##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2995253 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18069325028637 33.0 33.0 33.0 27.0 33.0 2 30.665338120018575 33.0 33.0 33.0 27.0 33.0 3 30.543203862912414 33.0 33.0 33.0 27.0 33.0 4 33.8824630173144 37.0 37.0 37.0 22.0 37.0 5 34.01164776397854 37.0 37.0 37.0 27.0 37.0 6 34.61322966707654 37.0 37.0 37.0 27.0 37.0 7 33.86986575090652 37.0 37.0 37.0 22.0 37.0 8 34.31012305137496 37.0 37.0 37.0 27.0 37.0 9 33.65703231079311 37.0 37.0 37.0 22.0 37.0 10-11 33.6866404941419 37.0 37.0 37.0 18.5 37.0 12-13 33.65734831081048 37.0 37.0 37.0 18.5 37.0 14-15 35.18081177115923 40.0 37.0 40.0 18.5 40.0 16-17 35.11652387961885 40.0 37.0 40.0 18.5 40.0 18-19 35.185444768772456 40.0 37.0 40.0 18.5 40.0 20-21 35.200523461624115 40.0 37.0 40.0 18.5 40.0 22-23 36.002829977968474 40.0 37.0 40.0 27.0 40.0 24-25 35.222193417384105 40.0 37.0 40.0 22.0 40.0 26-27 35.13700979516588 40.0 37.0 40.0 21.0 40.0 28-29 34.78261736153841 40.0 37.0 40.0 15.0 40.0 30-31 34.74482940172333 40.0 37.0 40.0 15.0 40.0 32-33 34.535791634296004 40.0 37.0 40.0 15.0 40.0 34-35 34.87936711857061 38.5 37.0 40.0 18.5 40.0 36-37 34.52228793360695 37.0 37.0 40.0 15.0 40.0 38-39 34.466451248024796 37.0 37.0 40.0 15.0 40.0 40-41 34.234744610889294 37.0 37.0 40.0 15.0 40.0 42-43 34.149283549670095 37.0 37.0 40.0 15.0 40.0 44-45 33.99143528109312 37.0 35.0 40.0 15.0 40.0 46-47 33.95985364174579 37.0 33.0 40.0 15.0 40.0 48-49 33.85724761814778 37.0 33.0 40.0 15.0 40.0 50-51 32.868198946800156 37.0 33.0 38.5 15.0 38.5 52-53 33.33044654324693 37.0 33.0 38.5 15.0 40.0 54-55 33.935036539484315 37.0 33.0 40.0 15.0 40.0 56-57 34.225050772004906 37.0 33.0 40.0 18.5 40.0 58-59 33.60418986309337 37.0 33.0 40.0 15.0 40.0 60-61 33.45617164893917 37.0 33.0 40.0 15.0 40.0 62-63 33.012586916697856 37.0 33.0 40.0 6.0 40.0 64-65 32.84415106169662 37.0 33.0 38.5 10.5 40.0 66-67 32.72866198614942 37.0 33.0 37.0 15.0 40.0 68-69 32.85703244433776 37.0 33.0 37.0 15.0 40.0 70-71 32.37777326322684 37.0 33.0 37.0 15.0 40.0 72-73 32.69370483895685 37.0 33.0 37.0 18.5 37.0 74-75 32.789722270539414 37.0 33.0 37.0 22.0 37.0 76-77 32.47015427411307 37.0 33.0 37.0 22.0 37.0 78-79 31.97352126848717 37.0 33.0 37.0 22.0 37.0 80-81 31.45010438183352 37.0 33.0 37.0 15.0 37.0 82-83 31.066416426258485 35.0 33.0 37.0 15.0 37.0 84-85 30.849339938896648 33.0 33.0 37.0 15.0 37.0 86-87 30.675128611840137 33.0 33.0 37.0 15.0 37.0 88-89 30.537948213389654 33.0 33.0 37.0 15.0 37.0 90-91 30.402508903254585 33.0 27.0 37.0 15.0 37.0 92-93 30.271331503549114 33.0 27.0 37.0 6.0 37.0 94-95 30.13655641109449 33.0 27.0 37.0 6.0 37.0 96-97 29.982564744948093 33.0 27.0 37.0 6.0 37.0 98-99 29.854578728407915 33.0 27.0 37.0 6.0 37.0 100-101 28.693758590676648 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.2601206535586478 2306 2 -0.39635157481144745 2306 3 -0.5385584919920987 2306 4 -0.4479312465732974 2306 5 -0.5214629883607245 2306 6 -0.5012196702936436 2306 7 -0.05911904049867189 2306 8 -0.4879158536457311 2306 9 -0.4011694095509597 2306 10-11 -0.4603537794913919 2306 12-13 -0.39250615950719947 2306 14-15 -0.021254170289168428 2306 16-17 -0.20082992720745096 2306 18-19 -0.19170087036501826 2306 20-21 -0.23499586791112392 2306 