##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_40825-1.342000000356f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1808189 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.637328841177553 33.0 33.0 33.0 27.0 33.0 2 31.388680608055907 33.0 33.0 33.0 27.0 33.0 3 31.322699673540765 33.0 33.0 33.0 27.0 33.0 4 34.93322102943885 37.0 37.0 37.0 33.0 37.0 5 34.9915108431696 37.0 37.0 37.0 33.0 37.0 6 35.23994892126874 37.0 37.0 37.0 33.0 37.0 7 34.89866767246123 37.0 37.0 37.0 33.0 37.0 8 34.99644838011955 37.0 37.0 37.0 33.0 37.0 9 34.74469206482287 37.0 37.0 37.0 33.0 37.0 10-11 34.68434162579244 37.0 37.0 37.0 33.0 37.0 12-13 34.65043338942998 37.0 37.0 37.0 33.0 37.0 14-15 36.25012761387222 40.0 37.0 40.0 33.0 40.0 16-17 36.15056086504232 40.0 37.0 40.0 30.0 40.0 18-19 36.104578393077276 40.0 37.0 40.0 27.0 40.0 20-21 36.07949749721959 40.0 37.0 40.0 27.0 40.0 22-23 36.35283756288751 40.0 37.0 40.0 27.0 40.0 24-25 35.85015172639586 40.0 37.0 40.0 27.0 40.0 26-27 35.71194935927605 38.5 37.0 40.0 27.0 40.0 28-29 35.49058782018915 37.0 37.0 40.0 27.0 40.0 30-31 35.47370545888732 37.0 37.0 40.0 27.0 40.0 32-33 35.308633942580116 37.0 37.0 40.0 27.0 40.0 34-35 35.24563720938464 37.0 37.0 40.0 27.0 40.0 36-37 35.06560044331649 37.0 37.0 40.0 24.5 40.0 38-39 34.93236464772211 37.0 37.0 40.0 22.0 40.0 40-41 34.796024088189895 37.0 37.0 40.0 22.0 40.0 42-43 34.54929545528703 37.0 33.0 40.0 22.0 40.0 44-45 34.32321704202381 37.0 33.0 40.0 22.0 40.0 46-47 34.19247683732176 37.0 33.0 40.0 22.0 40.0 48-49 34.01813167760671 37.0 33.0 40.0 22.0 40.0 50-51 32.78028513612239 35.0 33.0 38.5 18.5 38.5 52-53 33.261235136371255 37.0 33.0 37.0 22.0 40.0 54-55 33.90999364557577 37.0 33.0 40.0 22.0 40.0 56-57 33.92523016122762 37.0 33.0 40.0 22.0 40.0 58-59 33.557496202001005 37.0 33.0 38.5 22.0 40.0 60-61 33.31935046612937 37.0 33.0 37.0 22.0 40.0 62-63 33.02218462782375 37.0 33.0 37.0 15.0 40.0 64-65 32.804960377482665 37.0 33.0 37.0 15.0 40.0 66-67 32.61254160931186 37.0 33.0 37.0 15.0 38.5 68-69 32.51546685661731 37.0 33.0 37.0 18.5 37.0 70-71 32.19316067070422 37.0 33.0 37.0 15.0 37.0 72-73 32.12681970745315 37.0 33.0 37.0 18.5 37.0 74-75 32.06665702534414 37.0 33.0 37.0 22.0 37.0 76-77 31.85608998838064 35.0 33.0 37.0 22.0 37.0 78-79 31.633522823111967 33.0 33.0 37.0 18.5 37.0 80-81 31.362755220831453 33.0 33.0 37.0 15.0 37.0 82-83 31.074900356102155 33.0 33.0 37.0 15.0 37.0 84-85 30.928800031412646 33.0 33.0 37.0 15.0 37.0 86-87 30.767248335212745 33.0 33.0 37.0 15.0 37.0 88-89 30.601695951031665 33.0 27.0 37.0 15.0 37.0 90-91 30.458140161233146 33.0 27.0 37.0 15.0 37.0 92-93 30.31248116209091 33.0 27.0 37.0 15.0 37.0 94-95 30.151197137024948 33.0 27.0 37.0 15.0 37.0 96-97 29.947005539797004 33.0 27.0 37.0 15.0 37.0 98-99 29.786402859435604 33.0 27.0 37.0 15.0 37.0 100-101 28.42371123815044 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.03403047215003241 2306 2 -0.12115735422820961 2306 3 -0.2003667928336661 2306 4 -0.1434721467669533 2306 5 -0.22641479876357806 2306 6 -0.14385312969167074 2306 7 0.2095620120540147 2306 8 -0.1572255012157484 2306 9 -0.05874630088518984 2306 10-11 -0.05907805187485593 2306 12-13 -0.12225548554362575 2306 14-15 0.2232856622021302 2306 16-17 -0.014792045609709703 2306 