##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7531203 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.879384475494817 33.0 33.0 33.0 27.0 33.0 2 31.723286173536952 33.0 33.0 33.0 27.0 33.0 3 31.676879244922755 33.0 33.0 33.0 27.0 33.0 4 35.33041069268748 37.0 37.0 37.0 33.0 37.0 5 35.374717691184266 37.0 37.0 37.0 33.0 37.0 6 35.52268050668665 37.0 37.0 37.0 33.0 37.0 7 35.258512750220646 37.0 37.0 37.0 33.0 37.0 8 35.35274975325987 37.0 37.0 37.0 33.0 37.0 9 35.145260989512565 37.0 37.0 37.0 33.0 37.0 10-11 35.09679748374862 37.0 37.0 37.0 33.0 37.0 12-13 35.069173610112486 37.0 37.0 37.0 33.0 37.0 14-15 36.756704672547 40.0 37.0 40.0 33.0 40.0 16-17 36.70838430460579 40.0 37.0 40.0 33.0 40.0 18-19 36.72624466502894 40.0 37.0 40.0 33.0 40.0 20-21 36.728305623948785 40.0 37.0 40.0 33.0 40.0 22-23 36.903579082916764 40.0 37.0 40.0 33.0 40.0 24-25 36.53513044330368 40.0 37.0 40.0 33.0 40.0 26-27 36.46411894620289 40.0 37.0 40.0 33.0 40.0 28-29 36.29667995936373 40.0 37.0 40.0 33.0 40.0 30-31 36.30559115190495 40.0 37.0 40.0 33.0 40.0 32-33 36.213369763104254 40.0 37.0 40.0 33.0 40.0 34-35 36.210354242210705 40.0 37.0 40.0 33.0 40.0 36-37 36.08949048379124 40.0 37.0 40.0 30.0 40.0 38-39 35.96882974738564 40.0 37.0 40.0 27.0 40.0 40-41 35.85319450292337 37.0 37.0 40.0 27.0 40.0 42-43 35.76436626392888 37.0 37.0 40.0 27.0 40.0 44-45 35.61000739191335 37.0 37.0 40.0 27.0 40.0 46-47 35.52873870217016 37.0 37.0 40.0 27.0 40.0 48-49 35.416327909365876 37.0 37.0 40.0 27.0 40.0 50-51 34.25027887310965 37.0 35.0 38.5 24.5 38.5 52-53 34.6977326862654 37.0 35.0 38.5 27.0 40.0 54-55 35.42322547938224 37.0 37.0 40.0 27.0 40.0 56-57 35.48894386461233 37.0 37.0 40.0 27.0 40.0 58-59 35.196055397789706 37.0 37.0 40.0 27.0 40.0 60-61 35.0174982004867 37.0 37.0 40.0 27.0 40.0 62-63 34.69570877321989 37.0 33.0 40.0 27.0 40.0 64-65 34.50978575401566 37.0 33.0 40.0 27.0 40.0 66-67 34.28594508739175 37.0 33.0 37.0 27.0 40.0 68-69 34.1589674982868 37.0 33.0 37.0 27.0 40.0 70-71 33.79297377855835 37.0 33.0 37.0 27.0 40.0 72-73 33.692443624212494 37.0 33.0 37.0 27.0 38.5 74-75 33.53095786954621 37.0 33.0 37.0 27.0 37.0 76-77 33.25375248814831 37.0 33.0 37.0 27.0 37.0 78-79 32.9719664972515 37.0 33.0 37.0 22.0 37.0 80-81 32.68361814174973 37.0 33.0 37.0 22.0 37.0 82-83 32.41948231909298 37.0 33.0 37.0 22.0 37.0 84-85 32.24581570832707 37.0 33.0 37.0 22.0 37.0 86-87 32.0841416703281 35.0 33.0 37.0 22.0 37.0 88-89 31.941433659403415 33.0 33.0 37.0 22.0 37.0 90-91 31.803284349127225 33.0 33.0 37.0 22.0 37.0 92-93 31.66830763956303 33.0 33.0 37.0 15.0 37.0 94-95 31.523936082987007 33.0 33.0 37.0 15.0 37.0 96-97 31.36293458030543 33.0 33.0 37.0 15.0 37.0 98-99 31.21587421026893 33.0 33.0 37.0 15.0 37.0 100-101 29.971034242470957 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.24423943279213134 2306 2 0.2497682170022344 2306 3 0.2402152663622772 2306 4 0.31973870234937607 2306 5 0.3401193303265586 2306 6 0.2600112141123745 2306 7 0.44323282887854987 2306 8 0.21645598572273883 2306 9 0.3490978938014848 2306 10-11 0.3811987097673466 2306 12-13 0.3669260814273301 2306 14-15 0.7200113876287872 2306 16-17 0.6768630759522125 2306 18-19 0.698809836454501 2306 20-21 0.5764803732112895 2306 22-23 0.5247075619142976 2306 24-25 0.5112826595571818 2306 26-27 0.587147537824606 2306 28-29 0.5258134564109298 2306 30-31 