##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6764852 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18971117180391 33.0 33.0 33.0 33.0 33.0 2 32.1388402880063 33.0 33.0 33.0 33.0 33.0 3 32.10675902443985 33.0 33.0 33.0 33.0 33.0 4 35.873879724197955 37.0 37.0 37.0 33.0 37.0 5 35.89646794933577 37.0 37.0 37.0 33.0 37.0 6 35.91448652535192 37.0 37.0 37.0 33.0 37.0 7 35.812894797994105 37.0 37.0 37.0 33.0 37.0 8 35.81630063747145 37.0 37.0 37.0 33.0 37.0 9 35.73073365093575 37.0 37.0 37.0 33.0 37.0 10-11 35.66757779771088 37.0 37.0 37.0 33.0 37.0 12-13 35.65038104307382 37.0 37.0 37.0 33.0 37.0 14-15 37.464511492638714 40.0 37.0 40.0 33.0 40.0 16-17 37.45501934114745 40.0 37.0 40.0 33.0 40.0 18-19 37.46757150045559 40.0 37.0 40.0 33.0 40.0 20-21 37.476351589066546 40.0 37.0 40.0 33.0 40.0 22-23 37.4919922860101 40.0 37.0 40.0 33.0 40.0 24-25 37.25980147089692 40.0 37.0 40.0 33.0 40.0 26-27 37.20529126136093 40.0 37.0 40.0 33.0 40.0 28-29 37.11206231858435 40.0 37.0 40.0 33.0 40.0 30-31 37.13245064341393 40.0 37.0 40.0 33.0 40.0 32-33 37.045980606818894 40.0 37.0 40.0 33.0 40.0 34-35 37.05989680188125 40.0 37.0 40.0 33.0 40.0 36-37 36.95571019144247 40.0 37.0 40.0 33.0 40.0 38-39 36.88762850983288 40.0 37.0 40.0 33.0 40.0 40-41 36.745540478934345 40.0 37.0 40.0 33.0 40.0 42-43 36.6962268354134 40.0 37.0 40.0 33.0 40.0 44-45 36.59239699552924 40.0 37.0 40.0 33.0 40.0 46-47 36.52711345347984 40.0 37.0 40.0 33.0 40.0 48-49 36.428116757025876 40.0 37.0 40.0 33.0 40.0 50-51 35.26682564526171 37.0 35.0 38.5 30.0 38.5 52-53 35.687445490307844 37.0 37.0 38.5 33.0 40.0 54-55 36.48448273517292 38.5 37.0 40.0 33.0 40.0 56-57 36.52137563393848 40.0 37.0 40.0 33.0 40.0 58-59 36.33263706286553 37.0 37.0 40.0 33.0 40.0 60-61 36.15920030475168 37.0 37.0 40.0 33.0 40.0 62-63 35.90736966603261 37.0 37.0 40.0 33.0 40.0 64-65 35.74141215506267 37.0 37.0 40.0 33.0 40.0 66-67 35.518938847442634 37.0 37.0 40.0 27.0 40.0 68-69 35.321049965320746 37.0 37.0 40.0 27.0 40.0 70-71 35.007199418405605 37.0 33.0 37.0 27.0 40.0 72-73 34.78140837375304 37.0 33.0 37.0 27.0 40.0 74-75 34.56335519239741 37.0 33.0 37.0 27.0 40.0 76-77 34.30217490345687 37.0 33.0 37.0 27.0 37.0 78-79 34.039771971360196 37.0 33.0 37.0 27.0 37.0 80-81 33.75669186849912 37.0 33.0 37.0 27.0 37.0 82-83 33.519208550312705 37.0 33.0 37.0 27.0 37.0 84-85 33.3457449623436 37.0 33.0 37.0 27.0 37.0 86-87 33.180956952199395 37.0 33.0 37.0 27.0 37.0 88-89 33.020493944287324 37.0 33.0 37.0 27.0 37.0 90-91 32.86961215116014 37.0 33.0 37.0 27.0 37.0 92-93 32.749308854059194 37.0 33.0 37.0 27.0 37.0 94-95 32.616408755136106 35.0 33.0 37.0 27.0 37.0 96-97 32.45932231776837 33.0 33.0 37.0 22.0 37.0 98-99 32.328489004637504 33.0 33.0 37.0 22.0 37.0 100-101 31.08905176343843 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3106772734930985 2306 2 0.2966229705846217 2306 3 0.2614621082995612 2306 4 0.36166955449883886 2306 5 0.37548312185624155 2306 6 0.36295570371509456 2306 7 0.40941711526966884 2306 8 0.37972930248918146 2306 9 0.3906713480904074 2306 10-11 0.42268854381648424 2306 12-13 0.36861768693017183 2306 14-15 0.6560370157686677 2306 16-17 0.6007030933992681 2306 18-19 0.653023377865054 2306 20-21 0.568736463355421 2306 22-23 0.512719779540177 2306 24-25 0.5089320877281693 2306 26-27 0.5988261950664011 