22-23 -0.11226727936608683 2306 24-25 -0.20786114045352377 2306 26-27 -0.31574560852168077 2306 28-29 -0.4037932951307539 2306 30-31 -0.36619269304758717 2306 32-33 -0.33476252399143647 2306 34-35 -0.5657116935218554 2306 36-37 -0.22611912478015483 2306 38-39 -0.4321633353689691 2306 40-41 -0.3953333714228009 2306 42-43 -0.2839373607288138 2306 44-45 -0.32456092489087496 2306 46-47 -0.4947247129489085 2306 48-49 -0.4977552377356247 2306 50-51 -0.334088214466675 2306 52-53 -0.5272012242807804 2306 54-55 -0.36794452402492084 2306 56-57 -0.31854782763410583 2306 58-59 -0.019828272543350067 2306 60-61 -0.25282361579639456 2306 62-63 -0.19109843233707124 2306 64-65 -0.2394656212386863 2306 66-67 -0.19782699464250442 2306 68-69 -0.2381149160331617 2306 70-71 -0.16164072162352028 2306 72-73 -0.3507852736866184 2306 74-75 -0.25565981935306326 2306 76-77 0.0067649659975757 2306 78-79 -0.08937166634445859 2306 80-81 0.02465208646653494 2306 82-83 -0.09536652037688853 2306 84-85 -0.17701593291885231 2306 86-87 0.05157800659074141 2306 88-89 0.0028865212752826608 2306 90-91 -0.11757223887389046 2306 92-93 -0.19050912226839145 2306 94-95 0.013752048536435524 2306 96-97 -0.08303121352646414 2306 98-99 -0.11127433031211353 2306 100-101 -0.07528427046404218 2307 1 -0.041410717661211294 2307 2 -0.10780990814478031 2307 3 -0.05157932532543441 2307 4 -0.05502259272714127 2307 5 -0.1374886293863682 2307 6 -0.10109947798595442 2307 7 0.431465895398766 2307 8 -0.18619309723547417 2307 9 0.09358219301314108 2307 10-11 0.02165743845732493 2307 12-13 0.11438486613382537 2307 14-15 0.34695496432621553 2307 16-17 0.39101702792076054 2307 18-19 0.3618448027119072 2307 20-21 0.2333935551658044 2307 22-23 0.10417903473647527 2307 24-25 0.3561011992900731 2307 26-27 0.10941464788857758 2307 28-29 0.11195189717693665 2307 30-31 0.2990917620806144 2307 32-33 0.23729276447010506 2307 34-35 -0.010443264034670108 2307 36-37 0.27784722137369045 2307 38-39 0.044419196682312645 2307 40-41 0.21041582729514374 2307 42-43 0.2926852354250329 2307 44-45 0.28144869049373966 2307 46-47 0.18826807551263158 2307 48-49 0.145534025084892 2307 50-51 -0.049172349082056144 2307 52-53 -0.14148407684488262 2307 54-55 0.034639610590467385 2307 56-57 0.04677669159666209 2307 58-59 0.267110830020755 2307 60-61 0.16552574317797308 2307 62-63 0.11402977279112747 2307 64-65 0.230526365940797 2307 66-67 0.11264976817800942 2307 68-69 0.060803353197606214 2307 70-71 0.07644020786366212 2307 72-73 -0.11582934419944735 2307 74-75 0.009003642185398064 2307 76-77 0.13158467753603276 2307 78-79 0.11509548109143708 2307 80-81 0.36167131723576773 2307 82-83 0.196540530905164 2307 84-85 0.22671002861961043 2307 86-87 0.11564050659074354 2307 88-89 0.21955318794195122 2307 90-91 0.33074506881841614 2307 92-93 0.1999155572187874 2307 94-95 0.12651246520310266 2307 96-97 0.20743353006328036 2307 98-99 0.30266797738019235 2307 100-101 0.003161242356469529 2201 1 0.06742002552336857 2201 2 -0.2044380119822513 2201 3 -0.10112785805680957 2201 4 0.007567483675011033 2201 5 -0.08679406435771853 2201 6 -0.0923490438828054 2201 7 0.44201785754497536 2201 8 0.029429189619882834 2201 9 0.10368268789072488 2201 10-11 0.06859538341304727 2201 12-13 0.14850211739272368 