18-19 -0.019482897796919474 2306 20-21 -0.12234062005391877 2306 22-23 0.11879000124083205 2306 24-25 0.11689051975718456 2306 26-27 -0.04509008675920967 2306 28-29 -0.048647188174022915 2306 30-31 -0.10734580658576931 2306 32-33 -0.1440150588239959 2306 34-35 -0.19744743948599108 2306 36-37 -0.07869507795174258 2306 38-39 -0.05979348837799847 2306 40-41 -0.1556315572460818 2306 42-43 0.04866017668621225 2306 44-45 -0.06781571030779077 2306 46-47 -0.06865419766685221 2306 48-49 0.021766083878546283 2306 50-51 0.12512816442232833 2306 52-53 0.00446188102589673 2306 54-55 0.20235158275323784 2306 56-57 0.22336005446855012 2306 58-59 0.361253273618793 2306 60-61 0.15178810264445275 2306 62-63 0.2656025997039535 2306 64-65 0.30683172661456126 2306 66-67 0.17543678091402626 2306 68-69 0.3294909622263873 2306 70-71 0.2764777706005148 2306 72-73 0.16289085195746367 2306 74-75 0.16454544006461447 2306 76-77 0.14392474321853754 2306 78-79 0.13799902106779172 2306 80-81 0.107347697549379 2306 82-83 0.12353248641111492 2306 84-85 0.2056833731990544 2306 86-87 0.34055851318914776 2306 88-89 0.2903390220035824 2306 90-91 0.2172757415901465 2306 92-93 0.3343621842146014 2306 94-95 0.14598315620513702 2306 96-97 0.16911932776910632 2306 98-99 0.10406522852999345 2306 100-101 0.0024094839938761936 2307 1 0.1350952425772185 2307 2 0.14585766654520071 2307 3 0.24969925595721776 2307 4 0.29191296029600977 2307 5 0.13417591179356947 2307 6 0.13091569954024607 2307 7 0.3283166727549869 2307 8 0.13941400022240913 2307 9 0.22522994285615283 2307 10-11 0.36831515149150107 2307 12-13 0.4853260101418968 2307 14-15 0.6441600097039881 2307 16-17 0.5891799369193151 2307 18-19 0.7114617597081363 2307 20-21 0.5820478959788957 2307 22-23 0.3640443423508799 2307 24-25 0.42998629050396175 2307 26-27 0.3274533243412492 2307 28-29 0.5873427447118829 2307 30-31 0.5536425283455202 2307 32-33 0.5060712307253823 2307 34-35 0.33202709977043554 2307 36-37 0.5195335893541042 2307 38-39 0.5128077154144748 2307 40-41 0.5729989956462163 2307 42-43 0.6771463810113403 2307 44-45 0.5895181024119225 2307 46-47 0.5221192123683025 2307 48-49 0.6391678096695372 2307 50-51 0.39311527425507364 2307 52-53 0.3625728323415487 2307 54-55 0.5301464053802789 2307 56-57 0.5718164303074715 2307 58-59 0.6141575561371724 2307 60-61 0.7443139362441045 2307 62-63 0.6244693835539579 2307 64-65 0.5125737102088976 2307 66-67 0.5565806500948014 2307 68-69 0.5404406799210761 2307 70-71 0.5823750221185762 2307 72-73 0.3896432755219692 2307 74-75 0.4468105407357541 2307 76-77 0.5416849434955147 2307 78-79 0.4901171099140669 2307 80-81 0.3276049682428912 2307 82-83 0.591426115898706 2307 84-85 0.550698287442156 2307 86-87 0.31735621213449505 2307 88-89 0.5143703951792524 2307 90-91 0.5603736429430803 2307 92-93 0.5425714097392671 2307 94-95 0.5867957161284352 2307 96-97 0.592914203661298 2307 98-99 0.3799022904240985 2307 100-101 0.3659180596836613 2201 1 0.1684078025005178 2201 2 0.047746448903783545 2201 3 -0.010061050850069364 2201 4 0.03818333421738629 2201 5 0.0812660364340303 2201 6 0.02229153846641907 2201 7 0.18569922309055897 2201 8 -0.015614762960716178 2201 9 0.09084355742950123 2201 10-11 0.15665647459792353 2201 12-13 0.13680491714093534 2201 14-15 0.30904256003956476 