2306 28-29 0.528735918317814 2306 30-31 0.4023084096085583 2306 32-33 0.37982043075794536 2306 34-35 0.39361935005518944 2306 36-37 0.4146552208805261 2306 38-39 0.4566032943874845 2306 40-41 0.43025153370454206 2306 42-43 0.48270749036336724 2306 44-45 0.4414411926271171 2306 46-47 0.3133051167057772 2306 48-49 0.35887739709884414 2306 50-51 0.3694476652084191 2306 52-53 0.3091193352746373 2306 54-55 0.4586885918537362 2306 56-57 0.4279240944388931 2306 58-59 0.5385967107067913 2306 60-61 0.461808105848462 2306 62-63 0.5260885049580608 2306 64-65 0.4890716212350057 2306 66-67 0.5204400981742197 2306 68-69 0.4955477747797161 2306 70-71 0.498609357527684 2306 72-73 0.4282093697614897 2306 74-75 0.3492161438097554 2306 76-77 0.43990190741833857 2306 78-79 0.39023605768250746 2306 80-81 0.4420371008096069 2306 82-83 0.4632654147630788 2306 84-85 0.47174793681978855 2306 86-87 0.5702118932551699 2306 88-89 0.6503897111534584 2306 90-91 0.6902466612069205 2306 92-93 0.44033323331750296 2306 94-95 0.4721204017690894 2306 96-97 0.5025830011796941 2306 98-99 0.3782158734902268 2306 100-101 0.3600344045016932 2307 1 0.23847301489961126 2307 2 0.20049385207020265 2307 3 0.19188086687913852 2307 4 0.30303477732525863 2307 5 0.23056499639115913 2307 6 0.26422632392580425 2307 7 0.30523318280174294 2307 8 0.22052707783954872 2307 9 0.2714713608460997 2307 10-11 0.25731093084209533 2307 12-13 0.3432222335379862 2307 14-15 0.4448556239632282 2307 16-17 0.46130763350540605 2307 18-19 0.4599566872419132 2307 20-21 0.46283621499620864 2307 22-23 0.3605570223385399 2307 24-25 0.31191683596150455 2307 26-27 0.32086276952249193 2307 28-29 0.30948815400740415 2307 30-31 0.32303204613938163 2307 32-33 0.2865834011426145 2307 34-35 0.27297273785372766 2307 36-37 0.22605214355036196 2307 38-39 0.22045114855530556 2307 40-41 0.3241567765964106 2307 42-43 0.179138054868055 2307 44-45 0.18098069064851785 2307 46-47 0.11461219795430111 2307 48-49 0.18843948641228536 2307 50-51 -0.020005600342955177 2307 52-53 0.03830471901158461 2307 54-55 0.11588264718751162 2307 56-57 0.1835805218629858 2307 58-59 0.2067437835732946 2307 60-61 0.21788513038422508 2307 62-63 0.1739144833288151 2307 64-65 0.15572018466443893 2307 66-67 0.16919584332900683 2307 68-69 0.09847182513764352 2307 70-71 0.09239616654755167 2307 72-73 0.1388631704531491 2307 74-75 0.12660334460258582 2307 76-77 0.25128740053839493 2307 78-79 0.2586193997251698 2307 80-81 0.2532865745472108 2307 82-83 0.1929640962264827 2307 84-85 0.09024432304384078 2307 86-87 0.11638472012236178 2307 88-89 0.19297672963077872 2307 90-91 0.34430785888648785 2307 92-93 0.28049797157229506 2307 94-95 0.19521344846961597 2307 96-97 0.1863129880509362 2307 98-99 0.14219943743644592 2307 100-101 -0.026948040071058443 2201 1 0.2403846624108752 2201 2 0.18149144301320774 2201 3 0.1785765552093963 2201 4 0.250303759840655 2201 5 0.20441286359582023 2201 6 0.25598147552457107 2201 7 0.2731025854966518 2201 8 0.24344303894881136 2201 9 0.23768472357588166 2201 10-11 0.26911525124785385 2201 12-13 0.2713761215021222 2201 14-15 0.45927287180098375 2201 16-17 0.38128948070740876 2201 18-19 0.42398973616148794 2201 20-21 0.4193225948301986 2201 22-23 0.39153410402369104 2201 24-25 0.3584384730701231 2201 26-27 0.4352456979620598 2201 28-29 