FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312039
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400934.229811303550472No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1095173.3066337685033296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC301.3096223E-463.254581
CCGAGCG301.3096223E-463.254581
CGGGTCG405.422819E-447.4409331
CGCGAGC502.8490294E-547.4409331
CGGACGG459.6868316E-442.1697241
AGTCGGC802.2865424E-741.5108151
CGCGGAG1750.040.663661
CGTGCGG704.3496857E-640.663661
GATCCGG704.3496857E-640.663661
CGAGCGG853.679197E-739.0690041
TCGTCGC500.001627010337.948732
CGACGGG1451.6370905E-1135.9896771
CCGGCTA801.0874521E-535.58071
ATGCCGG550.002596114534.50251
TGCCGGC550.002597462634.4988482
CGCAGCC1800.034.2628971
AGGCCGT702.0692307E-433.8863831
CGGACCG702.0692307E-433.8863831
CGAGGCG1703.6379788E-1233.487721
CGCGTTG851.6503345E-533.4780966
Produced by FastQC (version 0.11.2)