2201 14-15 0.3235486654896107 2201 16-17 0.32319600864657616 2201 18-19 0.29566686383362395 2201 20-21 0.2033612144816601 2201 22-23 0.2529738558859833 2201 24-25 0.2952421091702604 2201 26-27 0.08481402278005135 2201 28-29 0.20490803105434452 2201 30-31 0.2786465893075629 2201 32-33 0.2510603456473959 2201 34-35 0.0785891200597888 2201 36-37 0.16621651291736583 2201 38-39 0.07234722238122515 2201 40-41 0.2954262457705781 2201 42-43 0.17553816128022248 2201 44-45 0.2591227658540447 2201 46-47 0.3236849230556018 2201 48-49 0.2391657319784457 2201 50-51 0.19966425449495517 2201 52-53 0.09209488651681141 2201 54-55 0.33310125757778764 2201 56-57 0.40695478241103444 2201 58-59 0.5279872842662812 2201 60-61 0.34095224951587255 2201 62-63 0.37950446457789866 2201 64-65 0.2800450540810999 2201 66-67 0.3737617233785713 2201 68-69 0.34121282098715255 2201 70-71 0.38445537976850375 2201 72-73 0.15678091799885152 2201 74-75 0.34923577884107004 2201 76-77 0.36393729951902287 2201 78-79 0.28308471533349433 2201 80-81 0.42365744764035185 2201 82-83 0.47811448037555593 2201 84-85 0.40766592374029287 2201 86-87 0.39728554045078823 2201 88-89 0.5214719238335981 2201 90-91 0.49207787399292613 2201 92-93 0.4949510451507244 2201 94-95 0.49912109951461403 2201 96-97 0.517417902350136 2201 98-99 0.27124542138088614 2201 100-101 0.2161002291597356 1101 1 -0.021832556701589567 1101 2 -0.27479200243308455 1101 3 -0.20375520927828816 1101 4 -0.07681412281989708 1101 5 -0.0958434658463716 1101 6 -0.11435571970434211 1101 7 0.1787072476548488 1101 8 0.0034849660883295996 1101 9 0.011086464580195354 1101 10-11 0.021779701167417898 1101 12-13 0.0028851973982142454 1101 14-15 0.34081022794292437 1101 16-17 0.1790004123966611 1101 18-19 0.054888686525281116 1101 20-21 0.10768177261471834 1101 22-23 0.1127202074123943 1101 24-25 0.09065120277706029 1101 26-27 0.06646011828400589 1101 28-29 0.07624835264802243 1101 30-31 0.16086254456195803 1101 32-33 0.13934573190158517 1101 34-35 0.05286571846061605 1101 36-37 0.007911643578871974 1101 38-39 0.0064937834735872 1101 40-41 -0.013423003864623695 1101 42-43 -0.013305419432349197 1101 44-45 0.008380484900598617 1101 46-47 0.022712815382909923 1101 48-49 -0.03849380112335865 1101 50-51 -0.033550618138470156 1101 52-53 -0.016241294921456984 1101 54-55 0.08306196396238619 1101 56-57 0.06572871928338486 1101 58-59 -0.12978313050648183 1101 60-61 0.057244569455953354 1101 62-63 0.21756166134682076 1101 64-65 0.14071296368123143 1101 66-67 0.044142114227248896 1101 68-69 0.09372863564408362 1101 70-71 0.2637944079473513 1101 72-73 -0.14424746959173262 1101 74-75 0.057661803493715524 1101 76-77 0.1670713746557766 1101 78-79 0.16302071995049516 1101 80-81 0.1869112659769634 1101 82-83 0.24278133395976909 1101 84-85 0.2507336704356362 1101 86-87 0.254302111305158 1101 88-89 0.24851964306434482 1101 90-91 0.011507543537714326 1101 92-93 0.1659188960150786 1101 94-95 0.1829522884125332 1101 96-97 0.30267727090992125 1101 98-99 0.2222535482010315 1101 100-101 0.11264259565865231 2304 1 -0.02968406154896286 2304 2 -0.15884400822548983 2304 3 -0.12950507189524885 2304 4 -0.05277007829242564 2304 5 -0.06494563565693312 2304 6 0.04641829379029616 2304 7 0.255712729073565 2304 8 -0.01490460465461041 