0.3583391150275048 2201 30-31 0.3278983666065187 2201 32-33 0.32178063266891854 2201 34-35 0.2952948765089829 2201 36-37 0.2703827165756749 2201 38-39 0.24550587084021203 2201 40-41 0.3306956585010141 2201 42-43 0.23537397823413642 2201 44-45 0.20647789417431994 2201 46-47 0.2741983047449281 2201 48-49 0.2998375732348464 2201 50-51 0.2773062384190652 2201 52-53 0.21099418657748714 2201 54-55 0.35071048122429715 2201 56-57 0.4472442321366543 2201 58-59 0.3863374386398917 2201 60-61 0.44179657130918315 2201 62-63 0.4825590336736312 2201 64-65 0.3598465740184196 2201 66-67 0.38478175222408595 2201 68-69 0.35298005966578216 2201 70-71 0.38207103392991826 2201 72-73 0.313680535760831 2201 74-75 0.4591589013350159 2201 76-77 0.4670402215684817 2201 78-79 0.42685187195936436 2201 80-81 0.35746733538771736 2201 82-83 0.31708369412371695 2201 84-85 0.35562158158568735 2201 86-87 0.3493102310952878 2201 88-89 0.33874074633475715 2201 90-91 0.38601554633209645 2201 92-93 0.3209519734848101 2201 94-95 0.41973061636154085 2201 96-97 0.3429796827814684 2201 98-99 0.17405831310181696 2201 100-101 0.19420664608388805 1101 1 0.18692871254662435 1101 2 0.13616458712976964 1101 3 0.14898755810811792 1101 4 0.1672304334334811 1101 5 0.17950686852548614 1101 6 0.23222082742011452 1101 7 0.287641452285051 1101 8 0.2853591589367781 1101 9 0.280537207474687 1101 10-11 0.27345602480363596 1101 12-13 0.26897780247951886 1101 14-15 0.43677059573034427 1101 16-17 0.3732907350787329 1101 18-19 0.29577829686579094 1101 20-21 0.4094219536203596 1101 22-23 0.3440169858992306 1101 24-25 0.1608574302533583 1101 26-27 0.2743790205585128 1101 28-29 0.24183322972255183 1101 30-31 0.2522377897738721 1101 32-33 0.19886337821277067 1101 34-35 0.166268730450561 1101 36-37 0.08916163355154083 1101 38-39 0.1790924394496045 1101 40-41 0.19411624521601567 1101 42-43 0.10206959193128284 1101 44-45 0.05002353016903527 1101 46-47 0.06586072434932078 1101 48-49 0.05205327843361829 1101 50-51 0.0481672959048538 1101 52-53 0.09668027810482016 1101 54-55 0.23418114346408458 1101 56-57 0.18896870812011457 1101 58-59 -6.833340865810555E-4 1101 60-61 0.14310905742000557 1101 62-63 0.28492838595781933 1101 64-65 0.22398624454413607 1101 66-67 0.17531878901149867 1101 68-69 0.2180364255726417 1101 70-71 0.24277712705122667 1101 72-73 0.19345765308592888 1101 74-75 0.23878528440907587 1101 76-77 0.16340987609371638 1101 78-79 0.08917035488270386 1101 80-81 0.23455488967029225 1101 82-83 0.20628753569106806 1101 84-85 0.1705580601179406 1101 86-87 0.15628505435245899 1101 88-89 0.1730316887442811 1101 90-91 -0.002546880438110577 1101 92-93 0.13310448791516194 1101 94-95 0.15669422422104162 1101 96-97 0.21936230355826325 1101 98-99 0.11308855131806439 1101 100-101 -0.030606087046329833 2304 1 0.23616807202161993 2304 2 0.1686881061328478 2304 3 0.17309947723339292 2304 4 0.25127079271620545 2304 5 0.2599114080664009 2304 6 0.35054539795857664 2304 7 0.28987306139575253 2304 8 0.29583644903230066 2304 9 0.27811747561914046 2304 10-11 0.2734096651129434 2304 12-13 0.298302746823488 2304 14-15 0.3751161110890635 2304 16-17 0.39006903913388413 2304 18-19 0.37592322213885154 2304 20-21 0.3459973827891005 2304 22-23 0.4213734977765853 2304 24-25 0.2862867453889635 2304 26-27 0.34878827609995255 2304 28-29 0.3177593011437949 