FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3840504
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT48620.12659796735011863Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGC850.061.361661
GTGCGAC250.005057548656.898991
GAGCGGC4250.054.645569
CGGGAGA6350.050.021474
CGACGGG1500.047.4158251
CGAGGCG1400.047.4158251
CGGACCG405.4370984E-447.4158251
ACGTGCG405.441296E-447.4084053
GGTCCGG1052.1827873E-1145.157931
CGAGGGG4500.042.14741
AACGCCG901.216722E-842.14741
CGAGCGG901.216722E-842.14741
CGGCCCG459.7197836E-442.14083
AGAGCGG5300.042.0310028
CGGACGG500.001630431537.932661
CCGCCGA651.3439379E-436.4680024
GAGAGCG7200.036.205787
GATCGGG13500.035.825291
CCGGCGA801.0914495E-535.561871
GATGCGC550.00260291534.4842381
Produced by FastQC (version 0.11.2)