FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5903582
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC201320.341013303448652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105850.1792979245481811No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95550.16185088984958623No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG90620.1535000276103559No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA77590.13142868177320144No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT68060.11528593995984134Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG3350.049.553341
CGCGGAG4550.048.9932371
CGCAGCC4650.044.8796461
CCGGGGC1350.042.1596641
CGAGGGG10000.041.7380681
CGACGGG3400.040.454681
CGGAAAG3450.039.860945
ATTACGG2550.039.0596921
GGACGGG8050.038.8864021
TGTACCG1600.038.532972
ATGACCG1850.038.456451
CGGGAGA9550.038.2348754
GTTGACG2000.037.94371
GGTACGG1500.037.94371
ACGCGTG500.001628173537.94371
CGGACCG1750.037.94371
TCAGCCG3450.037.115372
ACTGGCG9000.036.8862652
CTGCGGA1700.036.2697141
CGGCAGC2800.035.572221
Produced by FastQC (version 0.11.2)