2304 30-31 0.27748588731856927 2304 32-33 0.23570359441484356 2304 34-35 0.13267930455921118 2304 36-37 0.151114040205087 2304 38-39 0.1716385331820689 2304 40-41 0.32683354772617434 2304 42-43 0.30973453239079873 2304 44-45 0.2654673827276497 2304 46-47 0.2476674493348554 2304 48-49 0.21675937751452778 2304 50-51 0.15006589603739684 2304 52-53 0.15667191384061852 2304 54-55 0.31179970959301073 2304 56-57 0.37301594815693306 2304 58-59 0.40508193264755477 2304 60-61 0.4548900552171915 2304 62-63 0.3940582438433964 2304 64-65 0.40715313845462475 2304 66-67 0.4019547248965267 2304 68-69 0.4314730255236512 2304 70-71 0.324319065003408 2304 72-73 0.28901985949489983 2304 74-75 0.34107623392385733 2304 76-77 0.3751464173875121 2304 78-79 0.2558926125118006 2304 80-81 0.30893205942582114 2304 82-83 0.268420013143178 2304 84-85 0.2532754602274494 2304 86-87 0.3139181286245858 2304 88-89 0.3910397201946907 2304 90-91 0.22922729516459128 2304 92-93 0.38648569535574495 2304 94-95 0.4001801996826089 2304 96-97 0.4961746746661291 2304 98-99 0.33391122598917633 2304 100-101 0.3997838613919349 2202 1 0.18359336764847 2202 2 0.15648119291071083 2202 3 0.13783884053788853 2202 4 0.19401547838567268 2202 5 0.15847469340190656 2202 6 0.20285158312891127 2202 7 0.24369852528946723 2202 8 0.21774216631670384 2202 9 0.25359788839816844 2202 10-11 0.25936195790529837 2202 12-13 0.22592457929916776 2202 14-15 0.34814283008152813 2202 16-17 0.3580064232519007 2202 18-19 0.2524325562855054 2202 20-21 0.3155405456284299 2202 22-23 0.28691147217916324 2202 24-25 0.2321472942245606 2202 26-27 0.26471861891992887 2202 28-29 0.23064373046890552 2202 30-31 0.20385017320166554 2202 32-33 0.07291498441594513 2202 34-35 0.05036642360313692 2202 36-37 0.06628917667493539 2202 38-39 0.06349740435100415 2202 40-41 0.20228133504065937 2202 42-43 0.10610719410225045 2202 44-45 0.15970921645132563 2202 46-47 0.09589318491152454 2202 48-49 0.1404961505397111 2202 50-51 0.10669378604519153 2202 52-53 0.08641577705827075 2202 54-55 0.17315070769588914 2202 56-57 0.20823056031343867 2202 58-59 0.18749679787963203 2202 60-61 0.1967725639040836 2202 62-63 0.3093172417627059 2202 64-65 0.21751931396361357 2202 66-67 0.20140678215778962 2202 68-69 0.23949148685972688 2202 70-71 0.2083081995607614 2202 72-73 0.22708684261230871 2202 74-75 0.22289483409009847 2202 76-77 0.27482009709098776 2202 78-79 0.2552647849038081 2202 80-81 0.20874948374980562 2202 82-83 0.24330962865413852 2202 84-85 0.29404047172389625 2202 86-87 0.2767077952933832 2202 88-89 0.27572334947688404 2202 90-91 0.34517310350518216 2202 92-93 0.22444516387454883 2202 94-95 0.24796477557845265 2202 96-97 0.23210666251480205 2202 98-99 0.16283665148013426 2202 100-101 0.3894200868370241 1102 1 0.054287262475838816 1102 2 -0.04600798457846622 1102 3 0.02446080625969671 1102 4 0.09974712602756597 1102 5 0.04002298430742002 1102 6 0.12495867503682945 1102 7 0.14924826740801933 1102 8 0.10744647606110647 1102 9 0.07483528826335117 1102 10-11 0.16827913574383757 1102 12-13 0.15109586106964912 1102 14-15 0.18521648026304405 1102 16-17 0.17757727962435865 1102 18-19 0.18514474300376804 1102 20-21 0.15131249914482225 1102 22-23 0.1299736856597633 1102 24-25 0.05652456594404498 1102 26-27 0.18707194674402672 1102 28-29 0.0752070564341949 