2304 9 0.02832135921417489 2304 10-11 0.009270161186577752 2304 12-13 0.028680281170771593 2304 14-15 0.0445253141741091 2304 16-17 0.16592816903952468 2304 18-19 0.08943878358977742 2304 20-21 0.12391126485724158 2304 22-23 0.06815102672754136 2304 24-25 0.10188020841653866 2304 26-27 0.18559115499729018 2304 28-29 0.12594717097093877 2304 30-31 0.1900541542082621 2304 32-33 0.05882920825230542 2304 34-35 0.02801749836677203 2304 36-37 0.17101440024002557 2304 38-39 0.10856444409027688 2304 40-41 0.13346946856912467 2304 42-43 0.15791153454963336 2304 44-45 0.19267828011315657 2304 46-47 0.07114683164351021 2304 48-49 0.14076435972200585 2304 50-51 -0.010198610955775678 2304 52-53 -0.05023454248094339 2304 54-55 0.18070673202834797 2304 56-57 0.316037145126181 2304 58-59 0.3335842577229897 2304 60-61 0.2474159927588957 2304 62-63 0.30593042157737216 2304 64-65 0.17886191104540927 2304 66-67 0.101478771096005 2304 68-69 0.09871796330501326 2304 70-71 0.16009618818277005 2304 72-73 0.13064191053452845 2304 74-75 0.1334392524790573 2304 76-77 0.2759531974342124 2304 78-79 0.1469977596038845 2304 80-81 0.2162960533753342 2304 82-83 0.23856709544232046 2304 84-85 0.35453799161706456 2304 86-87 0.42770164460527127 2304 88-89 0.40103018552064285 2304 90-91 0.43135835031093706 2304 92-93 0.37461269976551037 2304 94-95 0.3177781028140778 2304 96-97 0.3947961674259197 2304 98-99 0.28790343401395546 2304 100-101 0.3507447852260306 2202 1 0.15263738848795683 2202 2 -0.019796990211649046 2202 3 -0.004618839003246222 2202 4 -0.03571145174046819 2202 5 -0.024249209232053204 2202 6 0.04506167469116207 2202 7 0.2614182568670884 2202 8 0.1767672137301588 2202 9 0.13827619581884676 2202 10-11 0.11662981979938536 2202 12-13 0.11856273613616253 2202 14-15 0.157010932294412 2202 16-17 0.1928480426507022 2202 18-19 0.0681181835863498 2202 20-21 0.10718213360863871 2202 22-23 0.15666398329855014 2202 24-25 0.14016713335600883 2202 26-27 0.13812343909737024 2202 28-29 0.06292877933732655 2202 30-31 0.11110514763123547 2202 32-33 -0.05200464025283935 2202 34-35 -0.23471261170827518 2202 36-37 -0.054001913533952006 2202 38-39 -0.08939247037403675 2202 40-41 0.02797194139380821 2202 42-43 0.026014671945965517 2202 44-45 0.05504583802705554 2202 46-47 0.0535204428261693 2202 48-49 -0.04036146240634508 2202 50-51 -0.015810763858766563 2202 52-53 0.06272009664120759 2202 54-55 0.2240648794705251 2202 56-57 0.2257887035209052 2202 58-59 0.1395973860179467 2202 60-61 0.12323704783076295 2202 62-63 0.32798084831135554 2202 64-65 0.19354001452625624 2202 66-67 0.34375656032204205 2202 68-69 0.19399469642884526 2202 70-71 0.24701318541801953 2202 72-73 0.27855632332452274 2202 74-75 0.2132801502517978 2202 76-77 0.2594269150857187 2202 78-79 0.17463780047417998 2202 80-81 0.1752314937613697 2202 82-83 0.2479528818520862 2202 84-85 0.36824907467993384 2202 86-87 0.187885947320229 2202 88-89 0.3535957411334394 2202 90-91 0.35963140854252273 2202 92-93 0.2868639147123595 2202 94-95 0.33284795152529156 2202 96-97 0.3015210914380759 2202 98-99 0.22179937789457327 2202 100-101 0.3424462259898604 1102 1 0.09151351701732224 1102 2 -0.03469126076401352 1102 3 0.1117904948465771 1102 4 0.1055614723477376 1102 5 0.070242771347381 