0.4835482282697612 2306 32-33 0.5364243147232131 2306 34-35 0.5379918099600687 2306 36-37 0.5537944862306219 2306 38-39 0.5163837639185473 2306 40-41 0.492641868845638 2306 42-43 0.42261512301637083 2306 44-45 0.558701135179021 2306 46-47 0.45969982629197403 2306 48-49 0.5165161316898335 2306 50-51 0.49413886977937693 2306 52-53 0.4435505123481178 2306 54-55 0.577145711639389 2306 56-57 0.6132287937516381 2306 58-59 0.752250604213323 2306 60-61 0.601503599129515 2306 62-63 0.697668476735771 2306 64-65 0.6158179672881445 2306 66-67 0.6907402381141239 2306 68-69 0.6122225412575801 2306 70-71 0.6264858693383957 2306 72-73 0.5926984185652486 2306 74-75 0.5369093922172326 2306 76-77 0.6032442577706547 2306 78-79 0.6164637865305593 2306 80-81 0.7497841616707817 2306 82-83 0.7049703020101674 2306 84-85 0.5556817032095793 2306 86-87 0.6795907595371133 2306 88-89 0.6343041579661275 2306 90-91 0.6766813420415758 2306 92-93 0.5058115339083962 2306 94-95 0.62821603190838 2306 96-97 0.6769293004540202 2306 98-99 0.5536453773970393 2306 100-101 0.4001041709215549 2307 1 0.12956184532376014 2307 2 0.11208283968376165 2307 3 0.13521574796535774 2307 4 0.21639282075739885 2307 5 0.19029149545454516 2307 6 0.20959885343440732 2307 7 0.3306412844253117 2307 8 0.15574321954134973 2307 9 0.26704022390563154 2307 10-11 0.25025186854136194 2307 12-13 0.24003598867248144 2307 14-15 0.3740888514112868 2307 16-17 0.41398864550367875 2307 18-19 0.4288737014882642 2307 20-21 0.38789501209509325 2307 22-23 0.32872588579524376 2307 24-25 0.29178053348688593 2307 26-27 0.26833191940478685 2307 28-29 0.2652031983290897 2307 30-31 0.375387790974159 2307 32-33 0.34196909711197776 2307 34-35 0.24568210218151165 2307 36-37 0.19889966068894438 2307 38-39 0.1902412802117226 2307 40-41 0.36145203334977083 2307 42-43 0.29268204032926803 2307 44-45 0.21718841671142997 2307 46-47 0.14388693132163866 2307 48-49 0.17975067207144946 2307 50-51 0.1147661689556756 2307 52-53 0.16605970745859366 2307 54-55 0.20808150948347048 2307 56-57 0.14871955207555487 2307 58-59 0.2957625256437595 2307 60-61 0.20395181723000633 2307 62-63 0.15767277607983488 2307 64-65 0.14144347719736317 2307 66-67 0.22799191750552694 2307 68-69 0.1661445046544685 2307 70-71 0.19322453460257094 2307 72-73 0.10712362688250465 2307 74-75 0.14492941510454216 2307 76-77 0.16920338784876776 2307 78-79 0.1964613274843643 2307 80-81 0.28735430508643134 2307 82-83 0.21649576541758364 2307 84-85 0.17155089949377356 2307 86-87 0.14005585096834494 2307 88-89 0.2763713259677729 2307 90-91 0.2507212774621195 2307 92-93 0.16481334932875313 2307 94-95 0.23324557076227137 2307 96-97 0.2517630038668983 2307 98-99 0.25401948890314685 2307 100-101 0.029083695454925618 2201 1 0.23402037937762188 2201 2 0.2099240833801055 2201 3 0.18448271848623676 2201 4 0.23221260663043353 2201 5 0.21140409825918027 2201 6 0.2730833241527151 2201 7 0.33330658976494476 2201 8 0.2519370506991976 2201 9 0.2766121956110865 2201 10-11 0.3491487987865085 2201 12-13 0.30807488802829397 2201 14-15 0.4973820688437911 2201 16-17 0.46593544657318375 2201 18-19 0.4769269004187606 2201 20-21 0.40811015441394005 2201 22-23 0.36534661707604954 2201 24-25 0.32079456794124894 2201 26-27 0.4068476968842276 2201 28-29 0.3803951567221233 2201 30-31 0.32626579050032944 2201 32-33 0.35109135142440806 2201 34-35 0.2678259948247117 