1102 30-31 -0.007488814375676611 1102 32-33 -0.008810299052086634 1102 34-35 -0.017969222041095634 1102 36-37 0.02699330053865623 1102 38-39 -0.007142615871920555 1102 40-41 0.0636252775699333 1102 42-43 -0.024996662809940062 1102 44-45 -0.01295736511334411 1102 46-47 0.024823161642842706 1102 48-49 0.04293199930897629 1102 50-51 0.03865776102519902 1102 52-53 0.018421617182966088 1102 54-55 0.10851999438435911 1102 56-57 0.23492840118613856 1102 58-59 0.2509114204186673 1102 60-61 0.28371117064997975 1102 62-63 0.25952276029142496 1102 64-65 0.23600556775121362 1102 66-67 0.20376021635938457 1102 68-69 0.17860039054441756 1102 70-71 0.24399859232887877 1102 72-73 0.28085518153325495 1102 74-75 0.1038951409052018 1102 76-77 0.16095176052153448 1102 78-79 0.12450829251707773 1102 80-81 0.22762932028678762 1102 82-83 0.3095289359462612 1102 84-85 0.1779511969273031 1102 86-87 0.17109765595005655 1102 88-89 0.2609318521743873 1102 90-91 0.22125503265552737 1102 92-93 0.23172516400892107 1102 94-95 0.3779078020294975 1102 96-97 0.2643276511312891 1102 98-99 0.2085193906671421 1102 100-101 0.2615286204399929 2305 1 0.15141933908812888 2305 2 0.14106872846798524 2305 3 0.13931778791264549 2305 4 0.17279491803385127 2305 5 0.18261804440635387 2305 6 0.22413376294348808 2305 7 0.16025838800890568 2305 8 0.3003820800227004 2305 9 0.2317936421670055 2305 10-11 0.25058823471314184 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12541.0 15 12478.0 16 12888.0 17 13111.0 18 13740.0 19 15629.0 20 17270.0 21 19276.0 22 21724.0 23 24185.0 24 27950.0 25 32585.0 26 38723.0 27 46781.0 28 58988.0 29 72450.0 30 88879.0 31 115734.0 32 158950.0 33 230578.0 34 352340.0 35 598456.0 36 1182958.0 37 2372352.0 38 1101919.0 39 949.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.41123653615198 26.739516500364406 21.485833292728458 16.363413670755143 2 31.862018628213896 24.587236921578494 29.940471235811337 13.610273214396267 3 36.0967053611896 21.196493343099874 25.44775291771036 17.259048378000166 4 30.932492814580414 20.819259989513753 30.145032128757272 18.10321506714856 5 29.935063165775805 19.514161359414686 32.64127300598961 17.909502468819895 6 26.505909202102234 19.76203563468319 35.45414645154679 18.277908711667777 7 32.014336412456665 19.8942443851469 28.00152352508058 20.089895677315848 8 29.825488619138298 27.41803905874678 23.61403372714526 19.142438594969672 9 27.634892613385098 26.9245170873153 25.551555580025067 19.889034719274534 10-11 23.378177331515396 29.445208577157267 29.924603517220138 17.2520105741072 12-13 23.60721733026167 27.4442885451736 30.13215695342494 18.8163371711398 14-15 22.354811598688062 27.71119448175995 31.1557209944181 18.778272925133884 16-17 22.117586068677056 27.952721267208798 32.06754476778822 17.86214789632593 18-19 21.82553671754498 28.08219472048301 31.973050979249972 18.119217582722033 20-21 21.734633294235653 28.8745328478236 31.186704894195046 18.204128963745706 22-23 22.33162881034289 28.758037376283102 30.421928449398578 18.48840536397543 24-25 21.65035342065747 29.16122933057271 30.454203841806564 18.734213406963256 26-27 21.559941180518152 29.42898953397602 30.341956453652667 18.66911283185316 28-29 21.712536751049512 29.329458246020796 30.117250830389104 18.84075417254059 30-31 21.861976908447584 