2201 16-17 0.21917514305545893 2201 18-19 0.25206818348570437 2201 20-21 0.37523581544198237 2201 22-23 0.16852900198654197 2201 24-25 0.29813585905620954 2201 26-27 0.3898838133153646 2201 28-29 0.3879299930340352 2201 30-31 0.35126716880573383 2201 32-33 0.31236078010218193 2201 34-35 0.21783965969373043 2201 36-37 0.19479485493416604 2201 38-39 0.21418115740872423 2201 40-41 0.1401178834697987 2201 42-43 0.20432758906503068 2201 44-45 0.22826930087789066 2201 46-47 0.4180348403644629 2201 48-49 0.21807480871076024 2201 50-51 0.32427059544395576 2201 52-53 0.229219524575484 2201 54-55 0.4338952069143147 2201 56-57 0.40020569205243817 2201 58-59 0.49760674118032 2201 60-61 0.5774778863879249 2201 62-63 0.6600470441483992 2201 64-65 0.6243186766518463 2201 66-67 0.5692100844188701 2201 68-69 0.4024588966037186 2201 70-71 0.5760303433074512 2201 72-73 0.6183345506897524 2201 74-75 0.4148810105344154 2201 76-77 0.4036935724504538 2201 78-79 0.38005718661588617 2201 80-81 0.4716653709274574 2201 82-83 0.38240646105690246 2201 84-85 0.6179130525428285 2201 86-87 0.48418267426297135 2201 88-89 0.5077141152176026 2201 90-91 0.6024733553112469 2201 92-93 0.4292540559521143 2201 94-95 0.3117549682856726 2201 96-97 0.48369800040147126 2201 98-99 0.26260363270597864 2201 100-101 0.37340128116017013 1101 1 0.14472777767218403 1101 2 -0.022615587881809063 1101 3 0.17773944313035273 1101 4 0.25531143722049876 1101 5 0.24839694124581513 1101 6 0.2733016982557217 1101 7 0.5317527678095786 1101 8 0.2022422413454521 1101 9 0.2135550185511761 1101 10-11 0.1761984435366415 1101 12-13 0.2502083387958933 1101 14-15 0.4559952698196028 1101 16-17 0.4191326848778445 1101 18-19 0.34043528938975953 1101 20-21 0.277266264636566 1101 22-23 0.3595170921444364 1101 24-25 0.3671997963301834 1101 26-27 0.42255333351640445 1101 28-29 0.3512075488223516 1101 30-31 0.5300204070499888 1101 32-33 0.44815582564234546 1101 34-35 0.40906008444792263 1101 36-37 0.3729758776374368 1101 38-39 0.37501273422353876 1101 40-41 0.28091394197156205 1101 42-43 0.27952274581545566 1101 44-45 0.21044915113198925 1101 46-47 0.162688989412068 1101 48-49 0.2607064829423109 1101 50-51 0.3444580579966967 1101 52-53 0.21132157075791014 1101 54-55 0.45855543373149743 1101 56-57 0.40864366216360537 1101 58-59 0.5023846870666091 1101 60-61 0.5718137068652638 1101 62-63 0.6600412250073049 1101 64-65 0.604545450735543 1101 66-67 0.5219299940713142 1101 68-69 0.5943222286870196 1101 70-71 0.6559159433040023 1101 72-73 0.5307640205444457 1101 74-75 0.43422383553344446 1101 76-77 0.38339748593060463 1101 78-79 0.3052971723051101 1101 80-81 0.40942577749144604 1101 82-83 0.6887930761702918 1101 84-85 0.44684151159116325 1101 86-87 0.5238980967110614 1101 88-89 0.6514102612220078 1101 90-91 0.2716480717897589 1101 92-93 0.34178051149172006 1101 94-95 0.6135295907958387 1101 96-97 0.5945416465459665 1101 98-99 0.47280049209397745 1101 100-101 0.1752918554447369 2304 1 0.16712752365444672 2304 2 0.08661564764026153 2304 3 0.16570246623009055 2304 4 0.2850358424076518 2304 5 0.16658807516078866 2304 6 0.21847500041415202 2304 7 0.4091531271869471 2304 8 0.24573556255521112 2304 9 0.3558193846253701 2304 10-11 0.3515789289125806 2304 12-13 0.24220714087133643 2304 14-15 