2201 36-37 0.24948560479932524 2201 38-39 0.34812621235220575 2201 40-41 0.34815145459775465 2201 42-43 0.28460122939954147 2201 44-45 0.23441686581396226 2201 46-47 0.2436201187447793 2201 48-49 0.3193074698692655 2201 50-51 0.2531914106664388 2201 52-53 0.2935298391162249 2201 54-55 0.5136618691445705 2201 56-57 0.5469696169454252 2201 58-59 0.5531619209438006 2201 60-61 0.528652146656114 2201 62-63 0.6177015162506478 2201 64-65 0.43299572846374446 2201 66-67 0.4915749622149619 2201 68-69 0.4358447776719885 2201 70-71 0.44635013394371725 2201 72-73 0.3886083570802725 2201 74-75 0.47083815730605494 2201 76-77 0.4812172869225435 2201 78-79 0.350612011720429 2201 80-81 0.3999100085578178 2201 82-83 0.4119232860349129 2201 84-85 0.5195196265777824 2201 86-87 0.5187982781166625 2201 88-89 0.402489485017405 2201 90-91 0.4310328502461047 2201 92-93 0.44075889812781455 2201 94-95 0.39277213356193386 2201 96-97 0.4204341023675582 2201 98-99 0.4153947018455284 2201 100-101 0.24713035099030023 1101 1 0.03956320444940431 1101 2 -0.04843534830629537 1101 3 0.03551140703624256 1101 4 0.03290051787329418 1101 5 0.039596831237368235 1101 6 0.1418583243077549 1101 7 0.16314786009840532 1101 8 0.09601778918439408 1101 9 0.22046114971313102 1101 10-11 0.12680757496496398 1101 12-13 0.07678748469556496 1101 14-15 0.24062962386739173 1101 16-17 0.25601143154052863 1101 18-19 0.14632415249474207 1101 20-21 0.1483974585212522 1101 22-23 0.13428208334251224 1101 24-25 -0.00983887784755666 1101 26-27 0.036799433019027106 1101 28-29 0.06551041325084839 1101 30-31 0.06904210229141228 1101 32-33 0.09518891171935451 1101 34-35 -0.016453675126733458 1101 36-37 -0.09176232542039742 1101 38-39 0.007812706532014602 1101 40-41 -0.03970389644317862 1101 42-43 -0.0638362802904453 1101 44-45 -0.1264637442983414 1101 46-47 -0.06851561543032147 1101 48-49 -0.14485424586230522 1101 50-51 -0.03417440245488734 1101 52-53 -0.02240212015174592 1101 54-55 0.1368153460653545 1101 56-57 0.07622561233144864 1101 58-59 -0.05000670335774515 1101 60-61 -0.0190542732286616 1101 62-63 0.13103299427531567 1101 64-65 0.06176040297335561 1101 66-67 -0.07845624728090428 1101 68-69 0.01967677042853211 1101 70-71 0.022988516197258946 1101 72-73 -0.0729588464372739 1101 74-75 0.003078899884307873 1101 76-77 -0.010157698348685074 1101 78-79 -0.13480502962556784 1101 80-81 0.027780413955682093 1101 82-83 0.10295020713210334 1101 84-85 0.018427914381121013 1101 86-87 -0.11999002444645157 1101 88-89 -0.06711629548355802 1101 90-91 -0.2264286688950996 1101 92-93 0.04655901161688902 1101 94-95 0.07030664540387477 1101 96-97 0.07778740378338256 1101 98-99 -0.03176683093916921 1101 100-101 -0.18397999698084888 2304 1 0.17327258141839863 2304 2 0.060716016460698796 2304 3 0.07202232692849364 2304 4 0.17683387447780774 2304 5 0.11568558225548031 2304 6 0.15903552996572046 2304 7 0.21612572064255886 2304 8 0.17463881799016434 2304 9 0.1476829438334235 2304 10-11 0.2068525229231355 2304 12-13 0.19166862329301892 2304 14-15 0.28275983436181207 2304 16-17 0.2980767544062246 2304 18-19 0.274679003438429 2304 20-21 0.26195547401634656 2304 22-23 0.33823970728899866 2304 24-25 0.15639194812571588 2304 26-27 0.22246815841688772 2304 28-29 0.16363324118240996 2304 30-31 0.21124157748235461 2304 32-33 0.19067550993361948 2304 34-35 0.10461622568534068 2304 36-37 