FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808189
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61010.33740941903750105No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.32590619675266247No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA45030.2490337016760969No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT44970.24870187795634197Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38000.21015502251147417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC13350.068.904529
CGGGAGA18850.060.6307684
AGAGCGG16350.057.7115258
ATCGGGA23200.056.2217452
GGAGAGC17950.054.686646
TCGGGAG20900.054.456833
GATCGGG28150.048.868311
ATTGGGA10000.048.8536262
GATTGGG11200.045.3182871
AGAGCGT39500.043.2148138
GAGCGTC41300.042.0202149
TTGGGAG11600.040.881743
TGTGTTT407250.040.464711
TGGGAGA12800.039.6425134
CGGAAAG6750.039.3433765
CGGAAGA46300.039.023964
AAGCGGC3900.038.9056479
AAGAGCG44550.038.6354647
ATCGGAA49500.037.9445652
GATCGGA50200.037.6085931
Produced by FastQC (version 0.11.2)