0.31901331806853506 2304 16-17 0.4386386492431953 2304 18-19 0.40001579930149944 2304 20-21 0.4691452742560429 2304 22-23 0.25015054887843036 2304 24-25 0.17141129496267382 2304 26-27 0.2719485981061851 2304 28-29 0.4164782003922838 2304 30-31 0.39833710655133814 2304 32-33 0.3999610113567371 2304 34-35 0.23160307622843135 2304 36-37 0.3556005592132081 2304 38-39 0.37967814523434384 2304 40-41 0.4894953395021133 2304 42-43 0.3631014371911192 2304 44-45 0.3552230248303303 2304 46-47 0.2300307515157911 2304 48-49 0.40868805222348215 2304 50-51 0.2680849550112683 2304 52-53 0.23236282699108557 2304 54-55 0.5521974130492637 2304 56-57 0.49681018811022426 2304 58-59 0.38328320265046045 2304 60-61 0.5285675478010887 2304 62-63 0.7920041615100928 2304 64-65 0.5938471184485934 2304 66-67 0.6093009104836042 2304 68-69 0.7076361498693586 2304 70-71 0.5830157390795137 2304 72-73 0.480685722463285 2304 74-75 0.24947283786434582 2304 76-77 0.3971736183662138 2304 78-79 0.3502860951111444 2304 80-81 0.365351535034403 2304 82-83 0.4356088339297415 2304 84-85 0.43263961078797664 2304 86-87 0.5928689640517426 2304 88-89 0.7950583744908251 2304 90-91 0.4805163217374435 2304 92-93 0.6438180657050232 2304 94-95 0.6136179918552038 2304 96-97 0.4809715427017842 2304 98-99 0.39082925671623414 2304 100-101 0.6638394079982639 2202 1 0.2128658053477821 2202 2 0.26823861892615497 2202 3 0.23649579275375388 2202 4 0.25390430228735283 2202 5 0.27398110659876806 2202 6 0.32833994196448657 2202 7 0.44861970305801435 2202 8 0.2742408400492522 2202 9 0.46927756190377323 2202 10-11 0.4476225107988583 2202 12-13 0.38061821793409933 2202 14-15 0.48862970667368444 2202 16-17 0.500413703153086 2202 18-19 0.46857214931852553 2202 20-21 0.4646236535546464 2202 22-23 0.5275833033898394 2202 24-25 0.615007935525604 2202 26-27 0.5919338438217636 2202 28-29 0.7042366841058296 2202 30-31 0.6305797577827548 2202 32-33 0.4300972047513625 2202 34-35 0.46290372314705763 2202 36-37 0.4632024205229399 2202 38-39 0.5379700530768119 2202 40-41 0.5793409869881998 2202 42-43 0.5522978961628553 2202 44-45 0.6937929941868148 2202 46-47 0.8026819829310696 2202 48-49 0.7665271170288364 2202 50-51 0.568807914947719 2202 52-53 0.6530165552852694 2202 54-55 0.6656442408781089 2202 56-57 0.7566649151559588 2202 58-59 0.8417657812453996 2202 60-61 0.9388161007462728 2202 62-63 1.0112550978396797 2202 64-65 0.8880282556634427 2202 66-67 0.940617446631606 2202 68-69 0.8586008530812492 2202 70-71 0.7087494809930277 2202 72-73 0.8646432755219635 2202 74-75 0.6029469043721178 2202 76-77 0.6653429521535266 2202 78-79 0.8375088848058425 2202 80-81 0.8341201197580368 2202 82-83 0.8659391029116961 2202 84-85 0.800243741987611 2202 86-87 0.8159276407059259 2202 88-89 1.0173032956121517 2202 90-91 0.9684688810383157 2202 92-93 0.9054285525964083 2202 94-95 1.0618281836609036 2202 96-97 0.8874055456526406 2202 98-99 0.831493199515009 2202 100-101 0.7939483627139623 1102 1 -0.09544309005788776 1102 2 -0.3244691034589877 1102 3 -0.27990694429414376 1102 4 -0.4417946224438296 1102 5 -0.3798018846035802 1102 6 -0.06751538550374647 1102 7 -0.22960121580806003 1102 8 -0.2765704991491873 1102 9 -0.304173073482346 1102 10-11 -0.18553486945528874 1102 12-13 -0.19677181139140743 1102 14-15 -0.21212298149835362 1102 16-17 -0.20750418540996662 1102 18-19 -0.10884720552017768 1102 20-21 -0.11438905416773792 1102 22-23 -0.10705012769520295 1102 24-25 -0.2436333156962931 1102 26-27 -0.2254855110169416 1102 28-29 -0.25301649282477 1102 30-31 -0.2873283556092332 1102 32-33 -0.221310419882073 1102 34-35 -0.16331328565059522 1102 36-37 -0.15682690499026108 1102 38-39 -0.19050502023696225 1102 40-41 -0.1995837441945838 1102 42-43 -0.23751637558689254 1102 44-45 -0.3371649725776038 1102 46-47 -0.2436914286036327 1102 48-49 -0.20806721337194034 1102 50-51 -0.10260948485677801 1102 52-53 -0.11852792174308036 1102 54-55 0.10064912846364393 1102 56-57 0.10325338045409893 1102 58-59 0.1424304928778497 1102 60-61 0.20650916037481437 1102 62-63 0.15401169608014698 1102 64-65 0.2586147659273621 1102 66-67 0.0874991670617078 1102 68-69 0.1274180573823287 1102 70-71 0.15134967649645148 1102 72-73 0.15452597346918395 1102 74-75 0.22417857592637347 1102 76-77 0.20080727277913368 1102 78-79 0.22068581540212762 1102 80-81 0.24031757821356692 1102 82-83 0.20189218209057813 1102 84-85 0.16047834609318556 1102 86-87 0.25267250874111724 1102 88-89 0.4375867755730489 1102 90-91 0.4397787539610931 1102 92-93 0.3148127168192829 1102 94-95 0.25822114553773545 1102 96-97 0.28220515464579776 1102 98-99 0.1385606481953623 1102 100-101 0.1745250978068249 2305 1 0.2681058852296978 2305 2 0.2519444450961643 2305 3 0.23384579709516018 2305 4 0.24727931314087215 2305 5 0.1744603980809245 2305 6 0.40316498320786565 2305 7 0.1402077902334966 2305 8 0.37429120038614627 2305 9 0.13961185034694523 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-2.1149237933966916 1213 62-63 -1.5680539232735669 1213 64-65 -1.2762938521711433 1213 66-67 -1.2202352259677127 1213 68-69 -1.1855217549569055 1213 70-71 -1.4984057566036668 1213 72-73 -1.3446657455912785 1213 74-75 -1.6155071176135678 1213 76-77 -1.1820263260410897 1213 78-79 -1.1076845078539606 1213 80-81 -1.284803860931774 1213 82-83 -1.642565521063183 1213 84-85 -1.7100790638054413 1213 86-87 -1.734494624169315 1213 88-89 -1.964168815130595 1213 90-91 -1.6182553672022486 1213 92-93 -1.765780660455416 1213 94-95 -1.9670725317465276 1213 96-97 -1.7146022482169592 1213 98-99 -1.6983078823206235 1213 100-101 -1.5691395218902393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 695.0 3 151.0 4 194.0 5 202.0 6 358.0 7 2097.0 8 11806.0 9 19867.0 10 19411.0 11 15051.0 12 10604.0 13 8974.0 14 8805.0 15 9685.0 16 9217.0 17 8785.0 18 8336.0 19 8585.0 20 8971.0 21 9234.0 22 9726.0 23 10524.0 24 11933.0 25 13680.0 26 16025.0 27 18808.0 28 22060.0 29 26474.0 30 32831.0 31 43742.0 32 59862.0 33 85910.0 34 133621.0 35 231897.0 36 418015.0 37 448036.0 38 63993.0 39 24.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.57198418788384 22.526281964270378 24.43465036330747 12.46708348453831 2 34.34112633167633 22.24423060301363 31.806813573070166 11.607829492239876 3 39.5600761732576 16.234730175400113 27.99959897150593 16.205594679836356 4 32.89980297263718 14.877753854828999 34.68506918114276 17.53737399139106 5 32.097524171649454 14.22407537278129 37.29230994220076 16.386090513368497 6 27.447283129216505 14.642458109842533 42.376182449368706 15.534076311572258 7 37.8884997161962 15.601899408857447 