0.13567416456092474 2304 38-39 0.13603337770801716 2304 40-41 0.2335294558803085 2304 42-43 0.16157092422592712 2304 44-45 0.19364280368530018 2304 46-47 0.18010220535523302 2304 48-49 0.1734307558610766 2304 50-51 -0.00633052958139757 2304 52-53 0.037196967881378384 2304 54-55 0.36594312680691843 2304 56-57 0.3864525145858906 2304 58-59 0.24874805515983667 2304 60-61 0.383457381571894 2304 62-63 0.38292565759619634 2304 64-65 0.34307760948752986 2304 66-67 0.3034362760835805 2304 68-69 0.29579015102871153 2304 70-71 0.29352072373228566 2304 72-73 0.26451734725360154 2304 74-75 0.34129249914577287 2304 76-77 0.36675112856421777 2304 78-79 0.32031897997708825 2304 80-81 0.27168156686710176 2304 82-83 0.3185575822306177 2304 84-85 0.3325069518762618 2304 86-87 0.39855824624130065 2304 88-89 0.4152121646228295 2304 90-91 0.39075401152790334 2304 92-93 0.33429164110674847 2304 94-95 0.38476156188008304 2304 96-97 0.3512880179449951 2304 98-99 0.2643094776806052 2304 100-101 0.2611966526934637 2202 1 0.15407198227594066 2202 2 0.08001245000131618 2202 3 0.03489714590796211 2202 4 0.06794115221298824 2202 5 0.05411730557092653 2202 6 0.1663011971151107 2202 7 0.12328880949169019 2202 8 0.16966607755230712 2202 9 0.1376629250924779 2202 10-11 0.2221017278525821 2202 12-13 0.17957130523639364 2202 14-15 0.24682459711843308 2202 16-17 0.25638701649555173 2202 18-19 0.1827054892265494 2202 20-21 0.22508407667983477 2202 22-23 0.1779291683447397 2202 24-25 0.1220672611847462 2202 26-27 0.20562881236241992 2202 28-29 0.1646767712957029 2202 30-31 0.14543617810137022 2202 32-33 0.012737653216696287 2202 34-35 0.02191735156204544 2202 36-37 0.04423128896106476 2202 38-39 0.06136950798601504 2202 40-41 0.16961589850745185 2202 42-43 0.07763862590408621 2202 44-45 0.1415017922419608 2202 46-47 0.08547372337211101 2202 48-49 0.153096116340393 2202 50-51 0.10589421273055422 2202 52-53 0.11833320890134047 2202 54-55 0.30087689458952127 2202 56-57 0.2637410045197299 2202 58-59 0.2684642078701742 2202 60-61 0.27061503980513635 2202 62-63 0.3307163197763998 2202 64-65 0.27654280385387864 2202 66-67 0.35273420038150505 2202 68-69 0.29614775531074145 2202 70-71 0.3324028687190861 2202 72-73 0.3831913896195118 2202 74-75 0.3455171988646626 2202 76-77 0.2948212012563829 2202 78-79 0.33572081005915066 2202 80-81 0.2654425700757841 2202 82-83 0.3316842327119218 2202 84-85 0.2906965336123548 2202 86-87 0.3408561776380665 2202 88-89 0.4335990587364691 2202 90-91 0.4111161590702892 2202 92-93 0.3119409298025495 2202 94-95 0.3959333681100361 2202 96-97 0.4231274349878973 2202 98-99 0.35544621561967205 2202 100-101 0.4729258956727058 1102 1 0.06603999376064351 1102 2 -0.07330662401195553 1102 3 0.04839441313985304 1102 4 0.0966998617713486 1102 5 0.06338157186506521 1102 6 0.1439801038361992 1102 7 0.1379907156698721 1102 8 0.13169889437423166 1102 9 0.06959022938357862 1102 10-11 0.1432437448865116 1102 12-13 0.1743069445238845 1102 14-15 0.17671010411290666 1102 16-17 0.14518175524635524 1102 18-19 0.16092019338589125 1102 20-21 0.10664736059373325 1102 22-23 0.08022224726347105 1102 24-25 0.0275159791334616 1102 26-27 0.13102086319383943 1102 28-29 0.09920916430789362 1102 30-31 0.11099104161318252 1102 32-33 0.08486859540475677 1102 34-35 0.0392130806328268 1102 36-37 0.09632595518139198 1102 38-39 0.023362626174204593 1102 40-41 