FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531203
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT183870.24414426221149532Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA140560.18663684938515135No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC106040.14080087869096078No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG102710.13637927433372862No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC97920.13001906866671897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83620.11103139830383009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA45700.076.701942
GAGCGGC29750.072.99539
CGGGAGA40750.070.411764
AGAGCGG33500.065.956498
GTTCGGA6850.062.3203772
AAGCGGC10550.060.673319
TCGGGAA15600.058.3705863
GGTCCGG4450.055.4284861
CGGAAAG15950.055.0083355
ATTCGGA8650.054.835532
TCGGGAG53900.054.72923
GATCGGG68950.054.554251
AAGCGTC5350.053.175789
GGTTCGG9400.052.9847761
GAGAGCG50250.051.0478677
GAATCGG10750.048.095861
TCGGAGA12000.047.0308844
GATCCGG10500.046.078731
GAACCGG4450.045.8350941
TTGTGTT595100.045.313771
Produced by FastQC (version 0.11.2)