29.204324893054423 17.30527598189193 8 32.55851550336953 28.74955430996109 22.764850613557392 15.92707957311199 9 34.102304310755294 27.025434895746827 20.88014483606285 17.99211595743503 10-11 28.113728702852903 25.584087059526837 27.35646912951544 18.945715108104817 12-13 29.41368343346822 21.795051422387942 26.057314184670915 22.733950959472924 14-15 26.982457636221298 23.54488185314333 26.431698061392737 23.040962449242638 16-17 27.138022812109437 24.956962592957062 27.72688581903943 20.17812877589407 18-19 27.09554511453549 24.90795056334172 27.257290805354025 20.73921351676876 20-21 25.826937252111197 26.509462712569537 27.160682459029236 20.502917576290034 22-23 28.152530170305596 25.56377553774267 25.441541477786533 20.8421528141652 24-25 26.181760000533327 26.99741919032409 25.648737108798723 21.17208370034386 26-27 24.15427099940865 28.095532899970376 26.087040938708988 21.66315516191199 28-29 24.870026616505786 27.458806598939116 25.295563138415822 22.375603646139275 30-31 26.13597834708265 25.891903415746047 26.237611547705086 21.734506689466215 32-33 24.756722220327266 25.323323524852448 27.970615044717746 21.949339210102544 34-35 23.7382924052908 26.38405348493018 26.54772682389958 23.329927285879435 36-37 24.8234156492378 27.691581992236973 26.142470453266675 21.342531905258554 38-39 25.619515773376524 26.789798547786226 24.751250406444516 22.839435272392734 40-41 25.323591196455148 25.901278052060896 24.213854275210096 24.561276476273864 42-43 26.707680795873202 25.572344716299206 25.375952057543383 22.344022430284213 44-45 24.877553890852557 26.550228080585974 25.656631564755163 22.915586463806303 46-47 25.713018808867893 26.738760297372355 25.18566348264847 22.362557411111283 48-49 24.05255943528158 26.52619924970588 25.940725654287554 23.480515660724986 50-51 23.502008977261564 25.695177177973903 25.810487310889997 24.992326533874536 52-53 26.50077045835404 25.429926306275856 24.28748904944614 23.781814185923963 54-55 26.870550988696923 25.314758143291073 25.069043855991506 22.7456470120205 56-57 27.19339719492881 25.08002122060663 24.6754201669014 23.051161417563158 58-59 26.115603241436407 25.053380269498227 25.03229450815797 23.7987219809074 60-61 24.327872362732855 25.21616534575638 24.888762286629117 25.567200004881645 62-63 26.180487401643386 25.040485504275455 25.24614465377751 23.532882440303645 64-65 25.16903034529514 26.269648546702417 25.864597081698786 22.69672402630366 66-67 24.176549149596923 26.140191525876077 25.180176256184584 24.503083068342413 68-69 23.436369628028565 24.493205008144226 28.46864694891107 23.60177841491614 70-71 23.428041591791033 26.787231345670122 26.62899748517719 23.155729577361658 72-73 23.53493211695917 28.56761736572314 25.236473032589853 22.66097748472784 74-75 23.99562458276697 28.53686724452719 25.15437795495284 22.313130217753 76-77 24.03144565660196 28.073773276350007 24.891325960553715 23.003455106494318 78-79 23.939009606400237 28.24509647031285 25.052217137871267 22.763676785415647 80-81 24.206499227114588 28.793913218822958 24.802257552101466 22.197330001960992 82-83 23.749736436173162 28.3930097324411 25.03026822474504 22.826985606640697 84-85 23.48137073566306 28.37303860683692 25.006956549240854 23.138634108259165 86-87 23.232280919791496 