29.070201106105813 30.2073021957579 18.8605197896887 32-33 21.23861519490368 29.496081953767522 30.402974152097016 18.86232869923178 34-35 22.113458879692836 28.91591968028723 30.24408908445182 18.72653235556811 36-37 21.350197519450763 28.772843920526643 30.783676074773076 19.09328248524952 38-39 21.247131544488152 29.246934837680538 30.571597940186003 18.934335677645308 40-41 21.426864469874044 29.638232196012854 29.723660657797673 19.21124267631543 42-43 21.55328146863862 28.98126433289886 30.234115330408518 19.231338868054003 44-45 21.06823666960689 29.165896876812685 30.458683997179588 19.307182456400845 46-47 21.404785214605127 29.362843054364586 29.843232481672523 19.389139249357765 48-49 21.011691393963165 29.5245715408181 29.798923097442724 19.664813967776006 50-51 20.990423356806414 29.188707458100794 29.84580237829098 19.97506680680181 52-53 21.76653130142037 29.056854887170076 29.32816155992991 19.848452251479646 54-55 21.801748668280656 29.200139198065468 29.303558732638997 19.694553401014883 56-57 21.807711431769842 29.15109358197156 29.382177944899524 19.65901704135907 58-59 21.567343086599518 29.227147397724256 29.460037230627023 19.745472285049203 60-61 20.82284141920728 29.30096304389319 29.394594989003973 20.481600547895553 62-63 21.112567338788292 29.190604135246595 29.478999508550967 20.217829017414143 64-65 20.699193400964596 29.35760929578227 29.76670440164353 20.176492901609603 66-67 20.617220907589676 29.0414900998342 29.68551541939745 20.655773573178678 68-69 20.494066152988992 29.07414099545177 30.384562646356866 20.047230205202368 70-71 20.31339744955819 30.072072545030327 29.558174344107808 20.056355661303677 72-73 20.33158064750745 30.403272938934663 29.157630589007567 20.10751582455032 74-75 20.461138904811786 30.37554528037605 29.083191955078675 20.080123859733494 76-77 20.48790183660831 30.28789348862461 29.13315085896378 20.0910538158033 78-79 20.338276404636506 30.256561171424927 29.40237306274899 20.002789361189578 80-81 20.197276945779986 30.560478630394773 29.260023107246113 19.982221316579135 82-83 20.11583084036363 30.3585819188745 29.25220106114359 20.273386179618278 84-85 20.2583564285086 29.975107201022 29.465465673051234 20.301070697418172 86-87 20.371013268283985 29.94627436319053 29.571357066080807 20.111355302444682 88-89 20.60256521803953 30.22397611095547 29.124549267334345 20.048909403670663 90-91 20.668143367004337 30.06207570390858 29.061809743581907 20.207971185505176 92-93 20.406918222297236 30.216471043981624 29.021852957965976 20.354757775755168 94-95 20.164863602322356 30.278779205564167 28.99304479360536 20.563312398508117 96-97 20.278014483893596 30.52120042448446 28.848069172520724 20.35271591910122 98-99 20.566476754939565 30.48007209768872 28.67399622262809 20.279454924743625 100-101 20.663108323790315 30.29494338485351 28.389963805996103 20.651984485360064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1502.0 1 1229.5 2 763.0 3 475.0 4 390.0 5 471.5 6 664.0 7 1018.0 8 1493.5 9 2009.5 10 2450.0 11 2760.5 12 2807.5 13 2514.0 14 2145.0 15 1833.0 16 1635.0 17 1538.0 18 1474.0 19 1526.5 20 1821.5 21 2278.5 22 3094.0 23 4585.0 24 6764.0 25 10201.5 26 16511.5 27 25422.0 28 36176.0 29 49455.0 30 65725.5 31 84355.0 32 108803.5 33 143035.0 34 184298.5 35 230290.0 36 284647.5 37 342674.0 38 396284.5 