0.05055071013206458 1102 42-43 0.06031875577227197 1102 44-45 0.07604478049760388 1102 46-47 0.12342964072169593 1102 48-49 0.06174687780310251 1102 50-51 0.044961772294598745 1102 52-53 0.09663871027363058 1102 54-55 0.24104984651555128 1102 56-57 0.31589106106346065 1102 58-59 0.37493460234583864 1102 60-61 0.3674220357773379 1102 62-63 0.3807190289399145 1102 64-65 0.36470126470566555 1102 66-67 0.35421981445499284 1102 68-69 0.2635683204905277 1102 70-71 0.2736152274402315 1102 72-73 0.32113115928159885 1102 74-75 0.17279783172525498 1102 76-77 0.13436683429938512 1102 78-79 0.17475599377798545 1102 80-81 0.16171755070928384 1102 82-83 0.28529409921721793 1102 84-85 0.2852655995786151 1102 86-87 0.345226916740792 1102 88-89 0.35847744143558913 1102 90-91 0.3437435541317768 1102 92-93 0.333562200545078 1102 94-95 0.3997829285281398 1102 96-97 0.3079647832479182 1102 98-99 0.2449957648792349 1102 100-101 0.26304565679422254 2305 1 0.15904277566964353 2305 2 0.17558182152649948 2305 3 0.1478470106066858 2305 4 0.16964694707227324 2305 5 0.2165294113269809 2305 6 0.25188733182508827 2305 7 0.1849911903172199 2305 8 0.34938905539483045 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sequence quality scores pass #Quality Count 2 2942.0 3 541.0 4 619.0 5 777.0 6 1445.0 7 4511.0 8 17734.0 9 45282.0 10 66384.0 11 55680.0 12 36756.0 13 27819.0 14 23304.0 15 22756.0 16 23358.0 17 23012.0 18 23874.0 19 25607.0 20 27467.0 21 29807.0 22 32099.0 23 35307.0 24 38997.0 25 44505.0 26 51691.0 27 62244.0 28 76606.0 29 94651.0 30 114239.0 31 147292.0 32 202685.0 33 288308.0 34 440932.0 35 753625.0 36 1497802.0 37 2490838.0 38 699110.0 39 597.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.4143225432265 26.217006457036895 22.364746760174423 15.003924239562188 2 32.23718483918224 24.742741609768633 29.73148971471963 13.288583836329494 3 36.43295942870579 20.114990834141043 26.360435833135615 17.09161390401755 4 30.9119239397523 19.030249929113783 30.932365237717534 19.125460893416385 5 31.32143410067051 17.708487952598855 32.69860296007112 18.271474986659516 6 27.59655078028262 18.183217120651136 36.402198469253804 17.818033629812444 7 33.190156340182256 19.248774169427406 28.233571463805042 19.327498026585292 8 29.778873384727127 27.928853551074578 23.621401627742017 18.670871436456274 9 29.601182037339456 27.059070627794497 24.090045934885588 19.24970139998046 10-11 25.060079732403633 28.59583943367559 28.642555562289413 17.701525271631365 12-13 25.572923636929822 25.786644578557155 28.47569772770501 20.164734056808005 14-15 24.33104079581514 26.520619843227948 29.055405549029533 20.092933811927377 16-17 24.323063687788878 27.52293230424197 29.785470234528404 18.368533773440745 18-19 23.634300081346435 27.369589646145332 30.18602804674107 18.810082225767168 20-21 22.935043759431885 28.83072227337039 29.452506493284673 18.781727473913048 22-23 24.46313883532744 27.608777037122255 28.776896226458355 19.151187901091948 24-25 22.788438167914084 28.757927592410425 29.11178282151988 19.341851418155603 26-27 22.296867657698648 29.13517080224149 29.166544572464286 19.401416967595576 28-29 22.443949618398463 29.234007281075304 28.60427392387833 19.7177691766479 30-31 22.7355702213944 28.920061562794984 28.880320009372884 19.46404820643773 32-33 22.614396641433558 28.550411657178838 29.409676600543516 19.42551510084409 