28.56044696828889 24.99230178127072 23.2149703306489 88-89 23.328488150295193 28.636280275573366 25.136511806700224 22.898719767431214 90-91 23.74470304452702 28.042078806213617 24.792400958202773 23.42081719105659 92-93 23.41301156188171 27.802327132768166 24.497969465847888 24.286691839502232 94-95 23.537900509947953 28.32038909810826 23.974182532854282 24.167527859089503 96-97 23.48356316413874 28.905658535557265 23.695433093750225 23.91534520655377 98-99 23.663963753802577 27.671261146984445 24.360222938301966 24.304552160911015 100-101 24.41921717168215 27.860238751402566 23.892172132909664 23.828371944005625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2267.0 1 1507.5 2 676.5 3 981.0 4 1178.5 5 989.5 6 1292.5 7 1770.5 8 2025.5 9 2025.0 10 1813.0 11 1599.5 12 1470.5 13 1375.5 14 1255.0 15 1144.0 16 1089.5 17 1048.0 18 1007.0 19 997.0 20 949.0 21 960.0 22 1031.5 23 1091.0 24 1189.5 25 1396.0 26 1647.0 27 1949.5 28 2348.0 29 2919.0 30 3697.5 31 4672.5 32 6023.5 33 7653.5 34 10106.5 35 14760.5 36 20456.0 37 27605.5 38 35341.5 39 39904.0 40 45457.0 41 49489.5 42 55672.0 43 68156.5 44 75043.0 45 80334.5 46 92395.0 47 99616.5 48 97195.5 49 94105.0 50 91393.0 51 89021.5 52 86648.5 53 83785.0 54 78095.5 55 68558.5 56 59532.0 57 53046.0 58 48630.0 59 41606.0 60 31769.5 61 23190.5 62 16541.5 63 12404.5 64 9902.0 65 8126.5 66 7329.0 67 6675.5 68 5621.5 69 4233.5 70 2925.0 71 2148.0 72 1830.0 73 1459.0 74 962.0 75 683.5 76 535.5 77 403.5 78 330.5 79 271.0 80 218.0 81 167.0 82 131.0 83 103.5 84 77.5 85 59.0 86 48.0 87 37.0 88 30.0 89 28.0 90 21.5 91 17.0 92 16.5 93 10.5 94 8.5 95 6.5 96 2.5 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17940602448084797 2 0.18366442888436996 3 0.15656549177104828 4 0.1579480906033606 5 0.1645292610451673 6 0.16215119105359008 7 0.13073854558345394 8 0.1106632105382789 9 0.11110564216461885 10-11 0.08898406084762157 12-13 0.13593711719294832 14-15 0.11320719238973359 16-17 0.10571350671860077 18-19 0.10831279252334794 20-21 0.10007250348276647 22-23 0.1005702390623989 24-25 0.4519715582829007 26-27 0.30654981310028984 28-29 0.38994817466536963 30-31 0.2568592110669847 32-33 0.28523013910603373 34-35 0.30489069450151507 36-37 0.2851195311994488 38-39 0.33044112092264694 40-41 0.3271228837250973 42-43 0.3703152712465345 44-45 0.3243853380371189 46-47 0.3469216990038099 48-49 0.34448832505894017 50-51 0.36091359918681065 52-53 0.35059941189776067 54-55 0.32587854477601624 56-57 0.3410594799548056 58-59 0.33320631858727157 60-61 0.3051672142679775 62-63 0.31479010214087133 64-65 0.3153431416737963 66-67 0.32773122721131476 68-69 0.3144029744678239 70-71 0.30292740415963154 72-73 0.28951619548620194 74-75 0.29806065626989214 76-77 0.2878847288640734 78-79 0.2975629206902597 80-81 0.3057479057775487 82-83 0.33010929720289195 84-85 0.32798009500113096 86-87 0.3220902239754804 88-89 0.3348930891626926 90-91 0.3238876024574865 92-93 0.3088449271619283 94-95 0.30173836916384295 96-97 0.2873593413077947 98-99 0.31122299715350554 100-101 0.3144859303977626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1808189.