FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995253
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT190750.6368410281201622Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC55040.18375743217684784No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54300.18128685623551666No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG52430.17504364405945008No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG45710.15260814361925354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41150.13738405403483445No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA36160.12072435951153374No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA32510.10853841061172463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.10329678327673823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC32100.089.360859
AGAGCGG34100.087.317688
CGGGAGA41200.086.9086154
GAGAGCG38750.082.100877
ATTCGGA7000.081.9922942
TCGGGAG45600.081.018783
AAGCGGC9350.080.120289
GTTCGGA5450.080.0713352
CGGAAAG13200.078.322835
TTCGGAG6750.075.8810043
GATCCGG7650.075.0293961
TCGGGAA13950.074.453133
TCGGAGA10200.074.393144
ATCGGGA58800.074.377112
GATCGGG60950.073.4692841
AAAGCGG11850.071.619558
AAGGGGC21800.069.379499
AAGCGTC4650.069.335069
ATCCGGA8100.068.5151442
CGGAGAG17850.067.218395
Produced by FastQC (version 0.11.2)