34-35 21.643393373543365 28.422211052645846 28.982364493942818 20.952031079867968 36-37 20.442446892509476 29.08270762604027 29.554004018394537 20.920841463055716 38-39 20.581980902866835 29.50883473911488 29.49078752857142 20.418396829446866 40-41 21.575894902324116 28.186787700570985 29.863934117500975 20.37338327960392 42-43 22.36083678436875 28.21795015593888 29.88907569432073 19.532137365371632 44-45 21.087026335792867 29.088177686981826 29.93683622362406 19.887959753601255 46-47 22.141594318988794 29.27871844898767 28.943410965415566 19.63627626660796 48-49 21.39909544023017 28.870711434533348 29.24534493112131 20.48484819411517 50-51 20.778160821040284 28.59687907775704 29.115593429556014 21.50936667164666 52-53 23.033635693566186 28.321648174437957 28.005295335278173 20.63942079671768 54-55 23.60114241451709 28.059101046023127 28.337590231202114 20.00216630825767 56-57 23.8132050216899 27.608900887383747 28.18950130219351 20.388392788732844 58-59 22.787554862235922 27.67285122673362 28.511288025164696 21.028305885865763 60-61 21.148766985653168 27.836993940239964 28.18435438117647 22.8298846929304 62-63 22.211755406070207 27.964871283915784 28.561557511976154 21.261815798037855 64-65 21.281133963974952 28.822010649562284 29.429621710277637 20.467233676185128 66-67 21.44165463375952 28.330102056994278 28.644025704900976 21.584217604345223 68-69 21.16546355860754 27.419693885829222 30.738954658085653 20.675887897477583 70-71 20.95088534252391 29.71849853899753 29.239356326432077 20.091259792046486 72-73 21.060296693374916 31.184633989629535 27.926599024055804 19.82847029293974 74-75 21.86278355341889 30.74459930754462 27.511129698530844 19.881487440505644 76-77 21.982613332495212 29.86268083145011 27.72407305133125 20.430632784723425 78-79 21.690060199703158 29.799988505737176 28.31530401159762 20.19464728296205 80-81 21.127700098715184 30.534893506549555 28.077769876004616 20.25963651873064 82-83 20.484594278482994 30.391955898324618 28.384927561875 20.73852226131739 84-85 20.671444049304764 29.959128090807397 28.53270152434621 20.836726335541627 86-87 20.529929255193153 30.14148828145088 29.02386235044565 20.30472011291032 88-89 20.55533104182764 30.500083343226592 28.896249208372588 20.048336406573174 90-91 21.095506205524586 29.50762013132741 28.700389676328435 20.696483986819565 92-93 20.777472759301393 30.00124347367802 28.150545699788953 21.07073806723163 94-95 20.50792353089415 30.498354769808962 27.748081852333133 21.24563984696376 96-97 20.830398478390077 30.794016265255753 27.466812999899187 20.908772256454984 98-99 21.10332600839363 29.923472108004773 27.815939559889525 21.15726232371207 100-101 21.384222374928953 30.073832750958516 27.313881674838964 21.22806319927356 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3407.0 1 2521.5 2 1354.5 3 1319.0 4 1430.0 5 1323.5 6 1583.5 7 2103.5 8 2548.5 9 2903.0 10 3193.0 11 3554.0 12 3706.5 13 3489.5 14 3196.0 15 2905.0 16 2705.0 17 2570.5 18 2631.5 19 2865.5 20 3284.5 21 4020.5 22 5134.5 23 6879.0 24 9302.0 25 12545.0 26 16718.0 27 21672.0 28 27747.0 29 34971.0 30 43031.5 31 53081.0 32 68375.0 33 90477.5 34 121218.5 35 171199.5 36 239377.5 37 320048.0 38 398905.5 39 437567.0 40 456383.5 41 446670.0 42 422667.0 43 437060.5 44 438536.0 45 430263.0 46 446543.0 47 417298.5 48 334886.5 49 269200.0 50 226513.5 51 201097.0 52 182360.5 53 156242.5 54 123867.0 55 92117.0 56 70292.0 57 58044.0 58 48590.0 59 37503.5 60 27198.5 61 20083.0 62 14588.5 63 10403.5 64 7631.5 65 5367.0 66 3810.5 67 2896.0 68 2132.5 69 1411.5 70 954.5 71 742.0 72 560.0 73 410.0 74 314.0 75 253.5 76 192.5 77 163.5 78 136.5 79 118.0 80 91.5 81 79.5 82 77.5 83 62.5 84 45.5 85 29.5 86 28.5 87 22.5 88 19.0 89 21.0 90 17.0 91 12.0 92 9.0 93 7.5 94 5.5 95 6.0 96 2.