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.455422059881805 #Duplication Level Percentage of deduplicated Percentage of total 1 84.20768966200346 44.17149901907938 2 7.163772451676347 7.515574149872741 3 2.7132398543720107 4.269724251323282 4 1.3691546948625917 2.8727834953714377 5 0.8200806091049544 2.1508837236862672 6 0.7374263400323624 2.320920594268289 7 0.43739310546718696 1.6060547967354584 8 0.3243885335057299 1.361274994914334 9 0.29405376978638215 1.3882243142199597 >10 1.7170260770766412 17.294045466748926 >50 0.13286446335168572 4.8078812786082175 >100 0.07877791707528937 7.356083652369739 >500 0.0030785471600915477 1.1146481470625869 >1k 8.433408110356205E-4 1.1078007590708907 >5k 2.1063371431946412E-4 0.6626013566685413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6101 0.33740941903750105 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.32590619675266247 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4503 0.2490337016760969 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 4497 0.24870187795634197 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3800 0.21015502251147417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002986413477794633 0.0 0.0 2 0.003484149057427072 0.0 0.0 3 0.003705364870597045 0.0 0.0 4 0.0038159727771820313 0.0 0.0 5 0.004756139983154416 0.0 0.0 6 0.0053644834693718415 0.0 0.0 7 0.005972826955589266 0.0 0.0 8 0.007134209974731623 0.0 0.0 9 0.008184985087288994 0.0 0.0 10-11 0.015816930641653057 0.0 0.0 12-13 0.028702751758803973 0.0 0.0 14-15 0.03973589044065637 0.0 0.0 16-17 0.052234583884759836 0.0 0.0 18-19 0.062493467220517324 0.0 0.0 20-21 0.06857690208269157 0.0 0.0 22-23 0.07300121834609104 0.0 0.0 24-25 0.07961004076454398 0.0 0.0 26-27 0.08787798178177171 0.0 0.0 28-29 0.09750086965466552 0.0 0.0 30-31 0.1521964794609413 0.0 0.0 32-33 0.16275953453980752 0.0 0.0 34-35 0.16997670044447788 0.0 0.0 36-37 0.17683439065274703 0.0 0.0 38-39 0.18424512039394111 0.0 0.0 40-41 0.1922918455979989 0.0 0.0 42-43 0.19984083522242418 0.0 0.0 44-45 0.20805347228635945 0.0 0.0 46-47 0.21601724156047847 0.0 0.0 48-49 0.225944301176481 0.0 0.0 50-51 0.23675622404516344 0.0 0.0 52-53 0.24955908923237557 0.0 0.0 54-55 0.2634680334854377 0.0 0.0 56-57 0.28044634714623307 0.0 0.0 58-59 0.2976182246435522 0.0 0.0 60-61 0.3167533924827548 0.0 0.0 62-63 0.3372988111309161 0.0 0.0 64-65 0.3595863043077908 0.0 0.0 66-67 0.38173553760143436 0.0 0.0 68-69 0.40819847925189234 0.0 0.0 70-71 0.4361546276412477 0.0 0.0 72-73 0.46753962113473757 0.0 0.0 74-75 0.505367525186803 0.0 0.0 76-77 0.5491129522411651 0.0 0.0 78-79 0.6023153553085435 0.0 0.0 80-81 0.6564302736052481 0.0 0.0 82-83 0.7181218335030243 0.0 0.0 84-85 0.7862010000060834 0.0 0.0 86-87 0.8595340420719295 0.0 0.0 88-89 0.9415498048046969 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1335 0.0 68.90452 9 CGGGAGA 1885 0.0 60.630768 4 AGAGCGG 1635 0.0 57.711525 8 ATCGGGA 2320 0.0 56.221745 2 GGAGAGC 1795 0.0 54.68664 6 TCGGGAG 2090 0.0 54.45683 3 GATCGGG 2815 0.0 48.86831 1 ATTGGGA 1000 0.0 48.853626 2 GATTGGG 1120 0.0 45.318287 1 AGAGCGT 3950 0.0 43.214813 8 GAGCGTC 4130 0.0 42.020214 9 TTGGGAG 1160 0.0 40.88174 3 TGTGTTT 40725 0.0 40.46471 1 TGGGAGA 1280 0.0 39.642513 4 CGGAAAG 675 0.0 39.343376 5 CGGAAGA 4630 0.0 39.02396 4 AAGCGGC 390 0.0 38.905647 9 AAGAGCG 4455 0.0 38.635464 7 ATCGGAA 4950 0.0 37.944565 2 GATCGGA 5020 0.0 37.608593 1 >>END_MODULE