66366 1 CGAGCGG 85 3.679197E-7 39.069004 1 TCGTCGC 50 0.0016270103 37.94873 2 CGACGGG 145 1.6370905E-11 35.989677 1 CCGGCTA 80 1.0874521E-5 35.5807 1 ATGCCGG 55 0.0025961145 34.5025 1 TGCCGGC 55 0.0025974626 34.498848 2 CGCAGCC 180 0.0 34.262897 1 AGGCCGT 70 2.0692307E-4 33.886383 1 CGGACCG 70 2.0692307E-4 33.886383 1 CGAGGCG 170 3.6379788E-12 33.48772 1 CGCGTTG 85 1.6503345E-5 33.478096 6 >>END_MODULE