1102 6 0.2404425169620268 1102 7 0.2823045163165503 1102 8 0.3720972588119196 1102 9 0.08195013371200588 1102 10-11 0.1861551304643001 1102 12-13 0.2182704889160476 1102 14-15 0.19549292216586878 1102 16-17 0.25171010243810343 1102 18-19 0.25302217986953934 1102 20-21 0.30448688228435117 1102 22-23 0.28166576565606505 1102 24-25 0.14507470623660623 1102 26-27 0.26243232997193644 1102 28-29 0.23609015873945793 1102 30-31 0.20223010633995386 1102 32-33 0.22798486166141885 1102 34-35 0.13698185775518823 1102 36-37 0.2853045949227422 1102 38-39 0.3251936899763592 1102 40-41 0.3543158092522063 1102 42-43 0.32643756597173734 1102 44-45 0.1421977733108406 1102 46-47 0.1870744221175542 1102 48-49 0.3452949561007088 1102 50-51 0.24670183146765368 1102 52-53 0.21920974946173288 1102 54-55 0.37491916342621323 1102 56-57 0.5215378105597921 1102 58-59 0.48493049081344 1102 60-61 0.528910975029774 1102 62-63 0.46819430938823103 1102 64-65 0.4793214357068436 1102 66-67 0.45710870544871796 1102 68-69 0.3732208443264966 1102 70-71 0.4510911897004277 1102 72-73 0.5476043780595319 1102 74-75 0.4263915879964273 1102 76-77 0.41239863976613833 1102 78-79 0.2105185880860816 1102 80-81 0.4101656712984365 1102 82-83 0.5259473315907961 1102 84-85 0.4934553825619936 1102 86-87 0.5299436731271001 1102 88-89 0.6320629612023119 1102 90-91 0.6843237736358816 1102 92-93 0.6725337009007539 1102 94-95 0.7743647912390692 1102 96-97 0.5155378182692125 1102 98-99 0.5404701966620848 1102 100-101 0.5965426776725238 2305 1 0.16702766952278125 2305 2 0.08626961733852312 2305 3 0.10826250244258162 2305 4 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12752.0 17 11492.0 18 10985.0 19 11086.0 20 11497.0 21 12112.0 22 12641.0 23 13168.0 24 14604.0 25 16767.0 26 19088.0 27 23080.0 28 28834.0 29 35622.0 30 41209.0 31 52951.0 32 73135.0 33 104340.0 34 159411.0 35 268271.0 36 537493.0 37 966206.0 38 270979.0 39 394.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.289858659536655 22.942756744426553 23.576477639656403 13.190906956380388 2 30.175567228521043 26.34137269435889 31.84775075394096 11.635309323179102 3 36.24817587568 19.22958084264846 29.954060825099415 14.568182456572131 4 27.409896805173783 17.349906034523116 35.359175511459775 19.881021648843323 5 34.358032139527225 15.964553683819075 33.16343456584349 16.513979610810203 6 30.3595578212638 16.505154694336724 38.16828100608504 14.967006478314435 7 35.47916267591485 19.44240797702397 28.686878006853895 16.39155134020729 8 27.38890972329458 32.75058275058275 24.10127392920625 15.759233596916419 9 33.253047566809826 26.637861390401067 23.178921944653627 16.93016909813548 10-11 27.038709076986233 29.757539231472663 26.823718986046114 16.380032705494987 12-13 28.95411338051183 23.751601614444077 26.858378418135665 20.435906586908427 14-15 24.98389331129955 24.357140481577876 29.55463313600864 21.104333071113935 16-17 26.680500275987022 25.657334578658975 30.396791297827512 17.265373847526487 18-19 26.235782493457354 25.8315925278501 30.752128239151578 17.180496739540963 20-21 24.96569780547836 28.744695922387724 29.072553214563886 17.217053057570038 22-23 28.599041970002446 25.72383235394137 28.394050622017648 17.28307505403854 24-25 24.394469557663555 30.141950479582828 