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18354305414420513 2 0.18917296479725748 3 0.15927070349849817 4 0.16081096207338988 5 0.1690964909590141 6 0.16620186708551077 7 0.1324622374406851 8 0.11465631719129069 9 0.11416502781826489 10-11 0.0934113713307157 12-13 0.13828468041559894 14-15 0.11621649290292667 16-17 0.10876084471498113 18-19 0.11206045036895168 20-21 0.1042794889475161 22-23 0.1050628963261248 24-25 0.4636311091335607 26-27 0.3098973696499749 28-29 0.4016688967220775 30-31 0.26785893302836217 32-33 0.29590226156432115 34-35 0.31477706815232576 36-37 0.2952250789150153 38-39 0.34374322402410346 40-41 0.33908925307152127 42-43 0.38167209143081127 44-45 0.3351323819049892 46-47 0.3591192535906946 48-49 0.35410677417671516 50-51 0.3697151703386564 52-53 0.36169520327628935 54-55 0.3365464986138337 56-57 0.35263954510321927 58-59 0.3442544305338735 60-61 0.31505590806674577 62-63 0.3241182052853973 64-65 0.32543937535610185 66-67 0.3372236812631395 68-69 0.32393231200911726 70-71 0.31224095274021957 72-73 0.298418459839683 74-75 0.3072284733262402 76-77 0.2967122251252556 78-79 0.3068500477280987 80-81 0.3153015527532587 82-83 0.3416253684836274 84-85 0.33937473203152274 86-87 0.33286846736172165 88-89 0.3464785108036525 90-91 0.33500624003894197 92-93 0.3187737735923464 94-95 0.3112251787662609 96-97 0.29628732620804404 98-99 0.32134972327794115 100-101 0.32450990897470167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7531203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.5793270904244 #Duplication Level Percentage of deduplicated Percentage of total 1 88.0251686953838 38.36077618765916 2 6.147961491470193 5.35848049552226 3 1.7709385801125805 2.315289349193337 4 0.9347564131805753 1.6294422191947273 5 0.5650107016427613 1.2311393088240041 6 0.37350606409086795 0.9766285762363771 7 0.2766586611882436 0.8439618801824956 8 0.18166698878086754 0.6333540100491108 9 0.17008353245302787 0.6670913304118783 >10 1.1821954493046787 10.611954345491045 >50 0.16818826659165648 5.141208673834949 >100 0.17032783421709047 14.94428429908822 >500 0.01990812205337294 6.08642540493799 >1k 0.013260339198161719 9.807598929744676 >5k 2.459989410216508E-4 0.67867492571026 >10k+ 1.2286139108471657E-4 0.7136900639195196 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 18387 0.24414426221149532 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 14056 0.18663684938515135 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10604 0.14080087869096078 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10271 0.13637927433372862 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9792 0.13001906866671897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8362 0.11103139830383009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017261518511717186 0.0 0.0 2 0.001991713674428906 0.0 0.0 3 0.002350222135826109 0.0 1.3278091162859373E-5 4 0.0027352867795490308 0.0 1.3278091162859373E-5 