39 430317.0 40 446404.5 41 441426.0 42 416098.5 43 394323.5 44 366419.5 45 338715.0 46 322705.5 47 296225.0 48 256008.5 49 217406.5 50 178027.0 51 142871.5 52 115597.5 53 92035.0 54 75397.0 55 59757.5 56 42882.5 57 29632.5 58 21034.5 59 15466.5 60 11272.0 61 8138.5 62 5726.5 63 3857.5 64 2685.5 65 1925.5 66 1361.5 67 977.5 68 763.5 69 565.0 70 403.0 71 344.5 72 294.5 73 222.5 74 185.0 75 163.0 76 129.0 77 101.5 78 88.0 79 79.0 80 62.5 81 54.0 82 54.0 83 56.0 84 46.5 85 27.0 86 25.5 87 23.5 88 18.5 89 13.0 90 15.0 91 15.5 92 10.0 93 6.5 94 5.5 95 4.5 96 3.0 97 2.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1890506991135948 2 0.19450536390153103 3 0.1653103423400837 4 0.16622684428277218 5 0.17484491900192348 6 0.17230236522543285 7 0.1399291514433723 8 0.12059391691052518 9 0.12038696485895035 10-11 0.09823570419574588 12-13 0.14454122573561107 14-15 0.1223604004936102 16-17 0.11431883506098878 18-19 0.11746007155810652 20-21 0.10965502275585631 22-23 0.10995805968851942 24-25 0.47424540847309005 26-27 0.3179374803765108 28-29 0.41094025412529345 30-31 0.2758670847492303 32-33 0.30467037564162525 34-35 0.32409430391086164 36-37 0.304212124670281 38-39 0.352631513594089 40-41 0.3479603101442574 42-43 0.3905628681898732 44-45 0.344331258096999 46-47 0.3681307440281029 48-49 0.3635704077487578 50-51 0.3790622470380727 52-53 0.3704737368977178 54-55 0.34559514384054524 56-57 0.361523060667107 58-59 0.3532449786041143 60-61 0.32463385747389595 62-63 0.33327410562714455 64-65 0.3348853751715485 66-67 0.34626773800816335 68-69 0.33343671081052473 70-71 0.32203217453981253 72-73 0.3077894387046457 74-75 0.31659968318597365 76-77 0.306119040002649 78-79 0.31593448016305453 80-81 0.32440473198822384 82-83 0.3507615539852165 84-85 0.3480490038806466 86-87 0.34144871166434976 88-89 0.35509276477888946 90-91 0.3436143170611863 92-93 0.32809291319307504 94-95 0.3208052445197619 96-97 0.3055647041502164 98-99 0.33014026027472587 100-101 0.33371018316439144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6764852.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.20223682976173 #Duplication Level Percentage of deduplicated Percentage of total 1 91.73432450553966 60.73017476333952 2 4.474431652901364 5.924347679279165 3 1.246726625893779 2.476082740481691 4 0.5991624735365108 1.586635838902797 5 0.36894019962592256 1.2212333235827444 6 0.23995732944859532 0.9531427171915839 7 0.14775605445532172 0.6847246917057666 8 0.13629562024961112 0.7218459944499219 9 0.11711613866152495 0.6978015313431739 >10 0.779076167713472 10.072835165604966 >50 0.09026409810060011 4.133181187075723 >100 0.060857085081774495 8.04754381796826 >500 0.0042359422111299845 1.8667897221432816 >1k 8.33661609299898E-4 0.7427670968710326 >5k 2.2444971194793868E-5 0.14089373006035624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 9482 0.14016566807374353 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010347602578740821 0.0 0.0 2 0.001108671704865088 0.0 0.0 3 0.0011678008624578927 0.0 0.0 4 0.001197365441254295 0.0 0.0 5 0.0014930112292183184 0.0 0.0 6 0.0017590924383859396 0.0 0.0 7 0.002054738226349963 0.0 1.4782289398201173E-5 8 0.0023356017249157855 0.0 1.4782289398201173E-5 9 0.0026164652234816075 0.0 2.9564578796402347E-5 10-11 0.004678594594530671 0.0 2.9564578796402347E-5 