28.189022462705402 17.274557500048214 26-27 22.763379617062824 31.58621310967283 28.13183787973755 17.518569393526796 28-29 23.771703134636528 30.152961011449868 27.863925535623306 18.211410318290298 30-31 26.395392137747166 26.86145032514617 28.507842252851674 18.23531528425499 32-33 22.21724266491398 29.553960943938794 30.584712565610594 17.644083825536626 34-35 27.331677336876396 26.963707891601718 28.556009601095738 17.148605170426148 36-37 23.545490036415064 27.3918898467728 30.33039070860046 18.73222940821167 38-39 20.42854250093333 30.13948600827091 31.556253572736775 17.875717918058985 40-41 22.08405468500219 31.051838339292004 28.593813511675304 18.270293464030505 42-43 24.52807332071109 27.306737261866864 30.503510212288255 17.66167920513379 44-45 21.79173957431757 29.498441138404402 31.111012114504984 17.598807172773046 46-47 23.84558726100096 30.108597892970714 28.3114701949214 17.734344651106927 48-49 20.569726217506705 29.508441117374957 30.41881041259589 19.503022252522452 50-51 19.743262581969645 27.376194048629433 31.852756032942324 21.0277873364586 52-53 25.68104257558894 26.84165437646285 27.771812291901334 19.70549075604688 54-55 27.17482234291473 26.88124914899949 27.65384456279969 18.29008394528609 56-57 27.701090491370188 26.848670390470048 27.068048383425232 18.382190734734536 58-59 25.669922393499377 26.712145790012737 27.545676621994286 20.0722551944936 60-61 21.35163052733084 26.915451217464092 27.363509883863742 24.369408371341326 62-63 24.676095631540257 26.94284848292019 27.663785842151356 20.717270043388197 64-65 21.326323510203263 28.816636021716054 30.07798553826265 19.77905492981804 66-67 20.162543567165912 28.51628542378411 28.5872791160808 22.73389189296918 68-69 20.00588442841921 27.118791940576987 34.02850749360357 18.846816137400232 70-71 19.384030792600022 32.28762442350943 30.05461507240708 18.273729711483462 72-73 19.2346803134821 35.70032565655321 26.624683333657 18.440310696307684 74-75 19.923434577835373 35.26074603150685 26.21648274557275 18.599336645085028 76-77 20.235392319975247 34.61572587976218 26.494133356280038 18.654748443982534 78-79 20.02333284276768 34.43573313697313 27.076980466278467 18.463953553980723 80-81 19.259084872064665 34.65002052832473 27.00477677037545 19.086117829235146 82-83 18.86400125413313 33.8183664179057 27.298565752719384 20.01906657524179 84-85 19.04176276634426 32.76900889445747 27.863516378131763 20.325711961066503 86-87 19.27489478176857 32.47326608677944 28.505578730836433 19.746260400615558 88-89 19.731155988048474 32.82430422760358 27.846405573046322 19.598134211301627 90-91 20.160126235986738 32.08177002360896 27.515261581778315 20.242842158625997 92-93 19.706197403235013 32.66972752202833 27.526248043149586 20.09782703158707 94-95 19.4393371811244 32.97652162076046 27.613094232482556 19.97104696563259 96-97 20.065856603766004 33.20697619951246 26.968913734344657 19.758253462376874 98-99 20.651404730224616 32.19265110867674 26.582789312737297 20.57315484836134 100-101 20.575559783658942 32.215479658829786 26.124699875177548 21.08426068233372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1018.0 1 774.5 2 447.0 3 405.5 4 419.0 5 411.5 6 511.0 7 698.0 8 892.5 9 1078.5 10 1276.0 11 1392.0 12 1379.0 