FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5918099
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC131840.2227742388222975No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG125130.21143613853029497No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT125120.2114192412124231Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA95580.16150456421901696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC30950.082.2468269
CGGGAGA40800.077.1597064
AGAGCGG34200.075.678418
GAGAGCG38400.071.1056447
GATCGGG55450.068.842691
TCGGGAG48900.066.3177643
AGAGCGT85350.064.759058
ATCGGGA56400.064.65192
GAGCGTC87100.063.0225339
AAGCGGC12450.062.061719
AAGAGCG89400.061.190097
TCGGGAA13100.059.3547063
GTTCGGA7750.059.3477332
CGGAAGA95750.058.57724
TCGGAAG98000.058.5385443
CGGAAAG15850.058.0298465
GATCGGA108450.056.536961
GATCCGG9300.055.578531
ATCGGAA112400.054.3354952
GGTTCGG10450.053.092491
Produced by FastQC (version 0.11.2)