12-13 0.006482033901111215 0.0 5.17380128937041E-5 14-15 0.008477642969868372 0.0 5.9129157592804694E-5 16-17 0.010665421800802146 0.0 5.9129157592804694E-5 18-19 0.012971458946921529 1.4782289398201173E-5 6.652030229190527E-5 20-21 0.014826636266395778 1.4782289398201173E-5 7.391144699100586E-5 22-23 0.016467470389596103 1.4782289398201173E-5 7.391144699100586E-5 24-25 0.018389168011362256 1.4782289398201173E-5 7.391144699100586E-5 26-27 0.020643467144587938 1.4782289398201173E-5 8.130259169010644E-5 28-29 0.023688618760617376 1.4782289398201173E-5 1.034760257874082E-4 30-31 0.04300167985936721 1.4782289398201173E-5 1.1825831518560939E-4 32-33 0.04719245890375724 1.4782289398201173E-5 1.1825831518560939E-4 34-35 0.05090281354270574 1.4782289398201173E-5 1.2564945988470996E-4 36-37 0.055248806625776886 1.4782289398201173E-5 1.3304060458381055E-4 38-39 0.06057782195382841 1.4782289398201173E-5 1.4043174928291113E-4 40-41 0.06634291481912687 1.4782289398201173E-5 1.552140386811123E-4 42-43 0.07297277161422008 1.4782289398201173E-5 1.626051833802129E-4 44-45 0.0794843700941277 1.4782289398201173E-5 1.626051833802129E-4 46-47 0.08754810896084644 1.4782289398201173E-5 1.626051833802129E-4 48-49 0.09525707288200835 1.4782289398201173E-5 1.626051833802129E-4 50-51 0.10371993356247852 1.4782289398201173E-5 2.0695205157481643E-4 52-53 0.1133505951054066 1.4782289398201173E-5 2.0695205157481643E-4 54-55 0.12391254088042133 1.4782289398201173E-5 2.0695205157481643E-4 56-57 0.13515447196775332 1.4782289398201173E-5 2.1434319627391702E-4 58-59 0.1476085507857378 1.4782289398201173E-5 2.291254856721182E-4 60-61 0.161289559623773 1.4782289398201173E-5 2.4390777507031936E-4 62-63 0.17622706306065528 1.4782289398201173E-5 2.586900644685205E-4 64-65 0.1940692863642841 1.4782289398201173E-5 2.734723538667217E-4 66-67 0.21383320728967906 1.4782289398201173E-5 2.8086349856582226E-4 68-69 0.2343879806978778 1.4782289398201173E-5 2.8086349856582226E-4 70-71 0.25763313077654915 1.4782289398201173E-5 2.8086349856582226E-4 72-73 0.2842486428380103 1.4782289398201173E-5 2.8086349856582226E-4 74-75 0.3139610445283947 1.4782289398201173E-5 3.252103667604258E-4 76-77 0.3475242326070105 1.4782289398201173E-5 3.473838008577275E-4 78-79 0.3858325355824488 1.4782289398201173E-5 3.547749455568281E-4 80-81 0.4281764035635961 1.4782289398201173E-5 3.547749455568281E-4 82-83 0.47715012833983655 1.4782289398201173E-5 3.547749455568281E-4 84-85 0.5330345734097361 1.4782289398201173E-5 3.547749455568281E-4 86-87 0.5948171519495179 1.4782289398201173E-5 3.547749455568281E-4 88-89 0.6654469306941231 1.4782289398201173E-5 3.6216609025592874E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1480 0.0 54.439095 9 GATCGGG 2805 0.0 52.57309 1 AGAGCGG 1500 0.0 52.4494 8 CGGGAGA 2095 0.0 50.68705 4 GAGAGCG 1905 0.0 46.773453 7 ATCGGGA 3125 0.0 41.5714 2 TTGTGTT 54180 0.0 39.059246 1 AAGCGGC 725 0.0 37.915226 9 GGCGCCG 1470 0.0 37.002464 56-57 CGCCGGA 965 0.0 36.42586 58-59 AGCGGCG 1390 0.0 36.142216 10-11 TCGGGAA 960 0.0 35.554478 3 TGTGTTT 96535 0.0 35.473877 1 CCGGATC 920 0.0 35.366364 60-61 TCGGGAG 3250 0.0 35.007484 3 GGGCGCC 2065 0.0 34.968266 54-55 CGCCGTA 1730 0.0 34.7336 58-59 GTCGCCG 1905 0.0 34.288635 56-57 GATCCGG 560 0.0 33.86943 1 CGGCGTG 635 0.0 33.58645 12-13 >>END_MODULE