13 1335.0 14 1254.5 15 1158.5 16 1064.5 17 997.5 18 979.0 19 1006.5 20 1073.5 21 1191.0 22 1418.0 23 1715.0 24 2092.0 25 2790.5 26 4242.5 27 6538.5 28 8945.5 29 10906.5 30 12996.0 31 15351.0 32 18897.0 33 27614.5 34 44022.5 35 73321.0 36 110858.5 37 150491.5 38 194560.5 39 223584.0 40 228935.0 41 210049.5 42 185516.0 43 180597.5 44 174655.5 45 161899.5 46 157919.0 47 145506.5 48 118402.0 49 96820.0 50 80815.5 51 69198.5 52 59587.0 53 48270.5 54 37766.0 55 28713.5 56 21197.5 57 15186.0 58 11126.5 59 8258.0 60 6137.5 61 4587.5 62 3374.5 63 2460.0 64 1808.5 65 1357.5 66 996.0 67 754.5 68 584.5 69 416.5 70 313.5 71 242.0 72 192.0 73 151.0 74 125.0 75 113.5 76 92.0 77 76.5 78 66.0 79 51.0 80 49.0 81 46.0 82 38.5 83 38.0 84 28.0 85 18.0 86 21.0 87 20.0 88 15.5 89 11.0 90 9.5 91 10.0 92 7.0 93 5.5 94 4.5 95 3.0 96 3.0 97 3.5 98 3.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1819879656242728 2 0.18793070234801534 3 0.15948569286133757 4 0.16001987144324703 5 0.16829963946284338 6 0.16589583584425088 7 0.1322759713453254 8 0.11374665178534168 9 0.1148483951105299 10-11 0.09313069713977416 12-13 0.13790153953605921 14-15 0.1163006931300962 16-17 0.10898912379021071 18-19 0.11160993745770391 20-21 0.10341363484153092 22-23 0.10601775542833944 24-25 0.45924334271595757 26-27 0.3124443911749692 28-29 0.39439072425601446 30-31 0.25875944369307036 32-33 0.28703752237290137 34-35 0.30499927718960634 36-37 0.28635310606482994 38-39 0.33291010809437466 40-41 0.3281025008571897 42-43 0.37068654968378295 44-45 0.3252145978987418 46-47 0.34836790080837915 48-49 0.34422801679858095 50-51 0.35936864097957666 52-53 0.3502542189257468 54-55 0.32706752985474014 56-57 0.34129003459807905 58-59 0.33387830677408553 60-61 0.30543329728740776 62-63 0.3144308677764449 64-65 0.3152655218106784 66-67 0.32639980662735335 68-69 0.3140469269206975 70-71 0.3031797313949773 72-73 0.2894914052335479 74-75 0.2977377870917749 76-77 0.2878554833264502 78-79 0.2972870739132888 80-81 0.3049825841089217 82-83 0.331324265429331 84-85 0.3290039272141619 86-87 0.32292764584494194 88-89 0.3347797331310577 90-91 0.32496420168847173 92-93 0.3094396366517286 94-95 0.301727433375411 96-97 0.2875383147934415 98-99 0.31069161770307885 100-101 0.31423055080822887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2995253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.78437238756802 #Duplication Level Percentage of deduplicated Percentage of total 1 89.33381940204262 42.68760493110954 2 4.665184471746026 4.458458241092238 3 1.7059611105253145 2.4455484295215215 4 0.7722939847015476 1.4761433343063006 5 0.5775313790110814 1.3798487240085602 6 0.4169808596870983 1.1955101206665935 7 0.3513921832482624 1.1753738456890859 8 0.2503806532079494 0.9571425897224946 9 0.21009109624540293 0.9035164060472454 >10 1.389487532426457 12.982943495577068 >50 0.16799765157075175 5.506021692053393 >100 0.13490413753669434 13.877608202211695 >500 0.019426394073856587 6.327748747027178 >1k 0.004269386952386973 3.4484082037211317 >5k 2.098177719269965E-4 0.540635908488756 >10k+ 6.993925271488707E-5 0.6374871287570917 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 19075 0.6368410281201622 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5504 