5 0.0032531323349005463 0.0 1.3278091162859373E-5 6 0.0038240902549034994 0.0 1.3278091162859373E-5 7 0.004341935810255015 0.0 1.3278091162859373E-5 8 0.0049925622772351245 0.0 1.3278091162859373E-5 9 0.005762691564680968 0.0 1.3278091162859373E-5 10-11 0.009858982688423085 0.0 1.3278091162859373E-5 12-13 0.01357684821402371 0.0 1.3278091162859373E-5 14-15 0.017739529793580122 0.0 1.991713674428906E-5 16-17 0.02240677883732519 0.0 2.6556182325718747E-5 18-19 0.027339589704327448 0.0 2.6556182325718747E-5 20-21 0.031721359788071044 0.0 2.6556182325718747E-5 22-23 0.036169520327628935 0.0 2.6556182325718747E-5 24-25 0.04173304052486701 0.0 2.6556182325718747E-5 26-27 0.047960465280248055 0.0 2.6556182325718747E-5 28-29 0.05517710782726212 0.0 3.319522790714843E-5 30-31 0.1042130984917018 0.0 6.639045581429686E-5 32-33 0.11530030461268936 0.0 6.639045581429686E-5 34-35 0.12410367905366514 0.0 7.302950139572654E-5 36-37 0.1333120352751081 0.0 7.966854697715623E-5 38-39 0.14358263878957983 0.0 7.966854697715623E-5 40-41 0.15475615250312602 0.0 8.630759255858592E-5 42-43 0.16667987836737372 1.3278091162859373E-5 9.294663814001562E-5 44-45 0.18059531790605032 1.3278091162859373E-5 1.0622472930287499E-4 46-47 0.19518794009403279 1.3278091162859373E-5 1.0622472930287499E-4 48-49 0.21108845426155687 1.3278091162859373E-5 1.0622472930287499E-4 50-51 0.22765951203280538 1.3278091162859373E-5 1.0622472930287499E-4 52-53 0.24610278065801705 1.3278091162859373E-5 1.0622472930287499E-4 54-55 0.2663717868181219 1.3278091162859373E-5 1.0622472930287499E-4 56-57 0.28793540686660546 1.3278091162859373E-5 1.0622472930287499E-4 58-59 0.3123538165151039 1.3278091162859373E-5 1.0622472930287499E-4 60-61 0.3387705788836126 1.3278091162859373E-5 1.0622472930287499E-4 62-63 0.3670329959237588 1.3278091162859373E-5 1.0622472930287499E-4 64-65 0.3976921084188011 1.3278091162859373E-5 1.0622472930287499E-4 66-67 0.43064833068501807 1.3278091162859373E-5 1.0622472930287499E-4 68-69 0.4671630813828813 1.3278091162859373E-5 1.0622472930287499E-4 70-71 0.5061475570370364 1.3278091162859373E-5 1.0622472930287499E-4 72-73 0.5504100739284281 1.3278091162859373E-5 1.0622472930287499E-4 74-75 0.5985697105761192 1.3278091162859373E-5 1.0622472930287499E-4 76-77 0.652445565469421 1.3278091162859373E-5 1.1286377488430466E-4 78-79 0.7137969856874128 1.3278091162859373E-5 1.2614186604716405E-4 80-81 0.7803202224133382 1.3278091162859373E-5 1.3941995721002343E-4 82-83 0.8553746327114008 1.3278091162859373E-5 1.5933709395431247E-4 84-85 0.9385884300290405 1.3278091162859373E-5 1.5933709395431247E-4 86-87 1.0304728208760272 1.3278091162859373E-5 1.5933709395431247E-4 88-89 1.1333116369323732 1.3278091162859373E-5 1.5933709395431247E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4570 0.0 76.70194 2 GAGCGGC 2975 0.0 72.9953 9 CGGGAGA 4075 0.0 70.41176 4 AGAGCGG 3350 0.0 65.95649 8 GTTCGGA 685 0.0 62.320377 2 AAGCGGC 1055 0.0 60.67331 9 TCGGGAA 1560 0.0 58.370586 3 GGTCCGG 445 0.0 55.428486 1 CGGAAAG 1595 0.0 55.008335 5 ATTCGGA 865 0.0 54.83553 2 TCGGGAG 5390 0.0 54.7292 3 GATCGGG 6895 0.0 54.55425 1 AAGCGTC 535 0.0 53.17578 9 GGTTCGG 940 0.0 52.984776 1 GAGAGCG 5025 0.0 51.047867 7 GAATCGG 1075 0.0 48.09586 1 TCGGAGA 1200 0.0 47.030884 4 GATCCGG 1050 0.0 46.07873 1 GAACCGG 445 0.0 45.835094 1 TTGTGTT 59510 0.0 45.31377 1 >>END_MODULE