0.18375743217684784 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5430 0.18128685623551666 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5243 0.17504364405945008 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4571 0.15260814361925354 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4115 0.13738405403483445 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3616 0.12072435951153374 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3251 0.10853841061172463 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3094 0.10329678327673823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005141468850878374 0.0 0.0 2 0.005675647432787815 0.0 0.0 3 0.006276598337435936 0.0 0.0 4 0.006643846112498677 0.0 0.0 5 0.007411727823993499 0.0 0.0 6 0.008313154180965681 0.0 0.0 7 0.010116006894910046 0.0 0.0 8 0.012352879706655832 0.0 0.0 9 0.014189118581969536 0.0 0.0 10-11 0.02266920356978192 0.0 0.0 12-13 0.030498258410892168 0.0 0.0 14-15 0.04003000748183876 0.0 0.0 16-17 0.048960805648137234 0.0 0.0 18-19 0.059060119462362615 0.0 0.0 20-21 0.06800761070934574 0.0 0.0 22-23 0.07715541892454494 0.0 0.0 24-25 0.08845663454806656 0.0 0.0 26-27 0.09954084012268746 0.0 0.0 28-29 0.11264490846015346 0.0 0.0 30-31 0.2077453891207187 0.0 0.0 32-33 0.22572383701810833 0.0 0.0 34-35 0.23874443995215094 0.0 0.0 36-37 0.25208221141920234 0.0 0.0 38-39 0.26710598403540536 0.0 0.0 40-41 0.2828475591210492 0.0 0.0 42-43 0.30054222464679947 3.3386161369340086E-5 0.0 44-45 0.3177194046713249 6.677232273868017E-5 0.0 46-47 0.3376008637667669 6.677232273868017E-5 0.0 48-49 0.3574990159428937 6.677232273868017E-5 0.0 50-51 0.3795672686080274 6.677232273868017E-5 0.0 52-53 0.4027205715176648 6.677232273868017E-5 0.0 54-55 0.428561460417534 6.677232273868017E-5 0.0 56-57 0.4577075792929679 6.677232273868017E-5 0.0 58-59 0.48947451183589497 6.677232273868017E-5 0.0 60-61 0.5240959611759006 6.677232273868017E-5 0.0 62-63 0.5614216895868229 6.677232273868017E-5 0.0 64-65 0.60305423281439 6.677232273868017E-5 0.0 66-67 0.6472074312253422 6.677232273868017E-5 0.0 68-69 0.6932970269957162 6.677232273868017E-5 0.0 70-71 0.7454629041353101 6.677232273868017E-5 0.0 72-73 0.7996152578763798 6.677232273868017E-5 0.0 74-75 0.8573065447225994 6.677232273868017E-5 0.0 76-77 0.9198388249673735 6.677232273868017E-5 0.0 78-79 0.9924203397843188 6.677232273868017E-5 0.0 80-81 1.0712450667773306 6.677232273868017E-5 0.0 82-83 1.1561460751395625 6.677232273868017E-5 0.0 84-85 1.2490430691497512 6.677232273868017E-5 0.0 86-87 1.3495521079521495 6.677232273868017E-5 0.0 88-89 1.4580404393218203 6.677232273868017E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3210 0.0 89.36085 9 AGAGCGG 3410 0.0 87.31768 8 CGGGAGA 4120 0.0 86.908615 4 GAGAGCG 3875 0.0 82.10087 7 ATTCGGA 700 0.0 81.992294 2 TCGGGAG 4560 0.0 81.01878 3 AAGCGGC 935 0.0 80.12028 9 GTTCGGA 545 0.0 80.071335 2 CGGAAAG 1320 0.0 78.32283 5 TTCGGAG 675 0.0 75.881004 3 GATCCGG 765 0.0 75.029396 1 TCGGGAA 1395 0.0 74.45313 3 TCGGAGA 1020 0.0 74.39314 4 ATCGGGA 5880 0.0 74.37711 2 GATCGGG 6095 0.0 73.469284 1 AAAGCGG 1185 0.0 71.61955 8 AAGGGGC 2180 0.0 69.37949 9 AAGCGTC 465 0.0 69.33506 9 ATCCGGA 810 0.0 68.515144 2 CGGAGAG 1785 0.0 67.21839 5 >>END_MODULE